CrpaA.19515.c

Phospodiesterase 5 (PDE5) analog; phosphdiesterase, putative

CENTER ID: CrpaA.19515.c
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.19515.c.B2.GE41009 PDE domain 857 1193
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd6_4020
RefSeq: XP_001388345.1
UniProt: A3FPW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFLRKRITRL DSNEKKYSIE EILNGITYGK IKKFSLGWNL WLGITISCIV LIWTAIYASR LLNIAHKLKN NDKLFDEIKF DEQLTIYGYN IGGLSLSILA FCKMESSPTL HNIGIRLGLS NQILKQRYML RNAIEKLNEI KYPIDKLDQK TIIEEMIENT TQLLKELVPL HFALEDQPSP ISPAPPPFYV PYRTNITESE IRNSTESDST TFLSAKYLCN NTEYISETSN SLRYKALNIS NPAKLFDLHY LNAYFKQSDI IKFQSTNRIL IFHFIVVIFV VIITLIVFYF GSNDHFQKKN RLKMMNICKK QIIDIFDQIL NVDKANKSLI RDKFKPVSKI FENLKYVCDG IVIEYGKDAQ NDIIFSLETL SKVIHEFQTC VELAQKFCDI LDNEININKK NTKEPIKIHE IIEQDIQNNN ITGNSHHFSD KVPNEIYLYS GKQEFNDIIN SVVNVLSPNL GKTIFWDIIQ VGDHSLIPNS MVNTIGEVST GVKQVIDDNW NIISSNQEEI REYSFLTIDI SPCMIFKNIH NEKKAEDIKV EENNEQYEVN CLLEALNCVT RSFHLLIAKR MANCVFGGVL ICHGNFGDQA REFQVNELNY IRDITGNTLI IPIKKNQKNL LKSNSLIKSL SNIINPDVKI YTSGLDFKVS TIIKNISKIL NIPHLEIECW VLQEKNDLVD YLLDKPGYWF IDTSIIHLIS SNSKYIINLY SQNYYLDNNI DVILLTTIGK VEVISEYNND INLNQFYYSL KYFLSGKSFP VKQSPSKTHI IQEKKINCDY RQIIRLLNRP ISEINENLFR DIGNMSQISG KDDSLIGSSY IKLVDIRELY CKQKMNKNSG KLKSFNDEKW DIRKPEILSK EWYNIDLLEL SIDEIKEIAE FLIYYRFREV IRDKDDYDDD VLSWQINKRK IKNFVSELIK YYHEENPFHN FHHAVSISIT LNNWLDKPSV RHYVNSLEAY CLIISSLSHD LDHPGLTNDM IKELNILSRK VLQEFTTDEQ FFEILQNNTL STLKIYNNFS VLENHHSSLL FVILNDEASN IMPPTTSKYY SELHKMMINS ILFTDMFQHK YLRDLVLNFN KNYSSNSRSI EVEAQKKKLY LLILLHSVDI SNPLMNTNIY LKWSRLVYEE LENQKKLKLM LNISLNDTTD TYDYTKLQTS FISSICIPYF QALLKFDPEI VQECIQNLYF NNEYFAKTGQ KTRTNK
NT Sequence
atgtttttaa gaaagcgaat aacaagatta gatagcaatg agaagaagta tagcatagaa gaaatactta atggaataac atatgggaaa ataaagaaat tttctttagg atggaactta tggttgggaa ttacaatatc ttgtattgta ctgatttgga cagcaatata tgcctcaaga ttgttaaata ttgcgcataa gttaaagaat aatgataaat tatttgatga aataaagttt gatgaacagc taacaattta tggatataac ataggaggtt taagtttatc tatattagct ttttgtaaga tggaaagcag tcccactttg cacaatattg gaataagact aggattatct aatcagattt tgaagcaaag atatatgcta agaaatgcaa tagaaaaatt gaatgaaata aaatatccaa tagataaatt agatcagaag acaataatag aagagatgat tgaaaataca acacaacttc taaaagagtt agtacctttg cattttgctc ttgaagatca gccaagtcca atttctccag ctcctcctcc attctatgtt ccttatagaa caaatattac agaaagtgaa ataagaaatt ctacagaaag tgactcaact acatttttgt cagctaaata tttatgtaac aacacggaat atatttcaga aacatcaaat tctctcagat ataaggcatt gaatataagt aacccagcca aattatttga tttgcattat ctgaatgcat actttaagca aagtgacata attaaatttc agtctacaaa caggatattg attttccact ttattgtcgt tatatttgtg gtaattataa ctttgatagt tttctacttt ggctcaaatg atcactttca gaagaaaaat cgacttaaaa tgatgaatat ttgtaaaaaa caaattattg atatctttga tcaaatactt aatgtggata aagccaataa gtctttaatt cgagataagt ttaagcctgt ttctaagata tttgagaatt taaagtatgt atgtgatgga attgtgatag aatatggtaa agatgctcag aatgatatta tattttcttt agaaacttta tcaaaggtta ttcatgaatt tcaaacttgt gttgaactag ctcaaaagtt ttgtgatatc ttggataatg aaattaatat aaacaaaaaa aacacaaaag agcctattaa aatacatgaa attattgaac aagacatcca aaataataat attactggta atagccacca tttctcagat aaagttccaa atgaaattta tttatactct ggtaaacaag aatttaatga cattattaac tcagtggtta atgttttaag tccaaatctt ggtaagacta ttttttggga tattattcag gttggtgatc attcacttat tccaaattct atggttaata ctattggaga agttagtaca ggcgtcaagc aagttattga tgataactgg aatataatat cttctaatca agaagaaatt agggaatatt cttttctgac aattgatatt tctccttgta tgatctttaa gaatattcat aatgaaaaaa aagcagaaga tatcaaagtt gaggaaaata atgaacaata tgaagttaat tgccttctag aggcattgaa ttgtgtaaca agaagtttcc atttattgat tgctaaaagg atggcaaatt gtgtttttgg aggggttttg atttgccatg gaaattttgg agatcaagcg agagagtttc aagtcaatga acttaattac attagagata ttactggaaa tactttaatt attccaataa agaagaatca aaagaactta ttaaaaagta attcactaat aaaaagcttg agtaatatta ttaatccaga tgtgaaaata tatacaagtg gtttagactt taaggtttca acaatcataa aaaatatttc caaaatctta aatattccac atttggaaat tgaatgttgg gttctacaag aaaaaaatga tttagttgac tatttattgg ataaaccagg atattggttt attgatactt caatcattca tttaatcagt agtaattcca agtatatcat taatttatat agccaaaatt actatttgga caataacatt gacgtaatac ttttaaccac aattggcaag gttgaagtta tctctgaata taacaatgac ataaatttga accagtttta ttattcatta aaatattttc taagtggcaa atcatttcca gttaaacaaa gcccatcaaa aactcatatt atacaagaaa agaagataaa ttgtgattat aggcaaatta ttagattatt gaacagacca atttctgaaa ttaatgagaa cttatttaga gatattggta atatgagcca aatctctgga aaggatgatt cattgattgg aagcagttat attaaactgg ttgatattag agagttatat tgtaaacaaa agatgaataa gaatagtggt aaacttaaaa gttttaatga tgagaaatgg gacattagaa aaccagaaat actttccaag gaatggtata atatcgactt gcttgaatta agtatagatg aaattaaaga aattgcagaa tttttaatct attacagatt tagagaagta atacgtgata aagacgatta tgatgatgat gttttgtctt ggcaaataaa caaaagaaaa attaaaaact ttgtttcaga gttaataaaa tattaccatg aagaaaatcc ttttcataat tttcatcatg ctgtaagtat ttccataaca ctaaataatt ggctagacaa accttctgtg agacattatg taaattcatt agaagcttat tgtttaatca tttcatctct ttcacatgat ttggatcatc caggattaac taatgatatg attaaagagt tgaatattct ctcaagaaaa gtattacaag aatttacaac agatgaacag ttttttgaaa ttttacaaaa caatactctc tcaacactaa aaatatataa taatttttca gtattggaaa atcatcattc ttctctctta tttgtaatat taaatgatga agcttccaat attatgcctc caactacttc caaatattat tcagaattac ataaaatgat gattaattca atactattta ctgatatgtt ccaacacaaa tatttgagag atctagtttt aaactttaat aaaaactatt cctcaaattc aaggagtatc gaagttgaag cacaaaagaa gaagttgtat ttattaattt tactacactc tgtcgatatt tcaaatccac taatgaatac taatatatat ctaaaatggt caagactagt ttatgaagaa ttagaaaacc aaaagaaact aaaactaatg ttaaatattt cactaaatga cacaactgac acgtatgatt atactaaatt gcaaaccagc tttattagct caatctgtat tccttatttt caagcattac taaaatttga cccagaaata gttcaagaat gcattcaaaa cttatacttt aacaatgagt attttgcaaa aactggtcag aaaacaagaa caaataaatg a
Details for CrpaA.19515.c.B2.GE41009
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI LSKEWYNIDL LELSIDEIKE IAEFLIYYRF REVIRDKDDY DDDVLSWQIN KRKIKNFVSE LIKYYHEENP FHNFHHAVSI SITLNNWLDK PSVRHYVNSL EAYCLIISSL SHDLDHPGLT NDMIKELNIL SRKVLQEFTT DEQFFEILQN NTLSTLKIYN NFSVLENHHS SLLFVILNDE ASNIMPPTTS KYYSELHKMM INSILFTDMF QHKYLRDLVL NFNKNYSSNS RSIEVEAQKK KLYLLILLHS VDISNPLMNT NIYLKWSRLV YEELENQKKL KLMLNISLND TTDTYDYTKL QTSFISSICI PYFQALLKFD PEIVQECIQ
Validated NT Sequence
tttgaatgca ttcttgaact atttctgggt caaattttag taatgcttga aaataaggaa tacagattga gctaataaag ctggtttgca atttagtata atcatacgtg tcagttgtgt catttagtga aatatttaac attagtttta gtttcttttg gttttctaat tcttcataaa ctagtcttga ccattttaga tatatattag tattcattag tggatttgaa atatcgacag agtgtagtaa aattaataaa tacaacttct tcttttgtgc ttcaacttcg atactccttg aatttgagga atagttttta ttaaagttta aaactagatc tctcaaatat ttgtgttgga acatatcagt aaatagtatt gaattaatca tcattttatg taattctgaa taatatttgg aagtagttgg aggcataata ttggaagctt catcatttaa tattacaaat aagagagaag aatgatgatt ttccaatact gaaaaattat tatatatttt tagtgttgag agagtattgt tttgtaaaat ttcaaaaaac tgttcatctg ttgtaaattc ttgtaatact tttcttgaga gaatattcaa ctctttaatc atatcattag ttaatcctgg atgatccaaa tcatgtgaaa gagatgaaat gattaaacaa taagcttcta atgaatttac ataatgtctc acagaaggtt tgtctagcca attatttagt gttatggaaa tacttacagc atgatgaaaa ttatgaaaag gattttcttc atggtaatat tttattaact ctgaaacaaa gtttttaatt tttcttttgt ttatttgcca agacaaaaca tcatcatcat aatcgtcttt atcacgtatt acttctctaa atctgtaata gattaaaaat tctgcaattt ctttaatttc atctatactt aattcaagca agtcgatatt ataccattcc ttggaaagta tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHIL SKEWYNIDLL ELSIDEIKEI AEFLIYYRFR EVIRDKDDYD DDVLSWQINK RKIKNFVSEL IKYYHEENPF HNFHHAVSIS ITLNNWLDKP SVRHYVNSLE AYCLIISSLS HDLDHPGLTN DMIKELNILS RKVLQEFTTD EQFFEILQNN TLSTLKIYNN FSVLENHHSS LLFVILNDEA SNIMPPTTSK YYSELHKMMI NSILFTDMFQ HKYLRDLVLN FNKNYSSNSR SIEVEAQKKK LYLLILLHSV DISNPLMNTN IYLKWSRLVY EELENQKKLK LMLNISLNDT TDTYDYTKLQ TSFISSICIP YFQALLKFDP EIVQECIQNL YFNNE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatacttt ccaaggaatg gtataatatc gacttgcttg aattaagtat agatgaaatt aaagaaattg cagaattttt aatctattac agatttagag aagtaatacg tgataaagac gattatgatg atgatgtttt gtcttggcaa ataaacaaaa gaaaaattaa aaactttgtt tcagagttaa taaaatatta ccatgaagaa aatccttttc ataattttca tcatgctgta agtatttcca taacactaaa taattggcta gacaaacctt ctgtgagaca ttatgtaaat tcattagaag cttattgttt aatcatttca tctctttcac atgatttgga tcatccagga ttaactaatg atatgattaa agagttgaat attctctcaa gaaaagtatt acaagaattt acaacagatg aacagttttt tgaaatttta caaaacaata ctctctcaac actaaaaata tataataatt tttcagtatt ggaaaatcat cattcttctc tcttatttgt aatattaaat gatgaagctt ccaatattat gcctccaact acttccaaat attattcaga attacataaa atgatgatta attcaatact atttactgat atgttccaac acaaatattt gagagatcta gttttaaact ttaataaaaa ctattcctca aattcaagga gtatcgaagt tgaagcacaa aagaagaagt tgtatttatt aattttacta cactctgtcg atatttcaaa tccactaatg aatactaata tatatctaaa atggtcaaga ctagtttatg aagaattaga aaaccaaaag aaactaaaac taatgttaaa tatttcacta aatgacacaa ctgacacgta tgattatact aaattgcaaa ccagctttat tagctcaatc tgtattcctt attttcaagc attactaaaa tttgacccag aaatagttca agaatgcatt caaaacttat actttaacaa tgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat