EhchA.00849.a

isocitrate dehydrogenase

CENTER ID: EhchA.00849.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00849.a.A1.GE26763 Full length( EhchA.00849.a ) 1 482
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.954
RefSeq: YP_507898.1
UniProt: Q2GF85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIPITVAYG DGIGPEIMEA VLLILSEAES GLVVETIEVG HNLYKKEWSS GIAPSSWDSI YRTKVLLKSP TMTPQGRGHK SLNVTLRKRL GLYANIRPCI SYHPVIKTRY PNLNVVIVRE NEEDTYTGIE HRLTNDTYQC SKVITRSGSE RICDYAFHYA KVHNRKRVTC LIKDNIMKMT DGIFHKSFSK IAENYPDIES DHYIVDIGMA KVASNPENFD VIVTTNLYGD IVSDIVAELS GSIGLAGSAN IGNNYAMFEA VHGSAPDIAG KNIANPSGLL NAAIQMLMYL KQFDKAQLIY NAFLKTLEDG IHTADIYQSQ VSKKKVSTMD FAKAVVENFG QSPSQLPKSM FQDNVDRTGV SYTYEPSYVT RVLVGVDITI GCDGVGLDFK QLINNLQNIT HDKLELVLIH NKGLEIWPDE SVSVNLSYMD QVCCRFYMKS KDDKIMNEHI NQLLFDIEQK KIDVVKMEKL YLYNDQPGFF TM
NT Sequence
ATGTCAATTC CAATAACAGT TGCTTATGGA GATGGTATTG GCCCAGAAAT TATGGAAGCT GTACTATTGA TCTTGAGTGA AGCAGAATCA GGTCTAGTTG TAGAAACTAT AGAGGTGGGA CACAATTTAT ATAAAAAAGA ATGGTCTTCT GGTATTGCGC CTTCATCTTG GGATTCTATT TATAGAACGA AAGTACTGCT TAAGTCTCCT ACCATGACTC CACAAGGTCG TGGGCATAAA AGTCTTAATG TTACGTTGAG AAAGAGATTA GGGTTGTATG CAAATATTAG GCCATGTATT TCTTATCATC CTGTGATAAA GACAAGGTAT CCTAATCTCA ATGTAGTGAT AGTTCGAGAA AATGAGGAAG ATACATATAC TGGTATAGAA CATAGGCTGA CTAATGATAC GTATCAATGC TCAAAAGTTA TTACGAGATC AGGTTCAGAA AGAATATGTG ATTATGCATT TCACTATGCT AAGGTTCATA ATAGGAAGAG AGTTACTTGC TTGATAAAAG ATAATATTAT GAAAATGACA GATGGAATTT TTCATAAGTC TTTTTCAAAG ATTGCAGAGA ATTATCCTGA CATTGAATCA GATCATTATA TTGTTGATAT TGGAATGGCA AAAGTGGCTT CTAACCCTGA AAATTTTGAT GTTATAGTTA CTACTAATCT TTATGGTGAT ATAGTGTCTG ATATAGTTGC TGAATTATCA GGGTCTATAG GCCTTGCAGG TAGTGCTAAT ATAGGGAATA ATTATGCAAT GTTTGAAGCT GTTCATGGTT CAGCTCCAGA TATAGCTGGA AAGAACATAG CAAATCCTTC TGGATTACTT AATGCAGCTA TACAAATGTT GATGTATTTA AAACAGTTTG ACAAGGCACA GCTAATTTAT AATGCATTTC TTAAAACTTT AGAAGATGGT ATTCATACAG CAGATATTTA CCAAAGTCAG GTTAGCAAGA AAAAAGTTTC TACAATGGAT TTTGCTAAGG CTGTTGTTGA AAATTTTGGA CAATCTCCAT CTCAATTACC AAAATCAATG TTTCAGGATA ATGTAGATAG GACGGGTGTA TCTTATACAT ATGAACCATC TTATGTTACT AGAGTTCTTG TAGGAGTGGA TATTACTATT GGTTGTGATG GTGTAGGACT AGATTTTAAG CAGTTAATTA ATAATCTTCA AAATATTACA CATGATAAAC TGGAGCTTGT GCTAATTCAT AATAAAGGAT TAGAAATTTG GCCTGATGAA TCTGTGAGTG TAAATCTTTC TTATATGGAT CAGGTATGTT GTAGGTTTTA CATGAAAAGT AAAGATGATA AAATCATGAA TGAGCATATT AATCAGTTGC TTTTTGACAT AGAGCAAAAG AAAATAGATG TAGTAAAAAT GGAAAAATTA TACTTATATA ATGATCAACC TGGCTTTTTT ACTATG
Details for EhchA.00849.a.A1.GE26763
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 56kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPITVAY GDGIGPEIME AVLLILSEAE SGLVVETIEV GHNLYKKEWS SGIAPSSWDS IYRTKVLLKS PTMTPQGRGH KSLNVTLRKR LGLYANIRPC ISYHPVIKTG YPNLNVVIVR ENEEDTYTGI EHRLTNDTYQ CSKVITRSGS ERICDYAFHY AKVRNRKRVT CLIKDNIMKM TDGIFHKSFS KIAENYPDIE SDHYIVDIXM AKVASNPENF DVIVTTNLYG DIVSDIVAEL SGSIXXAGSA NIGNNYAMFE AVHGSAPDIA GKNIANXSGL LNAAIQMLMY LKQFDKAQLI YNAFLKTLED GIHTADIYQS QVSKKKVSTM DFAKAVVENF GQSPSQLPKS MFQDNVDRTG VSYTYEPSYV TRVLVGVDIT IGCDGVGLDF KQLINNLQNI THDKLELVLI HNKGLEIWPD ESVSVNLSYM DQVCCRFYMK SKDDKIMNEH INQLLFDIEQ KKIDVVKMEK LYLYNDQPGF FTM
Validated NT Sequence
ttattacata gtaaaaaagc caggttgatc attatataag tataattttt ccatttttac tacatctatt ttcttttgct ctatgtcaaa aagcaactga ttaatatgct cattcatgat tttatcatct ttacttttca tgtaaaacct acaacatacc tgatccatat aagaaagatt tacactcaca gattcatcag gccaaatttc taatccttta ttatgaatta gcacaagctc cagtttatca tgtgtaatat tttgaagatt attaattaac tgcttaaaat ctagtcctac accatcacaa ccaatagtaa tatccactcc tacaagaact ctagtaacat aagatggttc atatgtataa gatacacccg tcctatctac attatcctga aacattgatt ttggtaattg agatggagat tgtccaaaat tttcaacaac agccttagca aaatccattg tagaaacttt tttcttgcta acctgacttt ggtaaatatc tgctgtatga ataccatctt ctaaagtttt aagaaatgca ttataaatta gctgtgcctt gtcaaactgt tttaaataca tcaacatttg tatagctgca ttaagtaatc cagaangatt tgctatgttc tttccagcta tatctggagc tgaaccatga acagcttcaa acattgcata attattccct atattagcac tacctgcann gnntatagac cctgataatt cagcaactat atcagatact atatcaccat aaagattagt agtaactata acatcaaaat tttcagggtt agaagccact tttgccattn caatatcaac aatataatga tctgattcaa tgtcaggata attctctgca atctttgaaa aagacttatg aaaaattcca tctgtcattt tcataatatt atcttttatc aagcaagtaa ctctcttcct attacgaacc ttagcatagt gaaatgcata atcacatatt ctttctgagc ctgatctcgt aataactttt gagcattgat acgtatcatt agtcagccta tgttctatac cagtatatgt atcttcctca ttttctcgaa ctatcactac attgagatta ggataccctg tctttatcac aggatgataa gaaatacatg gcctaatatt tgcatacaac cctaatctct ttctcaacgt aacattaaga cttttatgcc cacgaccttg tggagtcatg gtaggagact taagcagtac tttcgttcta taaatagaat cccaagatga aggcgcaata ccagaagacc attctttttt atataaattg tgtcccacct ctatagtttc tacaactaga cctgattctg cttcactcaa gatcaatagt acagcttcca taatttctgg gccaatacca tctccataag caactgttat tggaattgac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIPITVAY GDGIGPEIME AVLLILSEAE SGLVVETIEV GHNLYKKEWS SGIAPSSWDS IYRTKVLLKS PTMTPQGRGH KSLNVTLRKR LGLYANIRPC ISYHPVIKTR YPNLNVVIVR ENEEDTYTGI EHRLTNDTYQ CSKVITRSGS ERICDYAFHY AKVHNRKRVT CLIKDNIMKM TDGIFHKSFS KIAENYPDIE SDHYIVDIGM AKVASNPENF DVIVTTNLYG DIVSDIVAEL SGSIGLAGSA NIGNNYAMFE AVHGSAPDIA GKNIANPSGL LNAAIQMLMY LKQFDKAQLI YNAFLKTLED GIHTADIYQS QVSKKKVSTM DFAKAVVENF GQSPSQLPKS MFQDNVDRTG VSYTYEPSYV TRVLVGVDIT IGCDGVGLDF KQLINNLQNI THDKLELVLI HNKGLEIWPD ESVSVNLSYM DQVCCRFYMK SKDDKIMNEH INQLLFDIEQ KKIDVVKMEK LYLYNDQPGF FTM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaattcca ataacagttg cttatggaga tggtattggc ccagaaatta tggaagctgt actattgatc ttgagtgaag cagaatcagg tctagttgta gaaactatag aggtgggaca caatttatat aaaaaagaat ggtcttctgg tattgcgcct tcatcttggg attctattta tagaacgaaa gtactgctta agtctcctac catgactcca caaggtcgtg ggcataaaag tcttaatgtt acgttgagaa agagattagg gttgtatgca aatattaggc catgtatttc ttatcatcct gtgataaaga caaggtatcc taatctcaat gtagtgatag ttcgagaaaa tgaggaagat acatatactg gtatagaaca taggctgact aatgatacgt atcaatgctc aaaagttatt acgagatcag gttcagaaag aatatgtgat tatgcatttc actatgctaa ggttcataat aggaagagag ttacttgctt gataaaagat aatattatga aaatgacaga tggaattttt cataagtctt tttcaaagat tgcagagaat tatcctgaca ttgaatcaga tcattatatt gttgatattg gaatggcaaa agtggcttct aaccctgaaa attttgatgt tatagttact actaatcttt atggtgatat agtgtctgat atagttgctg aattatcagg gtctataggc cttgcaggta gtgctaatat agggaataat tatgcaatgt ttgaagctgt tcatggttca gctccagata tagctggaaa gaacatagca aatccttctg gattacttaa tgcagctata caaatgttga tgtatttaaa acagtttgac aaggcacagc taatttataa tgcatttctt aaaactttag aagatggtat tcatacagca gatatttacc aaagtcaggt tagcaagaaa aaagtttcta caatggattt tgctaaggct gttgttgaaa attttggaca atctccatct caattaccaa aatcaatgtt tcaggataat gtagatagga cgggtgtatc ttatacatat gaaccatctt atgttactag agttcttgta ggagtggata ttactattgg ttgtgatggt gtaggactag attttaagca gttaattaat aatcttcaaa atattacaca tgataaactg gagcttgtgc taattcataa taaaggatta gaaatttggc ctgatgaatc tgtgagtgta aatctttctt atatggatca ggtatgttgt aggttttaca tgaaaagtaa agatgataaa atcatgaatg agcatattaa tcagttgctt tttgacatag agcaaaagaa aatagatgta gtaaaaatgg aaaaattata cttatataat gatcaacctg gcttttttac tatgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa