SSGCID: Target Page

CrpaA.18981.a

Cyclic nucleotide (cGMP)-dependent protein kinase with 3 cNMP binding domains and a Ser/Thr kinase domain

CENTER ID:	CrpaA.18981.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.18981.a.B1.GE43875	full length		1	965

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd8_750
UniProt:	Q5CWD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAFLTKLKTS KFSSGVKAAK ETTYEELSKE ENDEIEYPEE KMVVFRDRQK ANIQSRIGGL SKNMSNISNA GGGMEDDYEQ NMVISHLKDR EKTEEDIKTI SKALAGNVVG ASLNESEIAT LVSSMHYYEY EVGEVVIEQG ASGFYFFVIS TGSFGVEING NRVNTMSEGT AFGELALIHN TPRSATILVI EKGGLWGLGR STFRDTLRLI SSRNYEENRA FIESLSIFSG LTDKQKSLLS EALVREIFVK DQVIIREKEI GNVLYMIKSG IVGVFVEDKY IRSLNEGDAF GERSLMFDEP RSATVIANAT TECLTLNRGI LTQILGNLGQ VLSKNLIQQS LQNSPIFKQF TKNQMQILLD KISIKTFQKD VILSTAETKA LNMRAFVILE GEVQVSLPSN WLSRRASSLT SMVIDKSIGS VNNYSLSDYI NVNLGRGDYF GDDFVFHPKI PFVCKIEMKS ETKIGVITSS MLSECFGDEN VDVGLEINRK RDAIKSCFVF QYVSEQQLSL LVKSLRLVKF TSGEKIVVQG DKGTAFFILQ SGEVAVYRNN KFIRYLGKND YFGERALLYD ELRSATIEAA TPEVHLWTVD KEAFLKIVEP PMRRYLDERI KLQETRVQMS DLKILQTIGK GTFGVVYMVE NIKSGNKYAL KKISKSRILA LNMQAHVKLE RSILALNDHP FIIKLVRTFK DSENIYLLTE LIPGGELYDA LQRIGLLTRY QAQFYIGSII LALEYLHERS IVYRDLKPEN ILLDSQGYIK LIDFGCAKKI AGRSYTLAGT PHYMAPEVIL GKGYNLSCDA WAIGICLYEF LCGCLPFGND AVDHLEIFKD ILTSKLVFPR HLNDVDTVNI IKRLLCRVPE VRMGCSATGY KEIKDNVFFK DFNFDRLLGR SYVAPLVRKY RVFAKNTENE NIVENITEVS QNEEALSPNT HNIKSKQKIS FNASKNHSVS TRGNKMDEYD WDIEF

NT Sequence

atggcattcc taacaaagtt aaaaacgagt aaattttctt ctggtgtaaa agcggccaaa gaaacaacat atgaagagtt atcaaaggag gaaaatgatg aaattgaata tccagaagag aagatggtgg tctttagaga tagacaaaaa gcaaatattc agtcaagaat tggaggctta agcaagaata tgagtaatat aagtaatgct ggtggcggaa tggaggatga ttatgaacag aatatggtaa tatctcactt aaaggataga gaaaagacag aagaggacat aaaaactatt tccaaagcat tggcaggaaa cgtagtggga gcttcactga atgaatctga aattgctact ttggtcagtt ccatgcatta ttacgaatat gaagtggggg aagtggtaat tgagcaagga gcatctggat tttacttttt tgtcatttca acaggaagtt ttggggttga gattaatggt aacagagtca acacaatgtc agaaggaaca gcttttggag aattagcgct tattcacaat actccaagaa gtgctaccat tcttgttatt gaaaaaggag gactttgggg acttggaaga tccactttta gagatacact gagacttatt tcatctcgaa attatgagga aaatagggca ttcattgaaa gcttatcaat attttcaggt cttactgaca aacaaaagag tctcttatct gaagctttag ttagagagat ttttgttaaa gatcaggtca ttattagaga aaaagaaatt ggtaatgtat tatacatgat taagagtggt attgttggag tttttgttga agataaatat attcgttctt taaatgaggg agacgccttt ggagagagat ctctaatgtt tgacgaacca aggtctgcaa cagtaatagc aaatgcaaca actgaatgtt taactttgaa tagaggaatt cttacccaga ttttaggtaa cttaggacaa gttctgagca aaaacttaat tcaacaatct ctacaaaatt ctccaatatt taagcaattt acaaaaaatc aaatgcaaat attacttgat aagatttcta ttaagacttt tcaaaaagac gttattttgt ctactgcaga gactaaagca cttaatatga gagcctttgt catcttggaa ggagaggtgc aagtctctct tccatcaaat tggctttcta gaagggccag tagtttaact tctatggtta tcgataaatc tattggttct gtcaataatt actcactttc tgattacatt aatgttaact taggaagagg agattatttt ggggatgact ttgtttttca tccaaaaatt ccatttgtat gcaagattga aatgaaaagt gaaactaaaa ttggagtaat cacatcttca atgctttctg aatgctttgg ggatgagaat gttgatgttg gacttgaaat taacagaaaa agagatgcaa tcaagtcgtg ctttgtcttc caatacgttt ctgagcaaca actaagtctt ttggtaaagt ctttaaggct tgtaaagttt actagcggtg aaaaaattgt tgtacaaggt gacaaaggaa ctgctttttt cattttacag tctggagaag tagctgttta tagaaataac aagtttattc gttatcttgg aaaaaatgac tactttggcg aaagagctct gctttatgat gaattaagat ctgctactat tgaggctgca acccctgagg tgcatctatg gactgtagac aaagaagcct ttcttaaaat tgttgaacct ccaatgagac gatatcttga tgaaagaata aagctacagg aaaccagagt gcaaatgagt gatttaaaga ttcttcaaac aattggtaaa ggaacatttg gagtcgttta tatggttgaa aatattaaat caggaaataa atatgcactt aagaaaattt ccaagtcacg aattttggcg ctcaatatgc aggcacatgt aaaattggaa aggtccattt tggcgctcaa tgaccatcca tttattatta aacttgttag aacatttaag gattcagaaa atatatattt gttgacagaa ttgattcctg gaggagagct atatgatgca ttacaaagaa ttggtttatt aacacggtat caagctcaat tttatattgg ctcaattatt ctagctctcg agtatttaca tgagagatct attgtatata gagatttgaa gcctgaaaat atattactag attctcaggg atatattaaa ttgattgatt ttggttgtgc aaaaaagatt gctggtagaa gttatacttt ggcaggtacc ccacattaca tggctccaga ggttattctt ggaaagggat ataacttaag ttgtgatgca tgggctattg gaatttgctt atatgagttt ttatgtggtt gtcttccatt tggaaacgat gctgttgacc atttggagat ttttaaggat attttgactt caaagttggt atttccaaga catttgaatg atgttgatac tgttaatata ataaagagat tattatgtag ggttccagag gttaggatgg gatgttcagc tactggctat aaggaaatca aggacaatgt attttttaag gattttaatt ttgataggtt gttaggcagg agttatgttg ctccattagt taggaagtat cgagtttttg caaaaaatac agaaaatgag aatatagttg agaatattac tgaagttagt cagaatgagg aggctttgag tcctaatacc cataatatta agtccaagca gaaaataagt ttcaatgctt ccaaaaatca ctctgtaagt acaagaggaa acaagatgga tgagtatgat tgggatatag agttttaa

Details for CrpaA.18981.a.B1.GE43875

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/2/2020
EXPECTED MW:	110kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	28

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatggca ttcctaacaa agttaaaaac gagtaaattt tcttctggtg taaaagcggc caaagaaaca acatatgaag agttatcaaa ggaggaaaat gatgaaattg aatatccaga agagaagatg gtggtcttta gagatagaca aaaagcaaat attcagtcaa gaattggagg cttaagcaag aatatgagta atataagtaa tgctggtggc ggaatggagg atgattatga acagaatatg gtaatatctc acttaaagga tagagaaaag acagaagagg acataaaaac tatttccaaa gcattggcag gaaacgtagt gggagcttca ctgaatgaat ctgaaattgc tactttggtc agttccatgc attattacga atatgaagtg ggggaagtgg taattgagca aggagcatct ggattttact tttttgtcat ttcaacagga agttttgggg ttgagattaa tggtaacaga gtcaacacaa tgtcagaagg aacagctttt ggagaattag cgcttattca caatactcca agaagtgcta ccattcttgt tattgaaaaa ggaggacttt ggggacttgg aagatccact tttagagata cactgagact tatttcatct cgaaattatg aggaaaatag ggcattcatt gaaagcttat caatattttc aggtcttact gacaaacaaa agagtctctt atctgaagct ttagttagag agatttttgt taaagatcag gtcattatta gagaaaaaga aattggtaat gtattataca tgatta

Expected Protein Sequence

MAHHHHHHMA FLTKLKTSKF SSGVKAAKET TYEELSKEEN DEIEYPEEKM VVFRDRQKAN IQSRIGGLSK NMSNISNAGG GMEDDYEQNM VISHLKDREK TEEDIKTISK ALAGNVVGAS LNESEIATLV SSMHYYEYEV GEVVIEQGAS GFYFFVISTG SFGVEINGNR VNTMSEGTAF GELALIHNTP RSATILVIEK GGLWGLGRST FRDTLRLISS RNYEENRAFI ESLSIFSGLT DKQKSLLSEA LVREIFVKDQ VIIREKEIGN VLYMIKSGIV GVFVEDKYIR SLNEGDAFGE RSLMFDEPRS ATVIANATTE CLTLNRGILT QILGNLGQVL SKNLIQQSLQ NSPIFKQFTK NQMQILLDKI SIKTFQKDVI LSTAETKALN MRAFVILEGE VQVSLPSNWL SRRASSLTSM VIDKSIGSVN NYSLSDYINV NLGRGDYFGD DFVFHPKIPF VCKIEMKSET KIGVITSSML SECFGDENVD VGLEINRKRD AIKSCFVFQY VSEQQLSLLV KSLRLVKFTS GEKIVVQGDK GTAFFILQSG EVAVYRNNKF IRYLGKNDYF GERALLYDEL RSATIEAATP EVHLWTVDKE AFLKIVEPPM RRYLDERIKL QETRVQMSDL KILQTIGKGT FGVVYMVENI KSGNKYALKK ISKSRILALN MQAHVKLERS ILALNDHPFI IKLVRTFKDS ENIYLLTELI PGGELYDALQ RIGLLTRYQA QFYIGSIILA LEYLHERSIV YRDLKPENIL LDSQGYIKLI DFGCAKKIAG RSYTLAGTPH YMAPEVILGK GYNLSCDAWA IGICLYEFLC GCLPFGNDAV DHLEIFKDIL TSKLVFPRHL NDVDTVNIIK RLLCRVPEVR MGCSATGYKE IKDNVFFKDF NFDRLLGRSY VAPLVRKYRV FAKNTENENI VENITEVSQN EEALSPNTHN IKSKQKISFN ASKNHSVSTR GNKMDEYDWD IEF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat tcctaacaaa gttaaaaacg agtaaatttt cttctggtgt aaaagcggcc aaagaaacaa catatgaaga gttatcaaag gaggaaaatg atgaaattga atatccagaa gagaagatgg tggtctttag agatagacaa aaagcaaata ttcagtcaag aattggaggc ttaagcaaga atatgagtaa tataagtaat gctggtggcg gaatggagga tgattatgaa cagaatatgg taatatctca cttaaaggat agagaaaaga cagaagagga cataaaaact atttccaaag cattggcagg aaacgtagtg ggagcttcac tgaatgaatc tgaaattgct actttggtca gttccatgca ttattacgaa tatgaagtgg gggaagtggt aattgagcaa ggagcatctg gattttactt ttttgtcatt tcaacaggaa gttttggggt tgagattaat ggtaacagag tcaacacaat gtcagaagga acagcttttg gagaattagc gcttattcac aatactccaa gaagtgctac cattcttgtt attgaaaaag gaggactttg gggacttgga agatccactt ttagagatac actgagactt atttcatctc gaaattatga ggaaaatagg gcattcattg aaagcttatc aatattttca ggtcttactg acaaacaaaa gagtctctta tctgaagctt tagttagaga gatttttgtt aaagatcagg tcattattag agaaaaagaa attggtaatg tattatacat gattaagagt ggtattgttg gagtttttgt tgaagataaa tatattcgtt ctttaaatga gggagacgcc tttggagaga gatctctaat gtttgacgaa ccaaggtctg caacagtaat agcaaatgca acaactgaat gtttaacttt gaatagagga attcttaccc agattttagg taacttagga caagttctga gcaaaaactt aattcaacaa tctctacaaa attctccaat atttaagcaa tttacaaaaa atcaaatgca aatattactt gataagattt ctattaagac ttttcaaaaa gacgttattt tgtctactgc agagactaaa gcacttaata tgagagcctt tgtcatcttg gaaggagagg tgcaagtctc tcttccatca aattggcttt ctagaagggc cagtagttta acttctatgg ttatcgataa atctattggt tctgtcaata attactcact ttctgattac attaatgtta acttaggaag aggagattat tttggggatg actttgtttt tcatccaaaa attccatttg tatgcaagat tgaaatgaaa agtgaaacta aaattggagt aatcacatct tcaatgcttt ctgaatgctt tggggatgag aatgttgatg ttggacttga aattaacaga aaaagagatg caatcaagtc gtgctttgtc ttccaatacg tttctgagca acaactaagt cttttggtaa agtctttaag gcttgtaaag tttactagcg gtgaaaaaat tgttgtacaa ggtgacaaag gaactgcttt tttcatttta cagtctggag aagtagctgt ttatagaaat aacaagttta ttcgttatct tggaaaaaat gactactttg gcgaaagagc tctgctttat gatgaattaa gatctgctac tattgaggct gcaacccctg aggtgcatct atggactgta gacaaagaag cctttcttaa aattgttgaa cctccaatga gacgatatct tgatgaaaga ataaagctac aggaaaccag agtgcaaatg agtgatttaa agattcttca aacaattggt aaaggaacat ttggagtcgt ttatatggtt gaaaatatta aatcaggaaa taaatatgca cttaagaaaa tttccaagtc acgaattttg gcgctcaata tgcaggcaca tgtaaaattg gaaaggtcca ttttggcgct caatgaccat ccatttatta ttaaacttgt tagaacattt aaggattcag aaaatatata tttgttgaca gaattgattc ctggaggaga gctatatgat gcattacaaa gaattggttt attaacacgg tatcaagctc aattttatat tggctcaatt attctagctc tcgagtattt acatgagaga tctattgtat atagagattt gaagcctgaa aatatattac tagattctca gggatatatt aaattgattg attttggttg tgcaaaaaag attgctggta gaagttatac tttggcaggt accccacatt acatggctcc agaggttatt cttggaaagg gatataactt aagttgtgat gcatgggcta ttggaatttg cttatatgag tttttatgtg gttgtcttcc atttggaaac gatgctgttg accatttgga gatttttaag gatattttga cttcaaagtt ggtatttcca agacatttga atgatgttga tactgttaat ataataaaga gattattatg tagggttcca gaggttagga tgggatgttc agctactggc tataaggaaa tcaaggacaa tgtatttttt aaggatttta attttgatag gttgttaggc aggagttatg ttgctccatt agttaggaag tatcgagttt ttgcaaaaaa tacagaaaat gagaatatag ttgagaatat tactgaagtt agtcagaatg aggaggcttt gagtcctaat acccataata ttaagtccaa gcagaaaata agtttcaatg cttccaaaaa tcactctgta agtacaagag gaaacaagat ggatgagtat gattgggata tagagttttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat