CrpaA.19515.a

Phospodiesterase 5 (PDE5) analog; phosphodiesterase

CENTER ID: CrpaA.19515.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.19515.a.B2.GE41007 PDE-like domain 1487 1832
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd6_500
RefSeq: XP_627432.1
UniProt: Q5CXM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEHKCNSMNI HSSQDSIKLS TGGSKEKLAD SITGLQNNSS SSSSTNSSFD FLRSKSNSII LSESQSLSSS IIPILKKSDF CDFKKVSLLP LLFGETTCNQ QLTNHNLHDL FDELKQIERY GSNLSYLSNN YNNSVCYRGS SSFGCVQNHS LSNIAEGSSI YCSPEPNNYY NNDVNTLLST TRRRLTPTSI NSSLSSNNII DDNNDEFLCE KKCNAVRRHF EKCLTSKMNG YELCKLLSIY SSVLFQTPLS IVLFYDEETE ELIGSFPSKG TWNSYIPRSL THKWVDYKTT CMHNEHKCPH FKYKTIGEYG QSSKNFINNN GHNHLYSSLT SNSSIQSFQS KAKDFKEARK SETGSSLIRN NSIFQSSNKK YQRLSFIGGY KDKIVNRRAT FPPLSIANRE NEPNTYKNIF DMKSMFNNEM DEIDQRNSVM EYDLLRFSIR NEKILENEKF VKNSDSIDNN NNNFNISING NNIKSSQDNQ NQKKSFLNEI LTQSQKQNFF YFNYSSNICD YIIKSSFIKE DDENKNNVYK EKRSEIFLSN NNQNNDVLFG SYFYNKLKDA EQLPCDIATA TQCSICLENM KLTSLSSSSS SSSSSSSSSS SFSSTSSSSS SFNDSSNGFG RVNNKNNNNN NGNGDGHVYY GNKTNELSMC RNGSEVAYKE EFSSKNDNID IGCINFNNNN TNQQISRKNS GIELIQNKGK KSSEEISDKM SDFPVILVSP PSINDGIINR CCICDKNKNN SNIGNGNNNE KNNEVVLNSV FSDTEIGITN FYQRYSNFKI PVIACPIIDT RIQKDYNNNT NSKVIGIVIC LQPQNPNILL LKSLITEIQA IVCLFLDYKR LLFNTLQLNM NLELYRWVFK PLLSNRRIRH EDNFDYTISI EVISRLCLLI NKITPAQYTL VYVMESCNEF VCIYGPGQFI GLRLCSKNYL ISNILKNKCV NIWNEFKDFS NIFINYSDNN NNNNNNKFTN SQINFKNTAF IPIFNKNDNI NILIQLINRT RDGYCLSNNF DKKKLNHGLS EKKFNNHTME KNNLFDDKSI GINNNSNILS EIEFGKHRLS TGNSNSNGIG TGNGNGIGHN HLSIRRVNTI SSDDYINSLN NSSIDLTKSS NSSFNSVNNG HTKDEERLLI MNNNLLPNLR QRVLASTKAF SFSSSSSSIS SSLLVNRVNT IEYLSPNNDN NVEVKEKESY ETESLVNSNI NNNNNNIIDF SQKDMYIYLI LINQIMQEFH QQLLTLNIHL FYKSLRNPNF NHLYKSNQIM LNSLNNFFGI KFEQRYNNNN NNNNNNNSNN NNNINSIEST FSSSSSSSSS SSPMDNSNMN NHHHEGNKII LDNLLHENNN YSTNLDQFDK NIKDEYNNYK NINKNRYRNL IYKKKSIEKE RCYQSENEYL KQNNQKCEEY FNLNNNYYKE EEEEEEEIQE EPKQEEELEL ELEELEHEEK QQKEEEEEEE EQQQQQQQNY ERLFCRMSSV DEFKKEIKLY NTKEKLIIDS SLSLYREWGF PIWAHGWSVH REFLLNLFKY YGFDSKWNWS NKQLLTLFDL IHDSYNSDIP YHNIFHALQV VQVCYIILRN FGIMLVLNDL NKFILLFAAL CHDIDHPGVN NCFLTISNSK LALKYNDNSI LENHHCNYIF SLLDKCQDLD IRKNFNDFEK KNFRRIFIRS VLSTDMSYHN TLLSNLEEFI STNNNNNNNI NNNNYYYCHN QNLIFDEEEL NNNNINIKML GEIDENSLLI KMDKEFLIET LLHAADISNP ITPFPICAKW SSLIVEEFNY QSKLESQFGL PITPFMDLKD DITKIEAQIG FLEFIIIPQW KLLSRIIPKS IILLKQAEKN RELWLNQNNI IINNNNNNNN NNDNNSCELF KFTLDYLKNN TIGGKDVLEK TSKNKRRITI NNKYNKIINQ IKCDKFLLLK QEKDCNNNSN SNIGNDIDKK DYSKYYIEVE DSVKIEYLKN YGLMKMFTLT LNDMIFD
NT Sequence
atggagcata aatgtaattc tatgaatata catagttctc aagattcaat aaaattatca acaggtggga gtaaagagaa attggcagat tcaataacag gattacaaaa taattcatct tcatcttcat caacaaactc atcatttgat tttttaagaa gtaaatcaaa ttctattatt ttatcagaat ctcaatcatt atcttcaagt ataataccta ttttaaaaaa atcagatttc tgtgatttca aaaaggtatc actattaccg ttattatttg gtgaaacaac ttgcaatcaa cagttaacaa atcataattt acatgattta tttgatgaat taaaacaaat agaaagatat ggaagtaatt tatcatattt aagtaataat tacaataaca gtgtatgtta caggggaagt agtagttttg gatgcgtaca aaatcattct ctttcaaata ttgcagaagg ttcaagtata tattgtagtc cagaacctaa taattattat aataatgatg taaatacgtt attaagtacg acaagaagaa gattaactcc gacatcaata aattcttcat tatcttcaaa taatattata gatgataata atgatgaatt tttatgtgaa aagaaatgta atgcagtaag aagacatttt gaaaaatgtt taacgtcaaa aatgaatggt tatgaattat gtaaattatt aagtatttat tcttcagtat tatttcaaac acctttatca atagtattat tttacgatga agagactgaa gaattaattg gttcatttcc atcaaaagga acatggaact catatatacc aagaagttta acacataaat gggttgatta taaaactaca tgtatgcata atgagcataa atgccctcat tttaaatata aaacaatagg ggaatatgga caatcatcaa agaattttat taacaataat ggtcataatc atttatattc aagtttgaca tcaaattctt caatacaaag ttttcaatct aaggctaaag attttaaaga agcaagaaaa tctgagacag gatcatcttt aattaggaat aatagtattt ttcaatcaag taataaaaaa tatcaaagac taagttttat tggtggatat aaagataaaa tagttaatag aagagcaaca tttcctccat tatcaatagc aaatagagaa aatgaaccaa atacttacaa gaatatattt gatatgaaat caatgtttaa taatgagatg gatgaaatag atcaaagaaa tagtgtaatg gaatatgatt tattaagatt ttcgattaga aatgaaaaga ttcttgaaaa tgagaaattc gtaaagaata gtgacagtat tgataataat aataataact ttaatattag cattaatggt aataatataa aatcttcgca agataatcaa aatcaaaaga aaagtttttt aaatgaaata ttgacacaaa gtcaaaaaca aaatttcttt tattttaatt attcttcaaa tatttgtgat tatataataa aatcaagttt tattaaagaa gatgatgaaa ataaaaataa tgtttataag gaaaagaggt cggaaatttt tttaagtaat aataatcaga ataatgatgt attatttggt tcatattttt ataataaatt aaaagatgct gaacaattac catgtgatat tgcaacagct actcaatgtt caatttgtct tgaaaatatg aaattaacat cattatcttc gtcatcatca tcatcatcat catcttcttc ttcttcttct tcattttctt caacttcatc ttcatcgtca tcatttaatg attcaagtaa tggatttgga agagttaata ataaaaacaa taataataat aatggaaatg gagatggaca tgtttattat ggtaataaaa caaatgaatt atcaatgtgt agaaacggat cagaagtagc atataaagaa gaattttctt caaaaaatga taatattgat attggttgta ttaattttaa taacaataat actaatcaac aaatttcaag aaagaattca ggaattgaat tgatacaaaa taaaggaaag aaatctagtg aagagatttc agataaaatg agtgattttc cagtgatctt agtttcacca ccatctatta atgatggaat aattaataga tgttgtatat gtgataagaa taagaataat agtaatattg gcaatggtaa taataatgaa aaaaataatg aagtggtttt aaattctgta ttttctgata ctgaaatagg aattacaaat ttttatcaga gatatagtaa ttttaagatt ccagttattg cttgtcctat tatagacaca agaattcaaa aagattataa taataataca aattcaaaag tgattggaat agtaatttgt ttacaaccac aaaatccaaa tatattatta ttaaaaagtt taattacaga aattcaagca atcgtttgtt tatttttaga ttataagaga ttattattta atacattaca attaaatatg aatcttgaat tatacagatg ggtatttaaa ccattattat caaatagaag aataagacat gaagacaatt ttgattatac aatatcaatt gaagttattt caagattatg tttattaatc aataaaatta ctcctgcaca atatacatta gtatatgtaa tggaatcttg caatgaattt gtttgcattt atggaccagg acaatttatt ggattacgac tttgttcaaa aaattattta atttcaaata ttcttaaaaa taagtgtgtt aatatatgga atgagtttaa ggatttttca aatattttca taaattattc tgataataat aataataata ataataacaa gtttacaaat tctcaaataa attttaagaa tactgcattt atacccattt ttaataagaa tgataatatt aatatattaa tacaattaat taatagaaca agagatggtt attgtttgag taataatttt gataaaaaga aattaaatca tggattatca gaaaagaaat ttaataatca tacaatggaa aagaataatt tatttgatga taaatcaatt ggtattaata acaattcaaa tatattatca gagattgaat ttggtaaaca cagattatct actggtaata gtaatagtaa tggcattggc actggtaatg gtaatggtat tggccataat catttgtcaa taagaagagt aaatacaata tcttctgatg attatattaa ttcattaaat aattcaagta ttgatttaac aaaatcttca aattcttcat ttaattctgt aaataatggt catacaaaag atgaagaaag attattgatt atgaataata atttattacc aaatttaaga caaagagtat tagcatcgac aaaagctttt tctttttcat catcatcatc atcaatatca tcatctttat tggttaatcg tgttaataca attgaatatt tatctccaaa taatgataat aatgttgaag ttaaagaaaa agagtcatat gaaacagaat ctttggttaa ttcaaatatt aataataata ataataatat tatagatttt tctcaaaaag atatgtatat ttatttaata ttaattaatc aaataatgca agaatttcat caacaattat taacattaaa tattcattta ttttataaat ctttaagaaa tccaaatttt aatcatttat ataaaagtaa tcaaattatg ttaaattcat taaataattt ttttggaatt aaatttgagc aaagatataa caataataat aataataata ataataataa tagtaataac aataataata ttaattcaat agaatccaca ttttcatcat catcatcatc atcttcttct tcttcaccaa tggataattc aaatatgaat aatcatcatc atgaaggaaa taaaataatc ttggataatt tattacatga aaacaataat tattcaacaa atctcgatca atttgataaa aatattaaag atgagtataa taattataaa aatataaata aaaatagata tcgaaattta atatataaaa aaaaatcaat tgaaaaagaa agatgttatc aaagtgaaaa tgagtatcta aaacaaaata atcaaaaatg tgaagaatat ttcaatttaa ataataatta ttataaagaa gaagaagaag aagaagaaga aatacaagaa gaaccaaaac aagaagaaga actagaatta gaattagaag aactagagca tgaagaaaaa caacaaaaag aagaagaaga agaagaagaa gaacaacaac aacaacaaca acaaaattat gagagattat tctgtagaat gagttctgtt gatgaattta aaaaagaaat aaaattatat aatacaaaag aaaaattaat aattgattca tcattaagtt tatatagaga atggggattt ccaatatggg ctcatggatg gtcagtacat agagaatttt tattaaattt attcaaatat tatggatttg atagtaaatg gaattggagt aataaacaat tattaacatt atttgattta atacatgatt cttataattc tgatatacca tatcataata tatttcatgc attacaagtt gtacaagtat gttatataat attaagaaat tttggtataa tgttggtatt aaatgattta aataaattca tattattatt tgcagcttta tgtcatgata ttgatcatcc tggtgtaaat aattgttttt taacaatttc taattcaaaa ttagcattaa agtataatga taattcaatt ttagaaaatc atcattgtaa ttatatattt tcattattag ataaatgtca agatttggat ataagaaaga attttaatga ttttgaaaag aagaatttta gaagaatatt tattagatca gttttatcaa cagatatgag ttatcataat actttattat ctaatttaga agaatttatt tcaactaata ataataataa taataacatt aataataata attattatta ttgtcataat caaaatttaa tttttgatga agaagaatta aataataata atattaatat aaaaatgttg ggagaaattg atgagaattc tttattaata aaaatggata aagaattttt gatagaaaca ttattacatg ctgcagatat atctaatcca attacaccat ttccaatatg tgctaaatgg tcatcattaa tagtagaaga atttaattat caatcaaaat tagaatcaca atttggttta cctattacac cttttatgga tttaaaagat gatataacaa aaatagaagc acaaataggt tttttagaat ttattataat accacaatgg aaattattat caagaattat accaaaaagt attatattac ttaaacaagc tgaaaaaaat agagaattat ggttaaatca gaataatatt attattaata ataataataa taataataac aataatgata ataattcttg tgaattattt aaatttactc tggattattt aaaaaataat acaattggtg gaaaagatgt attagaaaaa acaagcaaaa ataagagaag aattacaatt aacaataaat ataataaaat aattaatcaa attaaatgtg ataaattttt gttattaaaa caagagaaag attgtaataa taatagtaat agtaatattg gaaatgatat tgataaaaaa gattacagca aatattatat agaagttgaa gattcagtaa aaatagagta tttgaagaat tatggtttaa tgaaaatgtt tactttgact ttaaatgata tgatatttga ttag
Details for CrpaA.19515.a.B2.GE41007
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHII DSSLSLYREW GFPIWAHGWS VHREFLLNLF KYYGFDSKWN WSNKQLLTLF DLIHDSYNSD IPYHNIFHAL QVVQVCYIIL RNFGIMLVLN DLNKFILLFA ALCHDIDHPG VNNCFLTISN SKLALKYNDN SILENHHCNY IFSLLDKCQD LDIRKNFNDF EKKNFRRIFI RSVLSTDMSY HNTLLSNLEE FISTNNNNNN NINNNNYYYC HNQNLIFDEE ELNNNNINIK MLGEIDENSL LIKMDKEFLI ETLLHAADIS NPITPFPICA KWSSLIVEEF NYQSKLESQF GLPITPFMDL KDDITKIEAQ IGFLEFIIIP QWKLLSRIIP KSIILLKQAE KNRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catataattg attcatcatt aagtttatat agagaatggg gatttccaat atgggctcat ggatggtcag tacatagaga atttttatta aatttattca aatattatgg atttgatagt aaatggaatt ggagtaataa acaattatta acattatttg atttaataca tgattcttat aattctgata taccatatca taatatattt catgcattac aagttgtaca agtatgttat ataatattaa gaaattttgg tataatgttg gtattaaatg atttaaataa attcatatta ttatttgcag ctttatgtca tgatattgat catcctggtg taaataattg ttttttaaca atttctaatt caaaattagc attaaagtat aatgataatt caattttaga aaatcatcat tgtaattata tattttcatt attagataaa tgtcaagatt tggatataag aaagaatttt aatgattttg aaaagaagaa ttttagaaga atatttatta gatcagtttt atcaacagat atgagttatc ataatacttt attatctaat ttagaagaat ttatttcaac taataataat aataataata acattaataa taataattat tattattgtc ataatcaaaa tttaattttt gatgaagaag aattaaataa taataatatt aatataaaaa tgttgggaga aattgatgag aattctttat taataaaaat ggataaagaa tttttgatag aaacattatt acatgctgca gatatatcta atccaattac accatttcca atatgtgcta aatggtcatc attaatagta gaagaattta attatcaatc aaaattagaa tcacaatttg gtttacctat tacacctttt atggatttaa aagatgatat aacaaaaata gaagcacaaa taggtttttt agaatttatt ataataccac aatggaaatt attatcaaga attataccaa aaagtattat attacttaaa caagctgaaa aaaatagaga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat