EhchA.00925.a

fructose-bisphosphate aldolase

CENTER ID: EhchA.00925.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00925.a.A1.GE27387 Full length( EhchA.00925.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.82
RefSeq: YP_506925.2
UniProt: Q2GI08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLANNRIQDI LGFYESENPG VKANLMRMLL HGKVAGSGKL LILPVDQGME HGPTKSFSKN PQSYDPHYHF LLAIEGGFSA YAAPLGMLEA GASTYAGLIP LILKLNSSTL LSPKNSFPDQ VVTSSVKDAL RLGCSAIGIT IYPGSHNFFS MVRETRELIA EAKAAGLAVV IWSYPRGNDI SKQGETAVDI VAYAAHIAAS LGANIIKVKL PTSNIERDSI PYDITSLKQR IEYIKLSCFS GRRLVVFSGG EAKTDEELLQ EIQSIKLGGG DGSIIGRNSF QRPKNDAISM VGKIAKIYHN
NT Sequence
GTGCTAGCCA ACAACCGTAT TCAGGACATT CTAGGATTCT ATGAAAGTGA GAACCCAGGA GTAAAAGCAA ATTTGATGAG AATGCTGTTA CACGGTAAAG TAGCTGGCAG TGGGAAGTTG TTAATATTAC CTGTAGACCA AGGAATGGAG CATGGACCAA CTAAAAGTTT TTCAAAGAAT CCTCAGTCTT ATGACCCACA CTATCATTTT CTATTAGCTA TTGAAGGAGG GTTTAGTGCA TACGCAGCTC CACTTGGTAT GCTTGAAGCT GGAGCATCTA CTTACGCTGG TCTGATACCT CTTATACTAA AACTAAATAG CTCTACACTG CTTTCCCCAA AGAACAGTTT CCCTGATCAA GTGGTAACTT CTTCTGTAAA AGATGCACTA AGGTTAGGAT GTTCAGCTAT AGGAATCACT ATTTATCCAG GTTCCCACAA TTTTTTCTCT ATGGTTCGAG AAACAAGAGA GCTAATTGCA GAAGCAAAAG CAGCAGGTTT AGCAGTTGTA ATATGGTCAT ACCCACGTGG AAATGATATT TCAAAACAAG GAGAAACAGC AGTAGACATA GTAGCTTATG CTGCACACAT TGCAGCATCG CTTGGAGCAA ACATTATAAA GGTTAAATTA CCAACAAGTA ATATAGAAAG AGATTCTATA CCTTATGATA TTACATCTTT AAAACAAAGG ATAGAGTATA TAAAATTATC TTGCTTTTCA GGAAGAAGAT TGGTTGTTTT TTCAGGTGGA GAAGCAAAAA CCGACGAAGA ATTATTGCAA GAAATACAAT CAATAAAATT AGGTGGAGGA GATGGATCAA TTATAGGTCG TAATTCCTTT CAAAGACCAA AAAATGATGC CATATCAATG GTAGGAAAAA TAGCAAAAAT TTATCATAAC
Details for EhchA.00925.a.A1.GE27387
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 72
Validated AA Sequence
MTXTIXXYXL XKXGFSAYAA PXGMLEAGAX TYAGLIPLIL KLNSSTLLSP KNSFPDQVVT SSVKDALRLG CSAIGITIYP GSHNFFSMVR ETRELIAEAK AAGLAVVIWS YPRGNDISKQ GETAVDIVAY AAHIAASLGA NIIKVKLPTS NIERDSIPYD ITSLKQRIEY IKLSCFSGRR LVVFSGGEAK TDEELLQEIQ SIKLGGGDGS IIGRNSFQRP KNDAISMVGK IAKI
Validated NT Sequence
ataaattttt gctatttttc ctaccattga tatggcatca ttttttggtc tttgaaagga attacgacct ataattgatc catctcctcc acctaatttt attgattgta tttcttgcaa taattcttcg tcggtttttg cttctccacc tgaaaaaaca accaatcttc ttcctgaaaa gcaagataat tttatatact ctatcctttg ttttaaagat gtaatatcat aaggtataga atctctttct atattacttg ttggtaattt aacctttata atgtttgctc caagcgatgc tgcaatgtgt gcagcataag ctactatgtc tactgctgtt tctccttgtt ttgaaatatc atttccacgt gggtatgacc atattacaac tgctaaacct gctgcttttg cttctgcaat tagctctctt gtttctcgaa ccatagagaa aaaattgtgg gaacctggat aaatagtgat tcctatagct gaacatccta accttagtgc atcttttaca gaagaagtta ccacttgatc agggaaactg ttctttgggg aaagcagtgt agagctattt agttttagta taagaggtat cagaccagcg taagtanatg ctccagcttc nagcataccn nntggagctg cgtatgcact aaacccntnc ttcnatagct natannaaan gatagtgnng gtcataagac tgaggnntct ttgaaaaact ttnagttggt cnntgctcca ttcnntggtc tannngnnat antaacaacn tcccactgnc agctactttn ncgngtaaca gcnntctcat caaatttgct tttactcnng ggttctcact ttcatagaat cntagaatgt cnngaatacg gttgtnggnn agcaccatng aaccaggacc cngggtctga gcttnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLANNRIQ DILGFYESEN PGVKANLMRM LLHGKVAGSG KLLILPVDQG MEHGPTKSFS KNPQSYDPHY HFLLAIEGGF SAYAAPLGML EAGASTYAGL IPLILKLNSS TLLSPKNSFP DQVVTSSVKD ALRLGCSAIG ITIYPGSHNF FSMVRETREL IAEAKAAGLA VVIWSYPRGN DISKQGETAV DIVAYAAHIA ASLGANIIKV KLPTSNIERD SIPYDITSLK QRIEYIKLSC FSGRRLVVFS GGEAKTDEEL LQEIQSIKLG GGDGSIIGRN SFQRPKNDAI SMVGKIAKIY HN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctagcc aacaaccgta ttcaggacat tctaggattc tatgaaagtg agaacccagg agtaaaagca aatttgatga gaatgctgtt acacggtaaa gtagctggca gtgggaagtt gttaatatta cctgtagacc aaggaatgga gcatggacca actaaaagtt tttcaaagaa tcctcagtct tatgacccac actatcattt tctattagct attgaaggag ggtttagtgc atacgcagct ccacttggta tgcttgaagc tggagcatct acttacgctg gtctgatacc tcttatacta aaactaaata gctctacact gctttcccca aagaacagtt tccctgatca agtggtaact tcttctgtaa aagatgcact aaggttagga tgttcagcta taggaatcac tatttatcca ggttcccaca attttttctc tatggttcga gaaacaagag agctaattgc agaagcaaaa gcagcaggtt tagcagttgt aatatggtca tacccacgtg gaaatgatat ttcaaaacaa ggagaaacag cagtagacat agtagcttat gctgcacaca ttgcagcatc gcttggagca aacattataa aggttaaatt accaacaagt aatatagaaa gagattctat accttatgat attacatctt taaaacaaag gatagagtat ataaaattat cttgcttttc aggaagaaga ttggttgttt tttcaggtgg agaagcaaaa accgacgaag aattattgca agaaatacaa tcaataaaat taggtggagg agatggatca attataggtc gtaattcctt tcaaagacca aaaaatgatg ccatatcaat ggtaggaaaa atagcaaaaa tttatcataa caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa