CyheA.18081.a

Tegument protein TRS1

CENTER ID: CyheA.18081.a
ORGANISM: Cytomegalovirus Human herpesvirus 5
ASSOCIATED DISEASE: CMV (herpes) infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CyheA.18081.a.A13.GE33873 full length 123 795
CyheA.18081.a.A14.GE33874 full length 305 795
CyheA.18081.a.A16.GE33876 full length 615 795
CyheA.18081.a.B21.GE40360 core dsRNA-binding domain 72 250
CyheA.18081.a.B41.GE40361 core dsRNA-binding domain, no N-term disorder 77 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CyheA.18081.a.MB31.PS38383 US22 core domain 82 351
External Resources
RESOURCE REFERENCE ID
UniProt: B9VXG6
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQRNGMSPR PPPLGRGRGA GGPSGVASSP SSCVPMGATS TAGTGASTAG SATPGHGVQR VEPRGPPGAP PGSGNNSNFW HGPERLLLSQ IPVERQALTE LEYQAMGAVW RAAFLANSTG RAMRKWSQRD AGTLLPLGRP YGFYARVTPR SQMNGVGATD LRQLSPRDAW IVLVATVVHE VDPAADPTVG DKAGHPEGLC AQDGLYLALG AGFRVFVYDL ANNTLILAAR DADEWFRHGA GEVVRVYRCN RLGVGTPRAT LLPQPALRQT LLRAEEATAL GRELRRRWAG TTVALQTPGR RLQPMVLLGA WQELAQYEPF ASAPHPASLL TAVRRHLNQR LCCGWLALGA VLPARWLGCA AGPATMTAGT TAMATGTTLP AGASGTETEA AGGDAPCAIA GAVGSAVTLP PQPYGAAGGS AVCVPNADTH AVVGTDAAAA AAAAPTVMVG PTAMAGPAAS GTVPRAMLVV VLDELGAVFG YCPLDGHVYP LAAELSHFLR AGVLGALALG RESAPAAEAA RRLLPELDRE QWERPRWDAL HLHPRAALWA REPHGQLAFL LRPGRGEAEV LTLATKHPAI CANVEDYLQD ARRRADAQAL GLDLATVVME AGGQMIHKKT KKPKGKEDES LMKGKHSRYT RPTEPPLTPQ ASLGRALRRD DEDWKPPRLP GEDSWYDLDE TFWVLGSNRK NDVYQRRWKK TVLRCGLEID RPMPTVPKGC RPQTFTHEGV QLMGGATQEP LDTGLYAPSH VTSAFVPSVY MPPTVPYPDP AARLCRDMRR VTFSNVATHY HYNAQ
NT Sequence
atggcccagc gcaacggcat gtcgccgcgc cccccgcccc tcggtcgcgg ccgcggtgcc ggagggcctt cgggggttgc ttcctctcct tcttcttgtg tgccgatggg agcgacgtca acggcgggaa ctggtgcaag tactgcgggt tcggcgacgc cgggccacgg cgtccaacgg gtagaacccc gcgggccgcc gggcgcccct ccgggtagcg gcaacaatag caacttttgg cacgggccgg agcgcctgtt gctgtctcag attccggtgg agcggcaggc gctgacggaa ctggaatacc aggccatggg cgccgtgtgg cgcgcggcgt ttctggccaa cagcacgggc cgcgccatgc gcaagtggtc gcagcgcgac gcgggcacgc tgctgccgct cggacggccg tacggattct acgcgcgggt gacgccgcgc agccagatga acggcgtggg cgcgacggac ctgcgtcagc tgtcgccgcg ggacgcgtgg atcgtgctgg tggcgaccgt ggtgcacgag gtagaccccg cggccgaccc gacggtgggc gacaaggccg gccatcccga gggtctgtgc gcgcaggacg gactgtacct ggcgctgggc gccggattcc gcgtgttcgt gtacgacctg gcgaacaaca cgctgatcct ggcggcgcgc gacgcggacg agtggtttcg gcacggcgcg ggcgaggtgg tgcgggtgta ccgctgcaac cggctgggcg tgggcacccc gcgcgcgacg ctgctgcctc agccggcgct tcgacagacc ttgctgcgcg ccgaggaggc gacggcgctc ggacgggagc tgcgccggcg gtgggccggc acgacggtgg cgctgcagac gccgggcagg cgactgcagc cgatggtact gctgggcgcg tggcaggagc tggcgcagta cgagccgttc gcgtcggcgc cgcaccccgc gtcgctgctg acggccgtgc gtcggcacct gaaccagcgt ctgtgctgcg gctggctggc gctgggcgcg gtgctgcccg cgcggtggct gggctgcgcg gcggggccgg cgacgatgac ggcggggacg acggcgatgg cgacggggac gacgttgccg gcgggggcga gcggcacgga gacggaggcc gccggcgggg acgcgccgtg cgcgatagcg ggagccgtgg ggtctgctgt gactttacct ccgcagccgt acggcgccgc cggcgggagc gcggtttgcg taccaaacgc ggacacgcac gcggtggtcg gaacggatgc ggcggcggcg gcagcggcgg cgccgacggt gatggtgggt ccgacggcga tggcgggtcc ggcggcgtcg gggaccgtgc cgcgcgccat gctggtggtg gtgctggacg agctgggcgc cgtgttcggg tactgcccgc tggacgggca cgtgtacccg ctggcggcgg agctgtcgca ctttctgcgc gcgggcgtgc tgggcgcgct ggcgctgggg cgcgagtcgg cgcccgccgc cgaggccgcg cggcggctgc tgcccgagct ggaccgcgag cagtgggagc ggccgcgctg ggacgcgctg cacctgcacc cgcgcgccgc gctgtgggcg cgcgagccgc acgggcagtt agcttttttg ctacgtccgg gccgcggcga ggctgaggtc ctcaccctag ccaccaaaca cccggccatc tgtgcaaatg tggaagacta cctgcaagac gctcgacgcc gcgccgacgc tcaagctctg ggactggact tggccacggt ggtgatggag gccggcggcc agatgataca caaaaagacc aaaaagccta aaggcaagga ggacgaatcg ctcatgaagg gaaaacactc gcgctacacc cgacccacag aaccaccgtt gactccccaa gccagtttgg gacgtgcttt gcgacgggac gatgaggatt ggaaaccgcc acgtctccct ggggaagact cctggtacga cttggacgag actttctggg ttctggggag taaccgtaaa aacgacgtgt atcaacgacg ttggaagaaa accgtgttac gctgtggttt ggagattgat cgtcccatgc caacggtccc caagggctgc cgcccgcaga cttttactca tgagggcgtt cagttgatgg gtggtgctac tcaggaaccg ctggatactg gactatatgc tccgtctcac gttacttctg cttttgttcc atctgtgtac atgcctccca ccgtacccta ccccgacccc gctgccagat tgtgccgcga catgcgacgc gtcacgtttt ctaatgtagc cacacactac cattacaatg ctcaataa
Details for CyheA.18081.a.A13.GE33873
HARVESTED ON: 10/15/2011
SEQUENCED ON: 10/21/2011
EXPECTED MW: 74kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 54
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRKWSQRDA GTLLPLGRPY GFYARVTPRS QMNGVGATDL RQLSPRDAWI VLVATVVHEV DPAADPTVGD KAGHPEGLCA QDGLYLALGA GFRVFVYDLA NNTLILAARD ADEWFRHGAG EVVRVYRCNR LGVGTPRATL LPQPALRQTL LRAEEATALG RELRRRWAGT TVALQTPGRR LQPMVLLGAW QELAQYEPFA SAPHPASLLT AVRRHLNQRL CCGWLALGAV LPARWLGCAA GPATMTAGTT AMATGTTLXA GXXXTETEAX GGXAXXAIAX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX AXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagaaa gtggtcccag cgtgacgccg gtactctgct cccgctgggt cgtccgtacg gcttctacgc acgtgttact cctagatctc agatgaacgg cgtaggtgct actgacctgc gccagctctc tccgagagac gcatggattg ttctggtggc gactgtagtt catgaagttg acccggctgc tgacccgacc gtgggtgaca aagctggcca cccggaaggt ctgtgcgcgc aggacggcct gtacctggcg ttaggcgccg gctttagagt atttgtctac gatcttgcga acaacaccct gatcctggcg gcgcgcgacg cagacgaatg gttccgtcac ggcgcaggtg aggtagttag agtataccgt tgcaaccgtc tgggcgttgg tactccgcgt gctaccctgc tgccgcagcc agctctgcgt caaaccctgc tgcgtgcgga agaagcaacc gcgttaggcc gtgaactgcg tcgccgttgg gcgggtacta ccgttgctct gcagactcct ggccgtcgtc tgcagcctat ggtactgtta ggcgcttggc aggaactcgc gcagtatgaa ccgttcgctt ctgcgccgca cccggcatcc ctgctgaccg ctgtacgtcg tcacctgaac cagcgtctgt gctgcggctg gttagctctg ggtgccgttc tgccggctag atggttaggc tgcgctgctg gcccagcaac catgaccgct ggcactactg ctatggcaac tggtactacc ctgncngccg gtgnttncng tactgaaact gaagctgnng gtggcnatgc ancgnncgct atagctgnnn tntngcngcn cantgannnt nnncncannn tnntggngca ncnnngancn cntnnnnnnn nnnntnannn ccnnnnnnnn nnnnncnatg ctncngnngn gnnncannnn nacnnannnn nnnnancnnn ngnnngnnnn nnntnnnnnn ncnnnncnnn nnnnnnnnnn nnnnnnnann nnnnnnnnnt nnnnnnnnnn nnnnnnncnn nnnnnnannn nnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKWSQRDA GTLLPLGRPY GFYARVTPRS QMNGVGATDL RQLSPRDAWI VLVATVVHEV DPAADPTVGD KAGHPEGLCA QDGLYLALGA GFRVFVYDLA NNTLILAARD ADEWFRHGAG EVVRVYRCNR LGVGTPRATL LPQPALRQTL LRAEEATALG RELRRRWAGT TVALQTPGRR LQPMVLLGAW QELAQYEPFA SAPHPASLLT AVRRHLNQRL CCGWLALGAV LPARWLGCAA GPATMTAGTT AMATGTTLPA GASGTETEAA GGDAPCAIAG AVGSAVTLPP QPYGAAGGSA VCVPNADTHA VVGTDAAAAA AAAPTVMVGP TAMAGPAASG TVPRAMLVVV LDELGAVFGY CPLDGHVYPL AAELSHFLRA GVLGALALGR ESAPAAEAAR RLLPELDREQ WERPRWDALH LHPRAALWAR EPHGQLAFLL RPGRGEAEVL TLATKHPAIC ANVEDYLQDA RRRADAQALG LDLATVVMEA GGQMIHKKTK KPKGKEDESL MKGKHSRYTR PTEPPLTPQA SLGRALRRDD EDWKPPRLPG EDSWYDLDET FWVLGSNRKN DVYQRRWKKT VLRCGLEIDR PMPTVPKGCR PQTFTHEGVQ LMGGATQEPL DTGLYAPSHV TSAFVPSVYM PPTVPYPDPA ARLCRDMRRV TFSNVATHYH YNAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaaagtgg tcccagcgtg acgccggtac tctgctcccg ctgggtcgtc cgtacggctt ctacgcacgt gttactccta gatctcagat gaacggcgta ggtgctactg acctgcgcca gctctctccg agagacgcat ggattgttct ggtggcgact gtagttcatg aagttgaccc ggctgctgac ccgaccgtgg gtgacaaagc tggccacccg gaaggtctgt gcgcgcagga cggcctgtac ctggcgttag gcgccggctt tagagtattt gtctacgatc ttgcgaacaa caccctgatc ctggcggcgc gcgacgcaga cgaatggttc cgtcacggcg caggtgaggt agttagagta taccgttgca accgtctggg cgttggtact ccgcgtgcta ccctgctgcc gcagccagct ctgcgtcaaa ccctgctgcg tgcggaagaa gcaaccgcgt taggccgtga actgcgtcgc cgttgggcgg gtactaccgt tgctctgcag actcctggcc gtcgtctgca gcctatggta ctgttaggcg cttggcagga actcgcgcag tatgaaccgt tcgcttctgc gccgcacccg gcatccctgc tgaccgctgt acgtcgtcac ctgaaccagc gtctgtgctg cggctggtta gctctgggtg ccgttctgcc ggctagatgg ttaggctgcg ctgctggccc agcaaccatg accgctggca ctactgctat ggcaactggt actaccctgc cggccggtgc ttccggtact gaaactgaag ctgcgggtgg cgatgcaccg tgcgctatag ctggcgctgt tggcagcgca gtgactctgc cgccgcagcc atatggtgca gcaggcggta gcgccgtttg cgttcctaac gctgacaccc acgctgtagt tggtaccgat gctgcagcgg ctgcggctgc agctccgacc gtaatggtag gcccgaccgc aatggctggt ccggctgcta gcggtaccgt tcctcgtgct atgctggttg tagtgctgga tgaattaggc gctgtatttg gctactgccc gctggacggc cacgtatacc cgctggctgc tgaactttct cacttcctgc gcgctggtgt cctgggtgct ctggcattag gccgcgaatc tgcgccggca gctgaagctg cgcgtcgtct cctgcctgaa ctggaccgtg aacagtggga acgtccgcgt tgggacgctc tgcacctgca cccgcgcgcg gctctgtggg cccgtgaacc tcacggccag ttagctttcc tgctgagacc gggccgtggc gaagcggaag ttctgacctt agccactaaa cacccggcta tctgcgctaa cgttgaagac tacctgcagg acgcacgtcg ccgtgctgac gctcaggctt taggcctcga tctggctacc gtcgtaatgg aagccggcgg tcagatgatc cacaagaaga ctaagaagcc taaaggcaaa gaagatgaat ctctgatgaa gggcaaacac tctcgttata cacgcccgac cgaaccgccg ctgactccgc aggctagctt aggccgtgct ctccgtcgtg acgacgaaga ctggaaaccg ccgcgcctgc cgggtgaaga tagctggtac gacctggacg agactttctg ggttttaggc tccaaccgta agaacgacgt ttaccagcgt cgttggaaga agaccgttct gagatgcggt ctcgaaatcg accgtccgat gccgaccgtg cctaaaggtt gccgtccgca gacctttacc catgaaggcg ttcagctgat gggtggcgct acccaggaac cgctggacac cggtctgtac gctccatccc acgtaacctc cgcattcgtt ccgagcgttt acatgccacc gacggtaccg tacccggatc cggcagctag actgtgccgt gacatgcgcc gtgtaacctt tagcaacgtt gcaacccact atcactacaa cgcacagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for CyheA.18081.a.A14.GE33874
HARVESTED ON: 10/15/2011
SEQUENCED ON: 10/21/2011
EXPECTED MW: 54kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 60
Validated AA Sequence
MGTLEAQTQG PGSMVLLGAW QELAQYEPFA SAPHPASLLT AVRRHLNQRL CCGWLALGAV LPARWLGCAA GPATMTAGTT AMATGTTLPA GASGTETEAA GGDAPCAIAG AVGSAVTLPP QPYGAAGGSA VCVPNADTHA VVGTDAAAAA AAAPTVMVGP TAMAGPAASG TVPRAMLVVV LDELGAVFGY CPLDGHVYPL AAELSHFLRA GVLGALALGR ESAPAAEAAR RLLPELDREQ WERPRWDALH LHPRAALWAR EPHGQLAFLL RPGRGXXEVL TLATKHXXIC AXXXXYLXXX XXXXXXXA
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtactg ttaggcgctt ggcaggaact cgcgcagtat gaaccgttcg cttctgcgcc gcacccggca tccctgctga ccgctgtacg tcgtcacctg aaccagcgtc tgtgctgcgg ctggttagct ctgggtgccg ttctgccggc tagatggtta ggctgcgctg ctggcccagc aaccatgacc gctggcacta ctgctatggc aactggtact accctgccgg ccggtgcttc cggtactgaa actgaagctg cgggtggcga tgcaccgtgc gctatagctg gcgctgttgg cagcgcagtg actctgccgc cgcagccata tggtgcagca ggcggtagcg ccgtttgcgt tcctaacgct gacacccacg ctgtagttgg taccgatgct gcagcggctg cggctgcagc tccgaccgta atggtaggcc cgaccgcaat ggctggtccg gctgctagcg gtaccgttcc tcgtgctatg ctggttgtag tgctggatga attaggcgct gtatttggct actgcccgct ggacggccac gtatacccgc tggctgctga actttctcac ttcctgcgcg ctggtgtcct gggtgctctg gcattaggcc gcgaatctgc gccggcagct gaagctgcgc gtcgtctcct gcctgaactg gaccgtgaac agtgggaacg tccgcgttgg gacgctctgc acctgcaccc gcgcgcggct ctgtgggccc gtgaacctca cggccagtta gctttcctgc tgagaccggg ccgtggcgan ncngaagttc tgaccttagc cactaaacac nnnnctatct gcgctnnnnn tgnnnnctac ctgnnnnnnn nnnnnnnnnn nnctgannnt nangcttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLGAWQE LAQYEPFASA PHPASLLTAV RRHLNQRLCC GWLALGAVLP ARWLGCAAGP ATMTAGTTAM ATGTTLPAGA SGTETEAAGG DAPCAIAGAV GSAVTLPPQP YGAAGGSAVC VPNADTHAVV GTDAAAAAAA APTVMVGPTA MAGPAASGTV PRAMLVVVLD ELGAVFGYCP LDGHVYPLAA ELSHFLRAGV LGALALGRES APAAEAARRL LPELDREQWE RPRWDALHLH PRAALWAREP HGQLAFLLRP GRGEAEVLTL ATKHPAICAN VEDYLQDARR RADAQALGLD LATVVMEAGG QMIHKKTKKP KGKEDESLMK GKHSRYTRPT EPPLTPQASL GRALRRDDED WKPPRLPGED SWYDLDETFW VLGSNRKNDV YQRRWKKTVL RCGLEIDRPM PTVPKGCRPQ TFTHEGVQLM GGATQEPLDT GLYAPSHVTS AFVPSVYMPP TVPYPDPAAR LCRDMRRVTF SNVATHYHYN AQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtactgtta ggcgcttggc aggaactcgc gcagtatgaa ccgttcgctt ctgcgccgca cccggcatcc ctgctgaccg ctgtacgtcg tcacctgaac cagcgtctgt gctgcggctg gttagctctg ggtgccgttc tgccggctag atggttaggc tgcgctgctg gcccagcaac catgaccgct ggcactactg ctatggcaac tggtactacc ctgccggccg gtgcttccgg tactgaaact gaagctgcgg gtggcgatgc accgtgcgct atagctggcg ctgttggcag cgcagtgact ctgccgccgc agccatatgg tgcagcaggc ggtagcgccg tttgcgttcc taacgctgac acccacgctg tagttggtac cgatgctgca gcggctgcgg ctgcagctcc gaccgtaatg gtaggcccga ccgcaatggc tggtccggct gctagcggta ccgttcctcg tgctatgctg gttgtagtgc tggatgaatt aggcgctgta tttggctact gcccgctgga cggccacgta tacccgctgg ctgctgaact ttctcacttc ctgcgcgctg gtgtcctggg tgctctggca ttaggccgcg aatctgcgcc ggcagctgaa gctgcgcgtc gtctcctgcc tgaactggac cgtgaacagt gggaacgtcc gcgttgggac gctctgcacc tgcacccgcg cgcggctctg tgggcccgtg aacctcacgg ccagttagct ttcctgctga gaccgggccg tggcgaagcg gaagttctga ccttagccac taaacacccg gctatctgcg ctaacgttga agactacctg caggacgcac gtcgccgtgc tgacgctcag gctttaggcc tcgatctggc taccgtcgta atggaagccg gcggtcagat gatccacaag aagactaaga agcctaaagg caaagaagat gaatctctga tgaagggcaa acactctcgt tatacacgcc cgaccgaacc gccgctgact ccgcaggcta gcttaggccg tgctctccgt cgtgacgacg aagactggaa accgccgcgc ctgccgggtg aagatagctg gtacgacctg gacgagactt tctgggtttt aggctccaac cgtaagaacg acgtttacca gcgtcgttgg aagaagaccg ttctgagatg cggtctcgaa atcgaccgtc cgatgccgac cgtgcctaaa ggttgccgtc cgcagacctt tacccatgaa ggcgttcagc tgatgggtgg cgctacccag gaaccgctgg acaccggtct gtacgctcca tcccacgtaa cctccgcatt cgttccgagc gtttacatgc caccgacggt accgtacccg gatccggcag ctagactgtg ccgtgacatg cgccgtgtaa cctttagcaa cgttgcaacc cactatcact acaacgcaca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for CyheA.18081.a.A16.GE33876
HARVESTED ON: 10/15/2011
SEQUENCED ON: 10/21/2011
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIHKKTKKP KGKEDESLMK GKHSRYTRPT EPPLTPQASL GRALRRDDED WKPPRLPGED SWYDLDETFW VLGSNRKNDV YQRRWKKTVL RCGLEIDRPM PTVPKGCRPQ TFTHEGVQLM GGATQEPLDT GLYAPSHVTS AFVPSVYMPP TVPYPDPAAR LCRDMRRVTF SNVATHYHYN AQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatcc acaagaagac taagaagcct aaaggcaaag aagatgaatc tctgatgaag ggcaaacact ctcgttatac acgcccgacc gaaccgccgc tgactccgca ggctagctta ggccgtgctc tccgtcgtga cgacgaagac tggaaaccgc cgcgcctgcc gggtgaagat agctggtacg acctggacga gactttctgg gttttaggct ccaaccgtaa gaacgacgtt taccagcgtc gttggaagaa gaccgttctg agatgcggtc tcgaaatcga ccgtccgatg ccgaccgtgc ctaaaggttg ccgtccgcag acctttaccc atgaaggcgt tcagctgatg ggtggcgcta cccaggaacc gctggacacc ggtctgtacg ctccatccca cgtaacctcc gcattcgttc cgagcgttta catgccaccg acggtaccgt acccggatcc ggcagctaga ctgtgccgtg acatgcgccg tgtaaccttt agcaacgttg caacccacta tcactacaac gcacagtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIHKKTKKP KGKEDESLMK GKHSRYTRPT EPPLTPQASL GRALRRDDED WKPPRLPGED SWYDLDETFW VLGSNRKNDV YQRRWKKTVL RCGLEIDRPM PTVPKGCRPQ TFTHEGVQLM GGATQEPLDT GLYAPSHVTS AFVPSVYMPP TVPYPDPAAR LCRDMRRVTF SNVATHYHYN AQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatccacaag aagactaaga agcctaaagg caaagaagat gaatctctga tgaagggcaa acactctcgt tatacacgcc cgaccgaacc gccgctgact ccgcaggcta gcttaggccg tgctctccgt cgtgacgacg aagactggaa accgccgcgc ctgccgggtg aagatagctg gtacgacctg gacgagactt tctgggtttt aggctccaac cgtaagaacg acgtttacca gcgtcgttgg aagaagaccg ttctgagatg cggtctcgaa atcgaccgtc cgatgccgac cgtgcctaaa ggttgccgtc cgcagacctt tacccatgaa ggcgttcagc tgatgggtgg cgctacccag gaaccgctgg acaccggtct gtacgctcca tcccacgtaa cctccgcatt cgttccgagc gtttacatgc caccgacggt accgtacccg gatccggcag ctagactgtg ccgtgacatg cgccgtgtaa cctttagcaa cgttgcaacc cactatcact acaacgcaca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for CyheA.18081.a.B21.GE40360
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGS GNNSNFWHGP ERLLLSQIPV ERQALTELEY QAMGAVWRAA FLANSTGRAM RKWSQRDAGT LLPLGRPYGF YARVTPRSQM NGVGATDLRQ LSPRDAWIVL VATVVHEVDP AADPTVGDKA GHPEGLCAQD GLYLALGAGF RVFVYDLANN TLILAARDAD EWFRHGAGEV VRVYRCN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggatccg gtaataattc taatttttgg catggtccgg aacgtttact gttaagtcag attccggttg aacgtcaggc actgaccgaa ctcgagtatc aagcgatggg tgcagtgtgg cgtgcagcgt ttctggcgaa tagtaccggt cgtgcaatgc gtaaatggag ccagcgtgat gcgggtaccc tgctgccact gggtcgtccg tatggttttt atgcacgtgt taccccgcgt tctcagatga atggtgtggg tgcgacagat ctgcgtcaac tgagcccacg tgatgcatgg attgttttag tggcgaccgt tgtgcatgaa gtcgacccgg cagccgatcc gaccgtgggt gataaagcgg gtcatccaga aggcctgtgt gcacaggatg gtttatatct ggcgctgggc gcgggttttc gtgtttttgt gtatgatctg gcaaataata ccttaattct ggcagcgcgt gatgcggatg aatggtttcg tcatggcgcc ggtgaagttg tgcgtgttta tcgttgtaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for CyheA.18081.a.B41.GE40361
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 20kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSN FWHGPERLLL SQIPVERQAL TELEYQAMGA VWRAAFLANS TGRAMRKWSQ RDAGTLLPLG RPYGFYARVT PRSQMNGVGA TDLRQLSPRD AWIVLVATVV HEVDPAADPT VGDKAGHPEG LCAQDGLYLA LGAGFRVFVY DLANNTLILA ARDADEWFRH GAGEVVRVYR CN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctaatt tttggcatgg tccggaacgt ttactgttaa gtcagattcc ggttgaacgt caggcactga ccgaactcga gtatcaagcg atgggtgcag tgtggcgtgc agcgtttctg gcgaatagta ccggtcgtgc aatgcgtaaa tggagccagc gtgatgcggg taccctgctg ccactgggtc gtccgtatgg tttttatgca cgtgttaccc cgcgttctca gatgaatggt gtgggtgcga cagatctgcg tcaactgagc ccacgtgatg catggattgt tttagtggcg accgttgtgc atgaagtcga cccggcagcc gatccgaccg tgggtgataa agcgggtcat ccagaaggcc tgtgtgcaca ggatggttta tatctggcgc tgggcgcggg ttttcgtgtt tttgtgtatg atctggcaaa taatacctta attctggcag cgcgtgatgc ggatgaatgg tttcgtcatg gcgccggtga agttgtgcgt gtttatcgtt gtaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CyheA.18081.a.MB31.PS38383
PURIFICATION DATe: 8/22/2017
CONCENTRATION: 11.16mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: Rosetta 2(DE3) Singles(TM) Competent Cells
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 68
Protocol Notes
notes unavailable
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSGP ERLLLSQIPV ERQALTELEY QAMGAVWRAA FLANSTGRAM RKWSQRDAGT LLPLGRPYGF YARVTPRSQM NGVGATDLRQ LSPRDAWIVL VATVVHEVDP AADPTVGDKA GHPEGLCAQD GLYLALGAGF RVFVYDLANN TLILAARDAD EWFRHGAGEV VRVYRCNRLG VGTPRATLLP QPALRQTLLR AEEATALGRE LRRRWAGTTV ALQTPGRRLQ PMVLLGAWQE LAQYEPFASA PHPASLLTAV RRHLNQRLCC GWLALGAV
Validated NT Sequence
tgcnnnnngg ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cnnnnnnaac gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaac aacaataaca acaacctcgg gatcgaggga aggatttcag aaggtaccct ggaagctcag acccagggtc ctggttcggg tcctgaacgt cttctgctct cccaaatccc agtcgaacgc caggctttga ctgaacttga atatcaggca atgggtgctg tttggcgtgc tgctttctta gcgaatagca ctggccgtgc gatgagaaag tggtcccagc gtgacgccgg tactctgctc ccgctgggtc gtccgtacgg cttctacgca cgtgttactc ctagatctca gatgaacggc gtaggtgcta ctgacctgcg ccagctctct ccgagagacg catggattgt tctggtggcg actgtagttc atgaagttga cccggctgct gacccgaccg tgggtgacaa agctggccac ccggaaggtc tgtgcgcgca ggacggcctg tacctggcgt taggcgccgg ctttagagta tttgtctacg atcttgcgaa caacaccctg atcctggcgg cgcgcgacgc agacgaatgg ttccgtcacg gcgcaggtga ggtagttaga gtataccgtt gcaaccgtct gggcgttggt actccgcgtg ctaccctgct gccgcagcca gctctgcgtc aaaccctgct gcgtgcggaa gaagcaaccg cgttaggccg tgaactgcgt cgccgttggg cgggtactac cgttgctctg cagactcctg gccgtcgtct gcagcctatg gtactgttag gcgcttggca ggaactcgcg cagtatgaac cgttcgcttc tgcgccgcac ccggcatccc tgctgaccgc tgtacgtcgt cacctgaacc agcgtctgtg ctgcggctgg ttagctctgg gtgccgttta a
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS GPERLLLSQI PVERQALTEL EYQAMGAVWR AAFLANSTGR AMRKWSQRDA GTLLPLGRPY GFYARVTPRS QMNGVGATDL RQLSPRDAWI VLVATVVHEV DPAADPTVGD KAGHPEGLCA QDGLYLALGA GFRVFVYDLA NNTLILAARD ADEWFRHGAG EVVRVYRCNR LGVGTPRATL LPQPALRQTL LRAEEATALG RELRRRWAGT TVALQTPGRR LQPMVLLGAW QELAQYEPFA SAPHPASLLT AVRRHLNQRL CCGWLALGAV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAACAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGGGCC GGAGCGCCTG TTGCTGTCTC AGATTCCGGT GGAGCGGCAG GCGCTGACGG AACTGGAATA CCAGGCCATG GGCGCCGTGT GGCGCGCGGC GTTTCTGGCC AACAGCACGG GCCGCGCCAT GCGCAAGTGG TCGCAGCGCG ACGCGGGCAC GCTGCTGCCG CTCGGACGGC CGTACGGATT CTACGCGCGG GTGACGCCGC GCAGCCAGAT GAACGGCGTG GGCGCGACGG ACCTGCGTCA GCTGTCGCCG CGGGACGCGT GGATCGTGCT GGTGGCGACC GTGGTGCACG AGGTAGACCC CGCGGCCGAC CCGACGGTGG GCGACAAGGC CGGCCATCCC GAGGGTCTGT GCGCGCAGGA CGGACTGTAC CTGGCGCTGG GCGCCGGATT CCGCGTGTTC GTGTACGACC TGGCGAACAA CACGCTGATC CTGGCGGCGC GCGACGCGGA CGAGTGGTTT CGGCACGGCG CGGGCGAGGT GGTGCGGGTG TACCGCTGCA ACCGGCTGGG CGTGGGCACC CCGCGCGCGA CGCTGCTGCC TCAGCCGGCG CTTCGACAGA CCTTGCTGCG CGCCGAGGAG GCGACGGCGC TCGGACGGGA GCTGCGCCGG CGGTGGGCCG GCACGACGGT GGCGCTGCAG ACGCCGGGCA GGCGACTGCA GCCGATGGTA CTGCTGGGCG CGTGGCAGGA GCTGGCGCAG TACGAGCCGT TCGCGTCGGC GCCGCACCCC GCGTCGCTGC TGACGGCCGT GCGTCGGCAC CTGAACCAGC GTCTGTGCTG CGGCTGGCTG GCGCTGGGCG CGGTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA