EbreA.17250.a
Minor nucleoprotein VP30
| CENTER ID: | EbreA.17250.a |
| ORGANISM: | Ebolavirus Reston ebolavirus Reston-89 |
| ASSOCIATED DISEASE: | Hemorrhagic fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 4 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIA |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EbreA.17250.a.ET11.GE00432 | full length | 1 | 132 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EbreA.17250.a.EW11.PD00453 | full length | 1 | 109 |
Structures
3V7O
DEPOSITED: 12/21/2011
DETERMINATION: XRay
CLONE: EbreA.17250.a.D11.GE00001
PROTEIN: EbreA.17250.a.D11.PD00244
Publications by SSGCID
Structure of the Reston ebolavirus VP30 C-terminal domain.
Abendroth J, Atkins K, Barnes S, Clifton MC, Edwards TE, Grice R, Kirchdoerfer RN, Lorimer D, Moen S, Myler PJ, Raymond A, Saphire EO
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2014
volume 70, issue Pt 4, pages 457-60
PMID: 24699737; PMCID: PMC3976061
PDB:
3V7O
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|186539.27.CDS.6 |
| RefSeq: | NP_690585.1 |
| UniProt: | Q8JPX6 |
| ViPR: | link |
| ViPR: | link |
| ViPR: | link |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
[sequence withheld]
NT Sequence
[sequence withheld]
Details for EbreA.17250.a.ET11.GE00432
| HARVESTED ON: | 3/13/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 17kDa |
| OBSERVED MW: | 17kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGSSHHHHHH GSLEVLFQGP LTLAVLLQIA EHWATRDLRQ IEDSKLRALL TLCAVLTRKF SKSQLGLLCE THLRHEGLGQ DQADSVLEVY QRLHSDKGGN FEAALWQQWD RQSLIMFISA FLNIALQIPC ESSSVVVSGL ATLYPAQDNS T
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttagg aggtaaaaca tatgggttcg tcgcatcatc accatcacca tggtagcctg gaagtgctgt tccaaggccc gctcactctg gcagtgttac tgcagatcgc cgaacactgg gcaacccgcg acctgcgtca aatcgaagac tccaagttac gcgctctgct gaccctctgc gctgttctga cccgcaagtt tagcaaatct cagcttggcc tgctctgcga aactcacctg cgtcatgagg gcctcggtca ggatcaggct gacagcgttc tggaggtgta ccagcgtctg cactccgaca agggcggcaa cttcgaagcc gcactgtggc aacaatggga ccgccagagc ctcatcatgt ttatttccgc ttttctcaac atcgccctgc agattccttg cgaatcttcc tccgtagttg tctccggtct ggctaccctg taccctgctc aggataacag cacgtagtga ctcgagcccc ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgcc cctgagacgc gtcaatcgag ttcgtaccta agggcgacac cccctaatta gcccgggcga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga gtccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa caaggggtgt tatgagccat attcaggtat aaatgggctc gcgataatgt tcagaattgg ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaagc ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agatagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgcca gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tga
Details for EbreA.17250.a.EW11.PD00453
| PURIFICATION DATe: | 5/5/2016 |
| CONCENTRATION: | 8.525mg/ml |
| OBSERVED MW: | 23kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM Tris pH 8.5, 200mM NaCl, 1mM TCEP, 1% glycerol |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGLTLA VLLQIAEHWA TRDLRQIEDS KLRALLTLCA VLTRKFSKSQ LGLLCETHLR HEGLGQDQAD SVLEVYQRLH SDKGGNFEAA LWQQWDRQSL IMFISAFLNI ALQI
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAAACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAAACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGACT CACTCTGGCA GTGTTACTGC AGATCGCCGA ACACTGGGCA ACCCGCGACC TGCGTCAAAT CGAAGACTCC AAGTTACGCG CTCTGCTGAC CCTCTGCGCT GTTCTGACCC GCAAGTTTAG CAAATCTCAG CTTGGCCTGC TCTGCGAAAC TCACCTGCGT CATGAGGGCC TCGGTCAGGA TCAGGCTGAC AGCGTTCTGG AGGTGTACCA GCGTCTGCAC TCCGACAAGG GCGGCAACTT CGAAGCCGCA CTGTGGCAAC AATGGGACCG CCAGAGCCTC ATCATGTTTA TTTCCGCTTT TCTCAACATC GCCCTGCAGA TTTGATAACA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT