CrpaA.19515.b

Phospodiesterase 5 (PDE5) analog; phosphodiesterasecGMP phosphodiesterase A4

CENTER ID: CrpaA.19515.b
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.19515.b.B2.GE41008 PDE-like domain 632 965
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd3_2320
RefSeq: XP_001388109.1
UniProt: A3FQ29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGLASSPSI GKTILFEKNN DWGLNFDLGE LEIYENQDLK TRLKELISPF ENTFSSVSTL EYLHPFTLKF RSEILEKKFE KKFRKRTEIG IFWIGLLTLF LIILDILLEV YNQKVNEVSN SSVTAYFIIC GVVATCSLFL IISFFVNFLN KHLEVIFTST TIITTILNIT AGNLLLDVSL SPNNNYDPAV QNSRSLIVIT ICSILHFMLP ISFMSILIYS ITSISFYIIL VPSMLMPIPN ETQYVSTTIY LFVFCFVCIL AKRVNVSSSR RDFFNINLVK AEMQILENSM NQTPKSTMIE GTLANINKVQ LFLRYLNTSC TKGINRKMLH DAMVDLTSAQ VTLCRSDDIY AVDPVAIFLP QSLQSNVPQT PRQSKVRIIG SGDVTPKENC SPTASQKIKQ ELGISYDVRI PISPNSPKSM GQFLSPKSHE NKSSYISVDS ISARRNTESR ATVTSIPGPS RRRLSMMIAT AGENSSTLID PSVEKVREYI TAEFTKSSAS RREVLYASKV FDGPAANPSN TNEKIKTTSQ NLDTNHIVES MISRQGPLDE PAVGGDIEDN YRHLLKNKRK IAHFATHLAS KLISIKDPSG TISLLSQSYF NETNPHNFTL PELPKGVGTE LHSDLDRALQ LMNNQYSSNW DLDMWRLNEL TKGNCLSVAG LYMLHLIALE EKNQESGFDS SNLPRGITTA YSSSITAPSK LIGNISKFNI FFRELNIRYL NNKYHNELHG TNVCHLAICL SRATGLWSHL DTVERLASVI ASLGHDVGHI GRTSNFLVNS RHMLAINYND RSVLEMFHAS LTFRIIYYYG GGAADFLQSW DAELHKEFRR IVIELILETD MHRHFECVSR FRVRRQAVDW DPYGDAQDRL MLARTCLKAA DIGHGALKWN QHYKWCRSVV EEFFLQGDEE KALSLPISPI CDRESTDVPK SQVGFLNFVC LPLFQELCYV DVEGDVRRCI DRILENINNW EDIAEAGIQW DSEDALTFVS DEKADLQ
NT Sequence
atgagtgggt tagccagctc tccctctatt gggaagacaa tactatttga gaagaataac gattgggggc ttaattttga tttgggcgag ctcgaaatat atgaaaatca ggacttaaaa actagactaa aggaattaat ttcgccattc gaaaacacat tttcttctgt atctactcta gaatacttgc atccatttac attgaagttc cgaagcgaga ttttggaaaa gaaatttgaa aaaaaattta gaaaaagaac agaaattggt attttttgga ttggattgct aacattattt ttaataatct tggatatctt attagaagtt tacaatcaaa aagtaaatga agtttctaat agttcagtaa ctgcttattt tataatttgt ggtgtagttg caacatgctc attatttcta atcatatcat ttttcgtcaa tttcttaaat aaacatttgg aagttatatt tacatcaact acaataataa caacaatact taatattaca gcaggaaatt tattactaga tgtctcactt tcccctaata ataactacga tcctgctgtc cagaattcca gatcacttat tgtaatcaca atttgctcga ttctgcattt tatgttacca atttctttta tgagtatatt aatttattca ataacgagta tctcattcta tattatattg gtcccttcta tgctgatgcc tattcctaac gaaactcaat acgtatccac aacaatttat ctttttgtgt tctgttttgt atgtattttg gctaaaagag ttaacgtgag ctcttctaga agagactttt ttaacattaa tttagttaaa gcagaaatgc aaatattgga gaattctatg aatcaaacac ctaaatctac aatgatcgaa ggaacattag ctaacatcaa taaggttcaa ctatttcttc gctatttaaa tacttcctgc acaaagggaa taaatcgtaa aatgttacat gacgcaatgg tagacctgac ttctgctcaa gtcactttat gtaggtcaga tgatatttat gcagttgatc cagttgcgat tttcctaccg cagtcacttc agtcaaatgt gccgcaaacc cctagacaat ctaaggttag aataataggt agtggagatg taactccaaa agaaaattgc tctccaactg cttctcaaaa gataaaacaa gagttgggta tatcttatga tgttagaatt cctatatctc ctaattctcc aaagtcaatg gggcagtttt tatctccaaa gtcgcacgag aacaagtcaa gttatataag cgttgattcc atatcagcca gaaggaatac agaatcaagg gctactgtta catcgattcc tggcccttct cgtcgaagac tctcaatgat gattgctact gcaggagaga actcttcaac acttattgat ccgtccgttg aaaaggtgag agagtatatt actgcagaat ttactaaatc gtctgcatct agaagagagg ttttatatgc ttctaaagtt tttgatggac cggccgcaaa cccaagcaat actaatgaaa agatcaagac tacatctcaa aatttggata caaatcatat agtggaaagt atgatttccc gtcaaggacc cttagatgaa cctgcagttg gtggagatat tgaagataat tatagacatc ttttaaagaa taagcgtaag atagctcact tcgcaactca tctagcttca aaactcattt ctattaaaga tccttctgga acaatctcat tattaagtca atcatatttt aatgaaacta atccacataa ttttacgcta cctgaacttc ccaaaggtgt tggtacagaa cttcattctg atttagatag ggcattgcaa cttatgaata accagtattc ttctaattgg gatttggaca tgtggaggct aaacgaatta acaaaaggta attgcctttc agtggcagga ctatatatgc tacacttgat tgcattagaa gaaaaaaacc aggaatctgg ctttgattca tctaacttgc ctaggggaat tactacagca tattcttctt caattactgc tccttcgaag ctaattggca atatatccaa attcaatata ttttttcgag aattaaatat tagatacctc aacaataaat accataacga attacacggc actaacgtat gccatcttgc aatttgccta tcaagagcga ctggattatg gagccattta gacactgtag agaggcttgc ctcagttatt gcatctcttg ggcatgatgt aggccatatt ggtagaacaa gcaattttct tgtaaattca agacatatgc ttgcaattaa ttataatgat cgatctgtcc ttgaaatgtt ccatgcatcc ctaacgtttc ggataatcta ctattatgga ggcggagctg ctgatttttt gcaaagttgg gacgcagaac tccacaaaga atttagaaga atagttattg aattaatatt agagacagat atgcataggc attttgaatg cgtttctagg tttagagtga gaaggcaagc agttgattgg gacccatacg gagatgctca agataggcta atgcttgcta ggacatgttt aaaagctgca gatatcggac atggagcatt gaaatggaat caacactata agtggtgcag atctgttgta gaagaatttt tcctacaagg agatgaggag aaagcattat cgctgcccat ttctccaatt tgtgataggg aatctacaga tgtaccaaag tcgcaggttg gatttttaaa ttttgtttgt cttccattgt tccaagaatt atgctatgta gatgtggagg gtgatgtgag aagatgtatt gatcgaattt tggaaaatat taataactgg gaagacatag ctgaggcagg cattcagtgg gatagtgaag atgcattaac atttgtttca gatgaaaaag ctgatttaca atag
Details for CrpaA.19515.b.B2.GE41008
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MNNQYSSNWD LDMWRLNELT KGNCLSVAGL YMLHLIALEE KNQESGFDSS NLPRGITTAY SSSITAPSKL IGNISKFNIF FRELNIRYLN NKYHNELHGT NVCHLAICLS RATGLWSHLD TVERLASVIA SLGHDVGHIG RTSNFLVNSR HMLAINYNDR SVLEMFHASL TFRIIYYYGG GAADFLQSWD AELHKEFRRI VIELILETDM HRHFECVSRF RVRRQAVDWD PYGDAQDRLM LARTCLKAAD IGHGALKWNQ HYKWCRSVVE EFFLQGDEEK ALSLPISPIC DRESTDVPKS QVGFLNFVCL PLFQELCYVD VEGDV
Validated NT Sequence
tctcacatca ccctccacat ctacatagca taattcttgg aacaatggaa gacaaacaaa atttaaaaat ccaacctgcg actttggtac atctgtagat tccctatcac aaattggaga aatgggcagc gataatgctt tctcctcatc tccttgtagg aaaaattctt ctacaacaga tctgcaccac ttatagtgtt gattccattt caatgctcca tgtccgatat ctgcagcttt taaacatgtc ctagcaagca ttagcctatc ttgagcatct ccgtatgggt cccaatcaac tgcttgcctt ctcactctaa acctagaaac gcattcaaaa tgcctatgca tatctgtctc taatattaat tcaataacta ttcttctaaa ttctttgtgg agttctgcgt cccaactttg caaaaaatca gcagctccgc ctccataata gtagattatc cgaaacgtta gggatgcatg gaacatttca aggacagatc gatcattata attaattgca agcatatgtc ttgaatttac aagaaaattg cttgttctac caatatggcc tacatcatgc ccaagagatg caataactga ggcaagcctc tctacagtgt ctaaatggct ccataatcca gtcgctcttg ataggcaaat tgcaagatgg catacgttag tgccgtgtaa ttcgttatgg tatttattgt tgaggtatct aatatttaat tctcgaaaaa atatattgaa tttggatata ttgccaatta gcttcgaagg agcagtaatt gaagaagaat atgctgtagt aattccccta ggcaagttag atgaatcaaa gccagattcc tggttttttt cttctaatgc aatcaagtgt agcatatata gtcctgccac tgaaaggcaa ttaccttttg ttaattcgtt tagcctccac atgtccaaat cccaattaga agaatactgg ttattcatat ggtggtggtg gtggtgag
Expected Protein Sequence
MAHHHHHHMN NQYSSNWDLD MWRLNELTKG NCLSVAGLYM LHLIALEEKN QESGFDSSNL PRGITTAYSS SITAPSKLIG NISKFNIFFR ELNIRYLNNK YHNELHGTNV CHLAICLSRA TGLWSHLDTV ERLASVIASL GHDVGHIGRT SNFLVNSRHM LAINYNDRSV LEMFHASLTF RIIYYYGGGA ADFLQSWDAE LHKEFRRIVI ELILETDMHR HFECVSRFRV RRQAVDWDPY GDAQDRLMLA RTCLKAADIG HGALKWNQHY KWCRSVVEEF FLQGDEEKAL SLPISPICDR ESTDVPKSQV GFLNFVCLPL FQELCYVDVE GDVRRCIDRI LE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata accagtattc ttctaattgg gatttggaca tgtggaggct aaacgaatta acaaaaggta attgcctttc agtggcagga ctatatatgc tacacttgat tgcattagaa gaaaaaaacc aggaatctgg ctttgattca tctaacttgc ctaggggaat tactacagca tattcttctt caattactgc tccttcgaag ctaattggca atatatccaa attcaatata ttttttcgag aattaaatat tagatacctc aacaataaat accataacga attacacggc actaacgtat gccatcttgc aatttgccta tcaagagcga ctggattatg gagccattta gacactgtag agaggcttgc ctcagttatt gcatctcttg ggcatgatgt aggccatatt ggtagaacaa gcaattttct tgtaaattca agacatatgc ttgcaattaa ttataatgat cgatctgtcc ttgaaatgtt ccatgcatcc ctaacgtttc ggataatcta ctattatgga ggcggagctg ctgatttttt gcaaagttgg gacgcagaac tccacaaaga atttagaaga atagttattg aattaatatt agagacagat atgcataggc attttgaatg cgtttctagg tttagagtga gaaggcaagc agttgattgg gacccatacg gagatgctca agataggcta atgcttgcta ggacatgttt aaaagctgca gatatcggac atggagcatt gaaatggaat caacactata agtggtgcag atctgttgta gaagaatttt tcctacaagg agatgaggag aaagcattat cgctgcccat ttctccaatt tgtgataggg aatctacaga tgtaccaaag tcgcaggttg gatttttaaa ttttgtttgt cttccattgt tccaagaatt atgctatgta gatgtggagg gtgatgtgag aagatgtatt gatcgaattt tggaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat