EhchA.00809.a

argininosuccinate synthase

CENTER ID: EhchA.00809.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00809.a.A1.GE26757 Full length( EhchA.00809.a ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.594
RefSeq: YP_507487.1
UniProt: Q2GGE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKIVLAYSG GLDTSVILKW LQENYNCEVV VFTADLGQND DMSAIRQKAA ASNVKEIFIE DLKEEFVKDF VFPMFRANTV YEGYYLLGTS IARPLIAKRQ IEIAHLTGAD AVAHGATGKG NDQIRFEFGY YSCDPNIKVI APWRQWELTS RNSLIEYAKK HGIDVPLDKA SEPPYSIDAN LLHTSYEGKS LEDPYIEPDY TMLSKSVIPE LASDTPEYIE ISFKNGDPHA INNVPLSPAN LLQQLNIIGG RHGIGIIDIV ENRYIGIKSR GVYETPGGTI LLHAHRAIES ITLDREAAHL KDEIMPRYAK LIYNGYWWTT ERKMLQALID KSQDKVNGVV RLKLYKGSVM VVGRTSENSL YSHNLASFDS SGNYNHTDAE GFIKINSLRL RNS
NT Sequence
ATGAAAAAAA TAGTATTAGC TTATTCAGGG GGATTAGATA CCTCAGTTAT ATTAAAATGG CTCCAAGAAA ATTATAATTG TGAAGTAGTA GTATTTACTG CAGATTTAGG TCAAAATGAT GATATGTCTG CAATAAGACA AAAGGCAGCT GCATCAAATG TAAAAGAAAT TTTTATAGAA GACTTAAAAG AAGAATTTGT AAAAGATTTC GTATTCCCCA TGTTTAGAGC AAATACTGTA TATGAAGGAT ATTACTTATT GGGAACATCT ATAGCAAGGC CATTAATAGC TAAACGACAA ATTGAAATAG CTCACCTTAC AGGAGCAGAT GCTGTAGCAC ATGGAGCAAC TGGAAAAGGA AATGACCAGA TTAGATTTGA ATTTGGATAT TATTCATGCG ATCCAAATAT AAAGGTAATA GCACCTTGGC GTCAGTGGGA ATTGACTTCA AGAAATAGTT TAATAGAATA TGCAAAAAAG CATGGTATTG ATGTTCCTTT AGATAAAGCA AGTGAACCAC CATATTCTAT AGATGCAAAC CTATTACATA CCTCATACGA AGGCAAATCT TTAGAAGATC CATATATTGA ACCTGATTAT ACAATGCTCT CCAAATCAGT AATTCCAGAA TTAGCTAGTG ATACTCCAGA ATATATAGAA ATTTCCTTTA AAAACGGAGA TCCACATGCA ATAAATAATG TACCCCTTTC ACCTGCAAAT CTATTACAAC AATTGAATAT AATAGGTGGT AGACATGGTA TAGGAATTAT TGACATAGTA GAAAATCGCT ATATAGGCAT AAAATCAAGA GGAGTATACG AAACTCCTGG AGGAACAATT CTTTTACACG CACACCGTGC TATAGAAAGT ATAACTTTAG ACAGAGAAGC AGCACACCTT AAGGATGAAA TAATGCCAAG ATATGCTAAA CTAATATATA ATGGATATTG GTGGACTACT GAAAGAAAGA TGTTACAAGC ACTTATTGAT AAATCACAAG ACAAAGTAAA TGGAGTAGTA CGCTTAAAAT TATACAAAGG ATCTGTTATG GTTGTAGGAC GAACATCAGA AAATAGCTTA TATTCACATA ACCTAGCAAG CTTTGACTCA TCAGGAAATT ATAATCATAC AGACGCAGAA GGATTTATTA AAATAAATTC TCTAAGATTA AGAAATAGC
Details for EhchA.00809.a.A1.GE26757
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MKKIVLAYSG GLDTSVILKW LQENYNCEVV VFTADSGQND DMSAIRQKAA ASNVKEIFIE DLKEEFVKDF VFPMFRANTV YEGYYLLGTS IARPLIAKRQ IEIAHLTGAD AVAHEATGKG NDQIRFEFGY YSCDPNIKVI APWRQWELTS RNSLIEYAKK HGIDVPLDKA SEPPYSIDAN LLHTSYEGKS LEDPYIEPDY TMLSKSVIPE LASDTPEYIE ISFKNGDPHA INNVPLSPAN LLQQLNIIGG RHGIGIIDIV ENRYIGIKSR GVYETPGGTI LLHAHRVIES ITLDREAAHL KDEIMPRYAK LIYNGYWWTT ERKMLQALID KSQDKVNGVV RLKLYKGSVM VVGRTSENSL YSHNLASFDS SGNYNHTDAE GFIKINSLRL R
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atgaaaaaaa tagtattagc ttattcaggg ggattagata cctcagttat attaaaatgg ctccaagaaa attataattg tgaagtagta gtatttactg cagattcagg tcaaaatgat gatatgtctg caataagaca aaaggcagct gcatcaaatg taaaagaaat ttttatagaa gacttaaaag aagaatttgt aaaagatttc gtattcccca tgtttagagc aaatactgta tatgaaggat attacttatt gggaacatct atagcaaggc cattaatagc taaacgacaa attgaaatag ctcaccttac aggagcagat gctgtagcac atgaagcaac tggaaaagga aatgaccaga ttagatttga atttggatat tattcatgcg atccaaatat aaaggtaata gcaccttggc gtcagtggga attgacttca agaaatagtt taatagaata tgcaaaaaag catggtattg atgttccttt agataaagca agtgaaccac catattctat agatgcaaac ctattacata cctcatacga aggcaaatcc ttagaagatc catatattga acctgattat acaatgctct ccaaatcagt aattccagaa ttagctagtg atactccaga atatatagaa atttccttta aaaacggaga tccacatgca ataaataatg tacccctttc acctgcaaat ctattacaac aattgaatat aataggtggt agacatggta taggaattat tgacatagta gaaaatcgct atataggcat aaaatcaaga ggagtatacg aaactcctgg aggaacaatt cttttacacg cacaccgtgt tatagaaagt ataactttag acagagaagc agcacacctt aaggatgaaa taatgccaag atatgctaaa ctaatatata atggatattg gtggactact gaaagaaaga tgttacaagc acttattgat aaatcacaag acaaagtaaa tggagtagta cgcttaaaat tatacaaagg atctgttatg gttgtaggac gaacatcaga aaatagctta tattcacata acctagcaag ctttgactca tcaggaaatt ataatcatac agacgcagaa ggatttatta aaataaattc tctaagatta agaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKIVLAYS GGLDTSVILK WLQENYNCEV VVFTADLGQN DDMSAIRQKA AASNVKEIFI EDLKEEFVKD FVFPMFRANT VYEGYYLLGT SIARPLIAKR QIEIAHLTGA DAVAHGATGK GNDQIRFEFG YYSCDPNIKV IAPWRQWELT SRNSLIEYAK KHGIDVPLDK ASEPPYSIDA NLLHTSYEGK SLEDPYIEPD YTMLSKSVIP ELASDTPEYI EISFKNGDPH AINNVPLSPA NLLQQLNIIG GRHGIGIIDI VENRYIGIKS RGVYETPGGT ILLHAHRAIE SITLDREAAH LKDEIMPRYA KLIYNGYWWT TERKMLQALI DKSQDKVNGV VRLKLYKGSV MVVGRTSENS LYSHNLASFD SSGNYNHTDA EGFIKINSLR LRNS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaaaaata gtattagctt attcaggggg attagatacc tcagttatat taaaatggct ccaagaaaat tataattgtg aagtagtagt atttactgca gatttaggtc aaaatgatga tatgtctgca ataagacaaa aggcagctgc atcaaatgta aaagaaattt ttatagaaga cttaaaagaa gaatttgtaa aagatttcgt attccccatg tttagagcaa atactgtata tgaaggatat tacttattgg gaacatctat agcaaggcca ttaatagcta aacgacaaat tgaaatagct caccttacag gagcagatgc tgtagcacat ggagcaactg gaaaaggaaa tgaccagatt agatttgaat ttggatatta ttcatgcgat ccaaatataa aggtaatagc accttggcgt cagtgggaat tgacttcaag aaatagttta atagaatatg caaaaaagca tggtattgat gttcctttag ataaagcaag tgaaccacca tattctatag atgcaaacct attacatacc tcatacgaag gcaaatcttt agaagatcca tatattgaac ctgattatac aatgctctcc aaatcagtaa ttccagaatt agctagtgat actccagaat atatagaaat ttcctttaaa aacggagatc cacatgcaat aaataatgta cccctttcac ctgcaaatct attacaacaa ttgaatataa taggtggtag acatggtata ggaattattg acatagtaga aaatcgctat ataggcataa aatcaagagg agtatacgaa actcctggag gaacaattct tttacacgca caccgtgcta tagaaagtat aactttagac agagaagcag cacaccttaa ggatgaaata atgccaagat atgctaaact aatatataat ggatattggt ggactactga aagaaagatg ttacaagcac ttattgataa atcacaagac aaagtaaatg gagtagtacg cttaaaatta tacaaaggat ctgttatggt tgtaggacga acatcagaaa atagcttata ttcacataac ctagcaagct ttgactcatc aggaaattat aatcatacag acgcagaagg atttattaaa ataaattctc taagattaag aaatagcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa