SSGCID: Target Page

EhchA.01000.a

ABC transporter, ATP-binding protein

CENTER ID:	EhchA.01000.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01000.a.A1.GE27389	Full length( EhchA.01000.a )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.73
RefSeq:	YP_506915.1
UniProt:	Q2GI18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNYISLSDLH IDFHGHQVLK GISLDIAYGE SLVIVGESGS GKSVLTKTIL GLIAPTSGKV IVSGVDIRHD KSLIKNFGVL FQNCALFDSL TVCENIAFNL HYRFNRDKQL SRKIAASGLN LVGLSSDVLD RYPIELSGGM KKRIALARAI VSEPKIMILD EPTSGLDPIM SDVVNEIIIK CHKEFNLTVI TITHDINSAF KIASKIAVLY DGKIVACESV QDIKNIDNQY VRNFICRN

NT Sequence

ATGAATTATA TTTCTTTATC TGATTTGCAT ATAGACTTTC ATGGACATCA AGTATTGAAG GGAATAAGTC TTGATATTGC ATATGGGGAA TCTTTGGTTA TTGTTGGAGA ATCAGGTAGT GGAAAGTCTG TGTTAACGAA AACAATACTG GGGTTAATAG CTCCTACTTC TGGTAAAGTT ATTGTAAGTG GTGTTGATAT TAGACATGAT AAGAGTTTGA TTAAAAATTT TGGAGTTTTA TTTCAAAATT GTGCGTTGTT TGATAGTTTA ACAGTGTGTG AAAATATTGC TTTTAACTTA CATTATAGGT TCAATCGTGA TAAACAATTA TCGAGGAAAA TTGCTGCTTC TGGATTGAAT TTGGTTGGGT TAAGTAGTGA TGTGTTAGAT AGGTATCCTA TTGAATTATC TGGAGGTATG AAGAAGAGAA TAGCACTAGC TAGAGCTATT GTTAGTGAAC CTAAAATTAT GATATTGGAT GAACCAACTT CAGGCTTAGA TCCGATAATG TCTGATGTTG TGAATGAAAT TATAATAAAA TGTCACAAGG AATTTAATTT AACAGTTATT ACTATTACCC ATGATATTAA TAGTGCTTTT AAAATTGCAA GCAAAATAGC TGTATTGTAT GACGGAAAAA TTGTTGCATG TGAATCTGTG CAAGATATAA AAAATATAGA TAATCAGTAT GTTAGGAACT TTATATGTCG AAAT

Details for EhchA.01000.a.A1.GE27389

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNYISLSDL HIDFHGHQVL KGISLDIAYG ESLVIVGESG SGKSVLTKTI LGLIAPTSGK VIVSGVDIRH DKSLIKNFGV LFQNCALFDS LTVCENIAFN LHYRFNRDKQ LSRKIAASGL NLVGLSSDVL DRYPIELSGG MKKRIALARA IVSEPKIMIL DEPTSGLDPI MSDVVNEIII KCHKEFNLTV ITITHDINSA FKIASKIAVL YDGKIVACES VQDIKNIDNQ YVRNFICRN

Validated NT Sequence

ttattaattt cgacatataa agttcctaac atactgatta tctatatttt ttatatcttg cacagattca catgcaacaa tttttccgtc atacaataca gctattttgc ttgcaatttt aaaagcacta ttaatatcat gggtaatagt aataactgtt aaattaaatt ccttgtgaca ttttattata atttcattca caacatcaga cattatcgga tctaagcctg aagttggttc atccaatatc ataattttag gttcactaac aatagctcta gctagtgcta ttctcttctt catacctcca gataattcaa taggatacct atctaacaca tcactactta acccaaccaa attcaatcca gaagcagcaa ttttcctcga taattgttta tcacgattga acctataatg taagttaaaa gcaatatttt cacacactgt taaactatca aacaacgcac aattttgaaa taaaactcca aaatttttaa tcaaactctt atcatgtcta atatcaacac cacttacaat aactttacca gaagtaggag ctattaaccc cagtattgtt ttcgttaaca cagactttcc actacctgat tctccaacaa taaccaaaga ttccccatat gcaatatcaa gacttattcc cttcaatact tgatgtccat gaaagtctat atgcaaatca gataaagaaa tataattcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaattatatt tctttatctg atttgcatat agactttcat ggacatcaag tattgaaggg aataagtctt gatattgcat atggggaatc tttggttatt gttggagaat caggtagtgg aaagtctgtg ttaacgaaaa caatactggg gttaatagct cctacttctg gtaaagttat tgtaagtggt gttgatatta gacatgataa gagtttgatt aaaaattttg gagttttatt tcaaaattgt gcgttgtttg atagtttaac agtgtgtgaa aatattgctt ttaacttaca ttataggttc aatcgtgata aacaattatc gaggaaaatt gctgcttctg gattgaattt ggttgggtta agtagtgatg tgttagatag gtatcctatt gaattatctg gaggtatgaa gaagagaata gcactagcta gagctattgt tagtgaacct aaaattatga tattggatga accaacttca ggcttagatc cgataatgtc tgatgttgtg aatgaaatta taataaaatg tcacaaggaa tttaatttaa cagttattac tattacccat gatattaata gtgcttttaa aattgcaagc aaaatagctg tattgtatga cggaaaaatt gttgcatgtg aatctgtgca agatataaaa aatatagata atcagtatgt taggaacttt atatgtcgaa ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa