CrpaA.18681.a
Aminoacyl-tRNA synthetase, probable; proline-tRNA synthetase
| CENTER ID: | CrpaA.18681.a |
| ORGANISM: | Cryptosporidium parvum Iowa II |
| ASSOCIATED DISEASE: | cryptosporidiosis - (diarrheal disease) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.18681.a.B1.GE38636 | full length | 1 | 688 | ||
| CrpaA.18681.a.B3.GE39089 | soluble domain #2 | 186 | 688 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.18681.a.B1.PW37640 | full length | 1 | 688 |
Structures
5F9Y
DEPOSITED: 12/10/2015
DETERMINATION: XRay
CLONE: CrpaA.18681.a.B3.GE39089
PROTEIN: CrpaA.18681.a.B3.PW37711
5F9Z
DEPOSITED: 12/10/2015
DETERMINATION: XRay
CLONE: CrpaA.18681.a.B3.GE39089
PROTEIN: CrpaA.18681.a.B3.PW37711
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | CryptoDB:cgd6_4400 |
| UniProt: | Q7YZ69 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVDEKLYSE VLDQLSKLSI NYSKFDHDAT PNMESMVEVL KEQAEKHNTD FAKNLLIKSK GNEGLFFVLA HHETDTKMKN LGQIFGVSGN KLRLADEDVL NDTLKVKRGC LTPLSLIFEK SGGIQVYFDE CLKDKKVFVH PLTNTESFSI HINDIVKFAE SCGKKVKWFS MDNLEEQKPA GKPEESKENE SLLGITADKI TSFADWYSQV IVKSEMIEYY DISGCYILRP WSYFIWETIQ SVFDQKIKQH DVQNAYFPIF VTQKKLETEK DHVEGFSPEV AWVTKSGKSD LAEPIAIRPT SETIMYPYFA KWIRSHRDLP LKINQWTSIV RWEFKHPTPF IRTREFLWQE GHTAHSTRKE ALEMVDIILN EYASIYEDLL ATPVVKGTKS ENEKFPGGDI TKSIEGFIPE IGRAVQAATS HLLGQNFSKM FGVEFEDEKG NKEYAHQTSW GLTTRAIGVM IMTHGDNKGL VLPPKVAPVQ VIIIPIIFKT VITEEQKKIC NEVECILKKA GVRVKIDDRS NYTPGWKYNH WEVKGVCLRF EVGPRDIEKR SVRVVVRDNM EKMDIPISEL ESKIPKLLEE FQNRLLFKAK QRQNESIIRV DTFDKVMDTL NQKKMVIAPW CEDVSCEEEI KKETARLSLD NEDNQSMTGA MKSLCIPNDQ IFKIEEGKTK CFFCDKLAKK FTLFGRSY
NT Sequence
atgtcagtgg atgaaaaact atactccgag gtattagatc aattgagcaa gctctctata aactattcta agtttgatca tgatgctacc ccaaatatgg aatctatggt ggaagtgctt aaagagcagg cagaaaaaca caatactgac tttgcaaaaa atctattaat aaaatcaaaa ggtaacgaag gcttattttt tgttttggca caccacgaaa ccgatacgaa aatgaagaac cttgggcaga tctttggtgt gagtggaaac aagcttagat tagctgatga agatgtttta aatgatactc ttaaggtaaa aaggggatgc ttaactcctt tatctttaat atttgagaaa tctggaggaa ttcaagttta ctttgatgaa tgtcttaaag acaagaaagt atttgtacat ccactaacca atactgaaag cttttcaatc catattaatg acattgtaaa atttgctgaa tcatgtggga agaaagttaa atggttttct atggataatt tagaggaaca gaaacctgca ggaaagcctg aagaatctaa ggaaaacgaa agtttgcttg gaattacagc agacaaaata acttcttttg cagactggta ttcccaagtt atagtaaagt ctgaaatgat tgaatactat gatatctctg ggtgttacat attacgccca tggagttact ttatttggga aacaattcaa agcgttttcg atcagaaaat caaacaacat gatgtacaaa atgcctattt ccccattttt gtaacccaaa aaaagttaga aactgaaaaa gatcacgttg aaggattctc tcctgaagtt gcatgggtaa ctaaatcagg aaaatcagat ttagccgagc caatagctat ccgtccaaca tctgaaacaa tcatgtatcc atattttgcc aaatggatta gatcccatag ggatttgcca cttaagatta atcaatggac ttccattgta agatgggaat ttaagcatcc aactccattc atcagaacac gtgaattttt atggcaagaa ggacatacag cccattctac tagaaaagag gctttagaaa tggttgacat cattttaaat gaatatgcat ctatttatga agatttattg gcaactcctg tagttaaagg taccaaatct gagaatgaaa aattccctgg gggtgatata actaaatcaa ttgaaggatt cattccagaa attggtcgcg ctgttcaggc tgcaacttct catttacttg gacaaaactt tagcaaaatg tttggtgttg aatttgaaga tgaaaaagga aacaaagaat atgcacatca aacttcttgg ggcttaacta caagagctat tggtgttatg attatgacac atggagataa taaaggttta gtattgccac ctaaggttgc acctgtacaa gttattatta ttcccatcat ttttaaaacc gttatcactg aggaacagaa gaaaatctgc aatgaagtgg agtgtatttt gaagaaggct ggggttagag taaagatcga tgatagatct aactatactc ctggctggaa atataaccat tgggaagtta aaggtgtatg cttgagattt gaagttggac cgcgtgatat cgaaaagaga agtgttcgtg ttgttgttag agataatatg gagaaaatgg atattccaat ttccgaattg gaatcaaaga taccaaagtt gttggaagaa ttccaaaata gactgttatt taaggccaaa caacgacaaa atgaatctat cataagagtt gatacatttg acaaagttat ggatacactg aaccaaaaga agatggttat agctccttgg tgtgaggatg tatcatgcga ggaagaaatt aaaaaggaaa ctgcaagact tagtttggat aatgaagaca atcagtccat gaccggtgca atgaaatcct tgtgtatacc taatgatcaa atatttaaaa tagaagaagg aaaaacaaag tgtttcttct gtgataaact cgcaaagaaa tttacactat ttggaagaag ttactaa
Details for CrpaA.18681.a.B1.GE38636
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 80kDa |
| OBSERVED MW: | 80kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS VDEKLYSEVL DQLSKLSINY SKFDHDATPN MESMVEVLKE QAEKHNTDFA KNLLIKSKGN EGLFFVLAHH ETDTKMKNLG QIFGVSGNKL RLADEDVLND TLKVKRGCLT PLSLIFEKSG GIQVYFDECL KDKKVFVHPL TNTESFSIHI NDIVKFAESC GKKVKWFSMD NLEEQKPAGK PEESKENESL LGITADKITS FADWYSQVIV KSEMIEYYDI SGCYILRPWS YFIWETIQSV FDQKIKQHDV QNAYFPIFVT QKKLETEKDH VEGFSPEVAW VTKSGKSDLA EPIAIRPTSE TIMYPYFAKW IRSHRDLPLK INQWTSIVRW EFKHPTPFIR TREFLWQEGH TAHSTRKEAL EMVDIILNEY ASIYEDLLAT PVVKGTKSEN EKFPGGDITK SIEGFIPEIG RAVQAATSHL LGQNFSKMFG VEFEDEKGNK EYAHQTSWGL TTRAIGVMIM THGDNKGLVL PPKVAPVQVI IIPIIFKTVI TEEQKKICNE VECILKKAGV RVKIDDRSNY TPGWKYNHWE VKGVCLRFEV GPRDIEKRSV RVVVRDNMEK MDIPISELES KIPKLLEEFQ NRLLFKAKQR QNESIIRVDT FDKVMDTLNQ KKMVIAPWCE DVSCEEEIKK ETARLSLDNE DNQSMTGAMK SLCIPNDQIF KIEEGKTKCF FCDKLAKKFT LFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag tggatgaaaa actatactcc gaggtattag atcaattgag caagctctct ataaactatt ctaagtttga tcatgatgct accccaaata tggaatctat ggtggaagtg cttaaagagc aggcagaaaa acacaatact gactttgcaa aaaatctatt aataaaatca aaaggtaacg aaggcttatt ttttgttttg gcacaccacg aaaccgatac gaaaatgaag aaccttgggc agatctttgg tgtgagtgga aacaagctta gattagctga tgaagatgtt ttaaatgata ctcttaaggt aaaaagggga tgcttaactc ctttatcttt aatatttgag aaatctggag gaattcaagt ttactttgat gaatgtctta aagacaagaa agtatttgta catccactaa ccaatactga aagcttttca atccatatta atgacattgt aaaatttgct gaatcatgtg ggaagaaagt taaatggttt tctatggata atttagagga acagaaacct gcaggaaagc ctgaagaatc taaggaaaac gaaagtttgc ttggaattac agcagacaaa ataacttctt ttgcagactg gtattcccaa gttatagtaa agtctgaaat gattgaatac tatgatatct ctgggtgtta catattacgc ccatggagtt actttatttg ggaaacaatt caaagcgttt tcgatcagaa aatcaaacaa catgatgtac aaaatgccta tttccccatt tttgtaaccc aaaaaaagtt agaaactgaa aaagatcacg ttgaaggatt ctctcctgaa gttgcatggg taactaaatc aggaaaatca gatttagccg agccaatagc tatccgtcca acatctgaaa caatcatgta tccatatttt gccaaatgga ttagatccca tagggatttg ccacttaaga ttaatcaatg gacttccatt gtaagatggg aatttaagca tccaactcca ttcatcagaa cacgtgaatt tttatggcaa gaaggacata cagcccattc tactagaaaa gaggctttag aaatggttga catcatttta aatgaatatg catctattta tgaagattta ttggcaactc ctgtagttaa aggtaccaaa tctgagaatg aaaaattccc tgggggtgat ataactaaat caattgaagg attcattcca gaaattggtc gcgctgttca ggctgcaact tctcatttac ttggacaaaa ctttagcaaa atgtttggtg ttgaatttga agatgaaaaa ggaaacaaag aatatgcaca tcaaacttct tggggcttaa ctacaagagc tattggtgtt atgattatga cacatggaga taataaaggt ttagtattgc cacctaaggt tgcacctgta caagttatta ttattcccat catttttaaa accgttatca ctgaggaaca gaagaaaatc tgcaatgaag tggagtgtat tttgaagaag gctggggtta gagtaaagat cgatgataga tctaactata ctcctggctg gaaatataac cattgggaag ttaaaggtgt atgcttgaga tttgaagttg gaccgcgtga tatcgaaaag agaagtgttc gtgttgttgt tagagataat atggagaaaa tggatattcc aatttccgaa ttggaatcaa agataccaaa gttgttggaa gaattccaaa atagactgtt atttaaggcc aaacaacgac aaaatgaatc tatcataaga gttgatacat ttgacaaagt tatggataca ctgaaccaaa agaagatggt tatagctcct tggtgtgagg atgtatcatg cgaggaagaa attaaaaagg aaactgcaag acttagtttg gataatgaag acaatcagtc catgaccggt gcaatgaaat ccttgtgtat acctaatgat caaatattta aaatagaaga aggaaaaaca aagtgtttct tctgtgataa actcgcaaag aaatttacac tatttggaag aagttactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for CrpaA.18681.a.B3.GE39089
| HARVESTED ON: | 3/31/2015 |
| SEQUENCED ON: | 3/27/2015 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS KENESLLGIT ADKITSFADW YSQVIVKSEM IEYYDISGCY ILRPWSYFIW ETIQSVFDQK IKQHDVQNAY FPIFVTQKKL ETEKDHVEGF SPEVAWVTKS GKSDLAEPIA IRPTSETIMY PYFAKWIRSH RDLPLKINQW TSIVRWEFKH PTPFIRTREF LWQEGHTAHS TRKEALEMVD IILNEYASIY EDLLATPVVK GTKSENEKFP GGDITKSIEG FIPEIGRAVQ AATSHLLGQN FSKMFGVEFE DEKGNKEYAH QTSWGLTTRA IGVMIMTHGD NKGLVLPPKV APVQVIIIPI IFKTVITEEQ KKICNEVECI LKKAGVRVKI DDRSNYTPGW KYNHWEVKGV CLRFEVGPRD IEKRSVRVVV RDNMEKMDIP ISELESKIPK LLEEFQNRLL FKAKQRQNES IIRVDTFDKV MDTLNQKKMV IAPWCEDVSC EEEIKKETAR LSLDNEDNQS MTGAMKSLCI PNDQIFKIEE GKTKCFFCDK LAKKFTLFGR SY
Validated NT Sequence
atcttactca cttagtaact tcttccaaat agtgtaaatt tctttgcgag tttatcacag aagaaacact ttgtttttcc ttcttctatt ttaaatattt gatcattagg tatacacaag gatttcattg caccggtcat ggactgattg tcttcattat ccaaactaag tcttgcagtt tcctttttaa tttcttcctc gcatgataca tcctcacacc aaggagctat aaccatcttc ttttggttca gtgtatccat aactttgtca aatgtatcaa ctcttatgat agattcattt tgtcgttgtt tggccttaaa taacagtcta ttttggaatt cttccaacaa ctttggtatc tttgattcca attcggaaat tggaatatcc attttctcca tattatctct aacaacaaca cgaacacttc tcttttcgat atcacgcggt ccaacttcaa atctcaagca tacaccttta acttcccaat ggttatattt ccagccagga gtatagttag atctatcatc gatctttact ctaaccccag ccttcttcaa aatacactcc acttcattgc agattttctt ctgttcctca gtgataacgg ttttaaaaat gatgggaata ataataactt gtacaggtgc aaccttaggt ggcaatacta aacctttatt atctccatgt gtcataatca taacaccaat agctcttgta gttaagcccc aagaagtttg atgtgcatat tctttgtttc ctttttcatc ttcaaattca acaccaaaca ttttgctaaa gttttgtcca agtaaatgag aagttgcagc ctgaacagcg cgaccaattt ctggaatgaa tccttcaatt gatttagtta tatcaccccc agggaatttt tcattctcag atttggtacc tttaactaca ggagttgcca ataaatcttc ataaatagat gcatattcat ttaaaatgat gtcaaccatt tctaaagcct cttttctagt agaatgggct gtatgtcctt cttgccataa aaattcacgt gttctgatga atggagttgg atgcttaaat tcccatctta caatggaagt ccattgatta atcttaagtg gcaaatccct atgggatcta atccatttgg caaaatatgg atacatgatt gtttcagatg ttggacggat agctattggc tcggctaaat ctgattttcc tgatttagtt acccatgcaa cttcaggaga gaatccttca acgtgatctt tttcagtttc taactttttt tgggttacaa aaatggggaa ataggcattt tgtacatcat gttgtttgat tttctgatcg aaaacgcttt gaattgtttc ccaaataaag taactccatg ggcgtaatat gtaacaccca gagatatcat agtattcaat catttcagac tttactataa cttgggaata ccagtctgca aaagaagtta ttttgtctgc tgtaattcca agcaaacttt cgttttcctt agacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHSK ENESLLGITA DKITSFADWY SQVIVKSEMI EYYDISGCYI LRPWSYFIWE TIQSVFDQKI KQHDVQNAYF PIFVTQKKLE TEKDHVEGFS PEVAWVTKSG KSDLAEPIAI RPTSETIMYP YFAKWIRSHR DLPLKINQWT SIVRWEFKHP TPFIRTREFL WQEGHTAHST RKEALEMVDI ILNEYASIYE DLLATPVVKG TKSENEKFPG GDITKSIEGF IPEIGRAVQA ATSHLLGQNF SKMFGVEFED EKGNKEYAHQ TSWGLTTRAI GVMIMTHGDN KGLVLPPKVA PVQVIIIPII FKTVITEEQK KICNEVECIL KKAGVRVKID DRSNYTPGWK YNHWEVKGVC LRFEVGPRDI EKRSVRVVVR DNMEKMDIPI SELESKIPKL LEEFQNRLLF KAKQRQNESI IRVDTFDKVM DTLNQKKMVI APWCEDVSCE EEIKKETARL SLDNEDNQSM TGAMKSLCIP NDQIFKIEEG KTKCFFCDKL AKKFTLFGRS Y
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctaagg aaaacgaaag tttgcttgga attacagcag acaaaataac ttcttttgca gactggtatt cccaagttat agtaaagtct gaaatgattg aatactatga tatctctggg tgttacatat tacgcccatg gagttacttt atttgggaaa caattcaaag cgttttcgat cagaaaatca aacaacatga tgtacaaaat gcctatttcc ccatttttgt aacccaaaaa aagttagaaa ctgaaaaaga tcacgttgaa ggattctctc ctgaagttgc atgggtaact aaatcaggaa aatcagattt agccgagcca atagctatcc gtccaacatc tgaaacaatc atgtatccat attttgccaa atggattaga tcccataggg atttgccact taagattaat caatggactt ccattgtaag atgggaattt aagcatccaa ctccattcat cagaacacgt gaatttttat ggcaagaagg acatacagcc cattctacta gaaaagaggc tttagaaatg gttgacatca ttttaaatga atatgcatct atttatgaag atttattggc aactcctgta gttaaaggta ccaaatctga gaatgaaaaa ttccctgggg gtgatataac taaatcaatt gaaggattca ttccagaaat tggtcgcgct gttcaggctg caacttctca tttacttgga caaaacttta gcaaaatgtt tggtgttgaa tttgaagatg aaaaaggaaa caaagaatat gcacatcaaa cttcttgggg cttaactaca agagctattg gtgttatgat tatgacacat ggagataata aaggtttagt attgccacct aaggttgcac ctgtacaagt tattattatt cccatcattt ttaaaaccgt tatcactgag gaacagaaga aaatctgcaa tgaagtggag tgtattttga agaaggctgg ggttagagta aagatcgatg atagatctaa ctatactcct ggctggaaat ataaccattg ggaagttaaa ggtgtatgct tgagatttga agttggaccg cgtgatatcg aaaagagaag tgttcgtgtt gttgttagag ataatatgga gaaaatggat attccaattt ccgaattgga atcaaagata ccaaagttgt tggaagaatt ccaaaataga ctgttattta aggccaaaca acgacaaaat gaatctatca taagagttga tacatttgac aaagttatgg atacactgaa ccaaaagaag atggttatag ctccttggtg tgaggatgta tcatgcgagg aagaaattaa aaaggaaact gcaagactta gtttggataa tgaagacaat cagtccatga ccggtgcaat gaaatccttg tgtataccta atgatcaaat atttaaaata gaagaaggaa aaacaaagtg tttcttctgt gataaactcg caaagaaatt tacactattt ggaagaagtt actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrpaA.18681.a.B1.PW37640
| PURIFICATION DATe: | 1/27/2015 |
| CONCENTRATION: | 24mg/ml |
| OBSERVED MW: | 80kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS VDEKLYSEVL DQLSKLSINY SKFDHDATPN MESMVEVLKE QAEKHNTDFA KNLLIKSKGN EGLFFVLAHH ETDTKMKNLG QIFGVSGNKL RLADEDVLND TLKVKRGCLT PLSLIFEKSG GIQVYFDECL KDKKVFVHPL TNTESFSIHI NDIVKFAESC GKKVKWFSMD NLEEQKPAGK PEESKENESL LGITADKITS FADWYSQVIV KSEMIEYYDI SGCYILRPWS YFIWETIQSV FDQKIKQHDV QNAYFPIFVT QKKLETEKDH VEGFSPEVAW VTKSGKSDLA EPIAIRPTSE TIMYPYFAKW IRSHRDLPLK INQWTSIVRW EFKHPTPFIR TREFLWQEGH TAHSTRKEAL EMVDIILNEY ASIYEDLLAT PVVKGTKSEN EKFPGGDITK SIEGFIPEIG RAVQAATSHL LGQNFSKMFG VEFEDEKGNK EYAHQTSWGL TTRAIGVMIM THGDNKGLVL PPKVAPVQVI IIPIIFKTVI TEEQKKICNE VECILKKAGV RVKIDDRSNY TPGWKYNHWE VKGVCLRFEV GPRDIEKRSV RVVVRDNMEK MDIPISELES KIPKLLEEFQ NRLLFKAKQR QNESIIRVDT FDKVMDTLNQ KKMVIAPWCE DVSCEEEIKK ETARLSLDNE DNQSMTGAMK SLCIPNDQIF KIEEGKTKCF FCDKLAKKFT LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG TGGATGAAAA ACTATACTCC GAGGTATTAG ATCAATTGAG CAAGCTCTCT ATAAACTATT CTAAGTTTGA TCATGATGCT ACCCCAAATA TGGAATCTAT GGTGGAAGTG CTTAAAGAGC AGGCAGAAAA ACACAATACT GACTTTGCAA AAAATCTATT AATAAAATCA AAAGGTAACG AAGGCTTATT TTTTGTTTTG GCACACCACG AAACCGATAC GAAAATGAAG AACCTTGGGC AGATCTTTGG TGTGAGTGGA AACAAGCTTA GATTAGCTGA TGAAGATGTT TTAAATGATA CTCTTAAGGT AAAAAGGGGA TGCTTAACTC CTTTATCTTT AATATTTGAG AAATCTGGAG GAATTCAAGT TTACTTTGAT GAATGTCTTA AAGACAAGAA AGTATTTGTA CATCCACTAA CCAATACTGA AAGCTTTTCA ATCCATATTA ATGACATTGT AAAATTTGCT GAATCATGTG GGAAGAAAGT TAAATGGTTT TCTATGGATA ATTTAGAGGA ACAGAAACCT GCAGGAAAGC CTGAAGAATC TAAGGAAAAC GAAAGTTTGC TTGGAATTAC AGCAGACAAA ATAACTTCTT TTGCAGACTG GTATTCCCAA GTTATAGTAA AGTCTGAAAT GATTGAATAC TATGATATCT CTGGGTGTTA CATATTACGC CCATGGAGTT ACTTTATTTG GGAAACAATT CAAAGCGTTT TCGATCAGAA AATCAAACAA CATGATGTAC AAAATGCCTA TTTCCCCATT TTTGTAACCC AAAAAAAGTT AGAAACTGAA AAAGATCACG TTGAAGGATT CTCTCCTGAA GTTGCATGGG TAACTAAATC AGGAAAATCA GATTTAGCCG AGCCAATAGC TATCCGTCCA ACATCTGAAA CAATCATGTA TCCATATTTT GCCAAATGGA TTAGATCCCA TAGGGATTTG CCACTTAAGA TTAATCAATG GACTTCCATT GTAAGATGGG AATTTAAGCA TCCAACTCCA TTCATCAGAA CACGTGAATT TTTATGGCAA GAAGGACATA CAGCCCATTC TACTAGAAAA GAGGCTTTAG AAATGGTTGA CATCATTTTA AATGAATATG CATCTATTTA TGAAGATTTA TTGGCAACTC CTGTAGTTAA AGGTACCAAA TCTGAGAATG AAAAATTCCC TGGGGGTGAT ATAACTAAAT CAATTGAAGG ATTCATTCCA GAAATTGGTC GCGCTGTTCA GGCTGCAACT TCTCATTTAC TTGGACAAAA CTTTAGCAAA ATGTTTGGTG TTGAATTTGA AGATGAAAAA GGAAACAAAG AATATGCACA TCAAACTTCT TGGGGCTTAA CTACAAGAGC TATTGGTGTT ATGATTATGA CACATGGAGA TAATAAAGGT TTAGTATTGC CACCTAAGGT TGCACCTGTA CAAGTTATTA TTATTCCCAT CATTTTTAAA ACCGTTATCA CTGAGGAACA GAAGAAAATC TGCAATGAAG TGGAGTGTAT TTTGAAGAAG GCTGGGGTTA GAGTAAAGAT CGATGATAGA TCTAACTATA CTCCTGGCTG GAAATATAAC CATTGGGAAG TTAAAGGTGT ATGCTTGAGA TTTGAAGTTG GACCGCGTGA TATCGAAAAG AGAAGTGTTC GTGTTGTTGT TAGAGATAAT ATGGAGAAAA TGGATATTCC AATTTCCGAA TTGGAATCAA AGATACCAAA GTTGTTGGAA GAATTCCAAA ATAGACTGTT ATTTAAGGCC AAACAACGAC AAAATGAATC TATCATAAGA GTTGATACAT TTGACAAAGT TATGGATACA CTGAACCAAA AGAAGATGGT TATAGCTCCT TGGTGTGAGG ATGTATCATG CGAGGAAGAA ATTAAAAAGG AAACTGCAAG ACTTAGTTTG GATAATGAAG ACAATCAGTC CATGACCGGT GCAATGAAAT CCTTGTGTAT ACCTAATGAT CAAATATTTA AAATAGAAGA AGGAAAAACA AAGTGTTTCT TCTGTGATAA ACTCGCAAAG AAATTTACAC TATTTGGAAG AAGTTACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT