EhchA.00827.a

response regulator PleD

CENTER ID: EhchA.00827.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00827.a.A1.GE26759 Full length( EhchA.00827.a ) 1 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.672
RefSeq: YP_507571.1
UniProt: Q2GG62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAKVLIVDD LPANIKLLQA KLMSEYYNVL TATNGMDAIK IAEEKHPDII LLDVMMPEMD GYETCKRLKS NPETTYIPIV MVTALDNTSD NRVSGLSYGA DDFLTKPIND TALFARIRSL TRFKMVIDEL RLRGKTNADM SLLHNNMMDY ANKIDDASIL IVDEDMIRSE HINNVLQKSF QQTVVSHNID QALTLSNTQN YDLIIIDLEF SGDGLRLCSQ LRNKIETRYT PILVLLDENE DPKLLSKAFD IGIHDYITNP IDSNELIARV NVQVKRKRYQ DALRINVDNS MAMAITDPLT GCYNRRYFDM HFHNIVNESL QKDKNLSLMI LDIDHFKEVN DTFGHTVGDE LLQQFKKRVS DNIRITDLLA RFGGEEFVVI LPDTTITTAE QIATRILSVI EETPFKTSIG DINKTVSIGV VEVQQSDINN IKQLVDRADK CLYEAKNTGR NKVVTYLS
NT Sequence
ATGACTGCAA AAGTATTAAT AGTAGATGAC TTACCTGCAA ATATAAAATT GTTGCAAGCT AAGCTCATGA GTGAATATTA TAACGTTCTA ACAGCAACAA ATGGGATGGA TGCAATTAAA ATTGCTGAAG AAAAACATCC AGACATAATA CTATTAGATG TTATGATGCC AGAAATGGAT GGATATGAAA CTTGCAAGAG ATTAAAATCT AATCCAGAAA CTACATATAT TCCAATAGTC ATGGTTACAG CTCTTGATAA TACATCAGAC AATAGAGTTA GTGGTCTCAG CTATGGCGCA GATGATTTCT TAACAAAACC TATAAATGAC ACAGCTTTAT TTGCACGTAT CAGATCATTA ACAAGATTCA AGATGGTTAT AGACGAATTA CGATTAAGAG GAAAAACAAA TGCAGATATG AGTTTATTAC ACAATAATAT GATGGACTAT GCAAATAAAA TTGACGATGC TAGCATCTTA ATTGTTGATG AAGATATGAT AAGATCTGAG CATATAAATA ACGTTTTACA AAAGTCTTTC CAACAAACTG TAGTTTCACA TAATATAGAT CAAGCATTAA CCCTAAGTAA TACACAAAAC TATGACTTAA TCATTATTGA TCTAGAATTT TCTGGAGATG GATTGAGGTT GTGCTCACAA CTACGTAACA AAATAGAAAC ACGCTATACT CCTATTCTAG TATTACTTGA TGAAAATGAG GATCCAAAAT TATTAAGTAA AGCTTTTGAC ATAGGAATCC ACGACTACAT TACAAACCCT ATAGACAGTA ACGAGCTTAT TGCAAGGGTA AACGTACAGG TAAAAAGGAA GAGATATCAA GATGCTTTAA GAATCAATGT AGATAACAGC ATGGCAATGG CTATAACTGA TCCTCTCACA GGGTGCTATA ACAGACGATA CTTTGATATG CATTTTCATA ACATAGTAAA TGAATCACTA CAAAAAGATA AAAATCTATC TTTAATGATA TTAGATATTG ACCATTTTAA AGAAGTCAAT GATACATTTG GACATACTGT TGGAGATGAG CTATTGCAAC AGTTTAAAAA ACGTGTATCA GACAATATAC GCATTACAGA CCTATTAGCA AGATTTGGTG GAGAAGAATT TGTTGTAATA TTACCAGATA CAACAATTAC TACAGCAGAA CAAATAGCTA CAAGAATTCT TTCTGTAATA GAAGAAACTC CTTTTAAAAC AAGTATAGGT GATATAAACA AAACCGTCAG TATTGGTGTA GTTGAAGTAC AACAGTCTGA CATTAATAAT ATAAAACAAC TAGTAGACAG GGCTGATAAG TGCCTATATG AAGCAAAAAA TACAGGAAGA AACAAAGTTG TCACATATCT ATCA
Details for EhchA.00827.a.A1.GE26759
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKVLIVD DLPANIKLLQ AKLMSEYYNV LTATNGMDAI KIAEEKHPDI ILLDVMMPEM DGYETCKRLK SNPETTYIPI VVVTALDNTS DNRVSGLSYG ADDFLTKPIN DTALFARIRS LTRFKMVIDE LRLRGKTNAD VSLLHNNMMD YANKIDDASI LIVDEDMIRS EHINNVLQKS FQQTVVSHNI DQALTLSNTQ NYDLIIIDLE FSGDGLRLCS QLRNKIETRY TPILVLLDEN EDPKLLSKAF DIGIHDYITN PIDSNELIAR VNVQVKRKRY QDALRINVDN SMAMAITDPL TGCYNRRYFD MHFHNIVNES LQKDKNLSLM ILDIDHFKEV NDTFGHTVGD ELLQQFKKRV SDNIRITDLL ARFGGEEFVV ILPDTTITTA EQIATRILSV IEETPFKTSI GDINKTVSIG VVEVQQPDIN NIKQLVDRAD KCLYEAKNTG RNKVVTYLS
Validated NT Sequence
ttattatgat agatatgtga caactttgtt tcttcctgta ttttttgctt catataggca cttatcagcc ctgtctacta gctgttttat attattaatg tcaggctgtt gtacttcaac tacaccaata ctgacggttt tgtttatatc acctatactt gttttaaaag gagtttcttc tattacagaa agaattcttg tagctatttg ttctgctgta gtaattgttg tatctggtaa tattacaaca aattcttctc caccaaatct tgctaatagg tctgtaatgc gtatattgtc tgatacacgt tttttaaact gttgcaatag ctcatctcca acagtatgtc caaatgtatc attgacttct ttaaaatggt caatatctaa tatcattaaa gatagatttt tatctttttg tagtgattca tttactatgt tatgaaaatg catatcaaag tatcgtctgt tatagcaccc tgtgagagga tcagttatag ccattgccat gctgttatct acattgattc ttaaagcatc ttgatatctc ttccttttta cctgtacgtt tacccttgca ataagctcgt tactgtctat agggtttgta atgtagtcgt ggattcctat gtcaaaagct ttacttaata attttggatc ctcattttca tcaagtaata ctaggatagg agtatagcgt gtttctattt tgttacgtag ttgtgagcac aacctcaatc catctccaga aaattctaga tcaataatga ttaagtcata gttttgtgta ttacttaggg ttaatgcttg atctatatta tgtgaaacta cagtttgttg gaaagacttt tgtaaaacgt tatttatatg ctcagatctt atcatatctt catcaacaat taagatgcta gcatcgtcaa ttttatttgc atagtccatc atattattgt gtaataaact cacatctgca tttgtttttc ctcttaatcg taattcgtct ataaccatct tgaatcttgt taatgatctg atacgtgcaa ataaagctgt gtcatttata ggttttgtta agaaatcatc tgcgccatag ctgagaccac taactctatt gtctgatgta ttatcaagag ctgtaaccac gactattgga atatatgtag tttctggatt agattttaat ctcttgcaag tttcatatcc atccatttct ggcatcataa catctaatag tattatgtct ggatgttttt cttcagcaat tttaattgca tccatcccat ttgttgctgt tagaacgtta taatattcac tcatgagctt agcttgcaac aattttatat ttgcaggtaa gtcatctact attaatactt ttgcagtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKVLIVD DLPANIKLLQ AKLMSEYYNV LTATNGMDAI KIAEEKHPDI ILLDVMMPEM DGYETCKRLK SNPETTYIPI VMVTALDNTS DNRVSGLSYG ADDFLTKPIN DTALFARIRS LTRFKMVIDE LRLRGKTNAD MSLLHNNMMD YANKIDDASI LIVDEDMIRS EHINNVLQKS FQQTVVSHNI DQALTLSNTQ NYDLIIIDLE FSGDGLRLCS QLRNKIETRY TPILVLLDEN EDPKLLSKAF DIGIHDYITN PIDSNELIAR VNVQVKRKRY QDALRINVDN SMAMAITDPL TGCYNRRYFD MHFHNIVNES LQKDKNLSLM ILDIDHFKEV NDTFGHTVGD ELLQQFKKRV SDNIRITDLL ARFGGEEFVV ILPDTTITTA EQIATRILSV IEETPFKTSI GDINKTVSIG VVEVQQSDIN NIKQLVDRAD KCLYEAKNTG RNKVVTYLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgcaaaa gtattaatag tagatgactt acctgcaaat ataaaattgt tgcaagctaa gctcatgagt gaatattata acgttctaac agcaacaaat gggatggatg caattaaaat tgctgaagaa aaacatccag acataatact attagatgtt atgatgccag aaatggatgg atatgaaact tgcaagagat taaaatctaa tccagaaact acatatattc caatagtcat ggttacagct cttgataata catcagacaa tagagttagt ggtctcagct atggcgcaga tgatttctta acaaaaccta taaatgacac agctttattt gcacgtatca gatcattaac aagattcaag atggttatag acgaattacg attaagagga aaaacaaatg cagatatgag tttattacac aataatatga tggactatgc aaataaaatt gacgatgcta gcatcttaat tgttgatgaa gatatgataa gatctgagca tataaataac gttttacaaa agtctttcca acaaactgta gtttcacata atatagatca agcattaacc ctaagtaata cacaaaacta tgacttaatc attattgatc tagaattttc tggagatgga ttgaggttgt gctcacaact acgtaacaaa atagaaacac gctatactcc tattctagta ttacttgatg aaaatgagga tccaaaatta ttaagtaaag cttttgacat aggaatccac gactacatta caaaccctat agacagtaac gagcttattg caagggtaaa cgtacaggta aaaaggaaga gatatcaaga tgctttaaga atcaatgtag ataacagcat ggcaatggct ataactgatc ctctcacagg gtgctataac agacgatact ttgatatgca ttttcataac atagtaaatg aatcactaca aaaagataaa aatctatctt taatgatatt agatattgac cattttaaag aagtcaatga tacatttgga catactgttg gagatgagct attgcaacag tttaaaaaac gtgtatcaga caatatacgc attacagacc tattagcaag atttggtgga gaagaatttg ttgtaatatt accagataca acaattacta cagcagaaca aatagctaca agaattcttt ctgtaataga agaaactcct tttaaaacaa gtataggtga tataaacaaa accgtcagta ttggtgtagt tgaagtacaa cagtctgaca ttaataatat aaaacaacta gtagacaggg ctgataagtg cctatatgaa gcaaaaaata caggaagaaa caaagttgtc acatatctat caaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa