EhchA.00418.a

succinyl-CoA synthetase, beta subunit

CENTER ID: EhchA.00418.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00418.a.A1.GE26733 Full length( EhchA.00418.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.844
RefSeq: YP_507766.1
UniProt: Q2GFL7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIHEYQAKH ILSKFGVNVP KGVVVHSLGE VDGALSQLNS KVVVVKAQIH AGGRGKAGGV VVSRTLDETK TSIKNMLGST LITHQTSKDG QKVRKVYLEE GCNIKKEYYI SAIVNRKQGQ VSIIFSTEGG VDIEEVAATS PEKVIVCNIN PVFGFQGFHG RNLCFDSNLS LDQTRKISDI AGKIYKAVLS TDANQIEINP LVETSSGEFI ALDAKINFDD NALYRHPDIQ ELRDYDEEIK EEIEASKHGL SYIKMDGNIG CMVNGAGLAM ATMDIIKYYG AEPANFLDVG GGASQKTVTE AFKIILADDK VNGILVNIFG GIMRCDIIAN GIIAAIQEIG INVPLVVRLS GTNFELGKKL LDDSNLNIIT ANDLSEAAYN IVNIVRK
NT Sequence
ATGAATATTC ATGAGTATCA GGCAAAGCAT ATACTGAGCA AGTTTGGTGT AAACGTTCCG AAAGGGGTTG TAGTACATTC ATTAGGCGAA GTTGATGGTG CTCTCAGTCA GTTAAATTCA AAGGTAGTCG TTGTAAAAGC TCAAATCCAT GCTGGTGGTA GAGGAAAAGC TGGAGGAGTT GTAGTATCTC GTACATTAGA TGAAACCAAA ACGTCTATAA AGAATATGTT AGGATCAACT CTGATTACTC ATCAGACCTC AAAAGATGGA CAGAAAGTAA GAAAAGTATA TTTAGAAGAA GGATGCAACA TCAAGAAAGA ATATTATATA AGTGCCATTG TAAATCGTAA ACAAGGTCAG GTAAGTATAA TATTTTCAAC TGAAGGTGGT GTTGACATAG AAGAAGTAGC TGCAACTTCT CCAGAAAAAG TTATTGTATG CAACATCAAC CCAGTATTTG GATTCCAAGG TTTTCATGGA CGCAATTTAT GCTTTGACAG TAATTTAAGT TTAGATCAAA CAAGAAAAAT TTCTGATATA GCTGGGAAAA TATATAAAGC TGTGTTAAGC ACTGATGCTA ACCAGATAGA AATTAATCCT CTAGTAGAAA CATCATCAGG GGAATTCATT GCTCTTGATG CAAAAATAAA CTTCGATGAC AATGCACTAT ATCGTCATCC TGATATTCAA GAATTACGTG ACTATGATGA AGAGATCAAA GAAGAAATAG AAGCTTCAAA ACATGGTTTA AGCTATATAA AGATGGATGG TAATATTGGA TGTATGGTAA ATGGTGCAGG CCTTGCTATG GCAACTATGG ACATTATAAA ATATTACGGT GCTGAACCTG CAAATTTCTT AGATGTAGGT GGAGGAGCAA GTCAAAAAAC TGTTACTGAA GCATTCAAGA TTATTCTTGC AGATGATAAG GTTAATGGGA TACTAGTTAA CATATTTGGT GGTATAATGC GTTGTGATAT AATTGCCAAC GGTATTATTG CTGCCATACA AGAAATTGGT ATAAATGTTC CACTAGTTGT AAGGTTATCT GGAACAAATT TTGAATTAGG AAAAAAGTTA CTAGATGATT CAAATCTAAA TATTATTACA GCAAACGATT TAAGTGAAGC AGCATACAAT ATAGTTAATA TAGTAAGAAA A
Details for EhchA.00418.a.A1.GE26733
HARVESTED ON: 6/8/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MNIHEYQAKH ILSKFGVNVP KGVVVHSLGE VDGALSQLNS KVVVVKAQIH AGGRGKAGGV VVSRTLDETK TSIKNMLGST LITHQTSKDG QKVRKVYLEE GCNIKKEYYI SAIVNRKQGQ VSIIFSTEGG VDIEEVAATS PEKVIVCNIN PVFGFQGFHG RNLCFDSNLS LDQTRKISDI AGKIYKAVLS TDANQIEINP LVETSSGEFI ALDAKINFDD NALYRHPDIQ ELRDYDEEIK EEIEASKHGL SYIKMDGNIG CMVNGAGLAM ATMDIIKYYG AEPANFLDVG GGASQKTVTE AFKIILADDK VNGILVNIFG GIMRCDIIAN GIIAAIQEIG INVPLVVRLS GTNFELGKKL LXDSNLNIIT ANDLSEAAYN IVNIVRK
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatgaatat tcatgagtat caggcaaagc atatactgag caagtttggt gtaaacgttc cgaaaggggt tgtagtacat tcattaggcg aagttgatgg tgctctcagt cagttaaatt caaaggtagt cgttgtaaaa gctcaaatcc atgctggtgg tagaggaaaa gctggaggag ttgtagtatc tcgtacatta gatgaaacca aaacgtctat aaagaatatg ttaggatcaa ctctgattac tcatcagacc tcaaaagatg gacagaaagt aagaaaagta tatttagaag aaggatgcaa catcaagaaa gaatattata taagtgccat tgtaaatcgt aaacaaggtc aggtaagtat aatattttca actgaaggtg gtgttgacat agaagaagta gctgcaactt ctccagaaaa agttattgta tgcaacatca acccagtatt tggattccaa ggttttcatg gacgcaattt atgctttgac agtaatttaa gtttagatca aacaagaaaa atttctgata tagctgggaa aatatataaa gctgtgttaa gcactgatgc taaccagata gaaattaatc ctctagtaga aacatcatca ggggaattca ttgctcttga tgcaaaaata aacttcgatg acaatgcact atatcgtcat cctgatattc aagaattacg tgactatgat gaagagatca aagaagaaat agaagcttca aaacatggtt taagctatat aaagatggat ggtaatattg gatgtatggt aaatggtgca ggccttgcta tggcaactat ggacattata aaatattacg gtgctgaacc tgcaaatttc ttagatgtag gtggaggagc aagtcaaaaa actgttactg aagcattcaa gattattctt gcagatgata aggttaatgg gatactagtt aacatatttg gtggtataat gcgttgtgat ataattgcca acggtattat tgctgccata caagaaattg gtataaatgt tccactagtt gtaaggttat ctggaacaaa ttttgaatta ggaaaaaagt tactanatga ttcaaatcta aatattatta cagcaaacga tttaagtgaa gcagcataca atatagttaa tatagtaaga aaataata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIHEYQAK HILSKFGVNV PKGVVVHSLG EVDGALSQLN SKVVVVKAQI HAGGRGKAGG VVVSRTLDET KTSIKNMLGS TLITHQTSKD GQKVRKVYLE EGCNIKKEYY ISAIVNRKQG QVSIIFSTEG GVDIEEVAAT SPEKVIVCNI NPVFGFQGFH GRNLCFDSNL SLDQTRKISD IAGKIYKAVL STDANQIEIN PLVETSSGEF IALDAKINFD DNALYRHPDI QELRDYDEEI KEEIEASKHG LSYIKMDGNI GCMVNGAGLA MATMDIIKYY GAEPANFLDV GGGASQKTVT EAFKIILADD KVNGILVNIF GGIMRCDIIA NGIIAAIQEI GINVPLVVRL SGTNFELGKK LLDDSNLNII TANDLSEAAY NIVNIVRK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatattcat gagtatcagg caaagcatat actgagcaag tttggtgtaa acgttccgaa aggggttgta gtacattcat taggcgaagt tgatggtgct ctcagtcagt taaattcaaa ggtagtcgtt gtaaaagctc aaatccatgc tggtggtaga ggaaaagctg gaggagttgt agtatctcgt acattagatg aaaccaaaac gtctataaag aatatgttag gatcaactct gattactcat cagacctcaa aagatggaca gaaagtaaga aaagtatatt tagaagaagg atgcaacatc aagaaagaat attatataag tgccattgta aatcgtaaac aaggtcaggt aagtataata ttttcaactg aaggtggtgt tgacatagaa gaagtagctg caacttctcc agaaaaagtt attgtatgca acatcaaccc agtatttgga ttccaaggtt ttcatggacg caatttatgc tttgacagta atttaagttt agatcaaaca agaaaaattt ctgatatagc tgggaaaata tataaagctg tgttaagcac tgatgctaac cagatagaaa ttaatcctct agtagaaaca tcatcagggg aattcattgc tcttgatgca aaaataaact tcgatgacaa tgcactatat cgtcatcctg atattcaaga attacgtgac tatgatgaag agatcaaaga agaaatagaa gcttcaaaac atggtttaag ctatataaag atggatggta atattggatg tatggtaaat ggtgcaggcc ttgctatggc aactatggac attataaaat attacggtgc tgaacctgca aatttcttag atgtaggtgg aggagcaagt caaaaaactg ttactgaagc attcaagatt attcttgcag atgataaggt taatgggata ctagttaaca tatttggtgg tataatgcgt tgtgatataa ttgccaacgg tattattgct gccatacaag aaattggtat aaatgttcca ctagttgtaa ggttatctgg aacaaatttt gaattaggaa aaaagttact agatgattca aatctaaata ttattacagc aaacgattta agtgaagcag catacaatat agttaatata gtaagaaaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a