EhchA.00820.a

dihydrodipicolinate reductase

CENTER ID: EhchA.00820.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00820.a.A1.GE26758 Full length( EhchA.00820.a ) 1 267
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00820.a.A1.PS00315 Full length( EhchA.00820.a ) 1 267

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.378
RefSeq: YP_507261.1
UniProt: Q2GH22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVNIGIVG CLGKQGRRLV SEISASPYAQ VSGGLVRSGN PHVGQILGEV VGCDCSVKIT DSLEHLFETS DIVIEFTNPD MLLECIKMAE QKKKPLLSGT TGPAATEMVF EDYIKSIPFL WTTNVSFGVN ILAKLVQEAA KKLFDYDIEI WEMHHRYKKD SPSGTSLILG KAAAKGRNVP FQMAQYVRGT PQEARKDVNT IGYSVSRGGA DLSDHRVMFV SDEEMIDFNH RTLNKNLYAK GALKAALWLV KQPPGVYTMS DMMAAVE
NT Sequence
ATGACACGAG TAAATATAGG TATAGTTGGT TGTTTGGGTA AACAAGGGAG AAGGTTGGTT AGTGAAATAT CAGCGAGTCC TTATGCTCAA GTAAGTGGGG GGCTTGTTCG TTCAGGAAAT CCACATGTTG GGCAAATTTT AGGAGAAGTT GTAGGATGTG ATTGTTCTGT CAAAATTACT GATTCTTTGG AACATCTTTT TGAAACTTCA GATATTGTTA TAGAATTTAC TAATCCTGAC ATGTTATTAG AGTGTATCAA AATGGCTGAA CAGAAGAAAA AGCCTTTATT AAGTGGTACT ACTGGTCCAG CTGCTACAGA AATGGTTTTT GAAGATTATA TTAAGAGTAT ACCATTTTTA TGGACTACAA ATGTAAGTTT TGGTGTTAAT ATACTTGCAA AATTGGTTCA AGAGGCTGCA AAGAAGCTGT TTGATTATGA TATTGAAATA TGGGAGATGC ATCATAGATA TAAGAAGGAT TCTCCATCTG GTACATCTTT AATTTTAGGA AAAGCTGCAG CTAAGGGAAG AAATGTTCCT TTCCAAATGG CTCAATATGT GAGAGGTACT CCACAGGAAG CTAGAAAAGA TGTGAATACT ATTGGATATT CTGTATCTAG AGGTGGTGCT GATTTAAGTG ATCATAGAGT CATGTTTGTC AGTGATGAGG AAATGATAGA CTTTAATCAT AGAACGTTAA ATAAAAACCT TTATGCAAAA GGAGCTTTAA AAGCTGCATT ATGGTTAGTC AAGCAACCTC CTGGTGTATA CACAATGTCA GATATGATGG CTGCTGTAGA G
Details for EhchA.00820.a.A1.GE26758
HARVESTED ON: 6/1/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVNIGIV GCLGKQGRRL VSEISASPYA QVSGGLVRSG NPHVGQILGE VVGCDCSVKI TDSLEHLFET SDIVIEFTNP DMLLECIKMA EQKKKPLLSG TTGPAATEMV FEDYIKSIPF LWTTNVSFGV NILAKLVQEA AKKLFDYDIE IWEMHHRYKK DSPSGTSLIL GKAAAKGRNV PFQMAQYVRG TPQEARKDVN TIGYSVSRGG ADLSDHRVMF VSDEEMIDFN HRTLNKNLYA KGALKAALWL VKQPPGVYTM SDMMAAVE
Validated NT Sequence
ttattactct acagcagcca tcatatctga cattgtgtat acaccaggag gttgcttgac taaccataat gcagctttta aagctccttt tgcataaagg tttttattta acgttctatg attaaagtct atcatttcct catcactgac aaacatgact ctatgatcac ttaaatcagc accacctcta gatacagaat atccaatagt attcacatct tttctagctt cctgtggagt acctctcaca tattgagcca tttggaaagg aacatttctt cccttagctg cagcttttcc taaaattaaa gatgtaccag atggagaatc cttcttatat ctatgatgca tctcccatat ttcaatatca taatcaaaca gcttctttgc agcctcttga accaattttg caagtatatt aacaccaaaa cttacatttg tagtccataa aaatggtata ctcttaatat aatcttcaaa aaccatttct gtagcagctg gaccagtagt accacttaat aaaggctttt tcttctgttc agccattttg atacactcta ataacatgtc aggattagta aattctataa caatatctga agtttcaaaa agatgttcca aagaatcagt aattttgaca gaacaatcac atcctacaac ttctcctaaa atttgcccaa catgtggatt tcctgaacga acaagccccc cacttacttg agcataagga ctcgctgata tttcactaac caaccttctc ccttgtttac ccaaacaacc aactatacct atatttactc gtgtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVNIGIV GCLGKQGRRL VSEISASPYA QVSGGLVRSG NPHVGQILGE VVGCDCSVKI TDSLEHLFET SDIVIEFTNP DMLLECIKMA EQKKKPLLSG TTGPAATEMV FEDYIKSIPF LWTTNVSFGV NILAKLVQEA AKKLFDYDIE IWEMHHRYKK DSPSGTSLIL GKAAAKGRNV PFQMAQYVRG TPQEARKDVN TIGYSVSRGG ADLSDHRVMF VSDEEMIDFN HRTLNKNLYA KGALKAALWL VKQPPGVYTM SDMMAAVE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacgagta aatataggta tagttggttg tttgggtaaa caagggagaa ggttggttag tgaaatatca gcgagtcctt atgctcaagt aagtgggggg cttgttcgtt caggaaatcc acatgttggg caaattttag gagaagttgt aggatgtgat tgttctgtca aaattactga ttctttggaa catctttttg aaacttcaga tattgttata gaatttacta atcctgacat gttattagag tgtatcaaaa tggctgaaca gaagaaaaag cctttattaa gtggtactac tggtccagct gctacagaaa tggtttttga agattatatt aagagtatac catttttatg gactacaaat gtaagttttg gtgttaatat acttgcaaaa ttggttcaag aggctgcaaa gaagctgttt gattatgata ttgaaatatg ggagatgcat catagatata agaaggattc tccatctggt acatctttaa ttttaggaaa agctgcagct aagggaagaa atgttccttt ccaaatggct caatatgtga gaggtactcc acaggaagct agaaaagatg tgaatactat tggatattct gtatctagag gtggtgctga tttaagtgat catagagtca tgtttgtcag tgatgaggaa atgatagact ttaatcatag aacgttaaat aaaaaccttt atgcaaaagg agctttaaaa gctgcattat ggttagtcaa gcaacctcct ggtgtataca caatgtcaga tatgatggct gctgtagaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for EhchA.00820.a.A1.PS00315
PURIFICATION DATe: 8/14/2009
CONCENTRATION: 48.12mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVNIGIV GCLGKQGRRL VSEISASPYA QVSGGLVRSG NPHVGQILGE VVGCDCSVKI TDSLEHLFET SDIVIEFTNP DMLLECIKMA EQKKKPLLSG TTGPAATEMV FEDYIKSIPF LWTTNVSFGV NILAKLVQEA AKKLFDYDIE IWEMHHRYKK DSPSGTSLIL GKAAAKGRNV PFQMAQYVRG TPQEARKDVN TIGYSVSRGG ADLSDHRVMF VSDEEMIDFN HRTLNKNLYA KGALKAALWL VKQPPGVYTM SDMMAAVE
Validated NT Sequence
ttattactct acagcagcca tcatatctga cattgtgtat acaccaggag gttgcttgac taaccataat gcagctttta aagctccttt tgcataaagg tttttattta acgttctatg attaaagtct atcatttcct catcactgac aaacatgact ctatgatcac ttaaatcagc accacctcta gatacagaat atccaatagt attcacatct tttctagctt cctgtggagt acctctcaca tattgagcca tttggaaagg aacatttctt cccttagctg cagcttttcc taaaattaaa gatgtaccag atggagaatc cttcttatat ctatgatgca tctcccatat ttcaatatca taatcaaaca gcttctttgc agcctcttga accaattttg caagtatatt aacaccaaaa cttacatttg tagtccataa aaatggtata ctcttaatat aatcttcaaa aaccatttct gtagcagctg gaccagtagt accacttaat aaaggctttt tcttctgttc agccattttg atacactcta ataacatgtc aggattagta aattctataa caatatctga agtttcaaaa agatgttcca aagaatcagt aattttgaca gaacaatcac atcctacaac ttctcctaaa atttgcccaa catgtggatt tcctgaacga acaagccccc cacttacttg agcataagga ctcgctgata tttcactaac caaccttctc ccttgtttac ccaaacaacc aactatacct atatttactc gtgtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVNIGIV GCLGKQGRRL VSEISASPYA QVSGGLVRSG NPHVGQILGE VVGCDCSVKI TDSLEHLFET SDIVIEFTNP DMLLECIKMA EQKKKPLLSG TTGPAATEMV FEDYIKSIPF LWTTNVSFGV NILAKLVQEA AKKLFDYDIE IWEMHHRYKK DSPSGTSLIL GKAAAKGRNV PFQMAQYVRG TPQEARKDVN TIGYSVSRGG ADLSDHRVMF VSDEEMIDFN HRTLNKNLYA KGALKAALWL VKQPPGVYTM SDMMAAVE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACGAGTA AATATAGGTA TAGTTGGTTG TTTGGGTAAA CAAGGGAGAA GGTTGGTTAG TGAAATATCA GCGAGTCCTT ATGCTCAAGT AAGTGGGGGG CTTGTTCGTT CAGGAAATCC ACATGTTGGG CAAATTTTAG GAGAAGTTGT AGGATGTGAT TGTTCTGTCA AAATTACTGA TTCTTTGGAA CATCTTTTTG AAACTTCAGA TATTGTTATA GAATTTACTA ATCCTGACAT GTTATTAGAG TGTATCAAAA TGGCTGAACA GAAGAAAAAG CCTTTATTAA GTGGTACTAC TGGTCCAGCT GCTACAGAAA TGGTTTTTGA AGATTATATT AAGAGTATAC CATTTTTATG GACTACAAAT GTAAGTTTTG GTGTTAATAT ACTTGCAAAA TTGGTTCAAG AGGCTGCAAA GAAGCTGTTT GATTATGATA TTGAAATATG GGAGATGCAT CATAGATATA AGAAGGATTC TCCATCTGGT ACATCTTTAA TTTTAGGAAA AGCTGCAGCT AAGGGAAGAA ATGTTCCTTT CCAAATGGCT CAATATGTGA GAGGTACTCC ACAGGAAGCT AGAAAAGATG TGAATACTAT TGGATATTCT GTATCTAGAG GTGGTGCTGA TTTAAGTGAT CATAGAGTCA TGTTTGTCAG TGATGAGGAA ATGATAGACT TTAATCATAG AACGTTAAAT AAAAACCTTT ATGCAAAAGG AGCTTTAAAA GCTGCATTAT GGTTAGTCAA GCAACCTCCT GGTGTATACA CAATGTCAGA TATGATGGCT GCTGTAGAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A