EhchA.00686.a

histidyl-tRNA synthetase

CENTER ID: EhchA.00686.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00686.a.A1.GE26777 Full length( EhchA.00686.a ) 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00686.a.A1.PS00316 Full length( EhchA.00686.a ) 1 413
EhchA.00686.a.A1.PW26852 Full length( EhchA.00686.a ) 1 413

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.245
RefSeq: YP_507112.1
UniProt: Q2GHH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRDKLREVR GTKDLLGIEL YKFQYIHRLS QSIAHRYGFI AVDTPIIEFT EVFTKTLGDD SDIVMKEMYN FQDKSGENIT LRPEFTSAIV RVLINKNLII PVKLFSSGPV FRYERPQKCR QRQFHQVNFE FFGSDLPLAD IEMIALGYHI LDELKLLDDI TLEINFLGDK ETMNSYKLSL IEYLNKYKKD LSEDSQRRLI TNPLRILDSK APEDREILLN VPNISYFYSK SSNDFFAEVL YGLDELCIPY KVNHSIVRGL DYYCNTVFEF TTSKLGAQNA VVAGGRYDGL VKSMGGNDTP AVGFAMGVER VSALIDYKHQ EPRSVVLIPI GKDAVSYALK LAYELRCNGI SVNWNYKNAN LRNALRKVGD DNIVLIFGDE ELKNNTIQVK DMKTGEQQEI VRCDLLDTLC NKI
NT Sequence
ATGCAGCGTG ATAAGTTAAG AGAAGTAAGG GGTACCAAGG ATCTATTAGG GATTGAACTT TATAAGTTTC AATATATACA TCGTTTATCT CAGTCAATAG CGCATAGATA TGGATTTATA GCTGTTGATA CTCCAATCAT TGAATTTACT GAAGTGTTTA CTAAGACTTT AGGTGATGAT TCAGATATTG TAATGAAGGA AATGTATAAT TTTCAGGATA AAAGTGGTGA AAATATTACA TTAAGGCCAG AATTTACATC TGCTATTGTG AGGGTATTAA TTAATAAAAA TTTAATTATT CCTGTCAAAT TATTTTCTAG TGGACCAGTA TTTCGTTATG AACGTCCTCA AAAGTGTCGT CAAAGACAAT TTCATCAAGT TAATTTTGAG TTTTTTGGAA GTGATCTTCC TCTTGCAGAT ATTGAAATGA TAGCATTAGG CTATCATATT TTGGATGAGT TGAAGTTGTT AGATGATATA ACGTTAGAGA TTAATTTTCT AGGTGATAAG GAAACTATGA ACTCATATAA GCTCAGTCTT ATAGAGTATT TAAACAAATA TAAGAAAGAT TTATCAGAAG ATAGTCAGCG TAGGCTTATT ACAAATCCTC TAAGAATATT AGATTCAAAA GCTCCAGAAG ATCGTGAAAT TTTATTGAAT GTTCCTAATA TTTCTTATTT TTATAGTAAA TCCTCTAATG ATTTTTTTGC AGAGGTTCTA TATGGGTTAG ATGAGCTCTG TATTCCTTAT AAAGTAAATC ATAGTATAGT TAGAGGTTTA GATTATTATT GTAATACGGT GTTTGAGTTT ACAACGTCTA AATTAGGAGC ACAAAATGCA GTTGTTGCTG GTGGTAGATA TGATGGATTA GTGAAGTCTA TGGGAGGAAA TGATACTCCT GCTGTTGGTT TTGCTATGGG TGTTGAAAGA GTTAGTGCGT TGATAGATTA TAAACATCAA GAACCTAGGA GTGTAGTATT GATACCTATA GGGAAAGATG CAGTATCCTA TGCTTTGAAG CTTGCTTATG AGTTACGTTG TAATGGTATT TCTGTAAACT GGAATTATAA AAATGCTAAT TTGAGAAATG CATTAAGGAA AGTAGGTGAT GATAATATAG TGTTAATTTT TGGAGATGAA GAGCTAAAAA ATAATACTAT ACAAGTCAAA GATATGAAAA CTGGTGAACA ACAAGAAATA GTTAGATGTG ATTTATTGGA CACATTGTGT AACAAAATT
Details for EhchA.00686.a.A1.GE26777
HARVESTED ON: 6/8/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLGNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcagc gtgataagtt aagagaagta aggggtacca aggatctatt agggattgaa ctttataagt ttcaatatat acatcgttta tctcagtcaa tagcgcatag atatggattt atagctgttg atactccaat cattgaattt actgaagtgt ttactaagac tttaggtgat gattcagata ttgtaatgaa ggaaatgtat aattttcagg ataaaagtgg tgaaaatatt acattaaggc cagaatttac atctgctatt gtgagggtat taattaataa aaatttaatt attcctgtca aattattttc tagtggacca gtatttcgtt atgaacgtcc tcaaaagtgt cgtcaaagac aatttcatca agttaatttt gagttttttg gaagtgatct tcctcttgca gatattgaaa tgatagcatt aggctatcat attttggatg agttgaagtt gttagatgat ataacgttag agattaattt tctaggtgat aaggaaacta tgaactcata taagctcagt cttatagagt atttaaacaa atataagaaa gatttatcag aagatagtca gcgtaggctt attacaaatc ctctaagaat attagattca aaagctccag aagatcgtga aattttattg aatgttccta atatttctta tttttatagt aaatcctcta atgatttttt tgcagaggtt ctatatgggt tagatgagct ctgtattcct tataaagtaa atcatagtat agttagaggt ttagattatt attgtaatac ggtgtttgag tttacaacgt ctaaattagg agcacaaaat gcagttgttg ctggtggtag atatgatgga ttagtgaagt ctatgggagg aaatgatact cctgctgttg gttttgctat gggtgttgaa agagttagtg cgttgataga ttataaacat caagaaccta ggagtgtagt attgatacct atagggaaag atgcagtatc ctatgctttg aagcttgctt atgagttacg ttgtaatggt atttctgtaa actggaatta taaaaatgct aatttgggaa atgcattaag gaaagtaggt gatgataata tagtgttaat ttttggagat gaagagctaa aaaataatac tatacaagtc aaagatatga aaactggtga acaacaagaa atagttagat gtgatttatt ggacacattg tgtaaca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLRNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CNKI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagcgtgat aagttaagag aagtaagggg taccaaggat ctattaggga ttgaacttta taagtttcaa tatatacatc gtttatctca gtcaatagcg catagatatg gatttatagc tgttgatact ccaatcattg aatttactga agtgtttact aagactttag gtgatgattc agatattgta atgaaggaaa tgtataattt tcaggataaa agtggtgaaa atattacatt aaggccagaa tttacatctg ctattgtgag ggtattaatt aataaaaatt taattattcc tgtcaaatta ttttctagtg gaccagtatt tcgttatgaa cgtcctcaaa agtgtcgtca aagacaattt catcaagtta attttgagtt ttttggaagt gatcttcctc ttgcagatat tgaaatgata gcattaggct atcatatttt ggatgagttg aagttgttag atgatataac gttagagatt aattttctag gtgataagga aactatgaac tcatataagc tcagtcttat agagtattta aacaaatata agaaagattt atcagaagat agtcagcgta ggcttattac aaatcctcta agaatattag attcaaaagc tccagaagat cgtgaaattt tattgaatgt tcctaatatt tcttattttt atagtaaatc ctctaatgat ttttttgcag aggttctata tgggttagat gagctctgta ttccttataa agtaaatcat agtatagtta gaggtttaga ttattattgt aatacggtgt ttgagtttac aacgtctaaa ttaggagcac aaaatgcagt tgttgctggt ggtagatatg atggattagt gaagtctatg ggaggaaatg atactcctgc tgttggtttt gctatgggtg ttgaaagagt tagtgcgttg atagattata aacatcaaga acctaggagt gtagtattga tacctatagg gaaagatgca gtatcctatg ctttgaagct tgcttatgag ttacgttgta atggtatttc tgtaaactgg aattataaaa atgctaattt gagaaatgca ttaaggaaag taggtgatga taatatagtg ttaatttttg gagatgaaga gctaaaaaat aatactatac aagtcaaaga tatgaaaact ggtgaacaac aagaaatagt tagatgtgat ttattggaca cattgtgtaa caaaattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for EhchA.00686.a.A1.PS00316
PURIFICATION DATe: 8/13/2009
CONCENTRATION: 13.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLGNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcagc gtgataagtt aagagaagta aggggtacca aggatctatt agggattgaa ctttataagt ttcaatatat acatcgttta tctcagtcaa tagcgcatag atatggattt atagctgttg atactccaat cattgaattt actgaagtgt ttactaagac tttaggtgat gattcagata ttgtaatgaa ggaaatgtat aattttcagg ataaaagtgg tgaaaatatt acattaaggc cagaatttac atctgctatt gtgagggtat taattaataa aaatttaatt attcctgtca aattattttc tagtggacca gtatttcgtt atgaacgtcc tcaaaagtgt cgtcaaagac aatttcatca agttaatttt gagttttttg gaagtgatct tcctcttgca gatattgaaa tgatagcatt aggctatcat attttggatg agttgaagtt gttagatgat ataacgttag agattaattt tctaggtgat aaggaaacta tgaactcata taagctcagt cttatagagt atttaaacaa atataagaaa gatttatcag aagatagtca gcgtaggctt attacaaatc ctctaagaat attagattca aaagctccag aagatcgtga aattttattg aatgttccta atatttctta tttttatagt aaatcctcta atgatttttt tgcagaggtt ctatatgggt tagatgagct ctgtattcct tataaagtaa atcatagtat agttagaggt ttagattatt attgtaatac ggtgtttgag tttacaacgt ctaaattagg agcacaaaat gcagttgttg ctggtggtag atatgatgga ttagtgaagt ctatgggagg aaatgatact cctgctgttg gttttgctat gggtgttgaa agagttagtg cgttgataga ttataaacat caagaaccta ggagtgtagt attgatacct atagggaaag atgcagtatc ctatgctttg aagcttgctt atgagttacg ttgtaatggt atttctgtaa actggaatta taaaaatgct aatttgggaa atgcattaag gaaagtaggt gatgataata tagtgttaat ttttggagat gaagagctaa aaaataatac tatacaagtc aaagatatga aaactggtga acaacaagaa atagttagat gtgatttatt ggacacattg tgtaaca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLRNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CNKI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGCGTGAT AAGTTAAGAG AAGTAAGGGG TACCAAGGAT CTATTAGGGA TTGAACTTTA TAAGTTTCAA TATATACATC GTTTATCTCA GTCAATAGCG CATAGATATG GATTTATAGC TGTTGATACT CCAATCATTG AATTTACTGA AGTGTTTACT AAGACTTTAG GTGATGATTC AGATATTGTA ATGAAGGAAA TGTATAATTT TCAGGATAAA AGTGGTGAAA ATATTACATT AAGGCCAGAA TTTACATCTG CTATTGTGAG GGTATTAATT AATAAAAATT TAATTATTCC TGTCAAATTA TTTTCTAGTG GACCAGTATT TCGTTATGAA CGTCCTCAAA AGTGTCGTCA AAGACAATTT CATCAAGTTA ATTTTGAGTT TTTTGGAAGT GATCTTCCTC TTGCAGATAT TGAAATGATA GCATTAGGCT ATCATATTTT GGATGAGTTG AAGTTGTTAG ATGATATAAC GTTAGAGATT AATTTTCTAG GTGATAAGGA AACTATGAAC TCATATAAGC TCAGTCTTAT AGAGTATTTA AACAAATATA AGAAAGATTT ATCAGAAGAT AGTCAGCGTA GGCTTATTAC AAATCCTCTA AGAATATTAG ATTCAAAAGC TCCAGAAGAT CGTGAAATTT TATTGAATGT TCCTAATATT TCTTATTTTT ATAGTAAATC CTCTAATGAT TTTTTTGCAG AGGTTCTATA TGGGTTAGAT GAGCTCTGTA TTCCTTATAA AGTAAATCAT AGTATAGTTA GAGGTTTAGA TTATTATTGT AATACGGTGT TTGAGTTTAC AACGTCTAAA TTAGGAGCAC AAAATGCAGT TGTTGCTGGT GGTAGATATG ATGGATTAGT GAAGTCTATG GGAGGAAATG ATACTCCTGC TGTTGGTTTT GCTATGGGTG TTGAAAGAGT TAGTGCGTTG ATAGATTATA AACATCAAGA ACCTAGGAGT GTAGTATTGA TACCTATAGG GAAAGATGCA GTATCCTATG CTTTGAAGCT TGCTTATGAG TTACGTTGTA ATGGTATTTC TGTAAACTGG AATTATAAAA ATGCTAATTT GAGAAATGCA TTAAGGAAAG TAGGTGATGA TAATATAGTG TTAATTTTTG GAGATGAAGA GCTAAAAAAT AATACTATAC AAGTCAAAGA TATGAAAACT GGTGAACAAC AAGAAATAGT TAGATGTGAT TTATTGGACA CATTGTGTAA CAAAATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for EhchA.00686.a.A1.PW26852
PURIFICATION DATe: 8/17/2010
CONCENTRATION: 21.46mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLGNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcagc gtgataagtt aagagaagta aggggtacca aggatctatt agggattgaa ctttataagt ttcaatatat acatcgttta tctcagtcaa tagcgcatag atatggattt atagctgttg atactccaat cattgaattt actgaagtgt ttactaagac tttaggtgat gattcagata ttgtaatgaa ggaaatgtat aattttcagg ataaaagtgg tgaaaatatt acattaaggc cagaatttac atctgctatt gtgagggtat taattaataa aaatttaatt attcctgtca aattattttc tagtggacca gtatttcgtt atgaacgtcc tcaaaagtgt cgtcaaagac aatttcatca agttaatttt gagttttttg gaagtgatct tcctcttgca gatattgaaa tgatagcatt aggctatcat attttggatg agttgaagtt gttagatgat ataacgttag agattaattt tctaggtgat aaggaaacta tgaactcata taagctcagt cttatagagt atttaaacaa atataagaaa gatttatcag aagatagtca gcgtaggctt attacaaatc ctctaagaat attagattca aaagctccag aagatcgtga aattttattg aatgttccta atatttctta tttttatagt aaatcctcta atgatttttt tgcagaggtt ctatatgggt tagatgagct ctgtattcct tataaagtaa atcatagtat agttagaggt ttagattatt attgtaatac ggtgtttgag tttacaacgt ctaaattagg agcacaaaat gcagttgttg ctggtggtag atatgatgga ttagtgaagt ctatgggagg aaatgatact cctgctgttg gttttgctat gggtgttgaa agagttagtg cgttgataga ttataaacat caagaaccta ggagtgtagt attgatacct atagggaaag atgcagtatc ctatgctttg aagcttgctt atgagttacg ttgtaatggt atttctgtaa actggaatta taaaaatgct aatttgggaa atgcattaag gaaagtaggt gatgataata tagtgttaat ttttggagat gaagagctaa aaaataatac tatacaagtc aaagatatga aaactggtga acaacaagaa atagttagat gtgatttatt ggacacattg tgtaaca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRDKLREV RGTKDLLGIE LYKFQYIHRL SQSIAHRYGF IAVDTPIIEF TEVFTKTLGD DSDIVMKEMY NFQDKSGENI TLRPEFTSAI VRVLINKNLI IPVKLFSSGP VFRYERPQKC RQRQFHQVNF EFFGSDLPLA DIEMIALGYH ILDELKLLDD ITLEINFLGD KETMNSYKLS LIEYLNKYKK DLSEDSQRRL ITNPLRILDS KAPEDREILL NVPNISYFYS KSSNDFFAEV LYGLDELCIP YKVNHSIVRG LDYYCNTVFE FTTSKLGAQN AVVAGGRYDG LVKSMGGNDT PAVGFAMGVE RVSALIDYKH QEPRSVVLIP IGKDAVSYAL KLAYELRCNG ISVNWNYKNA NLRNALRKVG DDNIVLIFGD EELKNNTIQV KDMKTGEQQE IVRCDLLDTL CNKI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGCGTGAT AAGTTAAGAG AAGTAAGGGG TACCAAGGAT CTATTAGGGA TTGAACTTTA TAAGTTTCAA TATATACATC GTTTATCTCA GTCAATAGCG CATAGATATG GATTTATAGC TGTTGATACT CCAATCATTG AATTTACTGA AGTGTTTACT AAGACTTTAG GTGATGATTC AGATATTGTA ATGAAGGAAA TGTATAATTT TCAGGATAAA AGTGGTGAAA ATATTACATT AAGGCCAGAA TTTACATCTG CTATTGTGAG GGTATTAATT AATAAAAATT TAATTATTCC TGTCAAATTA TTTTCTAGTG GACCAGTATT TCGTTATGAA CGTCCTCAAA AGTGTCGTCA AAGACAATTT CATCAAGTTA ATTTTGAGTT TTTTGGAAGT GATCTTCCTC TTGCAGATAT TGAAATGATA GCATTAGGCT ATCATATTTT GGATGAGTTG AAGTTGTTAG ATGATATAAC GTTAGAGATT AATTTTCTAG GTGATAAGGA AACTATGAAC TCATATAAGC TCAGTCTTAT AGAGTATTTA AACAAATATA AGAAAGATTT ATCAGAAGAT AGTCAGCGTA GGCTTATTAC AAATCCTCTA AGAATATTAG ATTCAAAAGC TCCAGAAGAT CGTGAAATTT TATTGAATGT TCCTAATATT TCTTATTTTT ATAGTAAATC CTCTAATGAT TTTTTTGCAG AGGTTCTATA TGGGTTAGAT GAGCTCTGTA TTCCTTATAA AGTAAATCAT AGTATAGTTA GAGGTTTAGA TTATTATTGT AATACGGTGT TTGAGTTTAC AACGTCTAAA TTAGGAGCAC AAAATGCAGT TGTTGCTGGT GGTAGATATG ATGGATTAGT GAAGTCTATG GGAGGAAATG ATACTCCTGC TGTTGGTTTT GCTATGGGTG TTGAAAGAGT TAGTGCGTTG ATAGATTATA AACATCAAGA ACCTAGGAGT GTAGTATTGA TACCTATAGG GAAAGATGCA GTATCCTATG CTTTGAAGCT TGCTTATGAG TTACGTTGTA ATGGTATTTC TGTAAACTGG AATTATAAAA ATGCTAATTT GAGAAATGCA TTAAGGAAAG TAGGTGATGA TAATATAGTG TTAATTTTTG GAGATGAAGA GCTAAAAAAT AATACTATAC AAGTCAAAGA TATGAAAACT GGTGAACAAC AAGAAATAGT TAGATGTGAT TTATTGGACA CATTGTGTAA CAAAATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA