EhchA.00935.a

cysteine desulfurase

CENTER ID: EhchA.00935.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00935.a.A1.GE27382 Full length( EhchA.00935.a ) 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00935.a.A1.PS00380 Full length( EhchA.00935.a ) 1 410

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.547
RefSeq: YP_507439.1
UniProt: Q2GGJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEQEKRQINL PVFLDYQSTT KTDDRVLEAM MPYFKQFSNP HSRSHSFGWK AESAVELARE RVASLINAEA KEVIFTSGAT ESNNLAIKGV ANFYKNKGNH IITVRTEHKC VLDSCRYLET EGFHVTYLDV QKNGILDLEL LKSSITDKTI LVSVMMVNNE IGVIQPIEKI GKICHEHGIF FHTDAAQAFG KISIDVKKMN IDLLSISGHK IYAPMGIGAL YIRKRQPRVR LTPMINGGGQ ERGMRSGTVP TPLAVGLGEA ARIAQEVMEE ENIRIRELRD ILYNEIKKHL PYVVLNGDYE QRIAGNLNLS FPYVEGESII MAINNLAVSS GSACTSASLE PSYVLRALNI EKDLEHSSIR FGIGRFTTRE EILYAAELIV SSIKKLREMS PLWEMVQEGV DLNNIKWDAH
NT Sequence
ATGGAACAAG AAAAACGACA AATCAATTTA CCTGTGTTTC TCGACTATCA ATCTACAACA AAAACAGATG ATAGGGTATT AGAAGCTATG ATGCCCTACT TTAAACAATT TTCTAATCCT CACTCACGCA GTCACTCCTT TGGCTGGAAA GCTGAATCAG CAGTTGAGTT AGCCAGGGAA AGAGTCGCAT CTTTAATAAA TGCCGAAGCT AAAGAAGTAA TATTCACTTC AGGTGCAACA GAATCAAATA ACTTAGCAAT TAAAGGAGTA GCAAACTTTT ATAAAAACAA AGGAAATCAT ATAATTACAG TACGTACAGA ACATAAATGC GTTTTAGATT CATGCCGTTA CTTAGAGACA GAAGGGTTTC ATGTTACTTA CTTAGACGTA CAAAAAAACG GTATCTTAGA TTTAGAGTTA TTAAAATCAT CTATCACTGA TAAAACAATA CTAGTATCAG TAATGATGGT GAACAATGAA ATTGGCGTTA TTCAACCAAT TGAAAAAATA GGAAAAATTT GTCATGAACA TGGAATATTT TTTCATACTG ATGCAGCTCA AGCTTTTGGA AAAATATCAA TAGATGTCAA AAAAATGAAC ATCGATCTTT TAAGTATATC AGGACACAAG ATATATGCTC CAATGGGAAT AGGAGCACTA TATATACGCA AACGCCAACC ACGAGTACGC CTTACTCCTA TGATTAACGG AGGTGGTCAA GAGCGTGGTA TGAGATCAGG AACAGTACCT ACTCCATTAG CTGTAGGGTT AGGAGAAGCA GCACGTATAG CTCAAGAAGT AATGGAGGAA GAAAACATCA GGATAAGAGA ATTGCGAGAC ATTTTATATA ATGAAATAAA AAAACACTTA CCATATGTCG TATTAAACGG GGATTACGAA CAACGTATAG CAGGAAATCT AAATTTAAGT TTTCCATATG TTGAAGGAGA ATCTATAATT ATGGCAATCA ATAATCTCGC AGTCAGTTCA GGTTCTGCTT GTACTTCTGC TTCTTTAGAA CCATCCTATG TTTTACGTGC TTTAAATATT GAAAAAGACT TAGAGCATTC ATCCATCAGA TTTGGTATAG GTAGGTTTAC TACAAGAGAA GAAATTTTAT ATGCCGCAGA GCTTATTGTT AGCAGCATAA AAAAATTACG TGAGATGAGT CCATTATGGG AAATGGTCCA AGAAGGTGTA GACCTTAATA ATATCAAATG GGATGCACAT
Details for EhchA.00935.a.A1.GE27382
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 48kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMEQEKRQ INLPVFLDYQ STTKTDDRVL EAMMPYFKQF SNPHSRSHSF GWKAESAVEL ARERVASLIN AEAKEVIFTS GATESNNLAI KGVANFYKNK GNHIITVRTE HKCVLDSCRY LETEGFHVTY LDVQKNGILD LELLKSSITD KTILVSVMMV NNEIGVIQPI EKIGKICHEH GIFFHTDAAQ AFGKISIDVK KMNIDLLSIS GHKIYAPMGI GALYIRKRQP RVRLTPMING GGQERGMRSG TVPTPLAVGL GEAARIAQEV MEEENIRIRE LRDILYNEIK KHLPYVVLNG DYEQRIAGNL NLSFPYVEGE SIIMAINNLA VSSGSACTSA SLEPSYVLRA LNIEKDLEHS SIRFGIGRFT TREEILYAAE LIVSSIKKLR EMSPLWEMVQ EGVDLNNIKW DAH
Validated NT Sequence
catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggaacaa gaaaaacgac aaatcaattt acctgtgttt ctcgactatc aatctacaac aaaaacagat gatagggtat tagaagctat gatgccctac tttaaacaat tttctaatcc tcactcacgc agtcactcct ttggctggaa agctgaatca gcagttgagt tagccaggga aagagtcgca tctttaataa atgccgaagc taaagaagta atattcactt caggtgcaac agaatcaaat aacttagcaa ttaaaggagt agcaaacttt tataaaaaca aaggaaatca tataattaca gtacgtacag aacataaatg cgttttagat tcatgccgtt acttagagac agaagggttt catgttactt acttagacgt acaaaaaaac ggtatcttag atttagagtt attaaaatca tctatcactg ataaaacaat actagtatca gtaatgatgg tgaacaatga aattggcgtt attcaaccaa ttgaaaaaat aggaaaaatt tgtcatgaac atggaatatt ttttcatact gatgcagctc aagcttttgg aaaaatatca atagatgtca aaaaaatgaa catcgatctt ttaagtatat caggacacaa gatatatgct ccaatgggaa taggagcact atatatacgc aaacgccaac cacgagtacg ccttactcct atgattaacg gaggtggtca agagcgtggt atgagatcag gaacagtacc tactccatta gctgtagggt taggagaagc agcacgtata gctcaagaag taatggagga agaaaacatc aggataagag aattgcgaga cattttatat aatgaaataa aaaaacactt accatatgtc gtattaaacg gggattacga acaacgtata gcaggaaatc taaatttaag ttttccatat gttgaaggag aatctataat tatggcaatc aataatctcg cagtcagttc aggttctgct tgtacttctg cttctttaga accatcctat gttttacgtg ctttaaatat tgaaaaagac ttagagcatt catccatcag atttggtata ggtaggttta ctacaagaga agaaatttta tatgccgcag agcttattgt tagcagcata aaaaaattac gtgagatgag tccattatgg gaaatggtcc aagaaggtgt agaccttaat aatatcaaat gggatgcaca ttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEQEKRQIN LPVFLDYQST TKTDDRVLEA MMPYFKQFSN PHSRSHSFGW KAESAVELAR ERVASLINAE AKEVIFTSGA TESNNLAIKG VANFYKNKGN HIITVRTEHK CVLDSCRYLE TEGFHVTYLD VQKNGILDLE LLKSSITDKT ILVSVMMVNN EIGVIQPIEK IGKICHEHGI FFHTDAAQAF GKISIDVKKM NIDLLSISGH KIYAPMGIGA LYIRKRQPRV RLTPMINGGG QERGMRSGTV PTPLAVGLGE AARIAQEVME EENIRIRELR DILYNEIKKH LPYVVLNGDY EQRIAGNLNL SFPYVEGESI IMAINNLAVS SGSACTSASL EPSYVLRALN IEKDLEHSSI RFGIGRFTTR EEILYAAELI VSSIKKLREM SPLWEMVQEG VDLNNIKWDA H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaacaagaa aaacgacaaa tcaatttacc tgtgtttctc gactatcaat ctacaacaaa aacagatgat agggtattag aagctatgat gccctacttt aaacaatttt ctaatcctca ctcacgcagt cactcctttg gctggaaagc tgaatcagca gttgagttag ccagggaaag agtcgcatct ttaataaatg ccgaagctaa agaagtaata ttcacttcag gtgcaacaga atcaaataac ttagcaatta aaggagtagc aaacttttat aaaaacaaag gaaatcatat aattacagta cgtacagaac ataaatgcgt tttagattca tgccgttact tagagacaga agggtttcat gttacttact tagacgtaca aaaaaacggt atcttagatt tagagttatt aaaatcatct atcactgata aaacaatact agtatcagta atgatggtga acaatgaaat tggcgttatt caaccaattg aaaaaatagg aaaaatttgt catgaacatg gaatattttt tcatactgat gcagctcaag cttttggaaa aatatcaata gatgtcaaaa aaatgaacat cgatctttta agtatatcag gacacaagat atatgctcca atgggaatag gagcactata tatacgcaaa cgccaaccac gagtacgcct tactcctatg attaacggag gtggtcaaga gcgtggtatg agatcaggaa cagtacctac tccattagct gtagggttag gagaagcagc acgtatagct caagaagtaa tggaggaaga aaacatcagg ataagagaat tgcgagacat tttatataat gaaataaaaa aacacttacc atatgtcgta ttaaacgggg attacgaaca acgtatagca ggaaatctaa atttaagttt tccatatgtt gaaggagaat ctataattat ggcaatcaat aatctcgcag tcagttcagg ttctgcttgt acttctgctt ctttagaacc atcctatgtt ttacgtgctt taaatattga aaaagactta gagcattcat ccatcagatt tggtataggt aggtttacta caagagaaga aattttatat gccgcagagc ttattgttag cagcataaaa aaattacgtg agatgagtcc attatgggaa atggtccaag aaggtgtaga ccttaataat atcaaatggg atgcacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EhchA.00935.a.A1.PS00380
PURIFICATION DATe: 10/21/2009
CONCENTRATION: 48.55mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMEQEKRQ INLPVFLDYQ STTKTDDRVL EAMMPYFKQF SNPHSRSHSF GWKAESAVEL ARERVASLIN AEAKEVIFTS GATESNNLAI KGVANFYKNK GNHIITVRTE HKCVLDSCRY LETEGFHVTY LDVQKNGILD LELLKSSITD KTILVSVMMV NNEIGVIQPI EKIGKICHEH GIFFHTDAAQ AFGKISIDVK KMNIDLLSIS GHKIYAPMGI GALYIRKRQP RVRLTPMING GGQERGMRSG TVPTPLAVGL GEAARIAQEV MEEENIRIRE LRDILYNEIK KHLPYVVLNG DYEQRIAGNL NLSFPYVEGE SIIMAINNLA VSSGSACTSA SLEPSYVLRA LNIEKDLEHS SIRFGIGRFT TREEILYAAE LIVSSIKKLR EMSPLWEMVQ EGVDLNNIKW DAH
Validated NT Sequence
catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggaacaa gaaaaacgac aaatcaattt acctgtgttt ctcgactatc aatctacaac aaaaacagat gatagggtat tagaagctat gatgccctac tttaaacaat tttctaatcc tcactcacgc agtcactcct ttggctggaa agctgaatca gcagttgagt tagccaggga aagagtcgca tctttaataa atgccgaagc taaagaagta atattcactt caggtgcaac agaatcaaat aacttagcaa ttaaaggagt agcaaacttt tataaaaaca aaggaaatca tataattaca gtacgtacag aacataaatg cgttttagat tcatgccgtt acttagagac agaagggttt catgttactt acttagacgt acaaaaaaac ggtatcttag atttagagtt attaaaatca tctatcactg ataaaacaat actagtatca gtaatgatgg tgaacaatga aattggcgtt attcaaccaa ttgaaaaaat aggaaaaatt tgtcatgaac atggaatatt ttttcatact gatgcagctc aagcttttgg aaaaatatca atagatgtca aaaaaatgaa catcgatctt ttaagtatat caggacacaa gatatatgct ccaatgggaa taggagcact atatatacgc aaacgccaac cacgagtacg ccttactcct atgattaacg gaggtggtca agagcgtggt atgagatcag gaacagtacc tactccatta gctgtagggt taggagaagc agcacgtata gctcaagaag taatggagga agaaaacatc aggataagag aattgcgaga cattttatat aatgaaataa aaaaacactt accatatgtc gtattaaacg gggattacga acaacgtata gcaggaaatc taaatttaag ttttccatat gttgaaggag aatctataat tatggcaatc aataatctcg cagtcagttc aggttctgct tgtacttctg cttctttaga accatcctat gttttacgtg ctttaaatat tgaaaaagac ttagagcatt catccatcag atttggtata ggtaggttta ctacaagaga agaaatttta tatgccgcag agcttattgt tagcagcata aaaaaattac gtgagatgag tccattatgg gaaatggtcc aagaaggtgt agaccttaat aatatcaaat gggatgcaca ttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEQEKRQIN LPVFLDYQST TKTDDRVLEA MMPYFKQFSN PHSRSHSFGW KAESAVELAR ERVASLINAE AKEVIFTSGA TESNNLAIKG VANFYKNKGN HIITVRTEHK CVLDSCRYLE TEGFHVTYLD VQKNGILDLE LLKSSITDKT ILVSVMMVNN EIGVIQPIEK IGKICHEHGI FFHTDAAQAF GKISIDVKKM NIDLLSISGH KIYAPMGIGA LYIRKRQPRV RLTPMINGGG QERGMRSGTV PTPLAVGLGE AARIAQEVME EENIRIRELR DILYNEIKKH LPYVVLNGDY EQRIAGNLNL SFPYVEGESI IMAINNLAVS SGSACTSASL EPSYVLRALN IEKDLEHSSI RFGIGRFTTR EEILYAAELI VSSIKKLREM SPLWEMVQEG VDLNNIKWDA H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAACAAGAA AAACGACAAA TCAATTTACC TGTGTTTCTC GACTATCAAT CTACAACAAA AACAGATGAT AGGGTATTAG AAGCTATGAT GCCCTACTTT AAACAATTTT CTAATCCTCA CTCACGCAGT CACTCCTTTG GCTGGAAAGC TGAATCAGCA GTTGAGTTAG CCAGGGAAAG AGTCGCATCT TTAATAAATG CCGAAGCTAA AGAAGTAATA TTCACTTCAG GTGCAACAGA ATCAAATAAC TTAGCAATTA AAGGAGTAGC AAACTTTTAT AAAAACAAAG GAAATCATAT AATTACAGTA CGTACAGAAC ATAAATGCGT TTTAGATTCA TGCCGTTACT TAGAGACAGA AGGGTTTCAT GTTACTTACT TAGACGTACA AAAAAACGGT ATCTTAGATT TAGAGTTATT AAAATCATCT ATCACTGATA AAACAATACT AGTATCAGTA ATGATGGTGA ACAATGAAAT TGGCGTTATT CAACCAATTG AAAAAATAGG AAAAATTTGT CATGAACATG GAATATTTTT TCATACTGAT GCAGCTCAAG CTTTTGGAAA AATATCAATA GATGTCAAAA AAATGAACAT CGATCTTTTA AGTATATCAG GACACAAGAT ATATGCTCCA ATGGGAATAG GAGCACTATA TATACGCAAA CGCCAACCAC GAGTACGCCT TACTCCTATG ATTAACGGAG GTGGTCAAGA GCGTGGTATG AGATCAGGAA CAGTACCTAC TCCATTAGCT GTAGGGTTAG GAGAAGCAGC ACGTATAGCT CAAGAAGTAA TGGAGGAAGA AAACATCAGG ATAAGAGAAT TGCGAGACAT TTTATATAAT GAAATAAAAA AACACTTACC ATATGTCGTA TTAAACGGGG ATTACGAACA ACGTATAGCA GGAAATCTAA ATTTAAGTTT TCCATATGTT GAAGGAGAAT CTATAATTAT GGCAATCAAT AATCTCGCAG TCAGTTCAGG TTCTGCTTGT ACTTCTGCTT CTTTAGAACC ATCCTATGTT TTACGTGCTT TAAATATTGA AAAAGACTTA GAGCATTCAT CCATCAGATT TGGTATAGGT AGGTTTACTA CAAGAGAAGA AATTTTATAT GCCGCAGAGC TTATTGTTAG CAGCATAAAA AAATTACGTG AGATGAGTCC ATTATGGGAA ATGGTCCAAG AAGGTGTAGA CCTTAATAAT ATCAAATGGG ATGCACATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA