EhchA.01043.a

pyruvate dehydrogenase subunit beta

CENTER ID: EhchA.01043.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01043.a.A1.GE27392 Full length( EhchA.01043.a ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01043.a.A1.PS00379 Full length( EhchA.01043.a ) 1 332

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.128
RefSeq: YP_506977.1
UniProt: Q2GHV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTLTVREAL CEAIREEMER DHTVLIMGEE VGEYQGAYKV TQGLLEQFGP DRVIDTPITE HGFAGIGVGA AFAGLKPIVE FMTFNFAMQA IDQIINSAAK TSYMSGGQLN CPIVFRGPNG AAARVGAQHS QCYASWYAHI PGLKVVSPYF AADCKGLLKA AIRDLNPVVF LENEIAYGHK HEIPNEVSTS DYITEIGKAA IVKEGTDITI TAFSLQVKFA LEAAELLAKE GINAEVIDLR TLRPLDTETI LRSIKKTNKI ISIEEGWPYS GIGSEIAALI MEYAFDDLDA PMIRITGKDV PLPYATNLEK LALPQIEDIL EAARALCIRN YR
NT Sequence
ATGAGAACTT TAACTGTACG AGAAGCACTA TGCGAAGCAA TACGTGAAGA AATGGAACGC GACCATACAG TACTAATTAT GGGAGAAGAA GTAGGTGAAT ATCAAGGTGC ATACAAAGTG ACCCAAGGAT TACTTGAACA ATTTGGCCCT GATAGAGTCA TAGATACTCC CATAACTGAA CATGGATTTG CTGGGATAGG GGTAGGTGCT GCATTTGCGG GACTGAAACC TATTGTAGAA TTCATGACTT TCAACTTTGC TATGCAGGCA ATAGATCAAA TTATTAACTC AGCAGCTAAA ACTAGTTACA TGTCTGGAGG ACAATTGAAC TGTCCTATTG TATTTAGAGG CCCCAATGGT GCAGCAGCAA GAGTAGGAGC ACAACATTCT CAATGTTATG CTTCATGGTA TGCACACATC CCTGGATTAA AAGTAGTATC CCCATATTTT GCAGCAGATT GTAAAGGTCT ATTAAAGGCA GCTATAAGGG ATTTAAATCC TGTTGTATTT CTTGAAAATG AGATCGCATA TGGACATAAG CATGAAATAC CAAATGAAGT ATCAACATCA GACTATATAA CCGAAATTGG GAAAGCAGCT ATAGTCAAGG AAGGAACTGA TATCACAATA ACAGCGTTTT CCCTACAAGT TAAATTCGCA CTAGAAGCAG CAGAACTTTT AGCAAAAGAA GGTATAAATG CAGAGGTTAT AGACTTAAGA ACGCTACGCC CTCTTGATAC AGAAACAATA TTACGTTCTA TTAAAAAAAC AAACAAAATT ATTAGCATAG AAGAAGGATG GCCATATTCA GGCATAGGAT CTGAAATAGC AGCATTGATA ATGGAATATG CATTTGATGA TTTAGATGCA CCAATGATAA GAATAACTGG AAAAGATGTA CCATTACCTT ATGCTACAAA CCTTGAAAAG TTAGCATTAC CACAAATTGA AGATATACTA GAAGCAGCAC GTGCTTTATG TATTCGCAAT TATAGA
Details for EhchA.01043.a.A1.GE27392
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 38kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRTLTVREA LCEAIREEME RDHTVLIMGE EVGEYQGAYK VTQGLLEQFG PDRVIDTPIT EHGFAGIGVG AAFAGLKPIV EFMTFNFAMQ AIDQIINSAA KTSYMSGGQL NCPIVFRGPN GAAARVGAQH SQCYASWYAH IPGLKVVSPY FAADCKGLLK AAIRDLNPVV FLENEIAYGH KHEIPNEVST SDYITEIGKA AIVKEGTDIT ITAFSLQVKF ALEAAELLAK EGINAEVIDL RTLRPLDTET ILRSIKKTNK IISIEEGWPY SGIGSEIAAL IMEYAFDDLD APMIRITGKD VPLPYATNLE KLALPQIEDI LEAARALCIR N
Validated NT Sequence
aattgcgaat acataaagca cgtgctgctt ctagtatatc ttcaatttgt ggtaatgcta acttttcaag gtttgtagca taaggtaatg gtacatcttt tccagttatt cttatcattg gtgcatctaa atcatcaaat gcatattcca ttatcaatgc tgctatttca gatcctatgc ctgaatatgg ccatccttct tctatgctaa taattttgtt tgttttttta atagaacgta atattgtttc tgtatcaaga gggcgtagcg ttcttaagtc tataacctct gcatttatac cttcttttgc taaaagttct gctgcttcta gtgcgaattt aacttgtagg gaaaacgctg ttattgtgat atcagttcct tccttgacta tagctgcttt cccaatttcg gttatatagt ctgatgttga tacttcattt ggtatttcat gcttatgtcc atatgcgatc tcattttcaa gaaatacaac aggatttaaa tcccttatag ctgcctttaa tagaccttta caatctgctg caaaatatgg ggatactact tttaatccag ggatgtgtgc ataccatgaa gcataacatt gagaatgttg tgctcctact cttgctgctg caccattggg gcctctaaat acaataggac agttcaattg tcctccagac atgtaactag ttttagctgc tgagttaata atttgatcta ttgcctgcat agcaaagttg aaagtcatga attctacaat aggtttcagt cccgcaaatg cagcacctac ccctatccca gcaaatccat gttcagttat gggagtatct atgactctat cagggccaaa ttgttcaagt aatccttggg tcactttgta tgcaccttga tattcaccta cttcttctcc cataattagt actgtatggt cgcgttccat ttcttcacgt attgcttcgc atagtgcttc tcgtacagtt aaagttctca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRTLTVREA LCEAIREEME RDHTVLIMGE EVGEYQGAYK VTQGLLEQFG PDRVIDTPIT EHGFAGIGVG AAFAGLKPIV EFMTFNFAMQ AIDQIINSAA KTSYMSGGQL NCPIVFRGPN GAAARVGAQH SQCYASWYAH IPGLKVVSPY FAADCKGLLK AAIRDLNPVV FLENEIAYGH KHEIPNEVST SDYITEIGKA AIVKEGTDIT ITAFSLQVKF ALEAAELLAK EGINAEVIDL RTLRPLDTET ILRSIKKTNK IISIEEGWPY SGIGSEIAAL IMEYAFDDLD APMIRITGKD VPLPYATNLE KLALPQIEDI LEAARALCIR NYR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaacttta actgtacgag aagcactatg cgaagcaata cgtgaagaaa tggaacgcga ccatacagta ctaattatgg gagaagaagt aggtgaatat caaggtgcat acaaagtgac ccaaggatta cttgaacaat ttggccctga tagagtcata gatactccca taactgaaca tggatttgct gggatagggg taggtgctgc atttgcggga ctgaaaccta ttgtagaatt catgactttc aactttgcta tgcaggcaat agatcaaatt attaactcag cagctaaaac tagttacatg tctggaggac aattgaactg tcctattgta tttagaggcc ccaatggtgc agcagcaaga gtaggagcac aacattctca atgttatgct tcatggtatg cacacatccc tggattaaaa gtagtatccc catattttgc agcagattgt aaaggtctat taaaggcagc tataagggat ttaaatcctg ttgtatttct tgaaaatgag atcgcatatg gacataagca tgaaatacca aatgaagtat caacatcaga ctatataacc gaaattggga aagcagctat agtcaaggaa ggaactgata tcacaataac agcgttttcc ctacaagtta aattcgcact agaagcagca gaacttttag caaaagaagg tataaatgca gaggttatag acttaagaac gctacgccct cttgatacag aaacaatatt acgttctatt aaaaaaacaa acaaaattat tagcatagaa gaaggatggc catattcagg cataggatct gaaatagcag cattgataat ggaatatgca tttgatgatt tagatgcacc aatgataaga ataactggaa aagatgtacc attaccttat gctacaaacc ttgaaaagtt agcattacca caaattgaag atatactaga agcagcacgt gctttatgta ttcgcaatta tagaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EhchA.01043.a.A1.PS00379
PURIFICATION DATe: 10/20/2009
CONCENTRATION: 28.27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRTLTVREA LCEAIREEME RDHTVLIMGE EVGEYQGAYK VTQGLLEQFG PDRVIDTPIT EHGFAGIGVG AAFAGLKPIV EFMTFNFAMQ AIDQIINSAA KTSYMSGGQL NCPIVFRGPN GAAARVGAQH SQCYASWYAH IPGLKVVSPY FAADCKGLLK AAIRDLNPVV FLENEIAYGH KHEIPNEVST SDYITEIGKA AIVKEGTDIT ITAFSLQVKF ALEAAELLAK EGINAEVIDL RTLRPLDTET ILRSIKKTNK IISIEEGWPY SGIGSEIAAL IMEYAFDDLD APMIRITGKD VPLPYATNLE KLALPQIEDI LEAARALCIR N
Validated NT Sequence
aattgcgaat acataaagca cgtgctgctt ctagtatatc ttcaatttgt ggtaatgcta acttttcaag gtttgtagca taaggtaatg gtacatcttt tccagttatt cttatcattg gtgcatctaa atcatcaaat gcatattcca ttatcaatgc tgctatttca gatcctatgc ctgaatatgg ccatccttct tctatgctaa taattttgtt tgttttttta atagaacgta atattgtttc tgtatcaaga gggcgtagcg ttcttaagtc tataacctct gcatttatac cttcttttgc taaaagttct gctgcttcta gtgcgaattt aacttgtagg gaaaacgctg ttattgtgat atcagttcct tccttgacta tagctgcttt cccaatttcg gttatatagt ctgatgttga tacttcattt ggtatttcat gcttatgtcc atatgcgatc tcattttcaa gaaatacaac aggatttaaa tcccttatag ctgcctttaa tagaccttta caatctgctg caaaatatgg ggatactact tttaatccag ggatgtgtgc ataccatgaa gcataacatt gagaatgttg tgctcctact cttgctgctg caccattggg gcctctaaat acaataggac agttcaattg tcctccagac atgtaactag ttttagctgc tgagttaata atttgatcta ttgcctgcat agcaaagttg aaagtcatga attctacaat aggtttcagt cccgcaaatg cagcacctac ccctatccca gcaaatccat gttcagttat gggagtatct atgactctat cagggccaaa ttgttcaagt aatccttggg tcactttgta tgcaccttga tattcaccta cttcttctcc cataattagt actgtatggt cgcgttccat ttcttcacgt attgcttcgc atagtgcttc tcgtacagtt aaagttctca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRTLTVREA LCEAIREEME RDHTVLIMGE EVGEYQGAYK VTQGLLEQFG PDRVIDTPIT EHGFAGIGVG AAFAGLKPIV EFMTFNFAMQ AIDQIINSAA KTSYMSGGQL NCPIVFRGPN GAAARVGAQH SQCYASWYAH IPGLKVVSPY FAADCKGLLK AAIRDLNPVV FLENEIAYGH KHEIPNEVST SDYITEIGKA AIVKEGTDIT ITAFSLQVKF ALEAAELLAK EGINAEVIDL RTLRPLDTET ILRSIKKTNK IISIEEGWPY SGIGSEIAAL IMEYAFDDLD APMIRITGKD VPLPYATNLE KLALPQIEDI LEAARALCIR NYR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGAACTTTA ACTGTACGAG AAGCACTATG CGAAGCAATA CGTGAAGAAA TGGAACGCGA CCATACAGTA CTAATTATGG GAGAAGAAGT AGGTGAATAT CAAGGTGCAT ACAAAGTGAC CCAAGGATTA CTTGAACAAT TTGGCCCTGA TAGAGTCATA GATACTCCCA TAACTGAACA TGGATTTGCT GGGATAGGGG TAGGTGCTGC ATTTGCGGGA CTGAAACCTA TTGTAGAATT CATGACTTTC AACTTTGCTA TGCAGGCAAT AGATCAAATT ATTAACTCAG CAGCTAAAAC TAGTTACATG TCTGGAGGAC AATTGAACTG TCCTATTGTA TTTAGAGGCC CCAATGGTGC AGCAGCAAGA GTAGGAGCAC AACATTCTCA ATGTTATGCT TCATGGTATG CACACATCCC TGGATTAAAA GTAGTATCCC CATATTTTGC AGCAGATTGT AAAGGTCTAT TAAAGGCAGC TATAAGGGAT TTAAATCCTG TTGTATTTCT TGAAAATGAG ATCGCATATG GACATAAGCA TGAAATACCA AATGAAGTAT CAACATCAGA CTATATAACC GAAATTGGGA AAGCAGCTAT AGTCAAGGAA GGAACTGATA TCACAATAAC AGCGTTTTCC CTACAAGTTA AATTCGCACT AGAAGCAGCA GAACTTTTAG CAAAAGAAGG TATAAATGCA GAGGTTATAG ACTTAAGAAC GCTACGCCCT CTTGATACAG AAACAATATT ACGTTCTATT AAAAAAACAA ACAAAATTAT TAGCATAGAA GAAGGATGGC CATATTCAGG CATAGGATCT GAAATAGCAG CATTGATAAT GGAATATGCA TTTGATGATT TAGATGCACC AATGATAAGA ATAACTGGAA AAGATGTACC ATTACCTTAT GCTACAAACC TTGAAAAGTT AGCATTACCA CAAATTGAAG ATATACTAGA AGCAGCACGT GCTTTATGTA TTCGCAATTA TAGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA