EbzaA.19907.a
Envelope glycoprotein (GP)
| CENTER ID: | EbzaA.19907.a |
| ORGANISM: | Ebolavirus Zaire ebolavirus Mayinga-76 |
| ASSOCIATED DISEASE: | Hemorrhagic fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 4 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIA |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EbzaA.19907.a.HE11.PD38325 | full length | 32 | 632 | ||
| EbzaA.19907.a.HE11.PD38327 | full length | 32 | 632 | ||
| EbzaA.19907.a.HE11.PD38471 | full length | 32 | 632 |
Structures
6NAE
DEPOSITED: 12/5/2018
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38326
7LYD
DEPOSITED: 3/6/2021
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38351
7LYY
DEPOSITED: 3/8/2021
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38351
7M2D
DEPOSITED: 3/16/2021
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38351
7SSQ
DEPOSITED: 11/11/2021
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38458
7SSR
DEPOSITED: 11/11/2021
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38458
8F87
DEPOSITED: 11/21/2022
DETERMINATION: XRay
CLONE: EbzaA.19907.a.HE11.GE42284
PROTEIN: EbzaA.19907.a.HE11.PD38351
Publications by SSGCID
Discovery of Adamantane Carboxamides as Ebola Virus Cell Entry and Glycoprotein Inhibitors
Abendroth JA, Bullen J, Dranow DM, McCormack K, Smutney H
ACS Med Chem Lett - 2020
volume 11, issue 6, pages 1160-1167
PMID: 32550996; PMCID: PMC7294737
PDB:
6NAE
External Resources
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
[sequence withheld]
NT Sequence
[sequence withheld]
Details for EbzaA.19907.a.HE11.PD38325
| PURIFICATION DATe: | 7/25/2018 |
| CONCENTRATION: | 3.88mg/ml |
| OBSERVED MW: | 57kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM MES pH 5.2, 150mM NaCl |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 15µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGILPSPGMP ALLSLVSLLS VLLMGCVAET GRSIPLGVIH NSALQVSDVD KLVCRDKLSS TNQLRSVGLN LEGNGVATDV PSATKRWGFR SGVPPKVVNY EAGEWAENCY NLEIKKPDGS ECLPAAPDGI RGFPRCRYVH KVSGTGPCAG DFAFHKEGAF FLYDRLASTV IYRGTTFAEG VVAFLILPQA KKDFFSSHPL REPVNATEDP SSGYYSTTIR YQATGFGTNE TEYLFEVDNL TYVQLESRFT PQFLLQLNET IYTSGKRSNT TGKLIWKVNP EIDTTIGEWA FWETKKNLTR KIRSEELSFT VVSTHHQDTG EESASSGKLG LITNTIAGVA GLITGGRRTR REAIVNAQPK CNPNLHYWTT QDEGAAIGLA WIPYFGPAAE GIYIEGLMHN QDGLICGLRQ LANETTQALQ LFLRATTELR TFSILNRKAI DFLLQRWGGT CHILGPDCCI EPADWTKNIT DKIDQIIHDF VDGSGYIPEA PRDGQAYVRK DGEWVLLSTF LGTHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC GGAAACCCGT CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG AGTCCGCATC GACCGGATCG GAAAACCTCT CGAGAAAGGC GTCTAACCAG TCACAGTCGC AAGGTAGGCT GAGCACCGTG GCGGGCGGCA GCGGGTGGCG GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA TTAAAGTAGG CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGCAG GCTTGAGATC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT ACTTCTGCGC TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GATCTGGCCA TACACTTGAG TGACAATGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA AACGGATCTC TAGCGAATTC GCCGCCACCA TGGGAATCCT GCCCTCCCCC GGAATGCCTG CCCTGCTGTC CCTCGTGTCC CTTCTGTCCG TGCTGCTGAT GGGTTGCGTG GCGGAAACTG GTAGAAGCAT CCCTCTCGGC GTGATCCACA ACAGCGCCCT CCAAGTGTCA GACGTGGACA AGCTCGTCTG CCGCGATAAG CTCAGCTCCA CCAACCAGCT GCGGTCGGTC GGTCTGAACC TGGAAGGGAA CGGGGTGGCC ACCGACGTGC CGTCGGCGAC CAAGCGCTGG GGCTTTAGGT CGGGAGTGCC GCCCAAGGTC GTGAACTACG AGGCCGGCGA ATGGGCCGAA AACTGCTACA ATCTGGAAAT CAAGAAGCCT GACGGGTCCG AGTGCTTGCC GGCGGCCCCC GACGGCATCC GCGGATTCCC GCGGTGTAGA TACGTGCACA AAGTGTCCGG CACCGGCCCT TGCGCCGGGG ATTTCGCCTT TCATAAGGAA GGCGCTTTCT TCCTGTACGA CCGACTGGCC TCTACCGTGA TCTATCGGGG AACTACTTTC GCCGAGGGCG TGGTCGCATT CCTCATCCTG CCACAAGCTA AAAAGGACTT CTTTTCCTCC CACCCGCTGC GCGAACCCGT GAACGCCACC GAGGACCCAT CGTCCGGATA CTACTCGACC ACCATCCGGT ACCAGGCCAC CGGTTTCGGG ACTAACGAAA CCGAGTACCT CTTCGAAGTG GATAACCTGA CCTACGTGCA GCTGGAGTCC AGATTCACCC CGCAATTCCT CCTGCAACTC AACGAAACCA TCTACACCTC CGGAAAGAGA AGCAACACGA CTGGGAAGCT GATTTGGAAA GTCAATCCCG AGATTGACAC CACTATCGGA GAGTGGGCAT TCTGGGAAAC CAAGAAGAAC TTGACCAGAA AGATCCGGAG CGAAGAACTG TCGTTCACTG TGGTGTCCAC CCACCACCAG GACACCGGAG AGGAATCAGC CTCATCCGGA AAACTTGGAC TGATTACTAA CACGATTGCT GGTGTCGCTG GTCTTATCAC TGGCGGACGC CGGACCCGGC GGGAAGCGAT AGTGAACGCC CAGCCTAAGT GCAACCCAAA CCTCCACTAC TGGACGACAC AGGACGAGGG TGCCGCCATC GGGCTGGCCT GGATTCCTTA CTTCGGACCG GCGGCAGAGG GAATCTATAT TGAGGGCCTG ATGCATAACC AGGACGGCCT GATCTGTGGG CTGCGGCAAT TGGCAAATGA GACTACCCAG GCGCTGCAGC TGTTCTTGCG GGCCACTACC GAGCTTAGGA CTTTCAGCAT CCTGAACCGC AAGGCCATTG ATTTCCTGCT GCAACGCTGG GGCGGAACTT GCCACATCCT CGGACCCGAC TGCTGTATCG AGCCGGCCGA TTGGACAAAG AACATCACCG ACAAGATTGA TCAGATTATT CACGACTTCG TGGACGGCAG CGGATACATC CCGGAAGCCC CCAGGGATGG CCAGGCCTAC GTGCGCAAGG ATGGCGAATG GGTCCTGCTC TCCACCTTCC TCGGCACCCA CCATCATCAC CACCACTGAT AAGGTTGAGC GGCCGCTCGA GGCCGGCAAG GCCAGATCCC CCGACCTCGA CCTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTGGT CGAGATCCCT CGGAGATCTC TAGCTAGAGG ATCGATCCCC GCCCCGGACG AACTAAACCT GACTACGACA TCTCTGCCCC TTCTTCGCGG GGCAGTGCAT GTAATCCCTT CAGTTGGTTG GTACAACTTG CCAACTGAAC CCTAAACGGG TAGCATATGC TTCCCGGGTA GTAGTATATA CTATCCAGAC TAACCCTAAT TCAATAGCAT ATGTTACCCA ACGGGAAGCA TATGCTATCG AATTAGGGTT AGTAAAAGGG TCCTAAGGAA CAGCGATGTA GGTGGGCGGG CCAAGATAGG GGCGCGATTG CTGCGATCTG GAGGACAAAT TACACACACT TGCGCCTGAG CGCCAAGCAC AGGGTTGTTG GTCCTCATAT TCACGAGGTC GCTGAGAGCA CGGTGGGCTA ATGTTGCCAT GGGTAGCATA TACTACCCAA ATATCTGGAT AGCATATGCT ATCCTAATCT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATCT GTATCCGGGT AGCATATGCT ATCCTAATAG AGATTAGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATATACT ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA TATGCTATCC TCATGATAAG CTGTCAAACA TGAGAATTAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCA
Details for EbzaA.19907.a.HE11.PD38327
| PURIFICATION DATe: | 7/25/2018 |
| CONCENTRATION: | 4.79mg/ml |
| OBSERVED MW: | 57kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM MES pH 5.2, 150mM NaCl |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 15µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGILPSPGMP ALLSLVSLLS VLLMGCVAET GRSIPLGVIH NSALQVSDVD KLVCRDKLSS TNQLRSVGLN LEGNGVATDV PSATKRWGFR SGVPPKVVNY EAGEWAENCY NLEIKKPDGS ECLPAAPDGI RGFPRCRYVH KVSGTGPCAG DFAFHKEGAF FLYDRLASTV IYRGTTFAEG VVAFLILPQA KKDFFSSHPL REPVNATEDP SSGYYSTTIR YQATGFGTNE TEYLFEVDNL TYVQLESRFT PQFLLQLNET IYTSGKRSNT TGKLIWKVNP EIDTTIGEWA FWETKKNLTR KIRSEELSFT VVSTHHQDTG EESASSGKLG LITNTIAGVA GLITGGRRTR REAIVNAQPK CNPNLHYWTT QDEGAAIGLA WIPYFGPAAE GIYIEGLMHN QDGLICGLRQ LANETTQALQ LFLRATTELR TFSILNRKAI DFLLQRWGGT CHILGPDCCI EPADWTKNIT DKIDQIIHDF VDGSGYIPEA PRDGQAYVRK DGEWVLLSTF LGTHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC GGAAACCCGT CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG AGTCCGCATC GACCGGATCG GAAAACCTCT CGAGAAAGGC GTCTAACCAG TCACAGTCGC AAGGTAGGCT GAGCACCGTG GCGGGCGGCA GCGGGTGGCG GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA TTAAAGTAGG CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGCAG GCTTGAGATC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT ACTTCTGCGC TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GATCTGGCCA TACACTTGAG TGACAATGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA AACGGATCTC TAGCGAATTC GCCGCCACCA TGGGAATCCT GCCCTCCCCC GGAATGCCTG CCCTGCTGTC CCTCGTGTCC CTTCTGTCCG TGCTGCTGAT GGGTTGCGTG GCGGAAACTG GTAGAAGCAT CCCTCTCGGC GTGATCCACA ACAGCGCCCT CCAAGTGTCA GACGTGGACA AGCTCGTCTG CCGCGATAAG CTCAGCTCCA CCAACCAGCT GCGGTCGGTC GGTCTGAACC TGGAAGGGAA CGGGGTGGCC ACCGACGTGC CGTCGGCGAC CAAGCGCTGG GGCTTTAGGT CGGGAGTGCC GCCCAAGGTC GTGAACTACG AGGCCGGCGA ATGGGCCGAA AACTGCTACA ATCTGGAAAT CAAGAAGCCT GACGGGTCCG AGTGCTTGCC GGCGGCCCCC GACGGCATCC GCGGATTCCC GCGGTGTAGA TACGTGCACA AAGTGTCCGG CACCGGCCCT TGCGCCGGGG ATTTCGCCTT TCATAAGGAA GGCGCTTTCT TCCTGTACGA CCGACTGGCC TCTACCGTGA TCTATCGGGG AACTACTTTC GCCGAGGGCG TGGTCGCATT CCTCATCCTG CCACAAGCTA AAAAGGACTT CTTTTCCTCC CACCCGCTGC GCGAACCCGT GAACGCCACC GAGGACCCAT CGTCCGGATA CTACTCGACC ACCATCCGGT ACCAGGCCAC CGGTTTCGGG ACTAACGAAA CCGAGTACCT CTTCGAAGTG GATAACCTGA CCTACGTGCA GCTGGAGTCC AGATTCACCC CGCAATTCCT CCTGCAACTC AACGAAACCA TCTACACCTC CGGAAAGAGA AGCAACACGA CTGGGAAGCT GATTTGGAAA GTCAATCCCG AGATTGACAC CACTATCGGA GAGTGGGCAT TCTGGGAAAC CAAGAAGAAC TTGACCAGAA AGATCCGGAG CGAAGAACTG TCGTTCACTG TGGTGTCCAC CCACCACCAG GACACCGGAG AGGAATCAGC CTCATCCGGA AAACTTGGAC TGATTACTAA CACGATTGCT GGTGTCGCTG GTCTTATCAC TGGCGGACGC CGGACCCGGC GGGAAGCGAT AGTGAACGCC CAGCCTAAGT GCAACCCAAA CCTCCACTAC TGGACGACAC AGGACGAGGG TGCCGCCATC GGGCTGGCCT GGATTCCTTA CTTCGGACCG GCGGCAGAGG GAATCTATAT TGAGGGCCTG ATGCATAACC AGGACGGCCT GATCTGTGGG CTGCGGCAAT TGGCAAATGA GACTACCCAG GCGCTGCAGC TGTTCTTGCG GGCCACTACC GAGCTTAGGA CTTTCAGCAT CCTGAACCGC AAGGCCATTG ATTTCCTGCT GCAACGCTGG GGCGGAACTT GCCACATCCT CGGACCCGAC TGCTGTATCG AGCCGGCCGA TTGGACAAAG AACATCACCG ACAAGATTGA TCAGATTATT CACGACTTCG TGGACGGCAG CGGATACATC CCGGAAGCCC CCAGGGATGG CCAGGCCTAC GTGCGCAAGG ATGGCGAATG GGTCCTGCTC TCCACCTTCC TCGGCACCCA CCATCATCAC CACCACTGAT AAGGTTGAGC GGCCGCTCGA GGCCGGCAAG GCCAGATCCC CCGACCTCGA CCTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTGGT CGAGATCCCT CGGAGATCTC TAGCTAGAGG ATCGATCCCC GCCCCGGACG AACTAAACCT GACTACGACA TCTCTGCCCC TTCTTCGCGG GGCAGTGCAT GTAATCCCTT CAGTTGGTTG GTACAACTTG CCAACTGAAC CCTAAACGGG TAGCATATGC TTCCCGGGTA GTAGTATATA CTATCCAGAC TAACCCTAAT TCAATAGCAT ATGTTACCCA ACGGGAAGCA TATGCTATCG AATTAGGGTT AGTAAAAGGG TCCTAAGGAA CAGCGATGTA GGTGGGCGGG CCAAGATAGG GGCGCGATTG CTGCGATCTG GAGGACAAAT TACACACACT TGCGCCTGAG CGCCAAGCAC AGGGTTGTTG GTCCTCATAT TCACGAGGTC GCTGAGAGCA CGGTGGGCTA ATGTTGCCAT GGGTAGCATA TACTACCCAA ATATCTGGAT AGCATATGCT ATCCTAATCT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATCT GTATCCGGGT AGCATATGCT ATCCTAATAG AGATTAGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATATACT ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA TATGCTATCC TCATGATAAG CTGTCAAACA TGAGAATTAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCA
Details for EbzaA.19907.a.HE11.PD38471
| PURIFICATION DATe: | 6/10/2021 |
| CONCENTRATION: | 11.83mg/ml |
| OBSERVED MW: | 62kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM MES pH 5.2, 150mM NaCl |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 46 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
CID101539, PID9079-1, PUR2929
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGILPSPGMP ALLSLVSLLS VLLMGCVAET GRSIPLGVIH NSALQVSDVD KLVCRDKLSS TNQLRSVGLN LEGNGVATDV PSATKRWGFR SGVPPKVVNY EAGEWAENCY NLEIKKPDGS ECLPAAPDGI RGFPRCRYVH KVSGTGPCAG DFAFHKEGAF FLYDRLASTV IYRGTTFAEG VVAFLILPQA KKDFFSSHPL REPVNATEDP SSGYYSTTIR YQATGFGTNE TEYLFEVDNL TYVQLESRFT PQFLLQLNET IYTSGKRSNT TGKLIWKVNP EIDTTIGEWA FWETKKNLTR KIRSEELSFT VVSTHHQDTG EESASSGKLG LITNTIAGVA GLITGGRRTR REAIVNAQPK CNPNLHYWTT QDEGAAIGLA WIPYFGPAAE GIYIEGLMHN QDGLICGLRQ LANETTQALQ LFLRATTELR TFSILNRKAI DFLLQRWGGT CHILGPDCCI EPADWTKNIT DKIDQIIHDF VDGSGYIPEA PRDGQAYVRK DGEWVLLSTF LGTHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC GGAAACCCGT CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG AGTCCGCATC GACCGGATCG GAAAACCTCT CGAGAAAGGC GTCTAACCAG TCACAGTCGC AAGGTAGGCT GAGCACCGTG GCGGGCGGCA GCGGGTGGCG GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA TTAAAGTAGG CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGCAG GCTTGAGATC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT ACTTCTGCGC TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GATCTGGCCA TACACTTGAG TGACAATGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA AACGGATCTC TAGCGAATTC GCCGCCACCA TGGGAATCCT GCCCTCCCCC GGAATGCCTG CCCTGCTGTC CCTCGTGTCC CTTCTGTCCG TGCTGCTGAT GGGTTGCGTG GCGGAAACTG GTAGAAGCAT CCCTCTCGGC GTGATCCACA ACAGCGCCCT CCAAGTGTCA GACGTGGACA AGCTCGTCTG CCGCGATAAG CTCAGCTCCA CCAACCAGCT GCGGTCGGTC GGTCTGAACC TGGAAGGGAA CGGGGTGGCC ACCGACGTGC CGTCGGCGAC CAAGCGCTGG GGCTTTAGGT CGGGAGTGCC GCCCAAGGTC GTGAACTACG AGGCCGGCGA ATGGGCCGAA AACTGCTACA ATCTGGAAAT CAAGAAGCCT GACGGGTCCG AGTGCTTGCC GGCGGCCCCC GACGGCATCC GCGGATTCCC GCGGTGTAGA TACGTGCACA AAGTGTCCGG CACCGGCCCT TGCGCCGGGG ATTTCGCCTT TCATAAGGAA GGCGCTTTCT TCCTGTACGA CCGACTGGCC TCTACCGTGA TCTATCGGGG AACTACTTTC GCCGAGGGCG TGGTCGCATT CCTCATCCTG CCACAAGCTA AAAAGGACTT CTTTTCCTCC CACCCGCTGC GCGAACCCGT GAACGCCACC GAGGACCCAT CGTCCGGATA CTACTCGACC ACCATCCGGT ACCAGGCCAC CGGTTTCGGG ACTAACGAAA CCGAGTACCT CTTCGAAGTG GATAACCTGA CCTACGTGCA GCTGGAGTCC AGATTCACCC CGCAATTCCT CCTGCAACTC AACGAAACCA TCTACACCTC CGGAAAGAGA AGCAACACGA CTGGGAAGCT GATTTGGAAA GTCAATCCCG AGATTGACAC CACTATCGGA GAGTGGGCAT TCTGGGAAAC CAAGAAGAAC TTGACCAGAA AGATCCGGAG CGAAGAACTG TCGTTCACTG TGGTGTCCAC CCACCACCAG GACACCGGAG AGGAATCAGC CTCATCCGGA AAACTTGGAC TGATTACTAA CACGATTGCT GGTGTCGCTG GTCTTATCAC TGGCGGACGC CGGACCCGGC GGGAAGCGAT AGTGAACGCC CAGCCTAAGT GCAACCCAAA CCTCCACTAC TGGACGACAC AGGACGAGGG TGCCGCCATC GGGCTGGCCT GGATTCCTTA CTTCGGACCG GCGGCAGAGG GAATCTATAT TGAGGGCCTG ATGCATAACC AGGACGGCCT GATCTGTGGG CTGCGGCAAT TGGCAAATGA GACTACCCAG GCGCTGCAGC TGTTCTTGCG GGCCACTACC GAGCTTAGGA CTTTCAGCAT CCTGAACCGC AAGGCCATTG ATTTCCTGCT GCAACGCTGG GGCGGAACTT GCCACATCCT CGGACCCGAC TGCTGTATCG AGCCGGCCGA TTGGACAAAG AACATCACCG ACAAGATTGA TCAGATTATT CACGACTTCG TGGACGGCAG CGGATACATC CCGGAAGCCC CCAGGGATGG CCAGGCCTAC GTGCGCAAGG ATGGCGAATG GGTCCTGCTC TCCACCTTCC TCGGCACCCA CCATCATCAC CACCACTGAT AAGGTTGAGC GGCCGCTCGA GGCCGGCAAG GCCAGATCCC CCGACCTCGA CCTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTGGT CGAGATCCCT CGGAGATCTC TAGCTAGAGG ATCGATCCCC GCCCCGGACG AACTAAACCT GACTACGACA TCTCTGCCCC TTCTTCGCGG GGCAGTGCAT GTAATCCCTT CAGTTGGTTG GTACAACTTG CCAACTGAAC CCTAAACGGG TAGCATATGC TTCCCGGGTA GTAGTATATA CTATCCAGAC TAACCCTAAT TCAATAGCAT ATGTTACCCA ACGGGAAGCA TATGCTATCG AATTAGGGTT AGTAAAAGGG TCCTAAGGAA CAGCGATGTA GGTGGGCGGG CCAAGATAGG GGCGCGATTG CTGCGATCTG GAGGACAAAT TACACACACT TGCGCCTGAG CGCCAAGCAC AGGGTTGTTG GTCCTCATAT TCACGAGGTC GCTGAGAGCA CGGTGGGCTA ATGTTGCCAT GGGTAGCATA TACTACCCAA ATATCTGGAT AGCATATGCT ATCCTAATCT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATCT GTATCCGGGT AGCATATGCT ATCCTAATAG AGATTAGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATATACT ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA TATGCTATCC TCATGATAAG CTGTCAAACA TGAGAATTAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCA