SSGCID: Target Page

EhchA.00336.a

ornithine carbamoyltransferase

CENTER ID:	EhchA.00336.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00336.a.A1.GE26770	Full length( EhchA.00336.a )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00336.a.A1.PS00309	Full length( EhchA.00336.a )		1	303

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.68
RefSeq:	YP_506907.1
UniProt:	Q2GI26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKANICSFT DLNLHTKEEL RTILDYSSII KKKPNQYSKH LLNKNIALIF LQPSTRTRIS FEVGINQLGG NAIILNEKDT QLTRGESIYD TAKVLSRYVD LIVIRILEHN SLLELDKHST VPVINSLTQH SHPCQVLADI MTYEEKTGPI ENATVSWIGD FNNMLVSWIH AATVLNFKLN ISTLEYSKNL DLLLKSAQEK GNNITYDANP HNIISDADII TTDSWISMGY ENNIKTEFFK YQVNAKLLEK AKPNYMFLHC LPAHRGEEVT SEIIDGPNSY IFDEAENRLH IQKAIMLWCL NLL

NT Sequence

ATGAATAAAG CAAATATTTG TTCTTTTACA GATCTTAATT TACATACAAA AGAGGAACTA AGAACAATCT TAGACTATAG TTCTATAATC AAAAAAAAAC CTAACCAATA TAGCAAACAC CTATTGAATA AAAATATAGC TTTAATATTT CTGCAGCCAT CAACCCGTAC AAGGATATCA TTTGAAGTTG GAATAAACCA ACTAGGTGGA AATGCTATTA TTCTTAATGA AAAGGATACT CAATTGACAA GAGGAGAATC CATCTACGAT ACAGCTAAAG TTTTATCAAG ATATGTTGAT TTAATAGTAA TAAGGATATT AGAACATAAT TCTCTACTTG AATTAGACAA ACACAGCACA GTTCCAGTCA TTAATAGCCT CACACAACAT AGTCACCCAT GTCAAGTCTT AGCTGACATT ATGACTTACG AAGAAAAGAC AGGCCCTATA GAAAATGCTA CAGTAAGTTG GATTGGAGAT TTTAACAATA TGCTAGTGTC TTGGATACAT GCTGCAACAG TATTAAACTT CAAACTAAAC ATTTCAACTT TAGAATATTC AAAAAATCTT GATTTACTGC TTAAGTCAGC ACAAGAGAAA GGTAATAATA TCACATATGA TGCAAATCCT CATAACATCA TTTCAGACGC AGATATTATT ACTACAGACT CTTGGATTTC AATGGGTTAT GAAAATAATA TCAAAACCGA GTTTTTCAAA TACCAAGTTA ATGCCAAACT TCTTGAAAAA GCTAAACCTA ACTACATGTT TTTACATTGT TTACCAGCAC ACAGAGGAGA AGAAGTAACA TCTGAAATAA TAGATGGACC AAATTCATAT ATATTTGATG AAGCAGAAAA TAGATTACAT ATTCAAAAGG CAATCATGTT ATGGTGTTTA AATCTGCTA

Details for EhchA.00336.a.A1.GE26770

HARVESTED ON:	6/8/2009
SEQUENCED ON:	3/27/2012
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNKANICSF TDLNLHTKEE LRTILDYSSI IKKKPNQYSK HLLNKNIALI FLQPSTRTRI SFEVGINQLG GNAIILNEKD TQLTRGVSIY DTAKVLSRYV DLIVIRILEH NSLLELDKHS TVPVINSLTQ HSHPCQVLAD IMTYEEKTGP IENATVSWIG DFNNMLVSWI HAATVLNFKL NISTLEYSKN LDLLLKSAQE KGNNITYDAN PRNIISDADI ITTDSWISMG YENNIKTEFF KYQVNAKLLE KAKPNYMFLH CLPAHRGEEV TSEITDGPNS YIFDEAENRL HIQKAIMLWC LNLL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata aagcaaatat ttgttctttt acagatctta atttacatac aaaagaggaa ctaagaacaa tcttagacta cagttctata atcaaaaaaa aacctaacca atatagcaaa cacctattga ataaaaatat agctttaata tttctgcagc catcaacccg tacaaggata tcatttgaag ttggaataaa ccaactaggt ggaaatgcta ttattcttaa tgaaaaggat actcaattga caagaggagt atccatctac gatacagcta aagttttatc aagatatgtt gatttaatag taataaggat attagaacat aattctctac ttgaattaga caaacacagc acagttccag tcattaatag cctcacacaa catagtcacc catgtcaagt cttagctgac attatgactt acgaagaaaa gacaggccct atagaaaatg ctacagtaag ttggattgga gattttaaca atatgctagt gtcttggata catgctgcaa cagtattaaa cttcaaacta aacatttcaa ctttagaata ttcaaaaaat cttgatttgc tgcttaagtc agcacaagag aaaggtaata atatcacata tgatgcaaat cctcgtaaca tcatttcaga cgcagatatt attactacag actcttggat ttcaatgggt tatgaaaata atatcaaaac cgagtttttc aaataccaag ttaatgccaa acttcttgaa aaagctaaac ctaactacat gtttttacat tgtttaccag cacacagagg agaagaagta acatctgaaa taacagatgg accaaattca tatatatttg atgaagcaga aaatagatta catattcaaa aggcaatcat gttatggtgt ttaaatctgc tataataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNKANICSF TDLNLHTKEE LRTILDYSSI IKKKPNQYSK HLLNKNIALI FLQPSTRTRI SFEVGINQLG GNAIILNEKD TQLTRGESIY DTAKVLSRYV DLIVIRILEH NSLLELDKHS TVPVINSLTQ HSHPCQVLAD IMTYEEKTGP IENATVSWIG DFNNMLVSWI HAATVLNFKL NISTLEYSKN LDLLLKSAQE KGNNITYDAN PHNIISDADI ITTDSWISMG YENNIKTEFF KYQVNAKLLE KAKPNYMFLH CLPAHRGEEV TSEIIDGPNS YIFDEAENRL HIQKAIMLWC LNLL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaataaagca aatatttgtt cttttacaga tcttaattta catacaaaag aggaactaag aacaatctta gactatagtt ctataatcaa aaaaaaacct aaccaatata gcaaacacct attgaataaa aatatagctt taatatttct gcagccatca acccgtacaa ggatatcatt tgaagttgga ataaaccaac taggtggaaa tgctattatt cttaatgaaa aggatactca attgacaaga ggagaatcca tctacgatac agctaaagtt ttatcaagat atgttgattt aatagtaata aggatattag aacataattc tctacttgaa ttagacaaac acagcacagt tccagtcatt aatagcctca cacaacatag tcacccatgt caagtcttag ctgacattat gacttacgaa gaaaagacag gccctataga aaatgctaca gtaagttgga ttggagattt taacaatatg ctagtgtctt ggatacatgc tgcaacagta ttaaacttca aactaaacat ttcaacttta gaatattcaa aaaatcttga tttactgctt aagtcagcac aagagaaagg taataatatc acatatgatg caaatcctca taacatcatt tcagacgcag atattattac tacagactct tggatttcaa tgggttatga aaataatatc aaaaccgagt ttttcaaata ccaagttaat gccaaacttc ttgaaaaagc taaacctaac tacatgtttt tacattgttt accagcacac agaggagaag aagtaacatc tgaaataata gatggaccaa attcatatat atttgatgaa gcagaaaata gattacatat tcaaaaggca atcatgttat ggtgtttaaa tctgctaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for EhchA.00336.a.A1.PS00309

PURIFICATION DATe:	7/31/2009
CONCENTRATION:	27.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	50µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATAAAGCA AATATTTGTT CTTTTACAGA TCTTAATTTA CATACAAAAG AGGAACTAAG AACAATCTTA GACTATAGTT CTATAATCAA AAAAAAACCT AACCAATATA GCAAACACCT ATTGAATAAA AATATAGCTT TAATATTTCT GCAGCCATCA ACCCGTACAA GGATATCATT TGAAGTTGGA ATAAACCAAC TAGGTGGAAA TGCTATTATT CTTAATGAAA AGGATACTCA ATTGACAAGA GGAGAATCCA TCTACGATAC AGCTAAAGTT TTATCAAGAT ATGTTGATTT AATAGTAATA AGGATATTAG AACATAATTC TCTACTTGAA TTAGACAAAC ACAGCACAGT TCCAGTCATT AATAGCCTCA CACAACATAG TCACCCATGT CAAGTCTTAG CTGACATTAT GACTTACGAA GAAAAGACAG GCCCTATAGA AAATGCTACA GTAAGTTGGA TTGGAGATTT TAACAATATG CTAGTGTCTT GGATACATGC TGCAACAGTA TTAAACTTCA AACTAAACAT TTCAACTTTA GAATATTCAA AAAATCTTGA TTTACTGCTT AAGTCAGCAC AAGAGAAAGG TAATAATATC ACATATGATG CAAATCCTCA TAACATCATT TCAGACGCAG ATATTATTAC TACAGACTCT TGGATTTCAA TGGGTTATGA AAATAATATC AAAACCGAGT TTTTCAAATA CCAAGTTAAT GCCAAACTTC TTGAAAAAGC TAAACCTAAC TACATGTTTT TACATTGTTT ACCAGCACAC AGAGGAGAAG AAGTAACATC TGAAATAATA GATGGACCAA ATTCATATAT ATTTGATGAA GCAGAAAATA GATTACATAT TCAAAAGGCA ATCATGTTAT GGTGTTTAAA TCTGCTAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA