CrpaA.18896.a
Threonyl-tRNA synthetase (RNA binding domain TGS+HxxxH+tRNA synthetase)
CENTER ID:
CrpaA.18896.a
ORGANISM:
Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:
cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
CrpaA.18896.a.B3.GE43701
TEdwards Stma structure domain
info
330
763
CrpaA.18896.a.B3.GE43701
CrpaA.18896.a.MB1.GE39283
full length
info
1
763
CrpaA.18896.a.MB1.GE39283
CrpaA.18896.a.MB2.GE39284
potential soluble domain
info
333
736
CrpaA.18896.a.MB2.GE39284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
CrpaA.18896.a.MB1.PW37843
full length
info
1
763
PW37843
Structures
7L3O
DEPOSITED: 12/18/2020
DETERMINATION: XRay
CLONE: CrpaA.18896.a.B3.GE43701
PROTEIN: CrpaA.18896.a.B3.PW38874
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPKRIQKGT EKTLSSVGKI GGEFKVEKKP EFINRRLSLF KTLYEKQCAL LQEKASKNEP IKITLQNGDQ RDGVKFQTSP MEIARQISKK LAESCIVAKI RYNKSLFIED DEVRGIEEDE EETCCQGHEN QSDGWRLWDA TRPLEGDCEL EILTFDSLEG SGVFWHSSSH ILGQCLENEY GAQVTIGPAL NPGFYYDSYM GTHSVSNTEY SDIENCAKTI ISEKQQFERL QCNKEEALEL FKDNPFKVSL IMSKIPDGAQ TTIYRCGSFV DLCTGPHIPH TGIVKAFKVT KNSGCNWLGN TENDALQRVY GVSFPDKKRL DEYLNMLEEA KKRDHRLLGS NLQLFFFDSN VSPGSCFWLP AGARLYNKLM DFIRNEYRIR EFTEVITPNI FSCDLWKTSG HYFAYKENMF IFDVEEKEWG LKPMNCPGHC VMFKHMNPSY RQLPIRLADF GVLHRNEFSG ALNGLTRVRR FQQDDAHIFC TPEQIQEEVF KALDFLFFIY GQLGFTFDLF LSTMPKEHLG TEEQWKEAEN ALKSALDKTG RDWKLNPGDG AFYGPKIDIM LWDALKRQHQ CGTIQLDFQL PIRFNLQYRT DENISEESTN QEQPPSHQNA DQNPDNSLKQ GYRRPVIIHR AILGSVERMS AVILEHTGGK LPFWLSPRQA IVLSISEKTV EYAKSVEREL CRRGFDVSGD YSAATINKKI RESQLLQWNY MLVIGENEAR DKKVTLRCRD TTIPQELLTL DQLILKFSSM GFPSSIDSNS SNQ
NT Sequence
atggatccaa agagaattca aaaaggtact gaaaaaacac tttccagtgt tggaaaaatt ggtggagaat tcaaagtaga aaagaagcca gaattcataa atagacgtct ctcactattc aaaacgcttt atgaaaaaca atgtgcattg ctccaggaaa aggcttcaaa aaatgaacca attaaaatta cccttcagaa tggtgatcaa agagacggtg ttaagttcca aacatcccct atggaaattg caagacaaat atcaaagaaa ttagcggaga gctgcattgt agcaaaaatc agatataaca aatcattatt tattgaggat gatgaagttc gtggtattga agaagatgaa gaagaaacat gttgtcaagg gcacgaaaat caaagtgatg gatggagact ttgggatgca actagaccat tagaagggga ttgtgaatta gagattttaa catttgattc tcttgaaggt agtggtgttt tctggcactc ttcatcacat attcttggtc aatgtttaga aaatgaatat ggagctcaag ttacaattgg tcctgcactt aatccaggat tttactatga ttcatacatg ggaactcaca gtgttagtaa tactgaatat tctgatattg aaaattgtgc aaaaacaata atttctgaaa agcaacaatt tgaaaggtta caatgcaata aagaagaagc attggaactc tttaaagata acccttttaa ggtttcattg atcatgtcaa agatcccaga tggtgctcaa acaacaatat atagatgtgg ttcattcgta gatttatgta caggtcccca tattcctcat acagggattg ttaaggcttt taaagttaca aagaattctg gatgtaactg gcttggaaat actgaaaatg atgcattaca aagagtttat ggtgtatctt tcccagataa gaaacgctta gatgagtatt tgaatatgtt ggaagaggct aaaaagcgtg atcatagatt acttggatca aacttacagc tattcttctt cgactcaaat gtatcgcctg gatcatgttt ctggcttcca gcaggagcaa gactgtataa taagttaatg gactttatta gaaatgaata cagaattcgt gaatttactg aagttattac cccaaacatt tttagttgcg atttgtggaa gacttcagga cattactttg cttacaaaga aaatatgttt atttttgatg tagaagaaaa ggaatggggt ctaaaaccaa tgaactgtcc aggacactgt gttatgttca aacatatgaa tccatcatat agacaacttc caattagatt agctgatttt ggtgtacttc acagaaatga attttctggt gctttaaatg gcttaactag agttagaaga ttccaacaag atgatgcgca tatcttctgt actcctgaac agatccaaga agaagtattt aaagctcttg actttttatt cttcatatat ggtcaattgg gattcacatt cgacctcttt ctaagtacaa tgccaaaaga acatttggga acagaagaac agtggaaaga ggcagaaaat gcattaaagt ctgcattgga taagactggt agagactgga aattaaaccc tggggatgga gcattttatg gcccaaaaat tgatattatg ctttgggatg ccttaaaaag gcaacatcaa tgcggaacaa tccaacttga cttccagctc ccaattagat ttaatctcca atatagaact gacgaaaata tttctgaaga gtccacaaat caagaacaac ctccctctca ccaaaatgct gatcaaaacc cagacaatag tcttaaacaa ggatacagaa ggccagtaat tattcacaga gcaattcttg gtagtgtaga aagaatgtca gctgtaattc ttgaacatac aggaggaaaa ttaccctttt ggctttctcc aaggcaagcc attgttctct ctatctcaga aaaaactgtt gaatatgcta aatctgttga aagagagctt tgcagaagag gttttgacgt ttctggagat tacagtgctg ccaccattaa caaaaaaatt agagaatcac aacttttgca atggaactac atgcttgtta ttggcgaaaa cgaagcaaga gacaaaaaag tcactctccg ctgcagagat accactattc cccaagaatt acttactttg gatcaactta ttctcaagtt ctcttctatg ggcttccctt caagtatcga ctcaaattct tctaaccaat ga
Details for CrpaA.18896.a.B3.GE43701
HARVESTED ON:
7/6/2020
SEQUENCED ON:
6/23/2020
EXPECTED MW:
51kDa
OBSERVED MW:
51kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
99
PERCENT COVERAGE:
98
Details for CrpaA.18896.a.MB1.GE39283
HARVESTED ON:
6/26/2015
SEQUENCED ON:
7/24/2015
EXPECTED MW:
133kDa
OBSERVED MW:
170kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18896.a.MB2.GE39284
HARVESTED ON:
6/25/2015
SEQUENCED ON:
7/24/2015
EXPECTED MW:
92kDa
OBSERVED MW:
99kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Insoluble
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
44
Details for CrpaA.18896.a.MB1.PW37843
PURIFICATION DATe:
2/1/2016
CONCENTRATION:
20mg/ml
OBSERVED MW:
130kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
2
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable