EhchA.00369.a

D-alanyl-D-alanine carboxypeptidase family protein

CENTER ID: EhchA.00369.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EhchA.00369.a.A2.GE30835 Mature protein( EhchA.00369.a ) 25 381
EhchA.00369.a.AE2.GE43222 Mature protein( EhchA.00369.a ) 25 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EhchA.00369.a.A2.PW30723 Mature protein( EhchA.00369.a ) 25 381

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.917
RefSeq: YP_507852.1
UniProt: Q2GFD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRHIILLLL YCFISIQVHI SNAVALPIQT TAPQAAVFDF FSNTMLLEHN IDEQIAPSSM TNLMTLYVTF YYIKAGFVKM EDKFKTSKEA WQKGGNSIFL RAGQLVAVRD LINGIITTSA NDACITLAEG VAGSQENFVD EMNRIAQKLN LTKSHFSNVI GAQDKNQFMS IRDLITLTVR IFEDFPEYYH LFSKKDFKYN NIYQENINLL SPDNRIDGII SIYTDAGGYG SIVAAKHEGR RIFILISGLK TEKERISEIK QLLDYAFNDF SSQTIFHKGS KAKEIIVKNG DAKYVEAVFN NDVIILYPKG SYDTVKTFFS HENAISAPVK KGQEVGHLHI QVPELTERVI PMYAANDINH LNFFQRILYM FSPKTDKVAT S
NT Sequence
ATGTTACGCC ATATAATCCT TCTACTGTTA TATTGTTTTA TTAGTATTCA GGTACATATA AGTAATGCTG TGGCCTTACC TATACAAACA ACAGCACCTC AAGCAGCAGT ATTCGATTTC TTTTCTAATA CAATGTTATT AGAACACAAC ATTGACGAAC AAATTGCACC CTCGTCTATG ACTAACTTAA TGACCTTATA TGTTACATTT TACTACATAA AAGCTGGATT TGTAAAAATG GAGGATAAAT TTAAGACTAG TAAAGAGGCT TGGCAGAAAG GAGGTAACTC CATTTTCCTA AGGGCGGGAC AGCTAGTAGC AGTAAGAGAT TTAATTAATG GTATTATTAC AACATCTGCA AATGACGCCT GTATTACTCT TGCAGAAGGC GTAGCAGGTT CACAGGAGAA CTTTGTTGAT GAAATGAATC GTATAGCACA AAAACTTAAC CTAACAAAAT CTCATTTCAG CAATGTAATA GGTGCTCAAG ATAAAAATCA ATTCATGTCA ATACGCGACT TAATAACTCT TACAGTAAGA ATTTTCGAAG ATTTTCCTGA ATATTATCAT CTATTCTCCA AAAAAGATTT TAAGTACAAT AATATATACC AAGAAAACAT CAACTTATTG TCACCAGATA ATAGAATAGA TGGGATAATT AGCATATATA CAGATGCAGG AGGATACGGG TCTATAGTAG CTGCTAAACA TGAAGGCAGA CGTATCTTCA TATTAATCAG CGGTCTTAAA ACTGAAAAAG AACGCATATC TGAAATAAAG CAGTTGCTAG ATTATGCTTT TAATGATTTT AGCAGCCAAA CCATCTTTCA CAAAGGTAGT AAAGCCAAGG AAATAATAGT TAAAAACGGT GATGCAAAAT ATGTAGAAGC TGTGTTCAAT AACGATGTGA TTATTCTATA TCCTAAAGGC TCATATGATA CAGTCAAAAC CTTTTTTTCA CACGAAAATG CAATATCTGC ACCAGTAAAA AAAGGACAAG AAGTTGGTCA TCTTCACATA CAGGTACCAG AACTTACAGA ACGCGTTATA CCTATGTACG CAGCAAATGA TATAAACCAT CTCAATTTCT TCCAAAGAAT ATTGTACATG TTTTCCCCTA AAACAGATAA AGTTGCTACT TCA
Details for EhchA.00369.a.A2.GE30835
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Validated AA Sequence
MLLEHNIDEQ IAPSSMTNLM TLYVTFYYIK AGFVKMEDKF KTSKEAWQKG GNSIFLRAGQ LVAVRDLING IITTSANDAC ITLAEGVAGS QENFVDEMNR IAQKLNLTKS HFSNVIGAQD KNQFMSIRDL ITLTVRIFED FPEYYHLFSK KDFKYNNIYQ ENINLLSPDN RIDGIISIYT DAGGYGSIVA AKHEGRRIFI LISGLKTEKE RISEIKQLLD YAFNDFSSQT IFHKGSKAKE IIVKNGDAKY VEAVFNNDVI ILYPKGSYDT VKTFFSHENA ISAPVKKGQE VGHLHIQVPE LTERVIPMYA ANDINHLNFF QRILYMFSPK TDKVAT
Validated NT Sequence
aagtagcaac tttatctgtt ttaggggaaa acatgtacaa tattctttgg aagaaattga gatggtttat atcatttgct gcgtacatag gtataacgcg ttctgtaagt tctggtacct gtatgtgaag atgaccaact tcttgtcctt tttttactgg tgcagatatt gcattttcgt gtgaaaaaaa ggttttgact gtatcatatg agcctttagg atatagaata atcacatcgt tattgaacac agcttctaca tattttgcat caccgttttt aactattatt tccttggctt tactaccttt gtgaaagatg gtttggctgc taaaatcatt aaaagcataa tctagcaact gctttatttc agatatgcgt tctttttcag ttttaagacc gctgattaat atgaagatac gtctgccttc atgtttagca gctactatag acccgtatcc tcctgcatct gtatatatgc taattatccc atctattcta ttatctggtg acaataagtt gatgttttct tggtatatat tattgtactt aaaatctttt ttggagaata gatgataata ttcaggaaaa tcttcgaaaa ttcttactgt aagagttatt aagtcgcgta ttgacatgaa ttgattttta tcttgagcac ctattacatt gctgaaatga gattttgtta ggttaagttt ttgtgctata cgattcattt catcaacaaa gttctcctgt gaacctgcta cgccttctgc aagagtaata caggcgtcat ttgcagatgt tgtaataata ccattaatta aatctcttac tgctactagc tgtcccgccc ttaggaaaat ggagttacct cctttctgcc aagcctcttt actagtctta aatttatcct ccatttttac aaatccagct tttatgtagt aaaatgtaac atataaggtc attaagttag tcatagacga gggtgcaatt tgttcgtcaa tgttgtgttc taataacatt gtattagaaa agaaatcgaa tactgctgct tgaggtgctg ttgtttgtat aggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALPIQTTA PQAAVFDFFS NTMLLEHNID EQIAPSSMTN LMTLYVTFYY IKAGFVKMED KFKTSKEAWQ KGGNSIFLRA GQLVAVRDLI NGIITTSAND ACITLAEGVA GSQENFVDEM NRIAQKLNLT KSHFSNVIGA QDKNQFMSIR DLITLTVRIF EDFPEYYHLF SKKDFKYNNI YQENINLLSP DNRIDGIISI YTDAGGYGSI VAAKHEGRRI FILISGLKTE KERISEIKQL LDYAFNDFSS QTIFHKGSKA KEIIVKNGDA KYVEAVFNND VIILYPKGSY DTVKTFFSHE NAISAPVKKG QEVGHLHIQV PELTERVIPM YAANDINHLN FFQRILYMFS PKTDKVATS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccttacct atacaaacaa cagcacctca agcagcagta ttcgatttct tttctaatac aatgttatta gaacacaaca ttgacgaaca aattgcaccc tcgtctatga ctaacttaat gaccttatat gttacatttt actacataaa agctggattt gtaaaaatgg aggataaatt taagactagt aaagaggctt ggcagaaagg aggtaactcc attttcctaa gggcgggaca gctagtagca gtaagagatt taattaatgg tattattaca acatctgcaa atgacgcctg tattactctt gcagaaggcg tagcaggttc acaggagaac tttgttgatg aaatgaatcg tatagcacaa aaacttaacc taacaaaatc tcatttcagc aatgtaatag gtgctcaaga taaaaatcaa ttcatgtcaa tacgcgactt aataactctt acagtaagaa ttttcgaaga ttttcctgaa tattatcatc tattctccaa aaaagatttt aagtacaata atatatacca agaaaacatc aacttattgt caccagataa tagaatagat gggataatta gcatatatac agatgcagga ggatacgggt ctatagtagc tgctaaacat gaaggcagac gtatcttcat attaatcagc ggtcttaaaa ctgaaaaaga acgcatatct gaaataaagc agttgctaga ttatgctttt aatgatttta gcagccaaac catctttcac aaaggtagta aagccaagga aataatagtt aaaaacggtg atgcaaaata tgtagaagct gtgttcaata acgatgtgat tattctatat cctaaaggct catatgatac agtcaaaacc tttttttcac acgaaaatgc aatatctgca ccagtaaaaa aaggacaaga agttggtcat cttcacatac aggtaccaga acttacagaa cgcgttatac ctatgtacgc agcaaatgat ataaaccatc tcaatttctt ccaaagaata ttgtacatgt tttcccctaa aacagataaa gttgctactt cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EhchA.00369.a.AE2.GE43222
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 41kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 100
Validated AA Sequence
MMALPIQTTA PQAAXFDFFS XTXXLEXXID EXIAPSSMTN LMTLYVTFYY XKAGFVXXED KFKTSKEAWQ KGGNSIFLRA GXLVAVRDLI NGIITTSAND ACITLAEGVA GSQENFVDEM NRIXQKLNLT KSHFSNVIGA QDKNQFMSIR DLITLTVRXX EDFPEYYHLF SKKDFKYNNI YQENINLLSP DNRIDGIISI YTDAGGYGSI VAAKHEGRRI FILISGLKTE KERISEIKQL LDYAFNDFSS QTIFHKGSKA KEIIVKNGDA KYVEAVFNNX VIILYPKGSY DTVKTXFSHE NAIXAPVKKG QEVGHLHIQV PELTERVIPM YAANDINHXN FFQRILYMFS PKTDKVATSA GHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctgaagtagc aactttatct gttttagggg aaaacatgta caatattctt tggaagaaat tganatggtt tatatcattt gctgcgtaca taggtataac gcgttctgta agttctggta cctgtatgtg aagatgacca acttcttgtc ctttttttac tggtgcanat attgcatttt cgtgtgaaaa anaggttttg actgtatcat atgagccttt aggatataga ataatcacnt cgttattgaa cacagcttct acatattttg catcaccgtt tttaactatt atttccttgg ctttactacc tttgtgaaag atggtttggc tgctaaaatc attaaaagca taatctagca actgctttat ttcagatatg cgttcttttt cagttttaag accgctgatt aatatgaaga tacgtctgcc ttcatgttta gcagctacta tagacccgta tcctcctgca tctgtatata tgctaattat cccatctatt ctattatctg gtgacaataa gttgatgttt tcttggtata tattattgta cttaaaatct tttttggaga atagatgata atattcagga aaatcttcgn nnattcttac tgtaagagtt attaagtcgc gtattgacat gaattgattt ttatcttgag cacctattac attgctgaaa tgagattttg ttaggttaag tttttgtnct atacgattca tttcatcaac aaagttctcc tgtgaacctg ctacgccttc tgcaagagta atacaggcgt cattngcaga tgttgtaata atnccattaa ttaaatctct tactgctact agctntcccg cccttaggaa aatggagtta cctcctttct gccaagcctc tttactagtc ttaaatttat cctccannnt tacaaatcca gcttttangt agtaaaatgt aacatataag gtcattaagt tagtcataga cgagggtgca attngttcgt caatgtngng ttctaannnc ntngtantag aaaagaaatc gaatnctgct gcttgaggtg ctgttgtttg tataggtaag gccatcatgg gagaga
Expected Protein Sequence
ALPIQTTAPQ AAVFDFFSNT MLLEHNIDEQ IAPSSMTNLM TLYVTFYYIK AGFVKMEDKF KTSKEAWQKG GNSIFLRAGQ LVAVRDLING IITTSANDAC ITLAEGVAGS QENFVDEMNR IAQKLNLTKS HFSNVIGAQD KNQFMSIRDL ITLTVRIFED FPEYYHLFSK KDFKYNNIYQ ENINLLSPDN RIDGIISIYT DAGGYGSIVA AKHEGRRIFI LISGLKTEKE RISEIKQLLD YAFNDFSSQT IFHKGSKAKE IIVKNGDAKY VEAVFNNDVI ILYPKGSYDT VKTFFSHENA ISAPVKKGQE VGHLHIQVPE LTERVIPMYA ANDINHLNFF QRILYMFSPK TDKVATSGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgc cttacctata caaacaacag cacctcaagc agcagtattc gatttctttt ctaatacaat gttattagaa cacaacattg acgaacaaat tgcaccctcg tctatgacta acttaatgac cttatatgtt acattttact acataaaagc tggatttgta aaaatggagg ataaatttaa gactagtaaa gaggcttggc agaaaggagg taactccatt ttcctaaggg cgggacagct agtagcagta agagatttaa ttaatggtat tattacaaca tctgcaaatg acgcctgtat tactcttgca gaaggcgtag caggttcaca ggagaacttt gttgatgaaa tgaatcgtat agcacaaaaa cttaacctaa caaaatctca tttcagcaat gtaataggtg ctcaagataa aaatcaattc atgtcaatac gcgacttaat aactcttaca gtaagaattt tcgaagattt tcctgaatat tatcatctat tctccaaaaa agattttaag tacaataata tataccaaga aaacatcaac ttattgtcac cagataatag aatagatggg ataattagca tatatacaga tgcaggagga tacgggtcta tagtagctgc taaacatgaa ggcagacgta tcttcatatt aatcagcggt cttaaaactg aaaaagaacg catatctgaa ataaagcagt tgctagatta tgcttttaat gattttagca gccaaaccat ctttcacaaa ggtagtaaag ccaaggaaat aatagttaaa aacggtgatg caaaatatgt agaagctgtg ttcaataacg atgtgattat tctatatcct aaaggctcat atgatacagt caaaaccttt ttttcacacg aaaatgcaat atctgcacca gtaaaaaaag gacaagaagt tggtcatctt cacatacagg taccagaact tacagaacgc gttataccta tgtacgcagc aaatgatata aaccatctca atttcttcca aagaatattg tacatgtttt cccctaaaac agataaagtt gctacttcag ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for EhchA.00369.a.A2.PW30723
PURIFICATION DATe: 3/4/2011
CONCENTRATION: 37.46mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Protocol Notes
notes unavailable
Validated AA Sequence
MLLEHNIDEQ IAPSSMTNLM TLYVTFYYIK AGFVKMEDKF KTSKEAWQKG GNSIFLRAGQ LVAVRDLING IITTSANDAC ITLAEGVAGS QENFVDEMNR IAQKLNLTKS HFSNVIGAQD KNQFMSIRDL ITLTVRIFED FPEYYHLFSK KDFKYNNIYQ ENINLLSPDN RIDGIISIYT DAGGYGSIVA AKHEGRRIFI LISGLKTEKE RISEIKQLLD YAFNDFSSQT IFHKGSKAKE IIVKNGDAKY VEAVFNNDVI ILYPKGSYDT VKTFFSHENA ISAPVKKGQE VGHLHIQVPE LTERVIPMYA ANDINHLNFF QRILYMFSPK TDKVAT
Validated NT Sequence
aagtagcaac tttatctgtt ttaggggaaa acatgtacaa tattctttgg aagaaattga gatggtttat atcatttgct gcgtacatag gtataacgcg ttctgtaagt tctggtacct gtatgtgaag atgaccaact tcttgtcctt tttttactgg tgcagatatt gcattttcgt gtgaaaaaaa ggttttgact gtatcatatg agcctttagg atatagaata atcacatcgt tattgaacac agcttctaca tattttgcat caccgttttt aactattatt tccttggctt tactaccttt gtgaaagatg gtttggctgc taaaatcatt aaaagcataa tctagcaact gctttatttc agatatgcgt tctttttcag ttttaagacc gctgattaat atgaagatac gtctgccttc atgtttagca gctactatag acccgtatcc tcctgcatct gtatatatgc taattatccc atctattcta ttatctggtg acaataagtt gatgttttct tggtatatat tattgtactt aaaatctttt ttggagaata gatgataata ttcaggaaaa tcttcgaaaa ttcttactgt aagagttatt aagtcgcgta ttgacatgaa ttgattttta tcttgagcac ctattacatt gctgaaatga gattttgtta ggttaagttt ttgtgctata cgattcattt catcaacaaa gttctcctgt gaacctgcta cgccttctgc aagagtaata caggcgtcat ttgcagatgt tgtaataata ccattaatta aatctcttac tgctactagc tgtcccgccc ttaggaaaat ggagttacct cctttctgcc aagcctcttt actagtctta aatttatcct ccatttttac aaatccagct tttatgtagt aaaatgtaac atataaggtc attaagttag tcatagacga gggtgcaatt tgttcgtcaa tgttgtgttc taataacatt gtattagaaa agaaatcgaa tactgctgct tgaggtgctg ttgtttgtat aggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALPIQTTA PQAAVFDFFS NTMLLEHNID EQIAPSSMTN LMTLYVTFYY IKAGFVKMED KFKTSKEAWQ KGGNSIFLRA GQLVAVRDLI NGIITTSAND ACITLAEGVA GSQENFVDEM NRIAQKLNLT KSHFSNVIGA QDKNQFMSIR DLITLTVRIF EDFPEYYHLF SKKDFKYNNI YQENINLLSP DNRIDGIISI YTDAGGYGSI VAAKHEGRRI FILISGLKTE KERISEIKQL LDYAFNDFSS QTIFHKGSKA KEIIVKNGDA KYVEAVFNND VIILYPKGSY DTVKTFFSHE NAISAPVKKG QEVGHLHIQV PELTERVIPM YAANDINHLN FFQRILYMFS PKTDKVATS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCTTACCT ATACAAACAA CAGCACCTCA AGCAGCAGTA TTCGATTTCT TTTCTAATAC AATGTTATTA GAACACAACA TTGACGAACA AATTGCACCC TCGTCTATGA CTAACTTAAT GACCTTATAT GTTACATTTT ACTACATAAA AGCTGGATTT GTAAAAATGG AGGATAAATT TAAGACTAGT AAAGAGGCTT GGCAGAAAGG AGGTAACTCC ATTTTCCTAA GGGCGGGACA GCTAGTAGCA GTAAGAGATT TAATTAATGG TATTATTACA ACATCTGCAA ATGACGCCTG TATTACTCTT GCAGAAGGCG TAGCAGGTTC ACAGGAGAAC TTTGTTGATG AAATGAATCG TATAGCACAA AAACTTAACC TAACAAAATC TCATTTCAGC AATGTAATAG GTGCTCAAGA TAAAAATCAA TTCATGTCAA TACGCGACTT AATAACTCTT ACAGTAAGAA TTTTCGAAGA TTTTCCTGAA TATTATCATC TATTCTCCAA AAAAGATTTT AAGTACAATA ATATATACCA AGAAAACATC AACTTATTGT CACCAGATAA TAGAATAGAT GGGATAATTA GCATATATAC AGATGCAGGA GGATACGGGT CTATAGTAGC TGCTAAACAT GAAGGCAGAC GTATCTTCAT ATTAATCAGC GGTCTTAAAA CTGAAAAAGA ACGCATATCT GAAATAAAGC AGTTGCTAGA TTATGCTTTT AATGATTTTA GCAGCCAAAC CATCTTTCAC AAAGGTAGTA AAGCCAAGGA AATAATAGTT AAAAACGGTG ATGCAAAATA TGTAGAAGCT GTGTTCAATA ACGATGTGAT TATTCTATAT CCTAAAGGCT CATATGATAC AGTCAAAACC TTTTTTTCAC ACGAAAATGC AATATCTGCA CCAGTAAAAA AAGGACAAGA AGTTGGTCAT CTTCACATAC AGGTACCAGA ACTTACAGAA CGCGTTATAC CTATGTACGC AGCAAATGAT ATAAACCATC TCAATTTCTT CCAAAGAATA TTGTACATGT TTTCCCCTAA AACAGATAAA GTTGCTACTT CATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA