EhchA.00211.a

putative pyrroline-5-carboxylate reductase

CENTER ID: EhchA.00211.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00211.a.A1.GE26715 Full length( EhchA.00211.a ) 1 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.00211.a.A1.PS00338a Full length( EhchA.00211.a ) 1 269

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.13
RefSeq: YP_506848.1
UniProt: Q2GI85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNILLIGCGN LGSILLNKWS HSDQISKVTV VQPSLSKKDT FNNITKVVFV KSHEEVDKDF IPQIVVIAIK PQLVSDVMPN YRIYSESASF ISLCAGIDIN FLKTCLSPTA TIIRVMPNIA MSLGKSVNLS YIEEELPHEK QKVSTIFNNT GLIYWIQKED IFDKLSPITG SGPAYFFSLA EELVKNTIAA GINEEDAIKL VSQTFIGSAK LLETRQNTIS HLISSITSRG GITQAALQVL NLHLPQTISS CLKAAMERLL YLKGLDKNK
NT Sequence
ATGAACATAT TACTAATAGG ATGCGGTAAC TTAGGAAGTA TATTATTAAA TAAATGGTCA CATAGTGATC AAATATCAAA AGTGACTGTA GTACAGCCTA GCTTATCAAA AAAGGATACT TTTAACAATA TAACTAAGGT AGTCTTTGTA AAAAGTCATG AGGAAGTAGA TAAAGATTTT ATACCTCAAA TTGTAGTAAT TGCAATAAAA CCACAACTGG TGTCTGATGT TATGCCAAAC TACAGAATTT ATAGTGAATC TGCATCTTTT ATATCTTTAT GTGCCGGAAT TGATATTAAT TTTCTAAAAA CATGCTTAAG TCCTACAGCA ACAATAATTA GGGTAATGCC TAATATTGCC ATGTCACTAG GCAAAAGTGT AAACCTTTCT TATATAGAAG AAGAATTACC ACATGAAAAA CAAAAAGTAT CAACTATTTT TAATAACACA GGACTTATAT ATTGGATACA AAAAGAAGAT ATCTTTGACA AATTATCTCC TATTACTGGT TCTGGTCCTG CATACTTTTT TTCTCTTGCA GAAGAATTAG TAAAAAATAC TATAGCTGCA GGTATCAATG AAGAAGATGC TATTAAACTT GTATCTCAAA CATTTATAGG AAGTGCTAAA TTGCTAGAAA CAAGACAAAA TACTATAAGT CATCTTATAT CATCTATAAC AAGCCGCGGC GGAATAACAC AAGCTGCACT GCAGGTGTTA AATTTACATT TACCTCAAAC AATTAGTTCA TGTTTAAAGG CAGCCATGGA AAGGCTATTA TACTTAAAAG GATTAGATAA AAATAAA
Details for EhchA.00211.a.A1.GE26715
HARVESTED ON: 6/1/2009
SEQUENCED ON: 3/27/2012
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNILLIGCG NLGSILLNKW SHSDQISKVT VVQPSLSKKD TFNNITKVVF VKSHEEVDKD FIPQIVVIAI KPQLVSDVMP NYRIYSESAS FISLCAGIDI NFLKTCLSPT ATIIRVMPNI AMSLGKSVNL SYIEEELPHE KQKVSTIFNN TGLIYWIQKE DIFDKLSPIT GSGPAYFFSL AEELVKNTIA AGINEEDAIK LVSQTFIGSA KLLETRQNTI SHLISSITSR GGITQAALQV LNLHLPQTIS SCLKAAMERL LYLKGLDK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca tattactaat aggatgcggt aacttaggaa gtatattatt aaataaatgg tcacatagtg atcaaatatc aaaagtgact gtagtacagc ctagcttatc aaaaaaggat acttttaaca atataactaa ggtagtcttt gtaaaaagtc atgaggaagt agataaagat tttatacctc aaattgtagt aattgcaata aaaccacaac tggtgtctga tgttatgcca aactacagaa tttatagtga atctgcatct tttatatctt tatgtgccgg aattgatatt aattttctaa aaacatgctt aagtcctaca gcaacaataa ttagggtaat gcctaatatt gccatgtcac taggcaaaag tgtaaacctt tcttatatag aagaagaatt accacatgaa aaacaaaaag tatcaactat ttttaataac acaggactta tatattggat acaaaaagaa gatatctttg acaaattatc tcctattact ggttctggtc ctgcatactt tttttctctt gcagaagaat tagtaaaaaa tactatagct gcaggtatca atgaagaaga tgctattaaa cttgtatctc aaacatttat aggaagtgct aaattgctag aaacaagaca aaatactata agtcatctta tatcatctat aacaagccgc ggcggaataa cacaagctgc actgcaggtg ttaaatttac atttacctca aacaattagt tcatgtttaa aggcagccat ggaaaggcta ttatacttaa aaggattaga taaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNILLIGCG NLGSILLNKW SHSDQISKVT VVQPSLSKKD TFNNITKVVF VKSHEEVDKD FIPQIVVIAI KPQLVSDVMP NYRIYSESAS FISLCAGIDI NFLKTCLSPT ATIIRVMPNI AMSLGKSVNL SYIEEELPHE KQKVSTIFNN TGLIYWIQKE DIFDKLSPIT GSGPAYFFSL AEELVKNTIA AGINEEDAIK LVSQTFIGSA KLLETRQNTI SHLISSITSR GGITQAALQV LNLHLPQTIS SCLKAAMERL LYLKGLDKNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacatatta ctaataggat gcggtaactt aggaagtata ttattaaata aatggtcaca tagtgatcaa atatcaaaag tgactgtagt acagcctagc ttatcaaaaa aggatacttt taacaatata actaaggtag tctttgtaaa aagtcatgag gaagtagata aagattttat acctcaaatt gtagtaattg caataaaacc acaactggtg tctgatgtta tgccaaacta cagaatttat agtgaatctg catcttttat atctttatgt gccggaattg atattaattt tctaaaaaca tgcttaagtc ctacagcaac aataattagg gtaatgccta atattgccat gtcactaggc aaaagtgtaa acctttctta tatagaagaa gaattaccac atgaaaaaca aaaagtatca actattttta ataacacagg acttatatat tggatacaaa aagaagatat ctttgacaaa ttatctccta ttactggttc tggtcctgca tacttttttt ctcttgcaga agaattagta aaaaatacta tagctgcagg tatcaatgaa gaagatgcta ttaaacttgt atctcaaaca tttataggaa gtgctaaatt gctagaaaca agacaaaata ctataagtca tcttatatca tctataacaa gccgcggcgg aataacacaa gctgcactgc aggtgttaaa tttacattta cctcaaacaa ttagttcatg tttaaaggca gccatggaaa ggctattata cttaaaagga ttagataaaa ataaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EhchA.00211.a.A1.PS00338a
PURIFICATION DATe: 7/29/2009
CONCENTRATION: 55.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNILLIGCG NLGSILLNKW SHSDQISKVT VVQPSLSKKD TFNNITKVVF VKSHEEVDKD FIPQIVVIAI KPQLVSDVMP NYRIYSESAS FISLCAGIDI NFLKTCLSPT ATIIRVMPNI AMSLGKSVNL SYIEEELPHE KQKVSTIFNN TGLIYWIQKE DIFDKLSPIT GSGPAYFFSL AEELVKNTIA AGINEEDAIK LVSQTFIGSA KLLETRQNTI SHLISSITSR GGITQAALQV LNLHLPQTIS SCLKAAMERL LYLKGLDK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca tattactaat aggatgcggt aacttaggaa gtatattatt aaataaatgg tcacatagtg atcaaatatc aaaagtgact gtagtacagc ctagcttatc aaaaaaggat acttttaaca atataactaa ggtagtcttt gtaaaaagtc atgaggaagt agataaagat tttatacctc aaattgtagt aattgcaata aaaccacaac tggtgtctga tgttatgcca aactacagaa tttatagtga atctgcatct tttatatctt tatgtgccgg aattgatatt aattttctaa aaacatgctt aagtcctaca gcaacaataa ttagggtaat gcctaatatt gccatgtcac taggcaaaag tgtaaacctt tcttatatag aagaagaatt accacatgaa aaacaaaaag tatcaactat ttttaataac acaggactta tatattggat acaaaaagaa gatatctttg acaaattatc tcctattact ggttctggtc ctgcatactt tttttctctt gcagaagaat tagtaaaaaa tactatagct gcaggtatca atgaagaaga tgctattaaa cttgtatctc aaacatttat aggaagtgct aaattgctag aaacaagaca aaatactata agtcatctta tatcatctat aacaagccgc ggcggaataa cacaagctgc actgcaggtg ttaaatttac atttacctca aacaattagt tcatgtttaa aggcagccat ggaaaggcta ttatacttaa aaggattaga taaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNILLIGCG NLGSILLNKW SHSDQISKVT VVQPSLSKKD TFNNITKVVF VKSHEEVDKD FIPQIVVIAI KPQLVSDVMP NYRIYSESAS FISLCAGIDI NFLKTCLSPT ATIIRVMPNI AMSLGKSVNL SYIEEELPHE KQKVSTIFNN TGLIYWIQKE DIFDKLSPIT GSGPAYFFSL AEELVKNTIA AGINEEDAIK LVSQTFIGSA KLLETRQNTI SHLISSITSR GGITQAALQV LNLHLPQTIS SCLKAAMERL LYLKGLDKNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACATATTA CTAATAGGAT GCGGTAACTT AGGAAGTATA TTATTAAATA AATGGTCACA TAGTGATCAA ATATCAAAAG TGACTGTAGT ACAGCCTAGC TTATCAAAAA AGGATACTTT TAACAATATA ACTAAGGTAG TCTTTGTAAA AAGTCATGAG GAAGTAGATA AAGATTTTAT ACCTCAAATT GTAGTAATTG CAATAAAACC ACAACTGGTG TCTGATGTTA TGCCAAACTA CAGAATTTAT AGTGAATCTG CATCTTTTAT ATCTTTATGT GCCGGAATTG ATATTAATTT TCTAAAAACA TGCTTAAGTC CTACAGCAAC AATAATTAGG GTAATGCCTA ATATTGCCAT GTCACTAGGC AAAAGTGTAA ACCTTTCTTA TATAGAAGAA GAATTACCAC ATGAAAAACA AAAAGTATCA ACTATTTTTA ATAACACAGG ACTTATATAT TGGATACAAA AAGAAGATAT CTTTGACAAA TTATCTCCTA TTACTGGTTC TGGTCCTGCA TACTTTTTTT CTCTTGCAGA AGAATTAGTA AAAAATACTA TAGCTGCAGG TATCAATGAA GAAGATGCTA TTAAACTTGT ATCTCAAACA TTTATAGGAA GTGCTAAATT GCTAGAAACA AGACAAAATA CTATAAGTCA TCTTATATCA TCTATAACAA GCCGCGGCGG AATAACACAA GCTGCACTGC AGGTGTTAAA TTTACATTTA CCTCAAACAA TTAGTTCATG TTTAAAGGCA GCCATGGAAA GGCTATTATA CTTAAAAGGA TTAGATAAAA ATAAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA