EhchA.00374.a

fatty acid/phospholipid synthesis protein

CENTER ID: EhchA.00374.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00374.a.MB1.GE30412 Full length( EhchA.00374.a ) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.382
RefSeq: YP_507265.1
UniProt: Q2GH18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIISIAVDA MGGDFAPEAV VSGLDFALTN LLDDQNVSFN IYGQGSQVLP ILDKYKDLKE HSVFIDTPEV VLANDKPSFA LRKRRSSSMW CAIDSIKSGV TSGVVSSGNT GALMAISRFL LGTLPNIDRP AICTALPSRG EEYFVLLDLG ANIESSSNAL FQFAIMGSAF AKAVLNIASP KVALLNVGQE EVKGTDVIRE AFLLLKQAEG RINFCGYIEP IDILGDKVDV VVTDGFCGNI VLKVAESIAY TFKSVFEKSV TSSIISKFAG LLLKSQMKKD FMRFNPKMYN GAMLLGLNGV VVKSHGNADK VAFAHAIKVT VNAVRNDINA KIIHELS
NT Sequence
ATGAGCATAA TATCAATTGC AGTGGATGCT ATGGGTGGAG ATTTTGCTCC TGAAGCAGTA GTAAGCGGGT TGGATTTTGC TTTAACAAAT TTATTAGATG ACCAAAATGT TTCTTTTAAT ATATACGGGC AGGGAAGCCA AGTGTTGCCT ATATTAGATA AATATAAGGA TTTAAAAGAA CATAGTGTAT TTATTGATAC TCCAGAAGTA GTATTAGCAA ATGATAAACC ATCCTTTGCG CTAAGAAAAC GTCGATCTTC TAGTATGTGG TGTGCAATTG ATAGTATAAA AAGTGGTGTA ACCTCTGGAG TTGTATCTTC TGGTAACACA GGTGCTTTAA TGGCTATTTC ACGTTTTTTG TTGGGAACAC TTCCTAACAT AGATCGTCCA GCGATTTGTA CTGCTCTTCC TTCTAGAGGT GAGGAGTATT TTGTATTATT AGATTTGGGT GCAAATATTG AAAGCAGTTC AAATGCTTTA TTTCAATTTG CTATTATGGG GAGTGCTTTT GCAAAAGCTG TGTTGAATAT TGCTAGCCCT AAAGTAGCAT TACTAAATGT TGGCCAAGAA GAAGTTAAAG GCACAGATGT TATCAGAGAA GCATTTTTAT TATTGAAACA AGCTGAAGGT AGGATAAATT TTTGTGGGTA TATTGAACCT ATTGATATAC TAGGAGATAA AGTTGATGTT GTAGTTACTG ATGGATTTTG TGGGAATATT GTATTAAAGG TAGCTGAGTC TATTGCATAT ACTTTTAAGT CAGTTTTTGA AAAGTCCGTT ACTAGTTCTA TAATATCAAA ATTTGCAGGA TTATTGCTAA AATCCCAGAT GAAAAAAGAT TTTATGAGGT TTAATCCTAA AATGTATAAT GGGGCTATGT TATTAGGTCT AAATGGTGTT GTGGTGAAAA GTCATGGAAA TGCTGATAAG GTAGCTTTTG CTCATGCTAT TAAAGTGACT GTAAATGCTG TGCGTAATGA TATCAATGCT AAAATTATTC ATGAATTAAG T
Details for EhchA.00374.a.MB1.GE30412
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 81kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 37
Validated AA Sequence
MSIISIAVDA MGGDFAPEAV VSGLDFALTN LLDDQNVSFN IYGQGSQVLP ILDKYKDLKE HSVFIDTPEV VLANDKPSFA LRKRRSSSMW CAIDSIKSGV TSGVVSSGNT GALMAISRFL LGTLPNIDRP AICTALPSRG EEYFVLLDLG ANIESSSXAL FQFAIMGSAF AKAVLNXASP KVXXLNVGQE EXKGTDVIXE AFLLLKXAEX RINFCGYIEP XDILGDXVDV VVXDGFCGNI VLXXSXXYXI YFXSVFXKXX TSXIXXXFAX XXLXXXXXXX X
Validated NT Sequence
gnnctcagac ccagggtcct ggttcgatga gcataatatc aattgcagtg gatgctatgg gtggagattt tgctcctgaa gcagtagtaa gcgggttgga ttttgcttta acaaatttat tagatgacca aaatgtttct tttaatatat acgggcaggg aagccaagtg ttgcctatat tagataaata taaggattta aaagaacata gtgtatttat tgatactcca gaagtagtat tagcaaatga taaaccatcc tttgcgctaa gaaaacgtcg atcttctagt atgtggtgtg caattgatag tataaaaagt ggtgtaacct ctggagttgt atcttctggt aacacaggtg ctttaatggc tatttcacgt tttttgttgg gaacacttcc taacatagat cgtccagcga tttgtactgc tcttccttct agaggtgagg agtattttgt attattagat ttgggtgcna atattgaaag cagttcanat gctttatttc aatttgctat tatggggagt gcttttgcna aagctgtgtt gaatatngct agccctaaag tancantact aaatgttggn caagaagaan ttaaaggcac agatgttatc ngagaagcat ttttattatt gaaacangct gaangtagga taaatttttg tgggtatatt gaacctatng atatactagg agatanagtt gatgttgtag ttnctgatgg attttgtggg aatattgtat taaanngnag ctnannctat ngcatatact ttnagtcagt ttttgnnaag nnnnttacta gtnntatana tnnnnnnttt gcnnnnnnnn ngctnannnn cncngannaa aannattnnt gaggntnncn nnnatgtntn nngngnnnnt nnnnttnnnn nctannggnn nnnnnnnnna nnntnanann nnnnnntttn gnnnnntgcn ntaannnnnn nnnnnnnnnn nnnnnnnnnn ncannnnnnn nnnnnnnntn nannnnncnn nantnnnnnn nnnnnnnnnn nnnnnnnnnn nnncnanna
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSIISIAVDA MGGDFAPEAV VSGLDFALTN LLDDQNVSFN IYGQGSQVLP ILDKYKDLKE HSVFIDTPEV VLANDKPSFA LRKRRSSSMW CAIDSIKSGV TSGVVSSGNT GALMAISRFL LGTLPNIDRP AICTALPSRG EEYFVLLDLG ANIESSSNAL FQFAIMGSAF AKAVLNIASP KVALLNVGQE EVKGTDVIRE AFLLLKQAEG RINFCGYIEP IDILGDKVDV VVTDGFCGNI VLKVAESIAY TFKSVFEKSV TSSIISKFAG LLLKSQMKKD FMRFNPKMYN GAMLLGLNGV VVKSHGNADK VAFAHAIKVT VNAVRNDINA KIIHELS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgag cataatatca attgcagtgg atgctatggg tggagatttt gctcctgaag cagtagtaag cgggttggat tttgctttaa caaatttatt agatgaccaa aatgtttctt ttaatatata cgggcaggga agccaagtgt tgcctatatt agataaatat aaggatttaa aagaacatag tgtatttatt gatactccag aagtagtatt agcaaatgat aaaccatcct ttgcgctaag aaaacgtcga tcttctagta tgtggtgtgc aattgatagt ataaaaagtg gtgtaacctc tggagttgta tcttctggta acacaggtgc tttaatggct atttcacgtt ttttgttggg aacacttcct aacatagatc gtccagcgat ttgtactgct cttccttcta gaggtgagga gtattttgta ttattagatt tgggtgcaaa tattgaaagc agttcaaatg ctttatttca atttgctatt atggggagtg cttttgcaaa agctgtgttg aatattgcta gccctaaagt agcattacta aatgttggcc aagaagaagt taaaggcaca gatgttatca gagaagcatt tttattattg aaacaagctg aaggtaggat aaatttttgt gggtatattg aacctattga tatactagga gataaagttg atgttgtagt tactgatgga ttttgtggga atattgtatt aaaggtagct gagtctattg catatacttt taagtcagtt tttgaaaagt ccgttactag ttctataata tcaaaatttg caggattatt gctaaaatcc cagatgaaaa aagattttat gaggtttaat cctaaaatgt ataatggggc tatgttatta ggtctaaatg gtgttgtggt gaaaagtcat ggaaatgctg ataaggtagc ttttgctcat gctattaaag tgactgtaaa tgctgtgcgt aatgatatca atgctaaaat tattcatgaa ttaagttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa