EhchA.00979.a

delta-aminolevulinic acid dehydratase

CENTER ID: EhchA.00979.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00979.a.A1.GE27384 Full length( EhchA.00979.a ) 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00979.a.A1.PW27377 Full length( EhchA.00979.a ) 1 331
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127486
PATRIC ID: fig|205920.11.peg.715
RefSeq: YP_507617.1
UniProt: Q2GG16
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFPNTRLRR KRSNEWLRNL VRETSLSIHD LILPLFVHDR DGVSEPVKQL PDIKRYSIPE LLKVVEHAAN LGINAISLFP VVETHLKSEN AEEAFNPNNL ICRAIKEVKK HIPTIGIIAD VALDPYTISG HDGIVINKQI DNDATLEVLY KQALVLAAAG CDIVAPSDMM DGRVKKIRSA LDNCNFQDVC ILSYSVKYCS SFYKPFREAV GSRSISQTID KSTYQLDIAN INEAILEAQM DIDESADIIM IKPGMPYLDV IKSISSRCQI PVFAYQVSGE YAMIKAASQN HLLDYDSVIY ESLLGFKRAG AKSIITYAAI DAANILIKNK M
NT Sequence
ATGCAATTTC CTAATACTAG ATTAAGACGT AAACGTTCAA ATGAGTGGTT ACGTAATTTA GTAAGAGAAA CTAGTTTATC TATACACGAC CTAATATTAC CACTGTTTGT ACATGATCGT GATGGGGTAT CAGAACCTGT AAAACAATTA CCTGACATCA AAAGATACTC TATCCCTGAA TTATTAAAAG TGGTAGAACA TGCTGCAAAT TTAGGCATAA ATGCTATATC GTTGTTTCCA GTAGTAGAAA CCCATTTGAA ATCTGAAAAT GCAGAAGAAG CATTTAACCC TAATAATCTA ATATGCAGAG CAATAAAAGA AGTCAAAAAA CACATACCAA CTATTGGCAT AATAGCCGAT GTTGCATTAG ATCCTTATAC AATTTCAGGA CATGATGGAA TTGTAATCAA CAAGCAAATT GATAATGACG CAACCTTAGA AGTTTTATAT AAGCAAGCTT TAGTCTTAGC AGCAGCAGGT TGTGACATTG TAGCACCATC AGATATGATG GATGGAAGAG TCAAAAAAAT CAGAAGTGCT TTAGATAACT GTAATTTCCA AGATGTATGC ATACTATCTT ACTCTGTAAA ATACTGTTCC AGTTTTTATA AACCATTTCG TGAAGCTGTA GGATCTCGCT CAATATCCCA AACAATAGAT AAAAGTACAT ACCAATTAGA TATTGCAAAC ATAAATGAAG CAATACTTGA GGCACAAATG GATATCGATG AAAGTGCAGA TATTATTATG ATAAAACCAG GGATGCCATA TCTAGATGTA ATTAAGTCCA TTAGTAGCAG ATGTCAGATA CCAGTATTTG CATACCAAGT AAGTGGAGAA TATGCAATGA TTAAAGCTGC ATCACAAAAT CACTTGCTAG ATTATGATTC TGTTATCTAC GAATCTCTAT TAGGATTTAA GCGTGCAGGT GCAAAAAGTA TAATTACTTA CGCTGCAATA GATGCAGCAA ATATACTCAT AAAAAACAAA ATG
Details for EhchA.00979.a.A1.GE27384
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQFPNTRLR RKRSNEWLRN LVRETSLSIH DLILPLFVHD RDGVSEPVKQ LPDIKRYSIP ELLKVVEHAA NLGINAISLF PVVETHLKSE NAEEAFNPNN LICRAIKEVK KHIPTIGIIA DVALDPYTIS GHDGIVINKQ IDNDATLEVL YKQALVLAAA GCDIVAPSDM MDGRVKKIRS ALDNCNFQDV CILSYSVKYC SSFYKPFREA VGSRSISQTI DKSTYQLDIA NINEAILEAQ MDIDESADII MIKPGMPYLD VIKSISSRCQ IPVFAYQVSG EYAMIKAASQ NHLLDYDSVI YESLLGFKRA GAKSIITYAA IDAANILIKN
Validated NT Sequence
tgttttttat gagtatattt gctgcatcta ttgcagcgta agtaattata ctttttgcac ctgcacgctt aaatcctaat agagattcgt agataacaga atcataatct agcaagtgat tttgtgatgc agctttaatc attgcatatt ctccacttac ttggtatgca aatactggta tctgacatct gctactaatg gacttaatta catctagata tggcatccct ggttttatca taataatatc tgcactttca tcgatatcca tttgtgcctc aagtattgct tcatttatgt ttgcaatatc taattggtat gtacttttat ctattgtttg ggatattgag cgagatccta cagcttcacg aaatggttta taaaaactgg aacagtattt tacagagtaa gatagtatgc atacatcttg gaaattacag ttatctaaag cacttctgat ttttttgact cttccatcca tcatatctga tggtgctaca atgtcacaac ctgctgctgc taagactaaa gcttgcttat ataaaacttc taaggttgcg tcattatcaa tttgcttgtt gattacaatt ccatcatgtc ctgaaattgt ataaggatct aatgcaacat cggctattat gccaatagtt ggtatgtgtt ttttgacttc ttttattgct ctgcatatta gattattagg gttaaatgct tcttctgcat tttcagattt caaatgggtt tctactactg gaaacaacga tatagcattt atgcctaaat ttgcagcatg ttctaccact tttaataatt cagggataga gtatcttttg atgtcaggta attgttttac aggttctgat accccatcac gatcatgtac aaacagtggt aatattaggt cgtgtataga taaactagtt tctcttacta aattacgtaa ccactcattt gaacgtttac gtcttaatct agtattagga aattgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQFPNTRLR RKRSNEWLRN LVRETSLSIH DLILPLFVHD RDGVSEPVKQ LPDIKRYSIP ELLKVVEHAA NLGINAISLF PVVETHLKSE NAEEAFNPNN LICRAIKEVK KHIPTIGIIA DVALDPYTIS GHDGIVINKQ IDNDATLEVL YKQALVLAAA GCDIVAPSDM MDGRVKKIRS ALDNCNFQDV CILSYSVKYC SSFYKPFREA VGSRSISQTI DKSTYQLDIA NINEAILEAQ MDIDESADII MIKPGMPYLD VIKSISSRCQ IPVFAYQVSG EYAMIKAASQ NHLLDYDSVI YESLLGFKRA GAKSIITYAA IDAANILIKN KM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatttcct aatactagat taagacgtaa acgttcaaat gagtggttac gtaatttagt aagagaaact agtttatcta tacacgacct aatattacca ctgtttgtac atgatcgtga tggggtatca gaacctgtaa aacaattacc tgacatcaaa agatactcta tccctgaatt attaaaagtg gtagaacatg ctgcaaattt aggcataaat gctatatcgt tgtttccagt agtagaaacc catttgaaat ctgaaaatgc agaagaagca tttaacccta ataatctaat atgcagagca ataaaagaag tcaaaaaaca cataccaact attggcataa tagccgatgt tgcattagat ccttatacaa tttcaggaca tgatggaatt gtaatcaaca agcaaattga taatgacgca accttagaag ttttatataa gcaagcttta gtcttagcag cagcaggttg tgacattgta gcaccatcag atatgatgga tggaagagtc aaaaaaatca gaagtgcttt agataactgt aatttccaag atgtatgcat actatcttac tctgtaaaat actgttccag tttttataaa ccatttcgtg aagctgtagg atctcgctca atatcccaaa caatagataa aagtacatac caattagata ttgcaaacat aaatgaagca atacttgagg cacaaatgga tatcgatgaa agtgcagata ttattatgat aaaaccaggg atgccatatc tagatgtaat taagtccatt agtagcagat gtcagatacc agtatttgca taccaagtaa gtggagaata tgcaatgatt aaagctgcat cacaaaatca cttgctagat tatgattctg ttatctacga atctctatta ggatttaagc gtgcaggtgc aaaaagtata attacttacg ctgcaataga tgcagcaaat atactcataa aaaacaaaat gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.00979.a.A1.PW27377
PURIFICATION DATe: 10/5/2009
CONCENTRATION: 20.72mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQFPNTRLR RKRSNEWLRN LVRETSLSIH DLILPLFVHD RDGVSEPVKQ LPDIKRYSIP ELLKVVEHAA NLGINAISLF PVVETHLKSE NAEEAFNPNN LICRAIKEVK KHIPTIGIIA DVALDPYTIS GHDGIVINKQ IDNDATLEVL YKQALVLAAA GCDIVAPSDM MDGRVKKIRS ALDNCNFQDV CILSYSVKYC SSFYKPFREA VGSRSISQTI DKSTYQLDIA NINEAILEAQ MDIDESADII MIKPGMPYLD VIKSISSRCQ IPVFAYQVSG EYAMIKAASQ NHLLDYDSVI YESLLGFKRA GAKSIITYAA IDAANILIKN
Validated NT Sequence
tgttttttat gagtatattt gctgcatcta ttgcagcgta agtaattata ctttttgcac ctgcacgctt aaatcctaat agagattcgt agataacaga atcataatct agcaagtgat tttgtgatgc agctttaatc attgcatatt ctccacttac ttggtatgca aatactggta tctgacatct gctactaatg gacttaatta catctagata tggcatccct ggttttatca taataatatc tgcactttca tcgatatcca tttgtgcctc aagtattgct tcatttatgt ttgcaatatc taattggtat gtacttttat ctattgtttg ggatattgag cgagatccta cagcttcacg aaatggttta taaaaactgg aacagtattt tacagagtaa gatagtatgc atacatcttg gaaattacag ttatctaaag cacttctgat ttttttgact cttccatcca tcatatctga tggtgctaca atgtcacaac ctgctgctgc taagactaaa gcttgcttat ataaaacttc taaggttgcg tcattatcaa tttgcttgtt gattacaatt ccatcatgtc ctgaaattgt ataaggatct aatgcaacat cggctattat gccaatagtt ggtatgtgtt ttttgacttc ttttattgct ctgcatatta gattattagg gttaaatgct tcttctgcat tttcagattt caaatgggtt tctactactg gaaacaacga tatagcattt atgcctaaat ttgcagcatg ttctaccact tttaataatt cagggataga gtatcttttg atgtcaggta attgttttac aggttctgat accccatcac gatcatgtac aaacagtggt aatattaggt cgtgtataga taaactagtt tctcttacta aattacgtaa ccactcattt gaacgtttac gtcttaatct agtattagga aattgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQFPNTRLR RKRSNEWLRN LVRETSLSIH DLILPLFVHD RDGVSEPVKQ LPDIKRYSIP ELLKVVEHAA NLGINAISLF PVVETHLKSE NAEEAFNPNN LICRAIKEVK KHIPTIGIIA DVALDPYTIS GHDGIVINKQ IDNDATLEVL YKQALVLAAA GCDIVAPSDM MDGRVKKIRS ALDNCNFQDV CILSYSVKYC SSFYKPFREA VGSRSISQTI DKSTYQLDIA NINEAILEAQ MDIDESADII MIKPGMPYLD VIKSISSRCQ IPVFAYQVSG EYAMIKAASQ NHLLDYDSVI YESLLGFKRA GAKSIITYAA IDAANILIKN KM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATTTCCT AATACTAGAT TAAGACGTAA ACGTTCAAAT GAGTGGTTAC GTAATTTAGT AAGAGAAACT AGTTTATCTA TACACGACCT AATATTACCA CTGTTTGTAC ATGATCGTGA TGGGGTATCA GAACCTGTAA AACAATTACC TGACATCAAA AGATACTCTA TCCCTGAATT ATTAAAAGTG GTAGAACATG CTGCAAATTT AGGCATAAAT GCTATATCGT TGTTTCCAGT AGTAGAAACC CATTTGAAAT CTGAAAATGC AGAAGAAGCA TTTAACCCTA ATAATCTAAT ATGCAGAGCA ATAAAAGAAG TCAAAAAACA CATACCAACT ATTGGCATAA TAGCCGATGT TGCATTAGAT CCTTATACAA TTTCAGGACA TGATGGAATT GTAATCAACA AGCAAATTGA TAATGACGCA ACCTTAGAAG TTTTATATAA GCAAGCTTTA GTCTTAGCAG CAGCAGGTTG TGACATTGTA GCACCATCAG ATATGATGGA TGGAAGAGTC AAAAAAATCA GAAGTGCTTT AGATAACTGT AATTTCCAAG ATGTATGCAT ACTATCTTAC TCTGTAAAAT ACTGTTCCAG TTTTTATAAA CCATTTCGTG AAGCTGTAGG ATCTCGCTCA ATATCCCAAA CAATAGATAA AAGTACATAC CAATTAGATA TTGCAAACAT AAATGAAGCA ATACTTGAGG CACAAATGGA TATCGATGAA AGTGCAGATA TTATTATGAT AAAACCAGGG ATGCCATATC TAGATGTAAT TAAGTCCATT AGTAGCAGAT GTCAGATACC AGTATTTGCA TACCAAGTAA GTGGAGAATA TGCAATGATT AAAGCTGCAT CACAAAATCA CTTGCTAGAT TATGATTCTG TTATCTACGA ATCTCTATTA GGATTTAAGC GTGCAGGTGC AAAAAGTATA ATTACTTACG CTGCAATAGA TGCAGCAAAT ATACTCATAA AAAACAAAAT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA