EbzaA.17250.a
Minor nucleoprotein VP30
CENTER ID:
EbzaA.17250.a
ORGANISM:
Ebolavirus Zaire ebolavirus Mayinga-76
ASSOCIATED DISEASE:
Hemorrhagic fever
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
4
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIA
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
EbzaA.17250.a.ET11.GE00429
full length
info
3
135
EbzaA.17250.a.ET11.GE00429
EbzaA.17250.a.EW11.GE00428
full length
info
1
135
EbzaA.17250.a.EW11.GE00428
EbzaA.17250.a.EW12.GE00479
full length
info
1
135
EbzaA.17250.a.EW12.GE00479
EbzaA.17250.a.EW13.GE00480
full length
info
1
135
EbzaA.17250.a.EW13.GE00480
EbzaA.17250.a.EW14.GE00481
full length
info
1
135
EbzaA.17250.a.EW14.GE00481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
EbzaA.17250.a.EW12.PD00392
full length
info
1
135
PD00392
EbzaA.17250.a.EW12.PD00393
full length
info
1
135
PD00393
EbzaA.17250.a.EW13.PD00394
full length
info
1
135
PD00394
EbzaA.17250.a.EW13.PD00395
full length
info
1
135
PD00395
EbzaA.17250.a.EW14.PD00396
full length
info
1
135
PD00396
EbzaA.17250.a.EW14.PD00397
full length
info
1
135
PD00397
EbzaA.17250.a.EW11.PD00446
full length
info
1
135
PD00446
EbzaA.17250.a.EW16.PD00457
full length
info
1
135
PD00457
EbzaA.17250.a.EW19.PD00458
full length
info
1
135
PD00458
Structures
5DVW
DEPOSITED: 9/21/2015
DETERMINATION: XRay
CLONE: EbzaA.17250.a.EW11.GE00428
PROTEIN: EbzaA.17250.a.EW11.PD00370
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
[sequence withheld]
NT Sequence
[sequence withheld]
Details for EbzaA.17250.a.ET11.GE00429
HARVESTED ON:
3/11/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
17kDa
OBSERVED MW:
17kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW11.GE00428
HARVESTED ON:
3/17/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
26kDa
OBSERVED MW:
26kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW12.GE00479
HARVESTED ON:
8/26/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
26kDa
OBSERVED MW:
26kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Data Unavailable
SOLUBLE EXPRESSION LEVEL
Data Unavailable
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW13.GE00480
HARVESTED ON:
8/26/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
26kDa
OBSERVED MW:
26kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Data Unavailable
SOLUBLE EXPRESSION LEVEL
Data Unavailable
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW14.GE00481
HARVESTED ON:
8/26/2015
SEQUENCED ON:
5/4/2017
EXPECTED MW:
26kDa
OBSERVED MW:
26kDa
ANTIBIOTIC MARKER:
kanamycin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Data Unavailable
SOLUBLE EXPRESSION LEVEL
Data Unavailable
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW12.PD00392
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
9.53mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
22
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW12.PD00393
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
9.65mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
44
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW13.PD00394
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
10.82mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
33
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW13.PD00395
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
10.78mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
53
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW14.PD00396
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
9.42mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
10
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW14.PD00397
PURIFICATION DATe:
9/26/2015
CONCENTRATION:
9.45mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
10
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW11.PD00446
PURIFICATION DATe:
4/17/2016
CONCENTRATION:
19.98mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM Tris pH 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
93
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW16.PD00457
PURIFICATION DATe:
7/10/2016
CONCENTRATION:
19.51mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25mM TRIS pH 8.0, 200mM NaCl, 1mM TCEP, 1% glycerol.
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
36
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for EbzaA.17250.a.EW19.PD00458
PURIFICATION DATe:
7/13/2016
CONCENTRATION:
20.08mg/ml
OBSERVED MW:
26kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% glycerol
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
60
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable