EhchA.00725.a

4-diphosphocytidyl-2C-methyl-D-erythritol kinase (IspE)

CENTER ID:	EhchA.00725.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00725.a.A1.GE26781	Full length( EhchA.00725.a )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130885
PATRIC ID:	fig\|205920.11.peg.658
RefSeq:	YP_507555.1
UniProt:	Q2GG78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKFLVKAPA KVNLFLHITG KRSDQHHYLE SLFVFVNVYD ILEVDVGGSK RGVYFSNLRI SKYNNTVYKA IELLLKHSAV CPNVSVSIIK NILVSAGLAG GSADAAAIMR LLGNMWNIDY TLLQDLALKI GSDVPACLES KTLFAKGVGE DILLLPDLLL PKYIILVAPR GKTLSTAKVF NNYQSATYSP SICDKLPVKQ DDWMELICNA KNDLLEVALK FVPEIEEILF VLKQLKNSVI ARMTGSGATC FALFNELSHA EDAARKLQMT RPDWIIFNAK IL

NT Sequence

ATGTTAAAGT TTTTAGTTAA AGCACCAGCA AAAGTTAATT TATTTTTGCA TATTACTGGT AAACGGAGTG ATCAACATCA TTACTTAGAG TCATTGTTTG TCTTTGTTAA TGTTTACGAT ATCTTAGAGG TTGATGTTGG TGGTTCGAAA CGGGGAGTAT ATTTCTCAAA TCTGAGGATT AGCAAATATA ATAATACTGT ATATAAAGCA ATAGAGTTGT TATTGAAACA CAGTGCTGTA TGTCCTAATG TTTCTGTGAG TATTATAAAA AATATTTTAG TATCTGCAGG TTTAGCAGGT GGATCAGCTG ATGCTGCTGC TATTATGCGT TTACTAGGTA ATATGTGGAA CATTGATTAC ACATTGTTAC AGGATTTAGC CTTAAAAATT GGAAGTGATG TTCCAGCATG TTTGGAATCA AAGACCCTTT TTGCTAAAGG GGTAGGGGAA GATATATTGT TATTACCTGA TTTGTTATTG CCAAAATATA TTATACTTGT TGCTCCAAGG GGAAAGACGT TAAGTACAGC AAAGGTCTTT AATAATTACC AGAGTGCTAC TTACTCTCCT TCCATATGTG ATAAGCTTCC TGTAAAGCAA GATGATTGGA TGGAATTAAT TTGTAATGCT AAAAATGATT TATTAGAAGT AGCGTTGAAA TTTGTTCCTG AGATAGAAGA AATATTATTT GTATTAAAGC AATTAAAAAA TTCTGTAATT GCTCGTATGA CTGGTAGTGG AGCAACTTGT TTTGCTTTAT TTAATGAATT AAGTCATGCT GAAGATGCAG CTAGAAAATT GCAGATGACA CGTCCTGATT GGATAATTTT TAATGCTAAA ATACTT

Details for EhchA.00725.a.A1.GE26781

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/1/2009
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKFLVKAP AKVNLFLHIT GKRSDQHHYL ESLFVFVNVY DILEVDVGGS KRGVYFSNLR ISKYNNTVYK AIELLLKHSA VCPNVSVSII KNILVSAGLA GGSADAAAIM RLLGNMWNID YTLLQDLALK IGSDVPACLE SKTLFAKGVG EDILLLPDLL LPKYIILVAP RGKTLSTAKV FNNYQSATYS PSICDKLPVK QDDWMELICN AKNDLLEVAL KFVPEIEEIL FVLKQLKNSV IARMTGSGAT CFALFNELSH AEDAARKLQM TRPDWIIFNA KIL

Validated NT Sequence

ttattaaagt attttagcat taaaaattat ccaatcagga cgtgtcatct gcaattttct agctgcatct tcagcatgac ttaattcatt aaataaagca aaacaagttg ctccactacc agtcatacga gcaattacag aattttttaa ttgctttaat acaaataata tttcttctat ctcaggaaca aatttcaacg ctacttctaa taaatcattt ttagcattac aaattaattc catccaatca tcttgcttta caggaagctt atcacatatg gaaggagagt aagtagcact ctggtaatta ttaaagacct ttgctgtact taacgtcttt ccccttggag caacaagtat aatatatttt ggcaataaca aatcaggtaa taacaatata tcttccccta cccctttagc aaaaagggtc tttgattcca aacatgctgg aacatcactt ccaattttta aggctaaatc ctgtaacaat gtgtaatcaa tgttccacat attacctagt aaacgcataa tagcagcagc atcagctgat ccacctgcta aacctgcaga tactaaaata ttttttataa tactcacaga aacattagga catacagcac tgtgtttcaa taacaactct attgctttat atacagtatt attatatttg ctaatcctca gatttgagaa atatactccc cgtttcgaac caccaacatc aacctctagg atatcgtaaa cattaacaaa gacaaacaat gactctaagt aatgatgttg atcactccgt ttaccagtaa tatgcaaaaa taaattaact tttgctggtg ctttaactaa aaactttaac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttaaagttt ttagttaaag caccagcaaa agttaattta tttttgcata ttactggtaa acggagtgat caacatcatt acttagagtc attgtttgtc tttgttaatg tttacgatat cttagaggtt gatgttggtg gttcgaaacg gggagtatat ttctcaaatc tgaggattag caaatataat aatactgtat ataaagcaat agagttgtta ttgaaacaca gtgctgtatg tcctaatgtt tctgtgagta ttataaaaaa tattttagta tctgcaggtt tagcaggtgg atcagctgat gctgctgcta ttatgcgttt actaggtaat atgtggaaca ttgattacac attgttacag gatttagcct taaaaattgg aagtgatgtt ccagcatgtt tggaatcaaa gacccttttt gctaaagggg taggggaaga tatattgtta ttacctgatt tgttattgcc aaaatatatt atacttgttg ctccaagggg aaagacgtta agtacagcaa aggtctttaa taattaccag agtgctactt actctccttc catatgtgat aagcttcctg taaagcaaga tgattggatg gaattaattt gtaatgctaa aaatgattta ttagaagtag cgttgaaatt tgttcctgag atagaagaaa tattatttgt attaaagcaa ttaaaaaatt ctgtaattgc tcgtatgact ggtagtggag caacttgttt tgctttattt aatgaattaa gtcatgctga agatgcagct agaaaattgc agatgacacg tcctgattgg ataattttta atgctaaaat acttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa