SSGCID: Target Page

EhchA.00426.a

NADH dehydrogenase subunit B

CENTER ID:	EhchA.00426.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00426.a.A1.GE26734	Full length( EhchA.00426.a )		1	179

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.685
RefSeq:	YP_507585.1
UniProt:	Q2GG48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MINKNDSVLN NQFWQSYNHQ GFMVTQFSDL INYISNWARS NSLWPMTFGL ACCAVEMMHT AASRYDLDRY GVMFRASPRQ ADVMIVAGTL TNKMAPALRK VYDQMTEPRY VISMGSCANG GGYYHYSYSV VRGCDRIVPV DIYVPGCPPT AEALLYGIFC LQQKINRGNT SITRKSTQD

NT Sequence

ATGATAAACA AGAATGATTC TGTATTAAAT AATCAATTCT GGCAAAGTTA CAACCATCAA GGTTTCATGG TAACTCAGTT TTCTGACTTA ATCAACTACA TATCAAATTG GGCAAGATCA AATTCTTTAT GGCCTATGAC ATTTGGATTA GCTTGTTGTG CTGTAGAAAT GATGCACACT GCAGCAAGCA GATATGACCT AGACAGATAT GGTGTTATGT TTCGTGCAAG CCCCAGACAA GCAGATGTTA TGATCGTTGC TGGAACACTC ACCAACAAAA TGGCACCTGC ACTACGTAAA GTATACGACC AAATGACAGA GCCACGTTAC GTCATTTCAA TGGGAAGCTG TGCGAATGGT GGCGGATATT ATCATTATTC ATATTCAGTA GTTAGAGGAT GTGACCGCAT AGTACCAGTA GATATTTATG TTCCAGGGTG CCCACCAACA GCAGAAGCAT TATTATACGG AATATTTTGT CTTCAACAAA AAATCAATAG AGGGAATACT TCTATTACAA GAAAATCTAC ACAAGAC

Details for EhchA.00426.a.A1.GE26734

HARVESTED ON:	6/8/2009
SEQUENCED ON:	7/1/2009
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMINKNDSVL NNQFWQSYNH QGFMVTQFSD LINYISNWAR SNSLWPMTFG LACCAVEMMH TSASRYDLDR YGVMFRASPR QADVMIVAGT LTNKMAPALR KVYDQMTEPR YVISMGSCAN GGGYYHYSYS VVRGCDRIVP VDIYVPGCPP TAEALLYGIF CLQQKINRGN TSITRKS

Validated NT Sequence

tgtagatttt cttgtaatag aagtattccc tctattgatt ttttgttgaa gacaaaatat tccgtataat aatgcttctg ctgttggtgg gcaccctgga acataaatat ctactggtac tatgcggtca catcctctaa ctactgaata tgaataatga taatatccgc caccattcgc acagcttccc attgaaatga cgtaacgtgg ctctgtcatt tggtcgtata ctttacgtag tgcaggtgcc attttgttgg tgagtgttcc agcaacgatc ataacatctg cttgtctggg gcttgcacga aacataacac catatctgtc taggtcatat ctgcttgctg aagtgtgcat catttctaca gcacaacaag ctaatccaaa tgtcataggc cataaagaat ttgatcttgc ccaatttgat atgtagttga ttaagtcaga aaactgagtt accatgaaac cttgatggtt gtaactttgc cagaattgat tatttaatac agaatcattc ttgtttatca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMINKNDSVL NNQFWQSYNH QGFMVTQFSD LINYISNWAR SNSLWPMTFG LACCAVEMMH TAASRYDLDR YGVMFRASPR QADVMIVAGT LTNKMAPALR KVYDQMTEPR YVISMGSCAN GGGYYHYSYS VVRGCDRIVP VDIYVPGCPP TAEALLYGIF CLQQKINRGN TSITRKSTQD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataaacaag aatgattctg tattaaataa tcaattctgg caaagttaca accatcaagg tttcatggta actcagtttt ctgacttaat caactacata tcaaattggg caagatcaaa ttctttatgg cctatgacat ttggattagc ttgttgtgct gtagaaatga tgcacactgc agcaagcaga tatgacctag acagatatgg tgttatgttt cgtgcaagcc ccagacaagc agatgttatg atcgttgctg gaacactcac caacaaaatg gcacctgcac tacgtaaagt atacgaccaa atgacagagc cacgttacgt catttcaatg ggaagctgtg cgaatggtgg cggatattat cattattcat attcagtagt tagaggatgt gaccgcatag taccagtaga tatttatgtt ccagggtgcc caccaacagc agaagcatta ttatacggaa tattttgtct tcaacaaaaa atcaatagag ggaatacttc tattacaaga aaatctacac aagacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa