EhchA.00908.a

adenylosuccinate synthetase

CENTER ID: EhchA.00908.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00908.a.A1.GE26783 Full length( EhchA.00908.a ) 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.394
RefSeq: YP_507278.1
UniProt: Q2GH05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNIVVVGLQ WGDEGKGKVV DWLSTNADAV VRFQGGNNAG HTIVINDKVY KLNLLPSSVL QNNKLSIIGN GVVLDPYALV SEIDNLKSSG INITTQNLAI SESCPLVLSV HKQADILFEQ LRQDTIGTTN KGIGPCYADK ISRRAIRVCD LLDTKDLLYS KVNHLLIYHN LLRKSLNTPP IKAEDIVNEL LNIAPKILPF VQPVWKTIYN LTQQNKTIIF EGAQGTFLDI DHGTYPFVTS SNTIASQAWV GCGINPSNKS YILGLVKAYT TRVGNGPFFT EQNNDIGKIM FKTGKELGTV SNRKRRCGWF DAVLARQAIM LSGVSGLVMT KLDVLDQFSE IKICVKYKYG DKTYDYLPAS PHIQSNLEPI YEILPGWQTS TFGSVSYKDL PQNAILYIKK IEEILKVPIH LISTGPERNS MIILNNDFLQ
NT Sequence
ATGGTAAATA TAGTAGTTGT AGGGTTACAA TGGGGTGATG AAGGAAAAGG AAAAGTCGTA GATTGGCTAT CCACTAATGC AGACGCTGTC GTGAGATTTC AAGGAGGCAA TAATGCAGGA CATACTATAG TCATAAATGA TAAAGTATAT AAGTTAAACC TTCTTCCATC TTCTGTTTTA CAAAACAATA AACTGTCCAT AATAGGAAAT GGAGTAGTTT TGGACCCTTA TGCACTTGTT TCAGAAATTG ATAACTTGAA AAGCAGTGGT ATTAACATTA CTACTCAAAA TCTTGCAATA TCAGAATCTT GTCCACTAGT GCTTAGTGTA CACAAACAAG CAGATATACT GTTTGAACAA CTTAGACAAG ATACTATAGG AACCACAAAC AAAGGAATTG GCCCATGTTA TGCAGATAAA ATCAGCAGAA GAGCTATCAG AGTATGTGAT TTACTAGATA CAAAAGATCT GCTATACAGC AAAGTTAATC ATCTTCTAAT CTACCATAAT CTATTAAGAA AAAGTCTTAA TACCCCACCT ATAAAGGCAG AGGATATAGT TAATGAACTG TTGAATATTG CCCCAAAAAT TTTACCATTC GTACAACCAG TATGGAAAAC CATATATAAT TTAACACAAC AAAATAAGAC AATAATTTTT GAGGGAGCAC AAGGAACATT CCTAGATATT GACCATGGAA CATATCCTTT TGTTACATCA AGTAATACCA TAGCATCACA AGCATGGGTC GGATGTGGTA TCAATCCATC TAATAAGTCT TACATACTGG GATTAGTAAA AGCCTATACA ACAAGAGTAG GTAATGGTCC ATTTTTTACT GAACAAAACA ATGATATAGG AAAAATCATG TTTAAAACAG GAAAAGAATT AGGAACTGTC AGTAATAGGA AAAGAAGATG TGGATGGTTT GATGCAGTAC TAGCACGACA AGCAATAATG CTATCTGGAG TATCAGGATT AGTTATGACT AAGCTCGATG TACTAGATCA ATTCAGTGAA ATAAAGATAT GTGTAAAATA TAAATACGGA GATAAAACAT ACGATTACCT CCCTGCTTCA CCTCACATAC AAAGTAATTT AGAACCAATA TATGAAATAC TTCCTGGATG GCAAACAAGC ACATTTGGCA GTGTATCGTA TAAGGATTTA CCACAAAACG CTATATTGTA CATAAAGAAA ATAGAAGAAA TCCTAAAAGT CCCTATTCAT TTGATTTCTA CAGGCCCAGA AAGAAATTCT ATGATTATAC TCAACAATGA TTTTCTACAA
Details for EhchA.00908.a.A1.GE26783
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 94
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNIVVVGL QWGDEGKGKV VDWPSTNADA VVRFQGGNNA GHTIVINDKV YKLNLLPSPV LQNNKLSIIG NGVVLDPYAL VSEIDNLKSS GINITTQNLA ISESCPLVLS VHXQADILFE QLRQDXIGTT NKGIGPCXAD KISRRAIRVC DLXDTKDLLY SKVXXLXIYX NXLRKSLXTP XIKAXDXVNE LLNIXXXXXX XXQPXWXXIY NXTQQSKTII XEGAQGTFLD IDHGTYPFVT SSNTIASQAW VGCGINPSNK SYILGLVKAY TTRVGNGPFF TEQNNDIGKI MFKTGKELGT VSNRKRRCGW FDAVLARQAI MLSGVSGLVM TKLDVLDQFS EIKICVKYKY GDKTYDYLPA SPHIQSNLEL IYEILPGWQT STFGSVSYKD LPQNAILYIK KIEEILKVPI HLISTGPERN SMIILNNDFL Q
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNIVVVGL QWGDEGKGKV VDWLSTNADA VVRFQGGNNA GHTIVINDKV YKLNLLPSSV LQNNKLSIIG NGVVLDPYAL VSEIDNLKSS GINITTQNLA ISESCPLVLS VHKQADILFE QLRQDTIGTT NKGIGPCYAD KISRRAIRVC DLLDTKDLLY SKVNHLLIYH NLLRKSLNTP PIKAEDIVNE LLNIAPKILP FVQPVWKTIY NLTQQNKTII FEGAQGTFLD IDHGTYPFVT SSNTIASQAW VGCGINPSNK SYILGLVKAY TTRVGNGPFF TEQNNDIGKI MFKTGKELGT VSNRKRRCGW FDAVLARQAI MLSGVSGLVM TKLDVLDQFS EIKICVKYKY GDKTYDYLPA SPHIQSNLEP IYEILPGWQT STFGSVSYKD LPQNAILYIK KIEEILKVPI HLISTGPERN SMIILNNDFL Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaaatata gtagttgtag ggttacaatg gggtgatgaa ggaaaaggaa aagtcgtaga ttggctatcc actaatgcag acgctgtcgt gagatttcaa ggaggcaata atgcaggaca tactatagtc ataaatgata aagtatataa gttaaacctt cttccatctt ctgttttaca aaacaataaa ctgtccataa taggaaatgg agtagttttg gacccttatg cacttgtttc agaaattgat aacttgaaaa gcagtggtat taacattact actcaaaatc ttgcaatatc agaatcttgt ccactagtgc ttagtgtaca caaacaagca gatatactgt ttgaacaact tagacaagat actataggaa ccacaaacaa aggaattggc ccatgttatg cagataaaat cagcagaaga gctatcagag tatgtgattt actagataca aaagatctgc tatacagcaa agttaatcat cttctaatct accataatct attaagaaaa agtcttaata ccccacctat aaaggcagag gatatagtta atgaactgtt gaatattgcc ccaaaaattt taccattcgt acaaccagta tggaaaacca tatataattt aacacaacaa aataagacaa taatttttga gggagcacaa ggaacattcc tagatattga ccatggaaca tatccttttg ttacatcaag taataccata gcatcacaag catgggtcgg atgtggtatc aatccatcta ataagtctta catactggga ttagtaaaag cctatacaac aagagtaggt aatggtccat tttttactga acaaaacaat gatataggaa aaatcatgtt taaaacagga aaagaattag gaactgtcag taataggaaa agaagatgtg gatggtttga tgcagtacta gcacgacaag caataatgct atctggagta tcaggattag ttatgactaa gctcgatgta ctagatcaat tcagtgaaat aaagatatgt gtaaaatata aatacggaga taaaacatac gattacctcc ctgcttcacc tcacatacaa agtaatttag aaccaatata tgaaatactt cctggatggc aaacaagcac atttggcagt gtatcgtata aggatttacc acaaaacgct atattgtaca taaagaaaat agaagaaatc ctaaaagtcc ctattcattt gatttctaca ggcccagaaa gaaattctat gattatactc aacaatgatt ttctacaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa