EhchA.00493.a
Immunogenic protein
| CENTER ID: | EhchA.00493.a |
| ORGANISM: | Ehrlichia chaffeensis str. Arkansas |
| ASSOCIATED DISEASE: | Human monocytic ehrlichiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.00493.a.A2.GE30836 | Mature protein( EhchA.00493.a ) | 21 | 325 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.00493.a.A2.PS01157 | Mature protein( EhchA.00493.a ) | 21 | 325 |
Structures
4DDD
DEPOSITED: 1/18/2012
DETERMINATION: XRay
CLONE: EhchA.00493.a.A2.GE30836
PROTEIN: EhchA.00493.a.A2.PS01157
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_144470 |
| PATRIC ID: | fig|205920.11.peg.563 |
| RefSeq: | YP_507455.1 |
| UniProt: | Q2GGH8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKVLIVYVF IISVLFNNAI ASSDDSLNRE YILIGTGSMT GVYYPIGGSI CRFIASDYGK DNKIICSISS TTGSVYNLNS IRYSNMDISI VQSDLEYYAY NGLGFYEKML PMDNLRMLAS LHKEYLTIVV KKSSNISVID DIKGKRVNIG SPGTGVRVAM LKLLGEKGWT KKDFSVMAEL KSSEQAQALC DNKIDVMVDV IGHPNASIQE ASATCDIKFI PLDDRLIDDL HAKYPYYQKD IISGGLYNDS PDIQTVSVKA SLVTTTELSN DLAYKIVKSI ATHLRELRSI TGALKTLTVQ DMAKSSITPM HDGAERYYKE IGAIK
NT Sequence
ATGAAAAAAG TGTTAATTGT GTATGTATTT ATTATTAGTG TACTTTTTAA TAATGCAATT GCCTCGTCAG ATGATAGTTT AAATAGAGAA TATATTTTAA TTGGTACTGG TTCTATGACA GGGGTATATT ACCCTATAGG AGGTAGTATT TGTAGATTTA TAGCAAGTGA TTATGGTAAG GATAATAAGA TTATTTGTTC TATTTCTTCT ACTACTGGAA GTGTATATAA CCTGAATTCT ATACGTTATT CGAATATGGA TATAAGTATT GTTCAGTCAG ATTTAGAGTA CTATGCGTAT AATGGTTTAG GGTTTTATGA AAAAATGCTT CCGATGGATA ATTTGAGAAT GTTAGCATCT CTGCATAAAG AGTATCTTAC TATTGTTGTT AAAAAAAGCT CCAATATATC AGTTATTGAT GATATAAAAG GCAAAAGAGT TAATATTGGT AGTCCTGGTA CTGGGGTAAG GGTAGCAATG TTAAAATTAT TAGGAGAAAA AGGTTGGACT AAGAAGGATT TTTCTGTCAT GGCAGAATTA AAATCATCAG AGCAAGCTCA AGCTTTATGT GATAATAAAA TTGATGTAAT GGTAGATGTG ATTGGACATC CTAATGCTTC AATTCAGGAA GCATCTGCAA CTTGTGATAT AAAATTTATT CCTTTAGATG ATAGATTAAT AGATGATTTA CATGCTAAGT ATCCCTATTA CCAAAAAGAT ATTATCAGTG GTGGGTTATA TAATGATTCA CCAGATATAC AGACTGTGTC AGTGAAGGCA TCGCTGGTTA CTACTACAGA GTTAAGTAAT GATTTAGCAT ATAAGATAGT AAAGTCTATA GCAACTCATT TACGAGAACT TCGTAGCATT ACTGGGGCTT TAAAAACTCT TACTGTGCAA GATATGGCAA AGTCAAGTAT TACTCCTATG CATGATGGTG CTGAACGTTA TTATAAAGAA ATTGGAGCTA TAAAA
Details for EhchA.00493.a.A2.GE30836
| HARVESTED ON: | 11/16/2010 |
| SEQUENCED ON: | 11/23/2010 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 87 |
Validated AA Sequence
MTGVYXPIGG SICRFIASDY GKDNKIICSI SSTTGSVYNL NSIRYSNMDI SIVQSDLEYY AYNGLGFYEK MLPMDNLRML ASLHKEYLTI VVKKSSNISV IDDIKGKRVN IGSPGTGVRV AMLKLLGEKG WTKKDFSVMA ELKSSEQAQA LCDNKIDVMV DVIGHPNASI QEASATCDIK FIPLDDRLID DLHAKYPYYQ KDIISGGLYN DSPDIQTVSV KASLVTTTEL SNDLAYKIVK SIATHLRELR SITGALKTLT VQDMAKSSIT PMHDGAERYY KEIGAIK
Validated NT Sequence
ttattatttt atagctccaa tttctttata ataacgttca gcaccatcat gcataggagt aatacttgac tttgccatat cttgcacagt aagagttttt aaagccccag taatgctacg aagttctcgt aaatgagttg ctatagactt tactatctta tatgctaaat cattacttaa ctctgtagta gtaaccagcg atgccttcac tgacacagtc tgtatatctg gtgaatcatt atataaccca ccactgataa tatctttttg gtaataggga tacttagcat gtaaatcatc tattaatcta tcatctaaag gaataaattt tatatcacaa gttgcagatg cttcctgaat tgaagcatta ggatgtccaa tcacatctac cattacatca attttattat cacataaagc ttgagcttgc tctgatgatt ttaattctgc catgacagaa aaatccttct tagtccaacc tttttctcct aataatttta acattgctac ccttacccca gtaccaggac taccaatatt aactcttttg ccttttatat catcaataac tgatatattg gagctttttt taacaacaat agtaagatac tctttatgca gagatgctaa cattctcaaa ttatccatcg gaagcatttt ttcataaaac cctaaaccat tatacgcata gtactctaaa tctgactgaa caatacttat atccatattc gaataacgta tagaattcag gttatataca cttccagtag tagaagaaat agaacaaata atcttattat ccttaccata atcacttgct ataaatctac aaatactacc tcctataggg naatataccc ctgtcataga accagtaccn attaaaatat attctctatt taaactatca tctgacga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASSDDSLN REYILIGTGS MTGVYYPIGG SICRFIASDY GKDNKIICSI SSTTGSVYNL NSIRYSNMDI SIVQSDLEYY AYNGLGFYEK MLPMDNLRML ASLHKEYLTI VVKKSSNISV IDDIKGKRVN IGSPGTGVRV AMLKLLGEKG WTKKDFSVMA ELKSSEQAQA LCDNKIDVMV DVIGHPNASI QEASATCDIK FIPLDDRLID DLHAKYPYYQ KDIISGGLYN DSPDIQTVSV KASLVTTTEL SNDLAYKIVK SIATHLRELR SITGALKTLT VQDMAKSSIT PMHDGAERYY KEIGAIK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcctcgtca gatgatagtt taaatagaga atatatttta attggtactg gttctatgac aggggtatat taccctatag gaggtagtat ttgtagattt atagcaagtg attatggtaa ggataataag attatttgtt ctatttcttc tactactgga agtgtatata acctgaattc tatacgttat tcgaatatgg atataagtat tgttcagtca gatttagagt actatgcgta taatggttta gggttttatg aaaaaatgct tccgatggat aatttgagaa tgttagcatc tctgcataaa gagtatctta ctattgttgt taaaaaaagc tccaatatat cagttattga tgatataaaa ggcaaaagag ttaatattgg tagtcctggt actggggtaa gggtagcaat gttaaaatta ttaggagaaa aaggttggac taagaaggat ttttctgtca tggcagaatt aaaatcatca gagcaagctc aagctttatg tgataataaa attgatgtaa tggtagatgt gattggacat cctaatgctt caattcagga agcatctgca acttgtgata taaaatttat tcctttagat gatagattaa tagatgattt acatgctaag tatccctatt accaaaaaga tattatcagt ggtgggttat ataatgattc accagatata cagactgtgt cagtgaaggc atcgctggtt actactacag agttaagtaa tgatttagca tataagatag taaagtctat agcaactcat ttacgagaac ttcgtagcat tactggggct ttaaaaactc ttactgtgca agatatggca aagtcaagta ttactcctat gcatgatggt gctgaacgtt attataaaga aattggagct ataaaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for EhchA.00493.a.A2.PS01157
| PURIFICATION DATe: | 7/20/2011 |
| CONCENTRATION: | 18.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 87 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTGVYXPIGG SICRFIASDY GKDNKIICSI SSTTGSVYNL NSIRYSNMDI SIVQSDLEYY AYNGLGFYEK MLPMDNLRML ASLHKEYLTI VVKKSSNISV IDDIKGKRVN IGSPGTGVRV AMLKLLGEKG WTKKDFSVMA ELKSSEQAQA LCDNKIDVMV DVIGHPNASI QEASATCDIK FIPLDDRLID DLHAKYPYYQ KDIISGGLYN DSPDIQTVSV KASLVTTTEL SNDLAYKIVK SIATHLRELR SITGALKTLT VQDMAKSSIT PMHDGAERYY KEIGAIK
Validated NT Sequence
ttattatttt atagctccaa tttctttata ataacgttca gcaccatcat gcataggagt aatacttgac tttgccatat cttgcacagt aagagttttt aaagccccag taatgctacg aagttctcgt aaatgagttg ctatagactt tactatctta tatgctaaat cattacttaa ctctgtagta gtaaccagcg atgccttcac tgacacagtc tgtatatctg gtgaatcatt atataaccca ccactgataa tatctttttg gtaataggga tacttagcat gtaaatcatc tattaatcta tcatctaaag gaataaattt tatatcacaa gttgcagatg cttcctgaat tgaagcatta ggatgtccaa tcacatctac cattacatca attttattat cacataaagc ttgagcttgc tctgatgatt ttaattctgc catgacagaa aaatccttct tagtccaacc tttttctcct aataatttta acattgctac ccttacccca gtaccaggac taccaatatt aactcttttg ccttttatat catcaataac tgatatattg gagctttttt taacaacaat agtaagatac tctttatgca gagatgctaa cattctcaaa ttatccatcg gaagcatttt ttcataaaac cctaaaccat tatacgcata gtactctaaa tctgactgaa caatacttat atccatattc gaataacgta tagaattcag gttatataca cttccagtag tagaagaaat agaacaaata atcttattat ccttaccata atcacttgct ataaatctac aaatactacc tcctataggg naatataccc ctgtcataga accagtaccn attaaaatat attctctatt taaactatca tctgacga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASSDDSLN REYILIGTGS MTGVYYPIGG SICRFIASDY GKDNKIICSI SSTTGSVYNL NSIRYSNMDI SIVQSDLEYY AYNGLGFYEK MLPMDNLRML ASLHKEYLTI VVKKSSNISV IDDIKGKRVN IGSPGTGVRV AMLKLLGEKG WTKKDFSVMA ELKSSEQAQA LCDNKIDVMV DVIGHPNASI QEASATCDIK FIPLDDRLID DLHAKYPYYQ KDIISGGLYN DSPDIQTVSV KASLVTTTEL SNDLAYKIVK SIATHLRELR SITGALKTLT VQDMAKSSIT PMHDGAERYY KEIGAIK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCTCGTCA GATGATAGTT TAAATAGAGA ATATATTTTA ATTGGTACTG GTTCTATGAC AGGGGTATAT TACCCTATAG GAGGTAGTAT TTGTAGATTT ATAGCAAGTG ATTATGGTAA GGATAATAAG ATTATTTGTT CTATTTCTTC TACTACTGGA AGTGTATATA ACCTGAATTC TATACGTTAT TCGAATATGG ATATAAGTAT TGTTCAGTCA GATTTAGAGT ACTATGCGTA TAATGGTTTA GGGTTTTATG AAAAAATGCT TCCGATGGAT AATTTGAGAA TGTTAGCATC TCTGCATAAA GAGTATCTTA CTATTGTTGT TAAAAAAAGC TCCAATATAT CAGTTATTGA TGATATAAAA GGCAAAAGAG TTAATATTGG TAGTCCTGGT ACTGGGGTAA GGGTAGCAAT GTTAAAATTA TTAGGAGAAA AAGGTTGGAC TAAGAAGGAT TTTTCTGTCA TGGCAGAATT AAAATCATCA GAGCAAGCTC AAGCTTTATG TGATAATAAA ATTGATGTAA TGGTAGATGT GATTGGACAT CCTAATGCTT CAATTCAGGA AGCATCTGCA ACTTGTGATA TAAAATTTAT TCCTTTAGAT GATAGATTAA TAGATGATTT ACATGCTAAG TATCCCTATT ACCAAAAAGA TATTATCAGT GGTGGGTTAT ATAATGATTC ACCAGATATA CAGACTGTGT CAGTGAAGGC ATCGCTGGTT ACTACTACAG AGTTAAGTAA TGATTTAGCA TATAAGATAG TAAAGTCTAT AGCAACTCAT TTACGAGAAC TTCGTAGCAT TACTGGGGCT TTAAAAACTC TTACTGTGCA AGATATGGCA AAGTCAAGTA TTACTCCTAT GCATGATGGT GCTGAACGTT ATTATAAAGA AATTGGAGCT ATAAAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA