EhchA.00256.a

30S ribosomal protein S4

CENTER ID:	EhchA.00256.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00256.a.A1.GE26746	Full length( EhchA.00256.a )		1	202

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131566
PATRIC ID:	fig\|205920.11.peg.789
RefSeq:	YP_507703.1
UniProt:	Q2GFT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVIQRKYRAS RRLGVNLWGR SKDPFNTRNY PPGQHGGMGY KKPSDFGKQF AAHKKFKFYY AINSKQMRNI FLKAYKKRGD TGDNFVGLLE SRLSSVLYNS GLVPTIFSAR QLISHKHVLV NGKTVNISSY VVKVGDVVTL KEKAKNLPAV VFAVQSQEQK VPDYLEVDTQ EKSIRYLRVP KYCEVPYPAT MEVNLVIEFY SR

NT Sequence

ATGGTAATAC AGAGAAAATA TAGAGCAAGT CGTAGGTTAG GTGTAAATCT TTGGGGTAGA TCTAAGGATC CATTTAATAC AAGGAATTAT CCTCCTGGTC AGCATGGCGG TATGGGTTAT AAGAAACCTT CTGACTTTGG TAAGCAGTTT GCAGCGCATA AGAAATTTAA GTTTTATTAT GCTATAAATA GTAAGCAAAT GCGTAATATT TTCTTGAAAG CTTATAAGAA AAGAGGCGAT ACTGGTGATA ATTTTGTTGG ATTATTGGAA TCTAGGTTAT CGAGTGTTTT GTATAATTCT GGGCTTGTAC CAACTATTTT TTCTGCAAGG CAGCTAATAT CCCATAAACA TGTTTTGGTT AACGGTAAAA CAGTAAATAT ATCTAGCTAT GTTGTGAAAG TAGGTGATGT AGTTACACTA AAAGAAAAAG CAAAAAATTT ACCTGCAGTT GTTTTTGCAG TGCAATCTCA AGAGCAAAAG GTACCTGACT ACTTAGAAGT CGATACTCAA GAAAAATCTA TAAGATATTT GAGGGTACCT AAGTATTGTG AAGTACCGTA TCCAGCAACT ATGGAAGTTA ATTTGGTAAT AGAGTTCTAC TCTAGG

Details for EhchA.00256.a.A1.GE26746

HARVESTED ON:	6/8/2009
SEQUENCED ON:	3/27/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVIQRKYRA SRRLGVNLWG RSKDPFNTRN YPPGQHGGMG YKKPSDFGKQ FAAHKKFKFY YAINSKQMRN IFLKAYKKRG DTGDNFVGLL ESRLSSVLYN SGLVPTIFSA RQLISHKHVL VNGKTVNISS YVVKVGDVVT LKEKAKNLPA VVFAVQSQEQ KVPDYLEVDT QEKSIRYLRV PKYCEVPYPA TMEVNLVIEF YSR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtaa tacagagaaa atatagagca agtcgtaggt taggtgtaaa tctttggggt agatctaagg atccatttaa tacaaggaat tatcctcctg gtcagcatgg cggtatgggt tataagaaac cttctgactt tggtaagcag tttgcagcgc ataagaaatt taagttttat tatgctataa atagtaagca aatgcgtaat attttcttga aagcttataa gaaaagaggc gatactggtg ataattttgt tggattattg gaatctaggt tatcgagtgt tttgtataat tctgggcttg taccaactat tttttctgca aggcagctaa tatcccataa acatgttttg gttaacggta aaacagtaaa tatatctagc tatgttgtga aagtaggtga tgtagttaca ctaaaagaaa aagcaaaaaa tttacctgca gttgtttttg cagtgcaatc tcaagagcaa aaggtacctg actacttaga agtcgatact caagaaaaat ctataagata tttgagggta cctaagtatt gtgaagtacc gtatccagca actatggaag ttaatttggt aatagagttc tactctaggt aataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaatacag agaaaatata gagcaagtcg taggttaggt gtaaatcttt ggggtagatc taaggatcca tttaatacaa ggaattatcc tcctggtcag catggcggta tgggttataa gaaaccttct gactttggta agcagtttgc agcgcataag aaatttaagt tttattatgc tataaatagt aagcaaatgc gtaatatttt cttgaaagct tataagaaaa gaggcgatac tggtgataat tttgttggat tattggaatc taggttatcg agtgttttgt ataattctgg gcttgtacca actatttttt ctgcaaggca gctaatatcc cataaacatg ttttggttaa cggtaaaaca gtaaatatat ctagctatgt tgtgaaagta ggtgatgtag ttacactaaa agaaaaagca aaaaatttac ctgcagttgt ttttgcagtg caatctcaag agcaaaaggt acctgactac ttagaagtcg atactcaaga aaaatctata agatatttga gggtacctaa gtattgtgaa gtaccgtatc cagcaactat ggaagttaat ttggtaatag agttctactc taggaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa