SSGCID: Target Page

EhchA.00528.a

Ferredoxin--NADP reductase

CENTER ID:	EhchA.00528.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00528.a.A1.GE30837	Full length( EhchA.00528.a )		1	338

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00528.a.A1.PW30725	Full length( EhchA.00528.a )		1	338

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.566
RefSeq:	YP_507458.1
UniProt:	Q2GGH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDYVTDIAV IGAGPVGIFT VFQAGMLKMR CCVIDALSEI GGQCLALYPE KPIYDIPGYP VINGKELIDS LKKQSEPFNP QYLLGQVAEK IEDYSDYFLI RTTTGIVVQS KVIIIAAGAG AFGPNRLPID NILDYENKSV FYQVRKVSDF CDKNIMIAGG GDSAADWAVE LSKVAKQLYV VHRRKNFRCA PNTALQMDNL SQSGKIKIIV PYQVKKLCGE NGKLHSVIVK NITNHEEMAL QVDYLFPFFG TSANLGPILN WGMEVKNYQI LVNAETCLTN RNRIYAVGDI ATYPGKLKLI LTGFSEAAMA CHHIYHVIYP NSPLNFQYST SKGIPENC

NT Sequence

ATGACAGATT ATGTAACTGA TATTGCGGTA ATAGGAGCTG GTCCTGTTGG GATATTTACT GTATTTCAGG CTGGTATGTT GAAAATGCGA TGTTGTGTTA TTGATGCATT AAGTGAAATT GGTGGGCAAT GTCTTGCATT GTATCCAGAG AAACCGATAT ATGATATTCC TGGATATCCA GTAATTAATG GTAAGGAATT AATAGATAGT TTAAAAAAGC AGTCTGAGCC TTTTAATCCT CAATATTTGT TAGGACAAGT TGCTGAAAAA ATAGAAGATT ACTCAGATTA TTTTTTGATA AGAACTACAA CAGGAATTGT AGTACAAAGT AAAGTTATTA TCATTGCTGC TGGAGCTGGA GCATTTGGTC CAAATCGTCT TCCTATAGAT AATATTCTTG ATTATGAGAA TAAATCAGTA TTTTACCAAG TGAGAAAGGT TTCAGATTTT TGTGATAAAA ATATTATGAT AGCAGGAGGA GGTGACTCAG CTGCTGATTG GGCAGTTGAG CTTTCTAAGG TTGCTAAACA GTTATATGTA GTACATAGAA GGAAAAATTT TCGTTGTGCT CCTAATACTG CATTGCAGAT GGATAATTTA TCACAGAGTG GAAAAATAAA GATTATTGTT CCATATCAAG TTAAAAAATT ATGTGGTGAA AATGGTAAAC TGCATTCTGT AATTGTTAAG AATATTACGA ATCATGAAGA AATGGCGCTA CAAGTTGATT ATTTATTTCC ATTTTTTGGT ACATCTGCAA ATCTTGGTCC TATATTGAAT TGGGGAATGG AAGTAAAAAA CTATCAAATT CTTGTTAATG CTGAGACTTG TCTAACAAAT CGCAATAGAA TATATGCAGT TGGTGATATA GCTACATATC CAGGAAAACT TAAGTTAATA CTTACAGGAT TTTCAGAGGC TGCAATGGCA TGTCATCATA TATATCATGT AATATACCCT AATTCTCCGT TAAATTTTCA ATATTCTACT TCAAAAGGTA TACCAGAAAA TTGT

Details for EhchA.00528.a.A1.GE30837

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MTDYVTDIAV IGAGPVGIFT VFQAGMLKMX CCVIDALSEI GGQCLALYPE KPIYDIPGYP VINGKELIDS LKKQSEPFNP QYLLGQVAEK IEDYSDYFLI RTTTGIVVQS KVIIIAAGAG AFGPNRLPID NILDYENKSV FYQVRKVSDF CDKNIMIAGG GDSAADWAVE LSKVAKQLYV VHRRKNFRCA PNTALQMDNL SQSGKIKIIV PYQVKKLCGE NGKLHSVIVK NITNHEEMAL QVDYLFPFFG TSANLGPILN WGMEVKNYQI LVNAETCLTN RNRIYAVGDI ATYPGKLKLI LTGFSEAAMA CHHIYHVIYP NSPLNFQYST SKGIPE

Validated NT Sequence

tttctggtat accttttgaa gtagaatatt gaaaatttaa cggagaatta gggtatatta catgatatat atgatgacat gccattgcag cctctgaaaa tcctgtaagt attaacttaa gttttcctgg atatgtagct atatcaccaa ctgcatatat tctattgcga tttgttagac aagtctcagc attaacaaga atttgatagt tttttacttc cattccccaa ttcaatatag gaccaagatt tgcagatgta ccaaaaaatg gaaataaata atcaacttgt agcgccattt cttcatgatt cgtaatattc ttaacaatta cagaatgcag tttaccattt tcaccacata attttttaac ttgatatgga acaataatct ttatttttcc actctgtgat aaattatcca tctgcaatgc agtattagga gcacaacgaa aatttttcct tctatgtact acatataact gtttagcaac cttagaaagc tcaactgccc aatcagcagc tgagtcacct cctcctgcta tcataatatt tttatcacaa aaatctgaaa cctttctcac ttggtaaaat actgatttat tctcataatc aagaatatta tctataggaa gacgatttgg accaaatgct ccagctccag cagcaatgat aataacttta ctttgtacta caattcctgt tgtagttctt atcaaaaaat aatctgagta atcttctatt ttttcagcaa cttgtcctaa caaatattga ggattaaaag gctcagactg cttttttaaa ctatctatta attccttacc attaattact ggatatccag gaatatcata tatcggtttc tctggataca atgcaagaca ttgcccacca atttcactta atgcatcaat aacacaacat ngcattttca acataccagc ctgaaataca gtaaatatcc caacaggacc agctcctatt accgcaatat cagttacata atctgtcatc gaaccaggac cctgggtctg agcttccagg gta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTDYVTDIA VIGAGPVGIF TVFQAGMLKM RCCVIDALSE IGGQCLALYP EKPIYDIPGY PVINGKELID SLKKQSEPFN PQYLLGQVAE KIEDYSDYFL IRTTTGIVVQ SKVIIIAAGA GAFGPNRLPI DNILDYENKS VFYQVRKVSD FCDKNIMIAG GGDSAADWAV ELSKVAKQLY VVHRRKNFRC APNTALQMDN LSQSGKIKII VPYQVKKLCG ENGKLHSVIV KNITNHEEMA LQVDYLFPFF GTSANLGPIL NWGMEVKNYQ ILVNAETCLT NRNRIYAVGD IATYPGKLKL ILTGFSEAAM ACHHIYHVIY PNSPLNFQYS TSKGIPENC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagattat gtaactgata ttgcggtaat aggagctggt cctgttggga tatttactgt atttcaggct ggtatgttga aaatgcgatg ttgtgttatt gatgcattaa gtgaaattgg tgggcaatgt cttgcattgt atccagagaa accgatatat gatattcctg gatatccagt aattaatggt aaggaattaa tagatagttt aaaaaagcag tctgagcctt ttaatcctca atatttgtta ggacaagttg ctgaaaaaat agaagattac tcagattatt ttttgataag aactacaaca ggaattgtag tacaaagtaa agttattatc attgctgctg gagctggagc atttggtcca aatcgtcttc ctatagataa tattcttgat tatgagaata aatcagtatt ttaccaagtg agaaaggttt cagatttttg tgataaaaat attatgatag caggaggagg tgactcagct gctgattggg cagttgagct ttctaaggtt gctaaacagt tatatgtagt acatagaagg aaaaattttc gttgtgctcc taatactgca ttgcagatgg ataatttatc acagagtgga aaaataaaga ttattgttcc atatcaagtt aaaaaattat gtggtgaaaa tggtaaactg cattctgtaa ttgttaagaa tattacgaat catgaagaaa tggcgctaca agttgattat ttatttccat tttttggtac atctgcaaat cttggtccta tattgaattg gggaatggaa gtaaaaaact atcaaattct tgttaatgct gagacttgtc taacaaatcg caatagaata tatgcagttg gtgatatagc tacatatcca ggaaaactta agttaatact tacaggattt tcagaggctg caatggcatg tcatcatata tatcatgtaa tataccctaa ttctccgtta aattttcaat attctacttc aaaaggtata ccagaaaatt gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for EhchA.00528.a.A1.PW30725

PURIFICATION DATe:	3/11/2011
CONCENTRATION:	5.93mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	1000µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGATTAT GTAACTGATA TTGCGGTAAT AGGAGCTGGT CCTGTTGGGA TATTTACTGT ATTTCAGGCT GGTATGTTGA AAATGCGATG TTGTGTTATT GATGCATTAA GTGAAATTGG TGGGCAATGT CTTGCATTGT ATCCAGAGAA ACCGATATAT GATATTCCTG GATATCCAGT AATTAATGGT AAGGAATTAA TAGATAGTTT AAAAAAGCAG TCTGAGCCTT TTAATCCTCA ATATTTGTTA GGACAAGTTG CTGAAAAAAT AGAAGATTAC TCAGATTATT TTTTGATAAG AACTACAACA GGAATTGTAG TACAAAGTAA AGTTATTATC ATTGCTGCTG GAGCTGGAGC ATTTGGTCCA AATCGTCTTC CTATAGATAA TATTCTTGAT TATGAGAATA AATCAGTATT TTACCAAGTG AGAAAGGTTT CAGATTTTTG TGATAAAAAT ATTATGATAG CAGGAGGAGG TGACTCAGCT GCTGATTGGG CAGTTGAGCT TTCTAAGGTT GCTAAACAGT TATATGTAGT ACATAGAAGG AAAAATTTTC GTTGTGCTCC TAATACTGCA TTGCAGATGG ATAATTTATC ACAGAGTGGA AAAATAAAGA TTATTGTTCC ATATCAAGTT AAAAAATTAT GTGGTGAAAA TGGTAAACTG CATTCTGTAA TTGTTAAGAA TATTACGAAT CATGAAGAAA TGGCGCTACA AGTTGATTAT TTATTTCCAT TTTTTGGTAC ATCTGCAAAT CTTGGTCCTA TATTGAATTG GGGAATGGAA GTAAAAAACT ATCAAATTCT TGTTAATGCT GAGACTTGTC TAACAAATCG CAATAGAATA TATGCAGTTG GTGATATAGC TACATATCCA GGAAAACTTA AGTTAATACT TACAGGATTT TCAGAGGCTG CAATGGCATG TCATCATATA TATCATGTAA TATACCCTAA TTCTCCGTTA AATTTTCAAT ATTCTACTTC AAAAGGTATA CCAGAAAATT GTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA