EhchA.00219.a

3,4-dihydroxy-2-butanone 4-phosphate synthase

CENTER ID:	EhchA.00219.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.00219.a.A1.GE26764	Full length( EhchA.00219.a )		1	211

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127275
PATRIC ID:	fig\|205920.11.peg.112
RefSeq:	YP_506962.1
UniProt:	Q2GHX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGIIVSENSS FISPVEHIIE DSKQGKMFIL IDDEQRENEG DLVVLAEKVT SGVINFMITH GSGIVCLSIT EDLMHKLGLE FIPKRHVNCN SASFATSIDA RYGITTGVSA HDRAHTILTV VNKNVTEDDF VTPGHIFPIV SKKGGVLERS GHTEASVEIA RIAGLAPVAV VCELMNPDGT MSRMPDIIEF SQRYNIKVTS IDKLIQYVKA N

NT Sequence

ATGGGTATTA TTGTATCAGA AAATTCGAGT TTTATCTCTC CTGTAGAGCA TATTATTGAA GATTCTAAAC AAGGTAAAAT GTTTATTTTG ATTGATGATG AACAAAGGGA AAATGAAGGT GATTTGGTAG TTTTAGCAGA AAAGGTAACA AGTGGTGTAA TTAACTTTAT GATAACTCAT GGTAGTGGTA TTGTGTGTTT ATCTATTACA GAAGATTTAA TGCATAAATT GGGGTTGGAG TTTATACCAA AGAGGCATGT TAATTGTAAT AGTGCGTCTT TTGCAACTTC TATAGATGCT CGTTATGGTA TTACAACTGG TGTTTCTGCT CATGATAGAG CCCATACTAT ACTTACAGTT GTTAATAAAA ATGTTACAGA GGATGATTTT GTAACACCGG GGCATATTTT TCCTATTGTA TCTAAAAAAG GTGGAGTGTT AGAGAGATCT GGTCATACAG AAGCGAGTGT TGAAATTGCA AGAATTGCAG GGTTAGCTCC AGTAGCTGTA GTATGTGAGT TGATGAATCC TGATGGAACA ATGTCAAGGA TGCCTGATAT TATAGAGTTT TCCCAGCGTT ATAACATTAA AGTTACTAGT ATAGATAAAC TTATTCAATA TGTAAAAGCA AAC

Details for EhchA.00219.a.A1.GE26764

HARVESTED ON:	6/18/2009
SEQUENCED ON:	3/27/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGIIVSENS SFISPVEHII EDSKQGKMFI LIDDEQRENE GDLVVLAEKV TSGVINFMIT HGSGIVCLSI TEDLMHKLGL EFIPKRHVNC NSASFATSID ARYGITAGVS AHDRAHTILT VVNKNVTEDD FVTPGHIFPI VSKKGGVLER SGHTEASVEI ARIAGLAPVA VVCELMNPDG TMSRMPDIIE FSQRYNIKVT SIDKLIQYVK AN

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggta ttattgtatc agaaaattcg agttttatct ctcctgtaga gcatattatt gaagattcta aacaaggtaa aatgtttatt ttgattgatg atgaacaaag ggaaaatgaa ggtgatttgg tagttttagc agaaaaggta acaagtggtg taattaactt tatgataact catggtagtg gtattgtgtg tttatctatt acagaagatt taatgcataa attggggttg gagtttatac caaagaggca tgttaattgt aatagtgcgt cttttgcaac ttctatagat gctcgttatg gtattacagc tggtgtttct gctcatgata gagcccatac tatacttaca gttgttaata aaaatgttac agaggatgat tttgtaacgc cggggcatat ttttcctatt gtatctaaaa aaggtggagt gttagagaga tctggtcata cagaagcgag tgttgaaatt gcaagaattg cagggttagc tccagtagct gtagtatgtg agttgatgaa tcctgatgga acaatgtcaa ggatgcctga tattatagag ttttcccagc gttataacat taaagttact agtatagata aacttattca atatgtaaaa gcaaactaat aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGIIVSENS SFISPVEHII EDSKQGKMFI LIDDEQRENE GDLVVLAEKV TSGVINFMIT HGSGIVCLSI TEDLMHKLGL EFIPKRHVNC NSASFATSID ARYGITTGVS AHDRAHTILT VVNKNVTEDD FVTPGHIFPI VSKKGGVLER SGHTEASVEI ARIAGLAPVA VVCELMNPDG TMSRMPDIIE FSQRYNIKVT SIDKLIQYVK AN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtattatt gtatcagaaa attcgagttt tatctctcct gtagagcata ttattgaaga ttctaaacaa ggtaaaatgt ttattttgat tgatgatgaa caaagggaaa atgaaggtga tttggtagtt ttagcagaaa aggtaacaag tggtgtaatt aactttatga taactcatgg tagtggtatt gtgtgtttat ctattacaga agatttaatg cataaattgg ggttggagtt tataccaaag aggcatgtta attgtaatag tgcgtctttt gcaacttcta tagatgctcg ttatggtatt acaactggtg tttctgctca tgatagagcc catactatac ttacagttgt taataaaaat gttacagagg atgattttgt aacaccgggg catatttttc ctattgtatc taaaaaaggt ggagtgttag agagatctgg tcatacagaa gcgagtgttg aaattgcaag aattgcaggg ttagctccag tagctgtagt atgtgagttg atgaatcctg atggaacaat gtcaaggatg cctgatatta tagagttttc ccagcgttat aacattaaag ttactagtat agataaactt attcaatatg taaaagcaaa caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa