EhchA.01012.a

aspartyl/glutamyl-tRNA amidotransferase subunit B

CENTER ID: EhchA.01012.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01012.a.A1.GE27386 Full length( EhchA.01012.a ) 1 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.709
RefSeq: YP_507609.1
UniProt: Q2GG24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIIKGNRCD WEVVIGLEVH AQVISNSKLF SGASTKTYDA LPNTQVALFD VAMPGMLPVL NEYCVYQAIK TGIALSCKIN KYSAFDRKNY FYPDLPSGYQ ITQFYYPIAT EGKIVLEDHD MKEIRIARIH LEQDAGKSIH EFDKTYIDFN RAGVALMEIV SEPDFRSIEE VAEYLKKLRM ILRFIETCDG DMEKGSLRCD ANVSVKPVGS SELGIRSEIK NLNSIRYVMQ AIEYEANRQV NALENGEIVT QNTLLFDVTS GQTRVIRTKE DAHDYRYFPD PDLFPLKIDD QYIDHVRSSL PELPMQKRER YTNDFSLSKY DADILSSDKD VAIYFEKVAE KHDGKLAASW ITGELFGRLN RLGITIGESS VTAEDLIQLL DLIVNNTISG KIAKQVFDMM FESGKSPALI VSEHGLKQVS DENALSVIVE RVLKNNASKV VEYKQGKEKL FGYFVGQVMK ETQGKANPDM VNSIIKQQLE N
NT Sequence
ATGACGATTA TTAAAGGAAA TAGGTGTGAT TGGGAGGTAG TAATAGGGTT AGAAGTCCAT GCACAAGTTA TTTCCAATTC TAAATTATTT TCTGGTGCTT CAACAAAGAC TTATGATGCT TTACCTAATA CGCAAGTAGC GCTTTTTGAT GTAGCAATGC CAGGTATGTT GCCTGTACTT AATGAGTATT GTGTTTATCA AGCAATAAAA ACAGGAATTG CTCTATCATG TAAGATTAAC AAGTATTCAG CTTTTGATAG AAAGAATTAT TTTTATCCTG ACTTACCTTC TGGTTATCAA ATAACACAGT TCTATTATCC TATAGCAACA GAAGGTAAAA TTGTCTTAGA AGATCATGAT ATGAAGGAAA TTCGCATTGC TAGAATTCAT CTTGAGCAAG ATGCAGGAAA AAGTATTCAT GAATTTGACA AAACTTATAT AGATTTCAAT AGGGCAGGTG TTGCATTAAT GGAAATAGTG TCAGAGCCTG ATTTCAGATC TATTGAAGAG GTTGCAGAAT ATCTGAAGAA GTTACGTATG ATATTGCGTT TTATAGAAAC ATGTGATGGA GATATGGAAA AAGGTTCTTT ACGTTGTGAT GCAAATGTTT CAGTCAAACC TGTTGGAAGT AGTGAGCTTG GAATAAGGTC TGAAATAAAA AATCTTAATT CCATTCGTTA TGTTATGCAA GCTATCGAGT ATGAAGCAAA TAGACAGGTT AATGCTTTGG AAAATGGAGA AATAGTTACT CAAAATACGT TGTTGTTTGA TGTTACTTCA GGGCAAACAA GAGTAATTAG GACTAAAGAA GATGCTCATG ATTATCGATA TTTTCCAGAT CCTGACTTAT TTCCATTAAA GATTGATGAT CAATATATAG ATCATGTCAG GTCGTCTTTA CCAGAACTAC CTATGCAGAA AAGAGAGCGT TATACTAATG ATTTTAGTCT AAGTAAGTAT GATGCAGATA TTTTATCTTC TGATAAGGAT GTAGCTATAT ATTTTGAAAA GGTTGCAGAG AAGCATGATG GCAAACTTGC AGCATCTTGG ATAACAGGTG AGCTTTTTGG TAGACTAAAT AGGCTTGGAA TTACTATTGG TGAATCTTCT GTGACAGCTG AAGACTTGAT ACAGCTGCTT GATTTGATAG TCAATAATAC TATTTCTGGA AAAATAGCAA AGCAAGTGTT TGATATGATG TTTGAGTCAG GTAAATCACC TGCATTGATA GTCAGTGAAC ATGGGTTAAA GCAGGTAAGC GATGAGAATG CTTTATCTGT AATTGTGGAA AGGGTTTTAA AAAATAATGC AAGTAAAGTA GTAGAATATA AACAAGGTAA AGAAAAATTA TTTGGGTATT TTGTTGGGCA GGTAATGAAA GAAACGCAAG GCAAAGCAAA CCCTGATATG GTAAATTCTA TTATCAAGCA GCAGTTAGAA AAT
Details for EhchA.01012.a.A1.GE27386
HARVESTED ON: 9/15/2009
SEQUENCED ON: 10/20/2009
EXPECTED MW: 56kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 95
Validated AA Sequence
MTIIKGNRCD REVVIGLEVH AQVISNSKLF SGASTKTYDA LPNTXVALFD VXMPGMLPVL NXYCVYQAIX TGIALSCKIN KYSXFDXKNY FYPDLPSGYQ ITQFYYPIAT XXKIVLXDHD MKEIRIARIH LEQDAXKSIH EFDKTYIDFN RXXVALMEIX SXPXFXSIEX XAEYLKKLXM ILRFIETCDX DMEKXSLRCD ANVSVKPVGS SELGIRXEXK NXNSIRYVMQ XIEYEANRQV XAXENGEIVT QNTXLXDVTS GQTRVIRTKE DAHDYRYFPD PDLFPLKIDD QYIDHVRSSL PELPMQKRER YTNDFSLSKY DADILSSDKD VAIYFEKVAE KHDGKLAASW ITGELFGRLN RLGITIGESS VTAEDLIQLL DLIVNNTISG KIAKQVFDMM FESGKSPALI VSEHGLKQVS DENALSVIVE RVLKNNASKV VEYKQGKEKL FGYFVGQVMK ETQGKANPDM VNSIIKQQL
Validated NT Sequence
taccctggaa gctcanaccc agggtcctgg ttcgatgacg attattaaag gaaataggtg tgatcgggag gtagtaatag ggttagaagt ccatgcacaa gttatttcca attctaaatt attttctggt gcttcaacaa agacttatga tgctttacct aatacncang tagcgctttt tgatgtanca atgccaggta tgttgcctgt acttaatgan tattgtgttt atcaagcaat anaaacagga attgctctat catgtaagat taacaagtat tcnncttttg atanaaagaa ttatttttat cctgacttac cttctggtta tcaaataaca cagttctatt atcctatagc aacnnaangt aaaattgtct tanaagatca tgatatgaag gaaattcgca ttgctagaat tcatcttgag caagatgcan gaaaaagtat tcatgaattt gacaaaactt atatagattt caataggnnn ngtgttgcat taatggaaat antgtcnnag cctganttca natctattga agangnngca gaatatctga agaagttacn tatgatattg cgttttatag aaacatgtga tnnagatatg gaaaaagntt ctttacgttg tgatgcaaat gtttcagtca aacctgttgg aagtagtgag ctnggaataa ggtntgaant aaaaaatntn aattccattc gttatgttat gcaagntatc gagtatgaag caaatagaca ggtnnatgct tnggaaaatg gagaaatagt tactcaaaat acgtngttgt tngatgttac ttcagggcaa acaagagtaa ttaggactaa agaagatgct catgattatc gatattttcc agatcctgac ttatttccat taaagattga tgatcaatat atagatcatg tcaggtcgtc tttaccagaa ctacctatgc agaaaagaga gcgttatact aatgatttta gtctaagtaa gtatgatgca gatattttat cttctgataa ggatgtagct atatattttg aaaaggttgc agagaagcat gatggcaaac ttgcagcatc ttggataaca ggtgagcttt ttggtagact aaataggctt ggaattacta ttggtgaatc ttctgtgaca gctgaagact tgatacagct gcttgatttg atagtcaata atactatttc tggaaaaata gcaaagcaag tgtttgatat gatgtttgag tcaggtaaat cacctgcatt gatagtcagt gaacatgggt taaagcaggt aagcgatgag aatgctttat ctgtaattgt ggaaagggtt ttaaaaaata atgcaagtaa agtagtagaa tataaacaag gtaaagaaaa attatttggg tattttgttg ggcaggtaat gaaagaaacg caaggcaaag caaaccctga tatggtaaat tctattatca agcagcagtt ag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIIKGNRC DWEVVIGLEV HAQVISNSKL FSGASTKTYD ALPNTQVALF DVAMPGMLPV LNEYCVYQAI KTGIALSCKI NKYSAFDRKN YFYPDLPSGY QITQFYYPIA TEGKIVLEDH DMKEIRIARI HLEQDAGKSI HEFDKTYIDF NRAGVALMEI VSEPDFRSIE EVAEYLKKLR MILRFIETCD GDMEKGSLRC DANVSVKPVG SSELGIRSEI KNLNSIRYVM QAIEYEANRQ VNALENGEIV TQNTLLFDVT SGQTRVIRTK EDAHDYRYFP DPDLFPLKID DQYIDHVRSS LPELPMQKRE RYTNDFSLSK YDADILSSDK DVAIYFEKVA EKHDGKLAAS WITGELFGRL NRLGITIGES SVTAEDLIQL LDLIVNNTIS GKIAKQVFDM MFESGKSPAL IVSEHGLKQV SDENALSVIV ERVLKNNASK VVEYKQGKEK LFGYFVGQVM KETQGKANPD MVNSIIKQQL EN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgattatt aaaggaaata ggtgtgattg ggaggtagta atagggttag aagtccatgc acaagttatt tccaattcta aattattttc tggtgcttca acaaagactt atgatgcttt acctaatacg caagtagcgc tttttgatgt agcaatgcca ggtatgttgc ctgtacttaa tgagtattgt gtttatcaag caataaaaac aggaattgct ctatcatgta agattaacaa gtattcagct tttgatagaa agaattattt ttatcctgac ttaccttctg gttatcaaat aacacagttc tattatccta tagcaacaga aggtaaaatt gtcttagaag atcatgatat gaaggaaatt cgcattgcta gaattcatct tgagcaagat gcaggaaaaa gtattcatga atttgacaaa acttatatag atttcaatag ggcaggtgtt gcattaatgg aaatagtgtc agagcctgat ttcagatcta ttgaagaggt tgcagaatat ctgaagaagt tacgtatgat attgcgtttt atagaaacat gtgatggaga tatggaaaaa ggttctttac gttgtgatgc aaatgtttca gtcaaacctg ttggaagtag tgagcttgga ataaggtctg aaataaaaaa tcttaattcc attcgttatg ttatgcaagc tatcgagtat gaagcaaata gacaggttaa tgctttggaa aatggagaaa tagttactca aaatacgttg ttgtttgatg ttacttcagg gcaaacaaga gtaattagga ctaaagaaga tgctcatgat tatcgatatt ttccagatcc tgacttattt ccattaaaga ttgatgatca atatatagat catgtcaggt cgtctttacc agaactacct atgcagaaaa gagagcgtta tactaatgat tttagtctaa gtaagtatga tgcagatatt ttatcttctg ataaggatgt agctatatat tttgaaaagg ttgcagagaa gcatgatggc aaacttgcag catcttggat aacaggtgag ctttttggta gactaaatag gcttggaatt actattggtg aatcttctgt gacagctgaa gacttgatac agctgcttga tttgatagtc aataatacta tttctggaaa aatagcaaag caagtgtttg atatgatgtt tgagtcaggt aaatcacctg cattgatagt cagtgaacat gggttaaagc aggtaagcga tgagaatgct ttatctgtaa ttgtggaaag ggttttaaaa aataatgcaa gtaaagtagt agaatataaa caaggtaaag aaaaattatt tgggtatttt gttgggcagg taatgaaaga aacgcaaggc aaagcaaacc ctgatatggt aaattctatt atcaagcagc agttagaaaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa