EhchA.00530.a
phosphoribosylaminoimidazole-succinocarboxamide synthase
| CENTER ID: | EhchA.00530.a |
| ORGANISM: | Ehrlichia chaffeensis str. Arkansas |
| ASSOCIATED DISEASE: | Human monocytic ehrlichiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.00530.a.A1.GE26741 | Full length( EhchA.00530.a ) | 1 | 242 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3KRE
DEPOSITED: 11/18/2009
DETERMINATION: XRay
CLONE: EhchA.00530.a.A1.GE26741
PROTEIN: EhchA.00530.a.A1.PS00302
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130014 |
| PATRIC ID: | fig|205920.11.peg.244 |
| RefSeq: | YP_507111.1 |
| UniProt: | Q2GHH2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENKEKIYEG KAKIIFATLN PLEVIQHFKD EITAFNNKKA AIIHEKGILN NYISSFLMKK LIDKGIKTHF ISLLNQREQL VKKITIIPIE VVIRNLAAGN FSKRFQIADG TPFKSPIIEF YYKNDELSDP MVSEGHILSF QWLTNQELEK IKILSLKINN ILSELFFNVG IKLVDFKLEF GKLHNDEQSD LFLADEISPD TCRLWDISTN KRLDKDRYRL NLGNVIEGYR EVAHKLNAIP NL
NT Sequence
ATGGAAAATA AAGAAAAAAT CTATGAAGGA AAAGCAAAAA TAATATTTGC AACGTTAAAC CCTCTAGAAG TAATACAACA TTTCAAAGAT GAGATTACCG CTTTCAACAA TAAAAAAGCA GCTATTATCC ATGAAAAAGG TATATTGAAC AACTATATAA GCAGTTTCTT AATGAAAAAA CTTATAGATA AAGGTATCAA AACACATTTT ATATCTTTAT TAAATCAAAG AGAACAACTA GTAAAAAAAA TAACAATAAT CCCAATCGAG GTTGTAATAA GGAATTTAGC AGCTGGTAAT TTTAGTAAAA GATTTCAAAT AGCAGACGGT ACCCCTTTTA AATCTCCCAT CATTGAATTT TATTATAAAA ATGATGAGCT ATCTGACCCT ATGGTAAGTG AGGGACATAT TCTGAGCTTC CAATGGCTTA CAAATCAAGA GTTAGAGAAA ATAAAAATAC TATCTCTAAA GATCAACAAT ATCCTCAGTG AGCTTTTTTT CAACGTTGGA ATCAAACTAG TAGATTTCAA ACTAGAATTT GGCAAATTGC ACAACGATGA ACAATCAGAT TTATTTTTAG CAGATGAAAT AAGCCCAGAT ACATGTAGAC TCTGGGACAT ATCTACTAAC AAAAGACTTG ACAAAGATCG TTACCGTTTG AACCTAGGAA ATGTAATAGA AGGATATAGA GAAGTAGCAC ATAAATTAAA TGCCATACCA AATCTT
Details for EhchA.00530.a.A1.GE26741
| HARVESTED ON: | 6/1/2009 |
| SEQUENCED ON: | 3/27/2012 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMENKEKIYE GKAKIIFATL NPLEVIQHFK DEITAFNNKK AAIIHEKGIL NNYISSFLMK KLIDKGIKTH FISLLNQREQ LVKKITIIPI EVVIRNLAAG NFSKRFQIAD GTPFKSPIIE FYYKNDELSD PMVSEGHILS FQWLTNQELE KIKILSLKIN NILSELFFNV GIKLVDFKLE FGKLHNDEQS DLFLADEISP DTCRLWDIST NKRLDKDRYR LNLGNVIEGY REVAHKLNAI P
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaaa ataaagaaaa aatctatgaa ggaaaagcaa aaataatatt tgcaacgtta aaccctctag aagtaataca acatttcaaa gatgagatta ccgctttcaa caataaaaaa gcagctatta tccatgaaaa aggtatattg aacaactata taagcagttt cttaatgaaa aaacttatag ataaaggtat caaaacacat tttatatctt tattaaatca aagagaacaa ctagtaaaaa aaataacaat aatcccaatc gaggttgtaa taaggaattt agcagctggt aattttagta aaagatttca aatagcagac ggtacccctt ttaaatctcc catcattgaa ttttattata aaaatgatga gctatctgac cctatggtaa gtgagggaca tattctgagc ttccaatggc ttacaaatca agagttagag aaaataaaaa tactatctct aaagatcaac aatatcctca gtgagctttt tttcaacgtt ggaatcaaac tagtagattt caaactagaa tttggcaaat tgcacaacga tgaacaatca gatttatttt tagcagatga aataagccca gatacatgta gactctggga catatctact aacaaaagac ttgacaaaga tcgttaccgt ttgaacctag gaaatgtaat agaaggatat agagaagtag cacataaatt aaatgccata ccaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMENKEKIYE GKAKIIFATL NPLEVIQHFK DEITAFNNKK AAIIHEKGIL NNYISSFLMK KLIDKGIKTH FISLLNQREQ LVKKITIIPI EVVIRNLAAG NFSKRFQIAD GTPFKSPIIE FYYKNDELSD PMVSEGHILS FQWLTNQELE KIKILSLKIN NILSELFFNV GIKLVDFKLE FGKLHNDEQS DLFLADEISP DTCRLWDIST NKRLDKDRYR LNLGNVIEGY REVAHKLNAI PNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaaataaa gaaaaaatct atgaaggaaa agcaaaaata atatttgcaa cgttaaaccc tctagaagta atacaacatt tcaaagatga gattaccgct ttcaacaata aaaaagcagc tattatccat gaaaaaggta tattgaacaa ctatataagc agtttcttaa tgaaaaaact tatagataaa ggtatcaaaa cacattttat atctttatta aatcaaagag aacaactagt aaaaaaaata acaataatcc caatcgaggt tgtaataagg aatttagcag ctggtaattt tagtaaaaga tttcaaatag cagacggtac cccttttaaa tctcccatca ttgaatttta ttataaaaat gatgagctat ctgaccctat ggtaagtgag ggacatattc tgagcttcca atggcttaca aatcaagagt tagagaaaat aaaaatacta tctctaaaga tcaacaatat cctcagtgag ctttttttca acgttggaat caaactagta gatttcaaac tagaatttgg caaattgcac aacgatgaac aatcagattt atttttagca gatgaaataa gcccagatac atgtagactc tgggacatat ctactaacaa aagacttgac aaagatcgtt accgtttgaa cctaggaaat gtaatagaag gatatagaga agtagcacat aaattaaatg ccataccaaa tcttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa