EhchA.00865.a
phosphoribosylamine--glycine ligase
| CENTER ID: | EhchA.00865.a |
| ORGANISM: | Ehrlichia chaffeensis str. Arkansas |
| ASSOCIATED DISEASE: | Human monocytic ehrlichiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.00865.a.A1.GE26765 | Full length( EhchA.00865.a ) | 1 | 421 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.00865.a.A1.PW27470 | Full length( EhchA.00865.a ) | 1 | 421 |
Structures
3LP8
DEPOSITED: 2/4/2010
DETERMINATION: XRay
CLONE: EhchA.00865.a.A1.GE26765
PROTEIN: EhchA.00865.a.A1.PW27470
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126894 |
| PATRIC ID: | fig|205920.11.peg.867 |
| RefSeq: | YP_507793.1 |
| UniProt: | Q2GFJ0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVLVIGSGG REHSMLHHIR KSTLLNKLFI APGREGMSGL ADIIDIDINS TIEVIQVCKK EKIELVVIGP ETPLMNGLSD ALTEEGILVF GPSKAAARLE SSKGFTKELC MRYGIPTAKY GYFVDTNSAY KFIDKHKLPL VVKADGLAQG KGTVICHTHE EAYNAVDAML VHHKFGEAGC AIIIEEFLEG KEISFFTLVD GSNPVILGVA QDYKTIGDNN KGPNTGGMGS YSKPNIITQE MEHIIIQKII YPTIKAMFNM NIQFRGLLFA GIIIKKNEPK LLEYNVRFGD PETQSILPRL NSDFLKLLSL TAKGKLGNES VELSKKAALC VVVASRGYPG EYKKNSIING IENIEKLPNV QLLHAGTRRE GNNWVSDSGR VINVVAQGEN LASAKHQAYA ALDLLDWPDG IYRYDIGSCA L
NT Sequence
ATGAATGTAT TGGTGATTGG TTCTGGCGGC CGTGAGCACT CAATGTTGCA TCACATTCGT AAATCTACAT TACTAAACAA GCTATTTATC GCCCCAGGGC GTGAAGGAAT GTCTGGGTTA GCAGATATAA TAGATATAGA TATCAATAGC ACAATAGAAG TAATTCAAGT ATGTAAGAAA GAAAAAATTG AATTAGTAGT CATCGGACCA GAAACTCCAT TAATGAATGG ATTATCAGAC GCATTAACAG AAGAAGGCAT ATTAGTCTTT GGACCTTCTA AAGCAGCAGC GCGTCTTGAA TCTTCAAAAG GATTTACAAA AGAATTATGC ATGAGGTATG GAATTCCTAC TGCAAAATAC GGGTACTTTG TTGATACAAA TTCAGCTTAC AAATTCATTG ATAAACACAA ATTACCTTTA GTAGTTAAGG CTGATGGGTT AGCCCAGGGA AAAGGAACAG TGATATGTCA CACACACGAA GAAGCATACA ATGCTGTAGA TGCCATGTTA GTGCACCACA AATTTGGAGA AGCTGGTTGT GCAATAATTA TTGAAGAATT CCTTGAAGGC AAGGAAATTA GCTTCTTTAC ATTGGTTGAC GGATCCAACC CAGTTATACT TGGCGTAGCA CAAGATTATA AAACTATAGG AGATAATAAT AAAGGTCCTA ATACTGGAGG GATGGGATCA TACTCTAAAC CAAATATCAT TACACAAGAA ATGGAGCATA TAATAATTCA GAAGATAATA TATCCAACTA TTAAAGCAAT GTTCAACATG AATATACAGT TTAGAGGTCT GTTATTCGCT GGTATTATAA TCAAAAAAAA TGAACCAAAA TTACTTGAAT ATAATGTACG GTTTGGAGAT CCTGAAACAC AATCAATATT ACCAAGATTA AATTCCGATT TCTTAAAACT TTTATCACTA ACAGCTAAAG GTAAACTAGG AAATGAATCA GTAGAATTAA GTAAAAAAGC TGCTTTATGT GTTGTGGTAG CTAGTCGTGG ATATCCAGGT GAGTATAAGA AAAATTCTAT AATTAATGGA ATAGAAAATA TTGAAAAGCT ACCTAATGTT CAGCTCTTAC ATGCAGGCAC AAGAAGAGAA GGAAATAACT GGGTATCAGA TTCTGGAAGA GTAATAAATG TTGTAGCACA AGGTGAAAAT TTAGCTAGTG CGAAACACCA AGCCTACGCT GCATTGGACT TATTAGATTG GCCAGATGGA ATTTACAGAT ATGATATAGG ATCATGTGCT CTT
Details for EhchA.00865.a.A1.GE26765
| HARVESTED ON: | 6/18/2009 |
| SEQUENCED ON: | 7/1/2009 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNVLVIGSG GREHSMLHHI RKSTLLNKLF IAPGREGMSG LADIIDIDIN STIEVIQVCK KEKIELVVIG PETPLMNGLS DALTEEGILV FGPSKAAARL ESSKGFTKEL CMRYGIPTAK YGYFVDTNSA YKFIDKHKLP LVVKADGLAQ GKGTVICHTH EKAYNAVDAM LVHHKFGEAG CAIIIEEFLE GKEISFFTLV DGSNPVILGV AQDYKTIGDN NKGPNTGGMG SYSKPNIITQ EMEHIIIQKI IYPTIKAMFN MNIQFRGLLF AGIIIKKNEP KLLEYNVRFG DPETQSILPR LNSDFLKLLS LTAKGKLGNE SVELSKKAAL CVVVASRGYP GEYKKNSIIN GIENIEKLPN VQLLHAGTRR EGNNWVSDSG RVINVVAQGE NLASAKHQAY AALDLLDWPD GIYRYDIGSC AL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatg tattggtgat tggttctggc ggccgtgagc actcaatgtt gcatcacatt cgtaaatcta cattactaaa caagctattt atcgccccag ggcgtgaagg aatgtctggg ttagcagata taatagatat agatatcaat agcacaatag aagtaattca agtatgtaag aaagaaaaaa ttgaattagt agtcatcgga ccagaaactc cattaatgaa tggattatca gacgcattaa cagaagaagg catattagtc tttggacctt ctaaagcagc agcgcgtctt gaatcttcaa aaggatttac aaaagaatta tgcatgaggt atggaattcc tactgcaaaa tacgggtact ttgttgatac aaattcagct tacaaattca ttgataaaca caaattacct ttagtagtta aggctgatgg gttagcccag ggaaaaggaa cagtgatatg tcacacacac gaaaaagcat acaatgctgt agatgccatg ttagtgcacc acaaatttgg agaagctggt tgtgcaataa ttattgaaga attccttgaa ggcaaggaaa ttagcttctt tacattggtt gacggatcca acccagttat acttggcgta gcacaagatt ataaaactat aggagataat aataaaggtc ctaatactgg agggatggga tcatactcta aaccaaatat cattacacaa gaaatggagc atataataat tcagaagata atatatccaa ctattaaagc aatgttcaac atgaatatac agtttagagg tctgttattc gctggtatta taatcaaaaa aaatgaacca aaattacttg aatataatgt acggtttgga gatcctgaaa cacaatcaat attaccaaga ttaaattccg atttcttaaa acttttatca ctaacagcta aaggtaaact aggaaatgaa tcagtagaat taagtaaaaa agctgcttta tgtgttgtgg tagctagtcg tggatatcca ggtgagtata agaaaaattc tataattaat ggaatagaaa atattgaaaa gctacctaat gttcagctct tacatgcagg cacaagaaga gaaggaaata actgggtatc agattctgga agagtaataa atgttgtagc acaaggtgaa aatttagcta gtgcgaaaca ccaagcctac gctgcattgg acttattaga ttggccagat ggaatttaca gatatgatat aggatcatgt gctctttaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNVLVIGSG GREHSMLHHI RKSTLLNKLF IAPGREGMSG LADIIDIDIN STIEVIQVCK KEKIELVVIG PETPLMNGLS DALTEEGILV FGPSKAAARL ESSKGFTKEL CMRYGIPTAK YGYFVDTNSA YKFIDKHKLP LVVKADGLAQ GKGTVICHTH EEAYNAVDAM LVHHKFGEAG CAIIIEEFLE GKEISFFTLV DGSNPVILGV AQDYKTIGDN NKGPNTGGMG SYSKPNIITQ EMEHIIIQKI IYPTIKAMFN MNIQFRGLLF AGIIIKKNEP KLLEYNVRFG DPETQSILPR LNSDFLKLLS LTAKGKLGNE SVELSKKAAL CVVVASRGYP GEYKKNSIIN GIENIEKLPN VQLLHAGTRR EGNNWVSDSG RVINVVAQGE NLASAKHQAY AALDLLDWPD GIYRYDIGSC AL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgtattg gtgattggtt ctggcggccg tgagcactca atgttgcatc acattcgtaa atctacatta ctaaacaagc tatttatcgc cccagggcgt gaaggaatgt ctgggttagc agatataata gatatagata tcaatagcac aatagaagta attcaagtat gtaagaaaga aaaaattgaa ttagtagtca tcggaccaga aactccatta atgaatggat tatcagacgc attaacagaa gaaggcatat tagtctttgg accttctaaa gcagcagcgc gtcttgaatc ttcaaaagga tttacaaaag aattatgcat gaggtatgga attcctactg caaaatacgg gtactttgtt gatacaaatt cagcttacaa attcattgat aaacacaaat tacctttagt agttaaggct gatgggttag cccagggaaa aggaacagtg atatgtcaca cacacgaaga agcatacaat gctgtagatg ccatgttagt gcaccacaaa tttggagaag ctggttgtgc aataattatt gaagaattcc ttgaaggcaa ggaaattagc ttctttacat tggttgacgg atccaaccca gttatacttg gcgtagcaca agattataaa actataggag ataataataa aggtcctaat actggaggga tgggatcata ctctaaacca aatatcatta cacaagaaat ggagcatata ataattcaga agataatata tccaactatt aaagcaatgt tcaacatgaa tatacagttt agaggtctgt tattcgctgg tattataatc aaaaaaaatg aaccaaaatt acttgaatat aatgtacggt ttggagatcc tgaaacacaa tcaatattac caagattaaa ttccgatttc ttaaaacttt tatcactaac agctaaaggt aaactaggaa atgaatcagt agaattaagt aaaaaagctg ctttatgtgt tgtggtagct agtcgtggat atccaggtga gtataagaaa aattctataa ttaatggaat agaaaatatt gaaaagctac ctaatgttca gctcttacat gcaggcacaa gaagagaagg aaataactgg gtatcagatt ctggaagagt aataaatgtt gtagcacaag gtgaaaattt agctagtgcg aaacaccaag cctacgctgc attggactta ttagattggc cagatggaat ttacagatat gatataggat catgtgctct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.00865.a.A1.PW27470
| PURIFICATION DATe: | 8/11/2009 |
| CONCENTRATION: | 22.53mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNVLVIGSG GREHSMLHHI RKSTLLNKLF IAPGREGMSG LADIIDIDIN STIEVIQVCK KEKIELVVIG PETPLMNGLS DALTEEGILV FGPSKAAARL ESSKGFTKEL CMRYGIPTAK YGYFVDTNSA YKFIDKHKLP LVVKADGLAQ GKGTVICHTH EKAYNAVDAM LVHHKFGEAG CAIIIEEFLE GKEISFFTLV DGSNPVILGV AQDYKTIGDN NKGPNTGGMG SYSKPNIITQ EMEHIIIQKI IYPTIKAMFN MNIQFRGLLF AGIIIKKNEP KLLEYNVRFG DPETQSILPR LNSDFLKLLS LTAKGKLGNE SVELSKKAAL CVVVASRGYP GEYKKNSIIN GIENIEKLPN VQLLHAGTRR EGNNWVSDSG RVINVVAQGE NLASAKHQAY AALDLLDWPD GIYRYDIGSC AL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatg tattggtgat tggttctggc ggccgtgagc actcaatgtt gcatcacatt cgtaaatcta cattactaaa caagctattt atcgccccag ggcgtgaagg aatgtctggg ttagcagata taatagatat agatatcaat agcacaatag aagtaattca agtatgtaag aaagaaaaaa ttgaattagt agtcatcgga ccagaaactc cattaatgaa tggattatca gacgcattaa cagaagaagg catattagtc tttggacctt ctaaagcagc agcgcgtctt gaatcttcaa aaggatttac aaaagaatta tgcatgaggt atggaattcc tactgcaaaa tacgggtact ttgttgatac aaattcagct tacaaattca ttgataaaca caaattacct ttagtagtta aggctgatgg gttagcccag ggaaaaggaa cagtgatatg tcacacacac gaaaaagcat acaatgctgt agatgccatg ttagtgcacc acaaatttgg agaagctggt tgtgcaataa ttattgaaga attccttgaa ggcaaggaaa ttagcttctt tacattggtt gacggatcca acccagttat acttggcgta gcacaagatt ataaaactat aggagataat aataaaggtc ctaatactgg agggatggga tcatactcta aaccaaatat cattacacaa gaaatggagc atataataat tcagaagata atatatccaa ctattaaagc aatgttcaac atgaatatac agtttagagg tctgttattc gctggtatta taatcaaaaa aaatgaacca aaattacttg aatataatgt acggtttgga gatcctgaaa cacaatcaat attaccaaga ttaaattccg atttcttaaa acttttatca ctaacagcta aaggtaaact aggaaatgaa tcagtagaat taagtaaaaa agctgcttta tgtgttgtgg tagctagtcg tggatatcca ggtgagtata agaaaaattc tataattaat ggaatagaaa atattgaaaa gctacctaat gttcagctct tacatgcagg cacaagaaga gaaggaaata actgggtatc agattctgga agagtaataa atgttgtagc acaaggtgaa aatttagcta gtgcgaaaca ccaagcctac gctgcattgg acttattaga ttggccagat ggaatttaca gatatgatat aggatcatgt gctctttaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNVLVIGSG GREHSMLHHI RKSTLLNKLF IAPGREGMSG LADIIDIDIN STIEVIQVCK KEKIELVVIG PETPLMNGLS DALTEEGILV FGPSKAAARL ESSKGFTKEL CMRYGIPTAK YGYFVDTNSA YKFIDKHKLP LVVKADGLAQ GKGTVICHTH EEAYNAVDAM LVHHKFGEAG CAIIIEEFLE GKEISFFTLV DGSNPVILGV AQDYKTIGDN NKGPNTGGMG SYSKPNIITQ EMEHIIIQKI IYPTIKAMFN MNIQFRGLLF AGIIIKKNEP KLLEYNVRFG DPETQSILPR LNSDFLKLLS LTAKGKLGNE SVELSKKAAL CVVVASRGYP GEYKKNSIIN GIENIEKLPN VQLLHAGTRR EGNNWVSDSG RVINVVAQGE NLASAKHQAY AALDLLDWPD GIYRYDIGSC AL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATGTATTG GTGATTGGTT CTGGCGGCCG TGAGCACTCA ATGTTGCATC ACATTCGTAA ATCTACATTA CTAAACAAGC TATTTATCGC CCCAGGGCGT GAAGGAATGT CTGGGTTAGC AGATATAATA GATATAGATA TCAATAGCAC AATAGAAGTA ATTCAAGTAT GTAAGAAAGA AAAAATTGAA TTAGTAGTCA TCGGACCAGA AACTCCATTA ATGAATGGAT TATCAGACGC ATTAACAGAA GAAGGCATAT TAGTCTTTGG ACCTTCTAAA GCAGCAGCGC GTCTTGAATC TTCAAAAGGA TTTACAAAAG AATTATGCAT GAGGTATGGA ATTCCTACTG CAAAATACGG GTACTTTGTT GATACAAATT CAGCTTACAA ATTCATTGAT AAACACAAAT TACCTTTAGT AGTTAAGGCT GATGGGTTAG CCCAGGGAAA AGGAACAGTG ATATGTCACA CACACGAAGA AGCATACAAT GCTGTAGATG CCATGTTAGT GCACCACAAA TTTGGAGAAG CTGGTTGTGC AATAATTATT GAAGAATTCC TTGAAGGCAA GGAAATTAGC TTCTTTACAT TGGTTGACGG ATCCAACCCA GTTATACTTG GCGTAGCACA AGATTATAAA ACTATAGGAG ATAATAATAA AGGTCCTAAT ACTGGAGGGA TGGGATCATA CTCTAAACCA AATATCATTA CACAAGAAAT GGAGCATATA ATAATTCAGA AGATAATATA TCCAACTATT AAAGCAATGT TCAACATGAA TATACAGTTT AGAGGTCTGT TATTCGCTGG TATTATAATC AAAAAAAATG AACCAAAATT ACTTGAATAT AATGTACGGT TTGGAGATCC TGAAACACAA TCAATATTAC CAAGATTAAA TTCCGATTTC TTAAAACTTT TATCACTAAC AGCTAAAGGT AAACTAGGAA ATGAATCAGT AGAATTAAGT AAAAAAGCTG CTTTATGTGT TGTGGTAGCT AGTCGTGGAT ATCCAGGTGA GTATAAGAAA AATTCTATAA TTAATGGAAT AGAAAATATT GAAAAGCTAC CTAATGTTCA GCTCTTACAT GCAGGCACAA GAAGAGAAGG AAATAACTGG GTATCAGATT CTGGAAGAGT AATAAATGTT GTAGCACAAG GTGAAAATTT AGCTAGTGCG AAACACCAAG CCTACGCTGC ATTGGACTTA TTAGATTGGC CAGATGGAAT TTACAGATAT GATATAGGAT CATGTGCTCT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA