CrpaA.18259.a

DNA polymerase delta small subunit, putative

CENTER ID: CrpaA.18259.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.18259.a.B1.GE35462 full length 1 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.18259.a.B1.PW36122 full length 1 475

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_1620
RefSeq: XP_627069.1
UniProt: Q5CW50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLEKDVKSL KRPEVDIKNE CERFRVKDYS FPHQYCHLCF SRFMSLRPFL LKAVQRKWKC HLSVEQEKVE KIASQGNPED RSIPILNELK DMKIESECII MGFVTKEMKN RPTILDEYLQ DIEEATKNSQ LSNYTSNDDK VYIEDSSSRI PLTLKEDVFS MNNLISGTIL AVRGVQNSGG NFLVSDLTFA SPPEEIIGKE YTLGKSSQPV YLAFVSGLNI GSEIENSLSL QLFRDFIMGV SSYSSEHKLL SSQIAYLIIA GNIIRLLDTA KETVTGTGTK LVVNNELLSS RLGVVDSFIS QLASTISLAI MPGEDDPVPI SLPQPPFQPY IFKNSRNYQS FNSFTNPCLF SINNVRVTGV SGQCISKISL FSSYSKPIDA LKFCVESRNI APTCPDVIPT YPFFDRDPFA LDYTDESFPQ VIFAGNQSEF GSYRFPNNGP LCFTIPEFSN NPTLVLLDIN SYELKTISFK TSLSI
NT Sequence
atgaaattag aaaaagatgt taagagcctg aaacggccgg aagtagatat taagaatgaa tgtgaacgat ttagagtaaa agattacagc tttccacacc agtactgcca cttatgtttt tcaaggttca tgagtttgcg tccattttta ttgaaagcag tacagagaaa gtggaagtgc catctttcag tagaacagga aaaagttgag aagattgcaa gtcagggtaa ccctgaggat agatcgatac ctattttgaa tgagcttaaa gatatgaaaa ttgagtctga atgcattatc atggggtttg tgacaaagga aatgaaaaat agaccaacaa ttttggatga gtatcttcaa gatatcgaag aggctacaaa gaacagtcaa ttaagcaatt atactagtaa tgatgacaag gtttatatag aggatagttc atcaagaatt ccattgactc taaaagaaga tgtattttca atgaataatt tgatttctgg taccatacta gcagtaaggg gagtacaaaa ttcagggggc aactttctag tctcggattt gacttttgcc tctcctcctg aagagattat tggaaaagaa tatacattag ggaagtcttc tcagccagta tatttggctt ttgtttccgg tttaaatata ggatcagaaa tagagaattc attatcttta caattatttc gtgatttcat tatgggagtt tcttcatata gtagcgaaca caaactttta tcatcacaaa ttgcttactt aattatagca ggaaacatta ttagactttt agatactgca aaagagactg ttactggtac tggaaccaag ttagttgtaa ataatgagtt gctttcaagc aggcttggtg tggttgattc ctttatttct cagctggctt ccactatatc tcttgcgatc atgcctggag aggatgatcc tgttccaatt tctcttcctc aacctccttt ccagccttat atattcaaaa attcaagaaa ctatcagtcg tttaattctt tcacaaatcc ttgcttgttt tctattaaca atgtaagagt aactggtgtt tctggccaat gtatttcaaa gatttctcta ttttcttcat attcgaagcc tattgatgct ctcaaatttt gtgttgaatc aagaaatatt gctcctacat gccctgatgt aatacctact tacccattct tcgatcgtga tccttttgct ttggattata ccgatgaatc ttttccacaa gtcatttttg caggaaatca atcagaattt gggtcatata gattccctaa taatggacca ttatgcttca ccattcctga gttctctaat aaccctactt tggtgctttt agatataaat tcatatgaac tcaaaacaat atcttttaaa acaagtcttt caatataa
Details for CrpaA.18259.a.B1.GE35462
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 54kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MSLRPFLLKA VQRKWKCHLS VEQEKVEKIA SQGNPEDRSI PILNELKDMK IESECIIMGF VTKEMKNRPT ILDEYLQDIE EATKNSQLSN YTSNDDKVYI EDSSSRIPLT LKEDVFSMNN LISGTILAVR GVQNSGGNFL VSDLTFASPP EEIIGKEYTL GKSSQPVYLA FVSGLNIGSE IENSLSLQLF RDFIMGVSSY SSEHKLLSSQ IAYLIIAGNI IRLLDTAKET VTGTGTKLVV NNELLSSRLG VVDSFISQLA STISLAIMPG EDDPVPISLP QPPFQPYIFK NSRNYQSFNS FTNPCLFSIN NVRVTGVSGQ CISKISLFSS YSKPIDALKF CVESRNIAPT CPDVIPTYPF FDRDPFALDY TDESFPQVIF AGNQSEFGSY RFPNNGPLCF TIPEFSNNPT LVLLDINSYE LKT
Validated NT Sequence
attgttttga gttcatatga atttatatct aaaagcacca aagtagggtt attagagaac tcaggaatgg tgaagcataa tggtccatta ttagggaatc tatatgaccc aaattctgat tgatttcctg caaaaatgac ttgtggaaaa gattcatcgg tataatccaa agcaaaagga tcacgatcga agaatgggta agtaggtatt acatcagggc atgtaggagc aatatttctt gattcaacac aaaatttgag agcatcaata ggcttcgaat atgaagaaaa tagagaaatc tttgaaatac attggccaga aacaccagtt actcttacat tgttaataga aaacaagcaa ggatttgtga aagaattaaa cgactgatag tttcttgaat ttttgaatat ataaggctgg aaaggaggtt gaggaagaga aattggaaca ggatcatcct ctccaggcat gatcgcaaga gatatagtgg aagccagctg agaaataaag gaatcaacca caccaagcct gcttgaaagc aactcattat ttacaactaa cttggttcca gtaccagtaa cagtctcttt tgcagtatct aaaagtctaa taatgtttcc tgctataatt aagtaagcaa tttgtgatga taaaagtttg tgttcgctac tatatgaaga aactcccata atgaaatcac gaaataattg taaagataat gaattctcta tttctgatcc tatatttaaa ccggaaacaa aagccaaata tactggctga gaagacttcc ctaatgtata ttcttttcca ataatctctt caggaggaga ggcaaaagtc aaatccgaga ctagaaagtt gccccctgaa ttttgtactc cccttactgc tagtatggta ccagaaatca aattattcat tgaaaataca tcttctttta gagtcaatgg aattcttgat gaactatcct ctatataaac cttgtcatca ttactagtat aattgcttaa ttgactgttc tttgtagcct cttcgatatc ttgaagatac tcatccaaaa ttgttggtct atttttcatt tcctttgtca caaaccccat gataatgcat tcagactcaa ttttcatatc tttaagctca ttcaaaatag gtatcgatct atcctcaggg ttaccctgac ttgcaatctt ctcaactttt tcctgttcta ctgaaagatg gcacttccac tttctctgta ctgctttcaa taaaaatgga cgcaaactca tgaaccttga aaaacataag tggcagtact ggtgtggaaa gctgtaatct tttactctaa atcgttcaca ttcattctta atatctactt ccggccgttt caggctctta acatcttttt ctaa
Expected Protein Sequence
MAHHHHHHMK LEKDVKSLKR PEVDIKNECE RFRVKDYSFP HQYCHLCFSR FMSLRPFLLK AVQRKWKCHL SVEQEKVEKI ASQGNPEDRS IPILNELKDM KIESECIIMG FVTKEMKNRP TILDEYLQDI EEATKNSQLS NYTSNDDKVY IEDSSSRIPL TLKEDVFSMN NLISGTILAV RGVQNSGGNF LVSDLTFASP PEEIIGKEYT LGKSSQPVYL AFVSGLNIGS EIENSLSLQL FRDFIMGVSS YSSEHKLLSS QIAYLIIAGN IIRLLDTAKE TVTGTGTKLV VNNELLSSRL GVVDSFISQL ASTISLAIMP GEDDPVPISL PQPPFQPYIF KNSRNYQSFN SFTNPCLFSI NNVRVTGVSG QCISKISLFS SYSKPIDALK FCVESRNIAP TCPDVIPTYP FFDRDPFALD YTDESFPQVI FAGNQSEFGS YRFPNNGPLC FTIPEFSNNP TLVLLDINSY ELKTISFKTS LSI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tagaaaaaga tgttaagagc ctgaaacggc cggaagtaga tattaagaat gaatgtgaac gatttagagt aaaagattac agctttccac accagtactg ccacttatgt ttttcaaggt tcatgagttt gcgtccattt ttattgaaag cagtacagag aaagtggaag tgccatcttt cagtagaaca ggaaaaagtt gagaagattg caagtcaggg taaccctgag gatagatcga tacctatttt gaatgagctt aaagatatga aaattgagtc tgaatgcatt atcatggggt ttgtgacaaa ggaaatgaaa aatagaccaa caattttgga tgagtatctt caagatatcg aagaggctac aaagaacagt caattaagca attatactag taatgatgac aaggtttata tagaggatag ttcatcaaga attccattga ctctaaaaga agatgtattt tcaatgaata atttgatttc tggtaccata ctagcagtaa ggggagtaca aaattcaggg ggcaactttc tagtctcgga tttgactttt gcctctcctc ctgaagagat tattggaaaa gaatatacat tagggaagtc ttctcagcca gtatatttgg cttttgtttc cggtttaaat ataggatcag aaatagagaa ttcattatct ttacaattat ttcgtgattt cattatggga gtttcttcat atagtagcga acacaaactt ttatcatcac aaattgctta cttaattata gcaggaaaca ttattagact tttagatact gcaaaagaga ctgttactgg tactggaacc aagttagttg taaataatga gttgctttca agcaggcttg gtgtggttga ttcctttatt tctcagctgg cttccactat atctcttgcg atcatgcctg gagaggatga tcctgttcca atttctcttc ctcaacctcc tttccagcct tatatattca aaaattcaag aaactatcag tcgtttaatt ctttcacaaa tccttgcttg ttttctatta acaatgtaag agtaactggt gtttctggcc aatgtatttc aaagatttct ctattttctt catattcgaa gcctattgat gctctcaaat tttgtgttga atcaagaaat attgctccta catgccctga tgtaatacct acttacccat tcttcgatcg tgatcctttt gctttggatt ataccgatga atcttttcca caagtcattt ttgcaggaaa tcaatcagaa tttgggtcat atagattccc taataatgga ccattatgct tcaccattcc tgagttctct aataacccta ctttggtgct tttagatata aattcatatg aactcaaaac aatatctttt aaaacaagtc tttcaatatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrpaA.18259.a.B1.PW36122
PURIFICATION DATe: 9/11/2012
CONCENTRATION: 19.43mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Protocol Notes
notes unavailable
Validated AA Sequence
MSLRPFLLKA VQRKWKCHLS VEQEKVEKIA SQGNPEDRSI PILNELKDMK IESECIIMGF VTKEMKNRPT ILDEYLQDIE EATKNSQLSN YTSNDDKVYI EDSSSRIPLT LKEDVFSMNN LISGTILAVR GVQNSGGNFL VSDLTFASPP EEIIGKEYTL GKSSQPVYLA FVSGLNIGSE IENSLSLQLF RDFIMGVSSY SSEHKLLSSQ IAYLIIAGNI IRLLDTAKET VTGTGTKLVV NNELLSSRLG VVDSFISQLA STISLAIMPG EDDPVPISLP QPPFQPYIFK NSRNYQSFNS FTNPCLFSIN NVRVTGVSGQ CISKISLFSS YSKPIDALKF CVESRNIAPT CPDVIPTYPF FDRDPFALDY TDESFPQVIF AGNQSEFGSY RFPNNGPLCF TIPEFSNNPT LVLLDINSYE LKT
Validated NT Sequence
attgttttga gttcatatga atttatatct aaaagcacca aagtagggtt attagagaac tcaggaatgg tgaagcataa tggtccatta ttagggaatc tatatgaccc aaattctgat tgatttcctg caaaaatgac ttgtggaaaa gattcatcgg tataatccaa agcaaaagga tcacgatcga agaatgggta agtaggtatt acatcagggc atgtaggagc aatatttctt gattcaacac aaaatttgag agcatcaata ggcttcgaat atgaagaaaa tagagaaatc tttgaaatac attggccaga aacaccagtt actcttacat tgttaataga aaacaagcaa ggatttgtga aagaattaaa cgactgatag tttcttgaat ttttgaatat ataaggctgg aaaggaggtt gaggaagaga aattggaaca ggatcatcct ctccaggcat gatcgcaaga gatatagtgg aagccagctg agaaataaag gaatcaacca caccaagcct gcttgaaagc aactcattat ttacaactaa cttggttcca gtaccagtaa cagtctcttt tgcagtatct aaaagtctaa taatgtttcc tgctataatt aagtaagcaa tttgtgatga taaaagtttg tgttcgctac tatatgaaga aactcccata atgaaatcac gaaataattg taaagataat gaattctcta tttctgatcc tatatttaaa ccggaaacaa aagccaaata tactggctga gaagacttcc ctaatgtata ttcttttcca ataatctctt caggaggaga ggcaaaagtc aaatccgaga ctagaaagtt gccccctgaa ttttgtactc cccttactgc tagtatggta ccagaaatca aattattcat tgaaaataca tcttctttta gagtcaatgg aattcttgat gaactatcct ctatataaac cttgtcatca ttactagtat aattgcttaa ttgactgttc tttgtagcct cttcgatatc ttgaagatac tcatccaaaa ttgttggtct atttttcatt tcctttgtca caaaccccat gataatgcat tcagactcaa ttttcatatc tttaagctca ttcaaaatag gtatcgatct atcctcaggg ttaccctgac ttgcaatctt ctcaactttt tcctgttcta ctgaaagatg gcacttccac tttctctgta ctgctttcaa taaaaatgga cgcaaactca tgaaccttga aaaacataag tggcagtact ggtgtggaaa gctgtaatct tttactctaa atcgttcaca ttcattctta atatctactt ccggccgttt caggctctta acatcttttt ctaa
Expressed Protein Sequence
MAHHHHHHMK LEKDVKSLKR PEVDIKNECE RFRVKDYSFP HQYCHLCFSR FMSLRPFLLK AVQRKWKCHL SVEQEKVEKI ASQGNPEDRS IPILNELKDM KIESECIIMG FVTKEMKNRP TILDEYLQDI EEATKNSQLS NYTSNDDKVY IEDSSSRIPL TLKEDVFSMN NLISGTILAV RGVQNSGGNF LVSDLTFASP PEEIIGKEYT LGKSSQPVYL AFVSGLNIGS EIENSLSLQL FRDFIMGVSS YSSEHKLLSS QIAYLIIAGN IIRLLDTAKE TVTGTGTKLV VNNELLSSRL GVVDSFISQL ASTISLAIMP GEDDPVPISL PQPPFQPYIF KNSRNYQSFN SFTNPCLFSI NNVRVTGVSG QCISKISLFS SYSKPIDALK FCVESRNIAP TCPDVIPTYP FFDRDPFALD YTDESFPQVI FAGNQSEFGS YRFPNNGPLC FTIPEFSNNP TLVLLDINSY ELKTISFKTS LSI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT TAGAAAAAGA TGTTAAGAGC CTGAAACGGC CGGAAGTAGA TATTAAGAAT GAATGTGAAC GATTTAGAGT AAAAGATTAC AGCTTTCCAC ACCAGTACTG CCACTTATGT TTTTCAAGGT TCATGAGTTT GCGTCCATTT TTATTGAAAG CAGTACAGAG AAAGTGGAAG TGCCATCTTT CAGTAGAACA GGAAAAAGTT GAGAAGATTG CAAGTCAGGG TAACCCTGAG GATAGATCGA TACCTATTTT GAATGAGCTT AAAGATATGA AAATTGAGTC TGAATGCATT ATCATGGGGT TTGTGACAAA GGAAATGAAA AATAGACCAA CAATTTTGGA TGAGTATCTT CAAGATATCG AAGAGGCTAC AAAGAACAGT CAATTAAGCA ATTATACTAG TAATGATGAC AAGGTTTATA TAGAGGATAG TTCATCAAGA ATTCCATTGA CTCTAAAAGA AGATGTATTT TCAATGAATA ATTTGATTTC TGGTACCATA CTAGCAGTAA GGGGAGTACA AAATTCAGGG GGCAACTTTC TAGTCTCGGA TTTGACTTTT GCCTCTCCTC CTGAAGAGAT TATTGGAAAA GAATATACAT TAGGGAAGTC TTCTCAGCCA GTATATTTGG CTTTTGTTTC CGGTTTAAAT ATAGGATCAG AAATAGAGAA TTCATTATCT TTACAATTAT TTCGTGATTT CATTATGGGA GTTTCTTCAT ATAGTAGCGA ACACAAACTT TTATCATCAC AAATTGCTTA CTTAATTATA GCAGGAAACA TTATTAGACT TTTAGATACT GCAAAAGAGA CTGTTACTGG TACTGGAACC AAGTTAGTTG TAAATAATGA GTTGCTTTCA AGCAGGCTTG GTGTGGTTGA TTCCTTTATT TCTCAGCTGG CTTCCACTAT ATCTCTTGCG ATCATGCCTG GAGAGGATGA TCCTGTTCCA ATTTCTCTTC CTCAACCTCC TTTCCAGCCT TATATATTCA AAAATTCAAG AAACTATCAG TCGTTTAATT CTTTCACAAA TCCTTGCTTG TTTTCTATTA ACAATGTAAG AGTAACTGGT GTTTCTGGCC AATGTATTTC AAAGATTTCT CTATTTTCTT CATATTCGAA GCCTATTGAT GCTCTCAAAT TTTGTGTTGA ATCAAGAAAT ATTGCTCCTA CATGCCCTGA TGTAATACCT ACTTACCCAT TCTTCGATCG TGATCCTTTT GCTTTGGATT ATACCGATGA ATCTTTTCCA CAAGTCATTT TTGCAGGAAA TCAATCAGAA TTTGGGTCAT ATAGATTCCC TAATAATGGA CCATTATGCT TCACCATTCC TGAGTTCTCT AATAACCCTA CTTTGGTGCT TTTAGATATA AATTCATATG AACTCAAAAC AATATCTTTT AAAACAAGTC TTTCAATATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT