SSGCID: Target Page

EhchA.00899.a

guanylate kinase

CENTER ID:	EhchA.00899.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00899.a.A1.GE26766	Full length( EhchA.00899.a )		1	209

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.275
RefSeq:	YP_507143.1
UniProt:	Q2GHE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDSLKSRGI MLVISSPSGG GKTTISHLLI NELQNDLVRS ISVTTREPRD GEINGKDYFF VTEPEFINLC NTNQMLEYAK VFGNYYGIPR KFVTDNIANG VSVLFSIDWQ GAFKLIDIMR EHVVSIFILP PSMEELQRRL YNRSGESDVI NKRLGEAAFE ISHCYRYDYV IVNHDIEQSV YQIKCIFTSE KLKTQRRVSL KQFIDNCIR

NT Sequence

GTGAATGATA GTTTAAAAAG TAGGGGAATA ATGTTAGTTA TATCATCTCC TTCTGGAGGT GGTAAAACTA CAATATCTCA TTTGTTAATT AATGAATTAC AAAATGATCT TGTTAGGTCA ATTTCTGTTA CTACTCGTGA ACCTAGAGAT GGTGAAATCA ATGGTAAAGA TTATTTTTTT GTAACTGAAC CTGAGTTTAT CAATTTGTGT AATACAAATC AGATGCTAGA GTATGCAAAA GTTTTTGGGA ATTACTATGG TATACCCCGT AAGTTTGTTA CCGATAATAT TGCTAATGGT GTTAGTGTAT TGTTTAGTAT TGATTGGCAA GGGGCGTTTA AATTAATTGA TATTATGCGA GAACATGTGG TGAGTATATT CATATTACCT CCATCTATGG AAGAGTTGCA AAGGCGACTA TATAATCGTA GTGGAGAAAG CGATGTAATA AATAAAAGAT TAGGAGAAGC TGCTTTTGAA ATTAGTCATT GTTATCGTTA TGACTATGTT ATTGTTAACC ATGATATTGA ACAAAGTGTT TATCAGATTA AATGTATTTT TACTTCAGAA AAATTGAAAA CGCAACGTAG GGTATCTCTA AAACAATTTA TTGATAATTG TATCAGA

Details for EhchA.00899.a.A1.GE26766

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/1/2009
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNDSLKSR GIMLVISSPS GGGKTTISHL LINELQNDLV RSISVTTREP RDGEINGKDY FFVTEPEFIN LCNTNQMLEY AKVFGNYYGI PRKFVTDNIA NGVGVLFSID WQGAFKLIDI MREHVVSIFI LPPSMEELQR RLYNRSGESD VINKRLGEAA FEISHCYRYD YVIVNHDIEQ SVYQIKCIFT SEKLKTQRRV SLKQFIDNCI R

Validated NT Sequence

ttattatctg atacaattat caataaattg ttttagagat accctacgtt gcgttttcaa tttttctgaa gtaaaaatac atttaatctg ataaacactt tgttcaatat catggttaac aataacatag tcataacgat aacaatgact aatttcaaaa gcagcttctc ctaatctttt atttattaca tcgctttctc cactacgatt atatagtcgc ctttgcaact cttccataga tggaggtaat atgaatatac tcaccacatg ttctcgcata atatcaatta atttaaacgc cccttgccaa tcaatactaa acaatacacc aacaccatta gcaatattat cggtaacaaa cttacggggt ataccatagt aattcccaaa aacttttgca tactctagca tctgatttgt attacacaaa ttgataaact caggttcagt tacaaaaaaa taatctttac cattgatttc accatctcta ggttcacgag tagtaacaga aattgaccta acaagatcat tttgtaattc attaattaac aaatgagata ttgtagtttt accacctcca gaaggagatg atataactag cattattccc ctacttttta aactatcatt caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNDSLKSR GIMLVISSPS GGGKTTISHL LINELQNDLV RSISVTTREP RDGEINGKDY FFVTEPEFIN LCNTNQMLEY AKVFGNYYGI PRKFVTDNIA NGVSVLFSID WQGAFKLIDI MREHVVSIFI LPPSMEELQR RLYNRSGESD VINKRLGEAA FEISHCYRYD YVIVNHDIEQ SVYQIKCIFT SEKLKTQRRV SLKQFIDNCI R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatgat agtttaaaaa gtaggggaat aatgttagtt atatcatctc cttctggagg tggtaaaact acaatatctc atttgttaat taatgaatta caaaatgatc ttgttaggtc aatttctgtt actactcgtg aacctagaga tggtgaaatc aatggtaaag attatttttt tgtaactgaa cctgagttta tcaatttgtg taatacaaat cagatgctag agtatgcaaa agtttttggg aattactatg gtataccccg taagtttgtt accgataata ttgctaatgg tgttagtgta ttgtttagta ttgattggca aggggcgttt aaattaattg atattatgcg agaacatgtg gtgagtatat tcatattacc tccatctatg gaagagttgc aaaggcgact atataatcgt agtggagaaa gcgatgtaat aaataaaaga ttaggagaag ctgcttttga aattagtcat tgttatcgtt atgactatgt tattgttaac catgatattg aacaaagtgt ttatcagatt aaatgtattt ttacttcaga aaaattgaaa acgcaacgta gggtatctct aaaacaattt attgataatt gtatcagaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa