CrpaA.18219.a: N-myristoyltransferase
Target Characteristics
| from organism: | Cryptosporidium parvum Iowa II |
| most recent status: | in PDB |
| center reference id: | CrpaA.18219.a |
| is community request: | True |
| associated disease: | cryptosporidiosis - (diarrheal disease) |
| NIH risk group: | 2 |
| is select agent: | False |
| NIH priority pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.18219.a.A10.GE42168 | truncation | 43 | 469 | GE42168 | |
| CrpaA.18219.a.A11.GE42169 | truncation | 44 | 469 | GE42169 | |
| CrpaA.18219.a.A12.GE42170 | truncation | 45 | 469 | GE42170 | |
| CrpaA.18219.a.A3.GE39566 | truncation 2 | 84 | 469 | GE39566 | |
| CrpaA.18219.a.A4.GE39567 | truncation | 42 | 469 | GE39567 | |
| CrpaA.18219.a.A41.GE42008 | truncation | 42 | 469 | GE42008 | |
| CrpaA.18219.a.A42.GE42009 | truncation | 42 | 469 | GE42009 | |
| CrpaA.18219.a.A6.GE39568 | truncation | 92 | 469 | GE39568 | |
| CrpaA.18219.a.A7.GE39569 | truncation | 79 | 469 | GE39569 | |
| CrpaA.18219.a.A8.GE42171 | truncation | 40 | 469 | GE42171 | |
| CrpaA.18219.a.A9.GE42172 | truncation | 41 | 469 | GE42172 | |
| CrpaA.18219.a.B1.GE35467 | full length | 1 | 469 | GE35467 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.18219.a.B1.PS38190 | full length | 1 | 469 | ||
| CrpaA.18219.a.B1.PW37298 | full length | 1 | 469 | ||
| CrpaA.18219.a.A4.PW37835 | truncation | 42 | 469 | ||
| CrpaA.18219.a.A10.PW39122 | truncation | 43 | 469 |
External Resources
| Resource | Reference ID |
|---|---|
| EuPathDB: | CryptoDB:cgd3_320 |
| OrthoMCL: | OG5_127597 |
| RefSeq: | XP_626618.1 |
| UniProt: | Q5CV46 |
Enzyme & Pathway Information
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
LIRMSGETPE NSESSISNTK KITNLLKEMS LGSFMNTAAN AIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
NT Sequence
cttattagaa tgagtggtga aaccccagag aattcagaat cttccatttc aaataccaag aagatcacaa atcttctgaa agaaatgagc ttaggtagct ttatgaacac tgccgcaaat gcaatcaaac cacacaaatt ctggaatact caaccagtag ttcaaaacga tgattcttcc agtgaatatt catttggacc cattgaaatt gaaccagatt cttttagaaa agaaatatat aagctaccag atggattctc ttggtttgat tgtaatttat gggatataga atctcaagat tttgaagata cttaccaatt attaaaagac cattatgttg aagatgacga ttcccaattt agatttaatt attcaaagga gtttcttaga tgggctcttt gtgtccctgg tcaaaaaaag aactggcttg ttggagtcag agttaatgaa acaaagaaaa tggttggatt tatttccgca atcccaatca aagttagaat tcataattgc attatgaata cttctgtagt taattttctt tgcgttcata agaagcttag atctaaaaga cttgcccctg tacttattaa ggaaattaca agaagaatca gatgtgaaaa aatctttcaa tcaatttaca cttgtgggaa gaatattacc aaacctttta ctattggaac ttattggcat cgtataatta atgtaaagaa attattagaa gctggtttta ttggaatccc aagaaatatg accatgagct ctttaattaa atatcatcgt attcctgctg ataaaagaat tgaagggttt agaccctccg tagattctga tgcagaacaa atctgtaaat tattcgaaaa ttactttatg aaatataaag atgtcagcaa cgaaaccatg aataatctga taaactatga tgaaattaac cattcaaaag agcttggaaa acaagcatat atgaagctgg ataagattga agatttgcaa gacaaaatca caattcatca atgctttaat gttgaagatg ttaaacatta tttcacgaat attgataaag ttattgtaac ttatgttcga gaaaataaaa ataaagaaat tactgacttg tttagcttct ttataataga atccactgtc atcaataatg aaagatttcc aactattaat attgcatatt catacttcaa tattgcaaat acctgctcat taaaagaatt gttcaatgaa atgcttatta ctgccaaaaa taataattgt gatgctttta atacattgga tctgatgcaa aatttacaag ttattcaaga tagtaaattt attatcggta ccggtagatt aagatattat gtgtttaatt ggaagatacc tcaaatttca ccttcaaatg ttggaattat attattttag
harvested on: 12/14/2017
sequenced on: 12/6/2017
expected MW: 53kDa
observed MW: 50kDa
MW ratio: 0.94
antibiotic marker:
ampicillin
expression colonies count:
Good (10-50)
total expression level:
High Expression
soluble expression level:
Low Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 95
percent coverage: 66
MGTLEAQTQG PGSKPHKFWN TQPVVQNDDS SSEYSFGPIE IEPDSFRKEI YKLPDGFSWF DCNLWDIESQ DFEDTYQLLK DHYVEDDDSQ FRFNYSKEFL RWALCVPGQK KNWLVGVRVN ETKKMVGFIS AIPIKVRIHN CIMNTSVVNF LCVHKKLRSK RLAPVLIKEI TRRIRCEKIF QSIYTCGKNI TKPFTIGTYW HRIINVKKLL EAGFIGIPRN MTMSSLIKYH RIPADKRIEG FRPSVDSDAE QICKLFENYF MKYKDVSNET MNNLINYDEI NHXXXXXXTS IYXXGXX
ANGGNCTCAT CACCATCACC ATCATATGGG TACCCTGGAA GCTCAGACCC AGGGTCCTGG TTCGAAACCA CACAAATTCT GGAATACTCA ACCAGTAGTT CAAAACGATG ATTCTTCCAG TGAATATTCA TTTGGACCCA TTGAAATTGA ACCAGATTCT TTTAGAAAAG AAATATATAA GCTACCAGAT GGATTCTCTT GGTTTGATTG TAATTTATGG GATATAGAAT CTCAAGATTT TGAAGATACT TACCAATTAT TAAAAGACCA TTATGTTGAA GATGACGATT CCCAATTTAG ATTTAATTAT TCAAAGGAGT TTCTTAGATG GGCTCTTTGT GTCCCTGGTC AAAAAAAGAA CTGGCTTGTT GGAGTCAGAG TTAATGAAAC AAAGAAAATG GTTGGATTTA TTTCCGCAAT CCCAATCAAA GTTAGAATTC ATAATTGCAT TATGAATACT TCTGTAGTTA ATTTTCTTTG CGTTCATAAG AAGCTTAGAT CTAAAAGACT TGCCCCTGTA CTTATTAAGG AAATTACAAG AAGAATCAGA TGTGAAAAAA TCTTTCAATC AATTTACACT TGTGGGAAGA ATATTACCAA ACCTTTTACT ATTGGAACTT ATTGGCATCG TATAATTAAT GTAAAGAAAT TATTAGAAGC TGGTTTTATT GGAATCCCAA GAAATATGAC CATGAGCTCT TTAATTAAAT ATCATCGTAT TCCTGCTGAT AAAAGAATTG AAGGGTTTAG ACCCTCCGTA GATTCTGATG CAGAACAAAT CTGTAAATTA TTCGAAAATT ACTTTATGAA ATATAAAGAT GTCAGCAACG AAACCATGAA TAATCTGATA AACTATGATG AAATTAACCA TNNNNANNNN CNNNNGNAAA CAAGCATATA TNNNNNTGGA NNNNNTTGAN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ATNNNNNNNN NNNNNNNNNN NNAANNNNNA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA ANNNNNNNNN NNT
MAHHHHHHMG TLEAQTQGPG SKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAA ACCACACAAA TTCTGGAATA CTCAACCAGT AGTTCAAAAC GATGATTCTT CCAGTGAATA TTCATTTGGA CCCATTGAAA TTGAACCAGA TTCTTTTAGA AAAGAAATAT ATAAGCTACC AGATGGATTC TCTTGGTTTG ATTGTAATTT ATGGGATATA GAATCTCAAG ATTTTGAAGA TACTTACCAA TTATTAAAAG ACCATTATGT TGAAGATGAC GATTCCCAAT TTAGATTTAA TTATTCAAAG GAGTTTCTTA GATGGGCTCT TTGTGTCCCT GGTCAAAAAA AGAACTGGCT TGTTGGAGTC AGAGTTAATG AAACAAAGAA AATGGTTGGA TTTATTTCCG CAATCCCAAT CAAAGTTAGA ATTCATAATT GCATTATGAA TACTTCTGTA GTTAATTTTC TTTGCGTTCA TAAGAAGCTT AGATCTAAAA GACTTGCCCC TGTACTTATT AAGGAAATTA CAAGAAGAAT CAGATGTGAA AAAATCTTTC AATCAATTTA CACTTGTGGG AAGAATATTA CCAAACCTTT TACTATTGGA ACTTATTGGC ATCGTATAAT TAATGTAAAG AAATTATTAG AAGCTGGTTT TATTGGAATC CCAAGAAATA TGACCATGAG CTCTTTAATT AAATATCATC GTATTCCTGC TGATAAAAGA ATTGAAGGGT TTAGACCCTC CGTAGATTCT GATGCAGAAC AAATCTGTAA ATTATTCGAA AATTACTTTA TGAAATATAA AGATGTCAGC AACGAAACCA TGAATAATCT GATAAACTAT GATGAAATTA ACCATTCAAA AGAGCTTGGA AAACAAGCAT ATATGAAGCT GGATAAGATT GAAGATTTGC AAGACAAAAT CACAATTCAT CAATGCTTTA ATGTTGAAGA TGTTAAACAT TATTTCACGA ATATTGATAA AGTTATTGTA ACTTATGTTC GAGAAAATAA AAATAAAGAA ATTACTGACT TGTTTAGCTT CTTTATAATA GAATCCACTG TCATCAATAA TGAAAGATTT CCAACTATTA ATATTGCATA TTCATACTTC AATATTGCAA ATACCTGCTC ATTAAAAGAA TTGTTCAATG AAATGCTTAT TACTGCCAAA AATAATAATT GTGATGCTTT TAATACATTG GATCTGATGC AAAATTTACA AGTTATTCAA GATAGTAAAT TTATTATCGG TACCGGTAGA TTAAGATATT ATGTGTTTAA TTGGAAGATA CCTCAAATTT CACCTTCAAA TGTTGGAATT ATATTATTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
harvested on: 1/8/2018
sequenced on: 12/12/2017
expected MW: 52kDa
observed MW: 52kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Good (10-50)
total expression level:
High Expression
soluble expression level:
Moderate Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 86
percent coverage: 75
MGTLEAQTQG PGSPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELXXQAY MKLXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
ANGGNCTCAT CACCATCACC ATCATATGGG TACCCTGGAA GCTCAGACCC AGGGTCCTGG TTCGCCACAC AAATTCTGGA ATACTCAACC AGTAGTTCAA AACGATGATT CTTCCAGTGA ATATTCATTT GGACCCATTG AAATTGAACC AGATTCTTTT AGAAAAGAAA TATATAAGCT ACCAGATGGA TTCTCTTGGT TTGATTGTAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATAAA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AAAAGAGCTN NNNNNACAAG CATATATGAA GCTGGNNNNN NNTGANNNNN NNNANNNNNA NATNNNNNNN NNTCANNNCN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNA
MAHHHHHHMG TLEAQTQGPG SPHKFWNTQP VVQNDDSSSE YSFGPIEIEP DSFRKEIYKL PDGFSWFDCN LWDIESQDFE DTYQLLKDHY VEDDDSQFRF NYSKEFLRWA LCVPGQKKNW LVGVRVNETK KMVGFISAIP IKVRIHNCIM NTSVVNFLCV HKKLRSKRLA PVLIKEITRR IRCEKIFQSI YTCGKNITKP FTIGTYWHRI INVKKLLEAG FIGIPRNMTM SSLIKYHRIP ADKRIEGFRP SVDSDAEQIC KLFENYFMKY KDVSNETMNN LINYDEINHS KELGKQAYMK LDKIEDLQDK ITIHQCFNVE DVKHYFTNID KVIVTYVREN KNKEITDLFS FFIIESTVIN NERFPTINIA YSYFNIANTC SLKELFNEML ITAKNNNCDA FNTLDLMQNL QVIQDSKFII GTGRLRYYVF NWKIPQISPS NVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCC ACACAAATTC TGGAATACTC AACCAGTAGT TCAAAACGAT GATTCTTCCA GTGAATATTC ATTTGGACCC ATTGAAATTG AACCAGATTC TTTTAGAAAA GAAATATATA AGCTACCAGA TGGATTCTCT TGGTTTGATT GTAATTTATG GGATATAGAA TCTCAAGATT TTGAAGATAC TTACCAATTA TTAAAAGACC ATTATGTTGA AGATGACGAT TCCCAATTTA GATTTAATTA TTCAAAGGAG TTTCTTAGAT GGGCTCTTTG TGTCCCTGGT CAAAAAAAGA ACTGGCTTGT TGGAGTCAGA GTTAATGAAA CAAAGAAAAT GGTTGGATTT ATTTCCGCAA TCCCAATCAA AGTTAGAATT CATAATTGCA TTATGAATAC TTCTGTAGTT AATTTTCTTT GCGTTCATAA GAAGCTTAGA TCTAAAAGAC TTGCCCCTGT ACTTATTAAG GAAATTACAA GAAGAATCAG ATGTGAAAAA ATCTTTCAAT CAATTTACAC TTGTGGGAAG AATATTACCA AACCTTTTAC TATTGGAACT TATTGGCATC GTATAATTAA TGTAAAGAAA TTATTAGAAG CTGGTTTTAT TGGAATCCCA AGAAATATGA CCATGAGCTC TTTAATTAAA TATCATCGTA TTCCTGCTGA TAAAAGAATT GAAGGGTTTA GACCCTCCGT AGATTCTGAT GCAGAACAAA TCTGTAAATT ATTCGAAAAT TACTTTATGA AATATAAAGA TGTCAGCAAC GAAACCATGA ATAATCTGAT AAACTATGAT GAAATTAACC ATTCAAAAGA GCTTGGAAAA CAAGCATATA TGAAGCTGGA TAAGATTGAA GATTTGCAAG ACAAAATCAC AATTCATCAA TGCTTTAATG TTGAAGATGT TAAACATTAT TTCACGAATA TTGATAAAGT TATTGTAACT TATGTTCGAG AAAATAAAAA TAAAGAAATT ACTGACTTGT TTAGCTTCTT TATAATAGAA TCCACTGTCA TCAATAATGA AAGATTTCCA ACTATTAATA TTGCATATTC ATACTTCAAT ATTGCAAATA CCTGCTCATT AAAAGAATTG TTCAATGAAA TGCTTATTAC TGCCAAAAAT AATAATTGTG ATGCTTTTAA TACATTGGAT CTGATGCAAA ATTTACAAGT TATTCAAGAT AGTAAATTTA TTATCGGTAC CGGTAGATTA AGATATTATG TGTTTAATTG GAAGATACCT CAAATTTCAC CTTCAAATGT TGGAATTATA TTATTTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
harvested on: 12/14/2017
sequenced on: 12/6/2017
expected MW: 52kDa
observed MW: 52kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Good (10-50)
total expression level:
High Expression
soluble expression level:
Moderate Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 100
percent coverage: 98
MGTLEAQTQG PGSHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
GGNCTCATCA CCATCACCAT CATATGGGTA CCCTGGAAGC TCAGACCCAG GGTCCTGGTT CGCACAAATT CTGGAATACT CAACCAGTAG TTCAAAACGA TGATTCTTCC AGTGAATATT CATTTGGACC CATTGAAATT GAACCAGATT CTTTTAGAAA AGAAATATAT AAGCTACCAG ATGGATTCTC TTGGTTTGAT TGTAATTTAT GGGATATAGA ATCTCAAGAT TTTGAAGATA CTTACCAATT ATTAAAAGAC CATTATGTTG AAGATGACGA TTCCCAATTT AGATTTAATT ATTCAAAGGA GTTTCTTAGA TGGGCTCTTT GTGTCCCTGG TCAAAAAAAG AACTGGCTTG TTGGAGTCAG AGTTAATGAA ACAAAGAAAA TGGTTGGATT TATTTCCGCA ATCCCAATCA AAGTTAGAAT TCATAATTGC ATTATGAATA CTTCTGTAGT TAATTTTCTT TGCGTTCATA AGAAGCTTAG ATCTAAAAGA CTTGCCCCTG TACTTATTAA GGAAATTACA AGAAGAATCA GATGTGAAAA AATCTTTCAA TCAATTTACA CTTGTGGGAA GAATATTACC AAACCTTTTA CTATTGGAAC TTATTGGCAT CGTATAATTA ATGTAAAGAA ATTATTAGAA GCTGGTTTTA TTGGAATCCC AAGAAATATG ACCATGAGCT CTTTAATTAA ATATCATCGT ATTCCTGCTG ATAAAAGAAT TGAAGGGTTT AGACCCTCCG TAGATTCTGA TGCAGAACAA ATCTGTAAAT TATTCGAAAA TTACTTTATG AAATATAAAG ATGTCAGCAA CGAAACCATG AATAATCTGA TAAACTATGA TGAAATTAAC CATTCAAAAG AGCTTGGAAA ACAAGCATAT ATGAAGCTGG ATAAGATTGA AGATTTGCAA GACAAAATCA CAATTCATCA ATGCTTTAAT GTTGAAGATG TTAAACATTA TTTCACGAAT ATTGATAAAG TTATTGTAAC TTATGTTCGA GAAAATAAAA ATAAAGAAAT TACTGACTTG TTTAGCTTCT TTATAATAGA ATCCACTGTC ATCAATAATG AAAGATTTCC AACTATTAAT ATTGCATATT CATACTTCAA TATTGCAAAT ACCTGCTCAT TAAAAGAATT GTTCAATGAA ATGCTTATTA CTGCCAAAAA TAATAATTGT GATGCTTTTA ATACATTGGA TCTGATGCAA AATTTACAAG TTATTCAAGA TAGTAAATTT ATTATCGGTA CCGGTAGATT AAGATATTAT GTGTTTAATT GGAAGATACC TCAAATTTCA CCTTCAAATG TTGGAATTAT ATTATTTTGA AA
MAHHHHHHMG TLEAQTQGPG SHKFWNTQPV VQNDDSSSEY SFGPIEIEPD SFRKEIYKLP DGFSWFDCNL WDIESQDFED TYQLLKDHYV EDDDSQFRFN YSKEFLRWAL CVPGQKKNWL VGVRVNETKK MVGFISAIPI KVRIHNCIMN TSVVNFLCVH KKLRSKRLAP VLIKEITRRI RCEKIFQSIY TCGKNITKPF TIGTYWHRII NVKKLLEAGF IGIPRNMTMS SLIKYHRIPA DKRIEGFRPS VDSDAEQICK LFENYFMKYK DVSNETMNNL INYDEINHSK ELGKQAYMKL DKIEDLQDKI TIHQCFNVED VKHYFTNIDK VIVTYVRENK NKEITDLFSF FIIESTVINN ERFPTINIAY SYFNIANTCS LKELFNEMLI TAKNNNCDAF NTLDLMQNLQ VIQDSKFIIG TGRLRYYVFN WKIPQISPSN VGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCA CAAATTCTGG AATACTCAAC CAGTAGTTCA AAACGATGAT TCTTCCAGTG AATATTCATT TGGACCCATT GAAATTGAAC CAGATTCTTT TAGAAAAGAA ATATATAAGC TACCAGATGG ATTCTCTTGG TTTGATTGTA ATTTATGGGA TATAGAATCT CAAGATTTTG AAGATACTTA CCAATTATTA AAAGACCATT ATGTTGAAGA TGACGATTCC CAATTTAGAT TTAATTATTC AAAGGAGTTT CTTAGATGGG CTCTTTGTGT CCCTGGTCAA AAAAAGAACT GGCTTGTTGG AGTCAGAGTT AATGAAACAA AGAAAATGGT TGGATTTATT TCCGCAATCC CAATCAAAGT TAGAATTCAT AATTGCATTA TGAATACTTC TGTAGTTAAT TTTCTTTGCG TTCATAAGAA GCTTAGATCT AAAAGACTTG CCCCTGTACT TATTAAGGAA ATTACAAGAA GAATCAGATG TGAAAAAATC TTTCAATCAA TTTACACTTG TGGGAAGAAT ATTACCAAAC CTTTTACTAT TGGAACTTAT TGGCATCGTA TAATTAATGT AAAGAAATTA TTAGAAGCTG GTTTTATTGG AATCCCAAGA AATATGACCA TGAGCTCTTT AATTAAATAT CATCGTATTC CTGCTGATAA AAGAATTGAA GGGTTTAGAC CCTCCGTAGA TTCTGATGCA GAACAAATCT GTAAATTATT CGAAAATTAC TTTATGAAAT ATAAAGATGT CAGCAACGAA ACCATGAATA ATCTGATAAA CTATGATGAA ATTAACCATT CAAAAGAGCT TGGAAAACAA GCATATATGA AGCTGGATAA GATTGAAGAT TTGCAAGACA AAATCACAAT TCATCAATGC TTTAATGTTG AAGATGTTAA ACATTATTTC ACGAATATTG ATAAAGTTAT TGTAACTTAT GTTCGAGAAA ATAAAAATAA AGAAATTACT GACTTGTTTA GCTTCTTTAT AATAGAATCC ACTGTCATCA ATAATGAAAG ATTTCCAACT ATTAATATTG CATATTCATA CTTCAATATT GCAAATACCT GCTCATTAAA AGAATTGTTC AATGAAATGC TTATTACTGC CAAAAATAAT AATTGTGATG CTTTTAATAC ATTGGATCTG ATGCAAAATT TACAAGTTAT TCAAGATAGT AAATTTATTA TCGGTACCGG TAGATTAAGA TATTATGTGT TTAATTGGAA GATACCTCAA ATTTCACCTT CAAATGTTGG AATTATATTA TTTTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
harvested on: 10/23/2015
sequenced on: 10/23/2015
expected MW: 48kDa
observed MW: 48kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Too many to count (100+)
total expression level:
Moderate Expression
soluble expression level:
Low Expression
expression host:
data unavailable
sequencing result:
percent identity: data unavailable
percent coverage: data unavailable
MAHHHHHHMG TLEAQTQGPG SDGFSWFDCN LWDIESQDFE DTYQLLKDHY VEDDDSQFRF NYSKEFLRWA LCVPGQKKNW LVGVRVNETK KMVGFISAIP IKVRIHNCIM NTSVVNFLCV HKKLRSKRLA PVLIKEITRR IRCEKIFQSI YTCGKNITKP FTIGTYWHRI INVKKLLEAG FIGIPRNMTM SSLIKYHRIP ADKRIEGFRP SVDSDAEQIC KLFENYFMKY KDVSNETMNN LINYDEINHS KELGKQAYMK LDKIEDLQDK ITIHQCFNVE DVKHYFTNID KVIVTYVREN KNKEITDLFS FFIIESTVIN NERFPTINIA YSYFNIANTC SLKELFNEML ITAKNNNCDA FNTLDLMQNL QVIQDSKFII GTGRLRYYVF NWKIPQISPS NVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGA TGGATTCTCT TGGTTTGATT GTAATTTATG GGATATAGAA TCTCAAGATT TTGAAGATAC TTACCAATTA TTAAAAGACC ATTATGTTGA AGATGACGAT TCCCAATTTA GATTTAATTA TTCAAAGGAG TTTCTTAGAT GGGCTCTTTG TGTCCCTGGT CAAAAAAAGA ACTGGCTTGT TGGAGTCAGA GTTAATGAAA CAAAGAAAAT GGTTGGATTT ATTTCCGCAA TCCCAATCAA AGTTAGAATT CATAATTGCA TTATGAATAC TTCTGTAGTT AATTTTCTTT GCGTTCATAA GAAGCTTAGA TCTAAAAGAC TTGCCCCTGT ACTTATTAAG GAAATTACAA GAAGAATCAG ATGTGAAAAA ATCTTTCAAT CAATTTACAC TTGTGGGAAG AATATTACCA AACCTTTTAC TATTGGAACT TATTGGCATC GTATAATTAA TGTAAAGAAA TTATTAGAAG CTGGTTTTAT TGGAATCCCA AGAAATATGA CCATGAGCTC TTTAATTAAA TATCATCGTA TTCCTGCTGA TAAAAGAATT GAAGGGTTTA GACCCTCCGT AGATTCTGAT GCAGAACAAA TCTGTAAATT ATTCGAAAAT TACTTTATGA AATATAAAGA TGTCAGCAAC GAAACCATGA ATAATCTGAT AAACTATGAT GAAATTAACC ATTCAAAAGA GCTTGGAAAA CAAGCATATA TGAAGCTGGA TAAGATTGAA GATTTGCAAG ACAAAATCAC AATTCATCAA TGCTTTAATG TTGAAGATGT TAAACATTAT TTCACGAATA TTGATAAAGT TATTGTAACT TATGTTCGAG AAAATAAAAA TAAAGAAATT ACTGACTTGT TTAGCTTCTT TATAATAGAA TCCACTGTCA TCAATAATGA AAGATTTCCA ACTATTAATA TTGCATATTC ATACTTCAAT ATTGCAAATA CCTGCTCATT AAAAGAATTG TTCAATGAAA TGCTTATTAC TGCCAAAAAT AATAATTGTG ATGCTTTTAA TACATTGGAT CTGATGCAAA ATTTACAAGT TATTCAAGAT AGTAAATTTA TTATCGGTAC CGGTAGATTA AGATATTATG TGTTTAATTG GAAGATACCT CAAATTTCAC CTTCAAATGT TGGAATTATA TTATTTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
harvested on: 10/23/2015
sequenced on: 10/23/2015
expected MW: 53kDa
observed MW: 53kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Too many to count (100+)
total expression level:
Moderate Expression
soluble expression level:
Moderate Expression
expression host:
data unavailable
sequencing result:
percent identity: data unavailable
percent coverage: data unavailable
MAHHHHHHMG TLEAQTQGPG SIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT CAAACCACAC AAATTCTGGA ATACTCAACC AGTAGTTCAA AACGATGATT CTTCCAGTGA ATATTCATTT GGACCCATTG AAATTGAACC AGATTCTTTT AGAAAAGAAA TATATAAGCT ACCAGATGGA TTCTCTTGGT TTGATTGTAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATAAA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AAAAGAGCTT GGAAAACAAG CATATATGAA GCTGGATAAG ATTGAAGATT TGCAAGACAA AATCACAATT CATCAATGCT TTAATGTTGA AGATGTTAAA CATTATTTCA CGAATATTGA TAAAGTTATT GTAACTTATG TTCGAGAAAA TAAAAATAAA GAAATTACTG ACTTGTTTAG CTTCTTTATA ATAGAATCCA CTGTCATCAA TAATGAAAGA TTTCCAACTA TTAATATTGC ATATTCATAC TTCAATATTG CAAATACCTG CTCATTAAAA GAATTGTTCA ATGAAATGCT TATTACTGCC AAAAATAATA ATTGTGATGC TTTTAATACA TTGGATCTGA TGCAAAATTT ACAAGTTATT CAAGATAGTA AATTTATTAT CGGTACCGGT AGATTAAGAT ATTATGTGTT TAATTGGAAG ATACCTCAAA TTTCACCTTC AAATGTTGGA ATTATATTAT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
harvested on: 7/27/2017
sequenced on: 7/27/2017
expected MW: 53kDa
observed MW: 53kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Many (50-100)
total expression level:
High Expression
soluble expression level:
Moderate Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 100
percent coverage: 98
MGTLEAQTQG PGSMIKPHKF WNTQPVVQND DSSSEYSFGP IEIEPDSFRK EIYKLPDGFS WFDCNLWDIE SQDFEDTYQL LKDHYVEDDD SQFRFNYSKE FLRWALCVPG QKKNWLVGVR VNETKKMVGF ISAIPIKVRI HNCIMNTSVV NFLCVHKKLR SKRLAPVLIK EITRRIRCEK IFQSIYTCGK NITKPFTIGT YWHRIINVKK LLEAGFIGIP RNMTMSSLIK YHRIPADARI EGFRPSVDSD AEQICKLFEN YFMKYKDVSN ETMNNLINYD EINHSKELGK QAYMKLDKIE DLQDKITIHQ CFNVEDVKHY FTNIDKVIVT YVRENKNKEI TDLFSFFIIE STVINNERFP TINIAYSYFN IANTCSLKEL FNEMLITAKN NNCDAFNTLD LMQNLQVIQD SKFIIGTGRL RYYVFNWKIP QISPSNVGII LF
ATTAAAATAA TATAATTCCA ACATTTGAAG GTGAAATTTG AGGTATCTTC CAATTAAACA CATAATATCT TAATCTACCG GTACCGATAA TAAATTTACT ATCTTGAATA ACTTGTAAAT TTTGCATCAG ATCCAATGTA TTAAAAGCAT CACAATTATT ATTTTTGGCA GTAATAAGCA TTTCATTGAA CAATTCTTTT AATGAGCAGG TATTTGCAAT ATTGAAGTAT GAATATGCAA TATTAATAGT TGGAAATCTT TCATTATTGA TGACAGTGGA TTCTATTATA AAGAAGCTAA ACAAGTCAGT AATTTCTTTA TTTTTATTTT CTCGAACATA AGTTACAATA ACTTTATCAA TATTCGTGAA ATAATGTTTA ACATCTTCAA CATTAAAGCA TTGATGAATT GTGATTTTGT CTTGCAAATC TTCAATCTTA TCCAGCTTCA TATATGCTTG TTTTCCAAGC TCTTTTGAAT GGTTAATTTC ATCATAGTTT ATCAGATTAT TCATGGTTTC GTTGCTGACA TCTTTATATT TCATAAAGTA ATTTTCGAAT AATTTACAGA TTTGTTCTGC ATCAGAATCT ACGGAGGGTC TAAACCCTTC AATTCTTGCA TCAGCAGGAA TACGATGATA TTTAATTAAA GAGCTCATGG TCATATTTCT TGGGATTCCA ATAAAACCAG CTTCTAATAA TTTCTTTACA TTAATTATAC GATGCCAATA AGTTCCAATA GTAAAAGGTT TGGTAATATT CTTCCCACAA GTGTAAATTG ATTGAAAGAT TTTTTCACAT CTGATTCTTC TTGTAATTTC CTTAATAAGT ACAGGGGCAA GTCTTTTAGA TCTAAGCTTC TTATGAACGC AAAGAAAATT AACTACAGAA GTATTCATAA TGCAATTATG AATTCTAACT TTGATTGGGA TTGCGGAAAT AAATCCAACC ATTTTCTTTG TTTCATTAAC TCTGACTCCA ACAAGCCAGT TCTTTTTTTG ACCAGGGACA CAAAGAGCCC ATCTAAGAAA CTCCTTTGAA TAATTAAATC TAAATTGGGA ATCGTCATCT TCAACATAAT GGTCTTTTAA TAATTGGTAA GTATCTTCAA AATCTTGAGA TTCTATATCC CATAAATTAC AATCAAACCA AGAGAATCCA TCTGGTAGCT TATATATTTC TTTTCTAAAA GAATCTGGTT CAATTTCAAT GGGTCCAAAT GAATATTCAC TGGAAGAATC ATCGTTTTGA ACTACTGGTT GAGTATTCCA GAATTTGTGT GGTTTGATCA TCGAACCAGG ACCCTGGGTC TGAGCTTCCA GGGTACCCAT
MAHHHHHHMG TLEAQTQGPG SIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADARIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT CAAACCACAC AAATTCTGGA ATACTCAACC AGTAGTTCAA AACGATGATT CTTCCAGTGA ATATTCATTT GGACCCATTG AAATTGAACC AGATTCTTTT AGAAAAGAAA TATATAAGCT ACCAGATGGA TTCTCTTGGT TTGATTGTAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATGCA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AAAAGAGCTT GGAAAACAAG CATATATGAA GCTGGATAAG ATTGAAGATT TGCAAGACAA AATCACAATT CATCAATGCT TTAATGTTGA AGATGTTAAA CATTATTTCA CGAATATTGA TAAAGTTATT GTAACTTATG TTCGAGAAAA TAAAAATAAA GAAATTACTG ACTTGTTTAG CTTCTTTATA ATAGAATCCA CTGTCATCAA TAATGAAAGA TTTCCAACTA TTAATATTGC ATATTCATAC TTCAATATTG CAAATACCTG CTCATTAAAA GAATTGTTCA ATGAAATGCT TATTACTGCC AAAAATAATA ATTGTGATGC TTTTAATACA TTGGATCTGA TGCAAAATTT ACAAGTTATT CAAGATAGTA AATTTATTAT CGGTACCGGT AGATTAAGAT ATTATGTGTT TAATTGGAAG ATACCTCAAA TTTCACCTTC AAATGTTGGA ATTATATTAT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
harvested on: 7/27/2017
sequenced on: 7/27/2017
expected MW: 53kDa
observed MW: 53kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Many (50-100)
total expression level:
High Expression
soluble expression level:
Moderate Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 99
percent coverage: 95
MIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSAALGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
ATTAAAATAA TATAATTCCA ACATTTGAAG GTGAAATTTG AGGTATCTTC CAATTAAACA CATAATATCT TAATCTACCG GTACCGATAA TAAATTTACT ATCTTGAATA ACTTGTAAAT TTTGCATCAG ATCCAATGTA TTAAAAGCAT CACAATTATT ATTTTTGGCA GTAATAAGCA TTTCATTGAA CAATTCTTTT AATGAGCAGG TATTTGCAAT ATTGAAGTAT GAATATGCAA TATTAATAGT TGGAAATCTT TCATTATTGA TGACAGTGGA TTCTATTATA AAGAAGCTAA ACAAGTCAGT AATTTCTTTA TTTTTATTTT CTCGAACATA AGTTACAATA ACTTTATCAA TATTCGTGAA ATAATGTTTA ACATCTTCAA CATTAAAGCA TTGATGAATT GTGATTTTGT CTTGCAAATC TTCAATCTTA TCCAGCTTCA TATATGCTTG TTTTCCAAGC GCTGCTGAAT GGTTAATTTC ATCATAGTTT ATCAGATTAT TCATGGTTTC GTTGCTGACA TCTTTATATT TCATAAAGTA ATTTTCGAAT AATTTACAGA TTTGTTCTGC ATCAGAATCT ACGGAGGGTC TAAACCCTTC AATTCTTTTA TCAGCAGGAA TACGATGATA TTTAATTAAA GAGCTCATGG TCATATTTCT TGGGATTCCA ATAAAACCAG CTTCTAATAA TTTCTTTACA TTAATTATAC GATGCCAATA AGTTCCAATA GTAAAAGGTT TGGTAATATT CTTCCCACAA GTGTAAATTG ATTGAAAGAT TTTTTCACAT CTGATTCTTC TTGTAATTTC CTTAATAAGT ACAGGGGCAA GTCTTTTAGA TCTAAGCTTC TTATGAACGC AAAGAAAATT AACTACAGAA GTATTCATAA TGCAATTATG AATTCTAACT TTGATTGGGA TTGCGGAAAT AAATCCAACC ATTTTCTTTG TTTCATTAAC TCTGACTCCA ACAAGCCAGT TCTTTTTTTG ACCAGGGACA CAAAGAGCCC ATCTAAGAAA CTCCTTTGAA TAATTAAATC TAAATTGGGA ATCGTCATCT TCAACATAAT GGTCTTTTAA TAATTGGTAA GTATCTTCAA AATCTTGAGA TTCTATATCC CATAAATTAC AATCAAACCA AGAGAATCCA TCTGGTAGCT TATATATTTC TTTTCTAAAA GAATCTGGTT CAATTTCAAT GGGTCCAAAT GAATATTCAC TGGAAGAATC ATCGTTTTGA ACTACTGGTT GAGTATTCCA GAATTTGTGT GGTTTGATCA TCGAACCAGG ACCCTGGGTC TGAGCTTCCA GGGT
MAHHHHHHMG TLEAQTQGPG SIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSAALGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT CAAACCACAC AAATTCTGGA ATACTCAACC AGTAGTTCAA AACGATGATT CTTCCAGTGA ATATTCATTT GGACCCATTG AAATTGAACC AGATTCTTTT AGAAAAGAAA TATATAAGCT ACCAGATGGA TTCTCTTGGT TTGATTGTAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATAAA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AGCAGCGCTT GGAAAACAAG CATATATGAA GCTGGATAAG ATTGAAGATT TGCAAGACAA AATCACAATT CATCAATGCT TTAATGTTGA AGATGTTAAA CATTATTTCA CGAATATTGA TAAAGTTATT GTAACTTATG TTCGAGAAAA TAAAAATAAA GAAATTACTG ACTTGTTTAG CTTCTTTATA ATAGAATCCA CTGTCATCAA TAATGAAAGA TTTCCAACTA TTAATATTGC ATATTCATAC TTCAATATTG CAAATACCTG CTCATTAAAA GAATTGTTCA ATGAAATGCT TATTACTGCC AAAAATAATA ATTGTGATGC TTTTAATACA TTGGATCTGA TGCAAAATTT ACAAGTTATT CAAGATAGTA AATTTATTAT CGGTACCGGT AGATTAAGAT ATTATGTGTT TAATTGGAAG ATACCTCAAA TTTCACCTTC AAATGTTGGA ATTATATTAT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
harvested on: 10/23/2015
sequenced on: 10/23/2015
expected MW: 47kDa
observed MW: 47kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Too many to count (100+)
total expression level:
High Expression
soluble expression level:
No Expression
expression host:
data unavailable
sequencing result:
percent identity: data unavailable
percent coverage: data unavailable
MAHHHHHHMG TLEAQTQGPG SNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATAAA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AAAAGAGCTT GGAAAACAAG CATATATGAA GCTGGATAAG ATTGAAGATT TGCAAGACAA AATCACAATT CATCAATGCT TTAATGTTGA AGATGTTAAA CATTATTTCA CGAATATTGA TAAAGTTATT GTAACTTATG TTCGAGAAAA TAAAAATAAA GAAATTACTG ACTTGTTTAG CTTCTTTATA ATAGAATCCA CTGTCATCAA TAATGAAAGA TTTCCAACTA TTAATATTGC ATATTCATAC TTCAATATTG CAAATACCTG CTCATTAAAA GAATTGTTCA ATGAAATGCT TATTACTGCC AAAAATAATA ATTGTGATGC TTTTAATACA TTGGATCTGA TGCAAAATTT ACAAGTTATT CAAGATAGTA AATTTATTAT CGGTACCGGT AGATTAAGAT ATTATGTGTT TAATTGGAAG ATACCTCAAA TTTCACCTTC AAATGTTGGA ATTATATTAT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
harvested on: 10/23/2015
sequenced on: 10/23/2015
expected MW: 48kDa
observed MW: 48kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Too many to count (100+)
total expression level:
Moderate Expression
soluble expression level:
Moderate Expression
expression host:
data unavailable
sequencing result:
percent identity: 100
percent coverage: 99
MAHHHHHHMG TLEAQTQGPG SMIYKLPDGF SWFDCNLWDI ESQDFEDTYQ LLKDHYVEDD DSQFRFNYSK EFLRWALCVP GQKKNWLVGV RVNETKKMVG FISAIPIKVR IHNCIMNTSV VNFLCVHKKL RSKRLAPVLI KEITRRIRCE KIFQSIYTCG KNITKPFTIG TYWHRIINVK KLLEAGFIGI PRNMTMSSLI KYHRIPADKR IEGFRPSVDS DAEQICKLFE NYFMKYKDVS NETMNNLINY DEINHSKELG KQAYMKLDKI EDLQDKITIH QCFNVEDVKH YFTNIDKVIV TYVRENKNKE ITDLFSFFII ESTVINNERF PTINIAYSYF NIANTCSLKE LFNEMLITAK NNNCDAFNTL DLMQNLQVIQ DSKFIIGTGR LRYYVFNWKI PQISPSNVGI I
TAATATAATT CCAACATTTG AAGGTGAAAT TTGAGGTATC TTCCAATTAA ACACATAATA TCTTAATCTA CCGGTACCGA TAATAAATTT ACTATCTTGA ATAACTTGTA AATTTTGCAT CAGATCCAAT GTATTAAAAG CATCACAATT ATTATTTTTG GCAGTAATAA GCATTTCATT GAACAATTCT TTTAATGAGC AGGTATTTGC AATATTGAAG TATGAATATG CAATATTAAT AGTTGGAAAT CTTTCATTAT TGATGACAGT GGATTCTATT ATAAAGAAGC TAAACAAGTC AGTAATTTCT TTATTTTTAT TTTCTCGAAC ATAAGTTACA ATAACTTTAT CAATATTCGT GAAATAATGT TTAACATCTT CAACATTAAA GCATTGATGA ATTGTGATTT TGTCTTGCAA ATCTTCAATC TTATCCAGCT TCATATATGC TTGTTTTCCA AGCTCTTTTG AATGGTTAAT TTCATCATAG TTTATCAGAT TATTCATGGT TTCGTTGCTG ACATCTTTAT ATTTCATAAA GTAATTTTCG AATAATTTAC AGATTTGTTC TGCATCAGAA TCTACGGAGG GTCTAAACCC TTCAATTCTT TTATCAGCAG GAATACGATG ATATTTAATT AAAGAGCTCA TGGTCATATT TCTTGGGATT CCAATAAAAC CAGCTTCTAA TAATTTCTTT ACATTAATTA TACGATGCCA ATAAGTTCCA ATAGTAAAAG GTTTGGTAAT ATTCTTCCCA CAAGTGTAAA TTGATTGAAA GATTTTTTCA CATCTGATTC TTCTTGTAAT TTCCTTAATA AGTACAGGGG CAAGTCTTTT AGATCTAAGC TTCTTATGAA CGCAAAGAAA ATTAACTACA GAAGTATTCA TAATGCAATT ATGAATTCTA ACTTTGATTG GGATTGCGGA AATAAATCCA ACCATTTTCT TTGTTTCATT AACTCTGACT CCAACAAGCC AGTTCTTTTT TTGACCAGGG ACACAAAGAG CCCATCTAAG AAACTCCTTT GAATAATTAA ATCTAAATTG GGAATCGTCA TCTTCAACAT AATGGTCTTT TAATAATTGG TAAGTATCTT CAAAATCTTG AGATTCTATA TCCCATAAAT TACAATCAAA CCAAGAGAAT CCATCTGGTA GCTTATATAT CATCGAACCA GGACCCTGGG TCTGAGCTTC CAGGGTACCC ATATGATGGT GATGGTGATG AGCCAT
MAHHHHHHMG TLEAQTQGPG SIYKLPDGFS WFDCNLWDIE SQDFEDTYQL LKDHYVEDDD SQFRFNYSKE FLRWALCVPG QKKNWLVGVR VNETKKMVGF ISAIPIKVRI HNCIMNTSVV NFLCVHKKLR SKRLAPVLIK EITRRIRCEK IFQSIYTCGK NITKPFTIGT YWHRIINVKK LLEAGFIGIP RNMTMSSLIK YHRIPADKRI EGFRPSVDSD AEQICKLFEN YFMKYKDVSN ETMNNLINYD EINHSKELGK QAYMKLDKIE DLQDKITIHQ CFNVEDVKHY FTNIDKVIVT YVRENKNKEI TDLFSFFIIE STVINNERFP TINIAYSYFN IANTCSLKEL FNEMLITAKN NNCDAFNTLD LMQNLQVIQD SKFIIGTGRL RYYVFNWKIP QISPSNVGII LF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT ATATAAGCTA CCAGATGGAT TCTCTTGGTT TGATTGTAAT TTATGGGATA TAGAATCTCA AGATTTTGAA GATACTTACC AATTATTAAA AGACCATTAT GTTGAAGATG ACGATTCCCA ATTTAGATTT AATTATTCAA AGGAGTTTCT TAGATGGGCT CTTTGTGTCC CTGGTCAAAA AAAGAACTGG CTTGTTGGAG TCAGAGTTAA TGAAACAAAG AAAATGGTTG GATTTATTTC CGCAATCCCA ATCAAAGTTA GAATTCATAA TTGCATTATG AATACTTCTG TAGTTAATTT TCTTTGCGTT CATAAGAAGC TTAGATCTAA AAGACTTGCC CCTGTACTTA TTAAGGAAAT TACAAGAAGA ATCAGATGTG AAAAAATCTT TCAATCAATT TACACTTGTG GGAAGAATAT TACCAAACCT TTTACTATTG GAACTTATTG GCATCGTATA ATTAATGTAA AGAAATTATT AGAAGCTGGT TTTATTGGAA TCCCAAGAAA TATGACCATG AGCTCTTTAA TTAAATATCA TCGTATTCCT GCTGATAAAA GAATTGAAGG GTTTAGACCC TCCGTAGATT CTGATGCAGA ACAAATCTGT AAATTATTCG AAAATTACTT TATGAAATAT AAAGATGTCA GCAACGAAAC CATGAATAAT CTGATAAACT ATGATGAAAT TAACCATTCA AAAGAGCTTG GAAAACAAGC ATATATGAAG CTGGATAAGA TTGAAGATTT GCAAGACAAA ATCACAATTC ATCAATGCTT TAATGTTGAA GATGTTAAAC ATTATTTCAC GAATATTGAT AAAGTTATTG TAACTTATGT TCGAGAAAAT AAAAATAAAG AAATTACTGA CTTGTTTAGC TTCTTTATAA TAGAATCCAC TGTCATCAAT AATGAAAGAT TTCCAACTAT TAATATTGCA TATTCATACT TCAATATTGC AAATACCTGC TCATTAAAAG AATTGTTCAA TGAAATGCTT ATTACTGCCA AAAATAATAA TTGTGATGCT TTTAATACAT TGGATCTGAT GCAAAATTTA CAAGTTATTC AAGATAGTAA ATTTATTATC GGTACCGGTA GATTAAGATA TTATGTGTTT AATTGGAAGA TACCTCAAAT TTCACCTTCA AATGTTGGAA TTATATTATT TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
harvested on: 12/14/2017
sequenced on: 12/6/2017
expected MW: 53kDa
observed MW: 50kDa
MW ratio: 0.94
antibiotic marker:
ampicillin
expression colonies count:
Good (10-50)
total expression level:
Moderate Expression
soluble expression level:
Low Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: data unavailable
percent coverage: data unavailable
MAHHHHHHMG TLEAQTQGPG SNAIKPHKFW NTQPVVQNDD SSSEYSFGPI EIEPDSFRKE IYKLPDGFSW FDCNLWDIES QDFEDTYQLL KDHYVEDDDS QFRFNYSKEF LRWALCVPGQ KKNWLVGVRV NETKKMVGFI SAIPIKVRIH NCIMNTSVVN FLCVHKKLRS KRLAPVLIKE ITRRIRCEKI FQSIYTCGKN ITKPFTIGTY WHRIINVKKL LEAGFIGIPR NMTMSSLIKY HRIPADKRIE GFRPSVDSDA EQICKLFENY FMKYKDVSNE TMNNLINYDE INHSKELGKQ AYMKLDKIED LQDKITIHQC FNVEDVKHYF TNIDKVIVTY VRENKNKEIT DLFSFFIIES TVINNERFPT INIAYSYFNI ANTCSLKELF NEMLITAKNN NCDAFNTLDL MQNLQVIQDS KFIIGTGRLR YYVFNWKIPQ ISPSNVGIIL F
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAA TGCAATCAAA CCACACAAAT TCTGGAATAC TCAACCAGTA GTTCAAAACG ATGATTCTTC CAGTGAATAT TCATTTGGAC CCATTGAAAT TGAACCAGAT TCTTTTAGAA AAGAAATATA TAAGCTACCA GATGGATTCT CTTGGTTTGA TTGTAATTTA TGGGATATAG AATCTCAAGA TTTTGAAGAT ACTTACCAAT TATTAAAAGA CCATTATGTT GAAGATGACG ATTCCCAATT TAGATTTAAT TATTCAAAGG AGTTTCTTAG ATGGGCTCTT TGTGTCCCTG GTCAAAAAAA GAACTGGCTT GTTGGAGTCA GAGTTAATGA AACAAAGAAA ATGGTTGGAT TTATTTCCGC AATCCCAATC AAAGTTAGAA TTCATAATTG CATTATGAAT ACTTCTGTAG TTAATTTTCT TTGCGTTCAT AAGAAGCTTA GATCTAAAAG ACTTGCCCCT GTACTTATTA AGGAAATTAC AAGAAGAATC AGATGTGAAA AAATCTTTCA ATCAATTTAC ACTTGTGGGA AGAATATTAC CAAACCTTTT ACTATTGGAA CTTATTGGCA TCGTATAATT AATGTAAAGA AATTATTAGA AGCTGGTTTT ATTGGAATCC CAAGAAATAT GACCATGAGC TCTTTAATTA AATATCATCG TATTCCTGCT GATAAAAGAA TTGAAGGGTT TAGACCCTCC GTAGATTCTG ATGCAGAACA AATCTGTAAA TTATTCGAAA ATTACTTTAT GAAATATAAA GATGTCAGCA ACGAAACCAT GAATAATCTG ATAAACTATG ATGAAATTAA CCATTCAAAA GAGCTTGGAA AACAAGCATA TATGAAGCTG GATAAGATTG AAGATTTGCA AGACAAAATC ACAATTCATC AATGCTTTAA TGTTGAAGAT GTTAAACATT ATTTCACGAA TATTGATAAA GTTATTGTAA CTTATGTTCG AGAAAATAAA AATAAAGAAA TTACTGACTT GTTTAGCTTC TTTATAATAG AATCCACTGT CATCAATAAT GAAAGATTTC CAACTATTAA TATTGCATAT TCATACTTCA ATATTGCAAA TACCTGCTCA TTAAAAGAAT TGTTCAATGA AATGCTTATT ACTGCCAAAA ATAATAATTG TGATGCTTTT AATACATTGG ATCTGATGCA AAATTTACAA GTTATTCAAG ATAGTAAATT TATTATCGGT ACCGGTAGAT TAAGATATTA TGTGTTTAAT TGGAAGATAC CTCAAATTTC ACCTTCAAAT GTTGGAATTA TATTATTTTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
harvested on: 1/8/2018
sequenced on: 12/12/2017
expected MW: 53kDa
observed MW: 52kDa
MW ratio: 0.98
antibiotic marker:
ampicillin
expression colonies count:
Good (10-50)
total expression level:
High Expression
soluble expression level:
Low Expression
expression host:
BL 21 (DE3) Rosetta
sequencing result:
percent identity: 89
percent coverage: 72
MGTLEAQTQG PGSAIKPHKF WNTQPVVQND DSSSEYSFGP IEIEPDSFRK EIYKLPDGFS WFDCNLWDIE SQDFEDTYQL LKDHYVEDDD SQFRFNYSKE FLRWALCVPG QKKNWLVGVR VNETKKMVGF ISAIPIKVRI HNCIMNTSVV NFLCVHKKLR SKRLAPVLIK EITRRIRCEK IFQSIYTCGK NITKPFTIGT YWHRIINVKK LLEAGFIGIP RNMTMSSLIK YHRIPADKRI EGFRPSVDSD AEQICKLFEN YFXXYKDVSN ETMNNLINYD EXXHSKELXK QAYMKXXXXX XXXXXXXXHQ CXXXXXXXXX XXXXXX
GGNCTCATCA CCATCACCAT CATATGGGTA CCCTGGAAGC TCAGACCCAG GGTCCTGGTT CGGCAATCAA ACCACACAAA TTCTGGAATA CTCAACCAGT AGTTCAAAAC GATGATTCTT CCAGTGAATA TTCATTTGGA CCCATTGAAA TTGAACCAGA TTCTTTTAGA AAAGAAATAT ATAAGCTACC AGATGGATTC TCTTGGTTTG ATTGTAATTT ATGGGATATA GAATCTCAAG ATTTTGAAGA TACTTACCAA TTATTAAAAG ACCATTATGT TGAAGATGAC GATTCCCAAT TTAGATTTAA TTATTCAAAG GAGTTTCTTA GATGGGCTCT TTGTGTCCCT GGTCAAAAAA AGAACTGGCT TGTTGGAGTC AGAGTTAATG AAACAAAGAA AATGGTTGGA TTTATTTCCG CAATCCCAAT CAAAGTTAGA ATTCATAATT GCATTATGAA TACTTCTGTA GTTAATTTTC TTTGCGTTCA TAAGAAGCTT AGATCTAAAA GACTTGCCCC TGTACTTATT AAGGAAATTA CAAGAAGAAT CAGATGTGAA AAAATCTTTC AATCAATTTA CACTTGTGGG AAGAATATTA CCAAACCTTT TACTATTGGA ACTTATTGGC ATCGTATAAT TAATGTAAAG AAATTATTAG AAGCTGGTTT TATTGGAATC CCAAGAAATA TGACCATGAG CTCTTTAATT AAATATCATC GTATTCCTGC TGATAAAAGA ATTGAAGGGT TTAGACCCTC CGTAGATTCT GATGCAGAAC AAATCTGTAA ATTATTCGAA AATTACTTTA TNNNNTATAA AGATGTCAGC AACGAAACCA TGAATAATCT GATAAACTAT GATGAAANNN ACCATTCAAA AGAGCTTNNN AAACAAGCAT ATATGAAGNN NGNNNNNNNN NNNNNNNNNC NNNNNNNNNT NNNNNNTCAT CAATGCTNNN NNNNNNNNNN NNNNNNNCNN NNNNNNNNNN NNNNTNNNNN NG
MAHHHHHHMG TLEAQTQGPG SAIKPHKFWN TQPVVQNDDS SSEYSFGPIE IEPDSFRKEI YKLPDGFSWF DCNLWDIESQ DFEDTYQLLK DHYVEDDDSQ FRFNYSKEFL RWALCVPGQK KNWLVGVRVN ETKKMVGFIS AIPIKVRIHN CIMNTSVVNF LCVHKKLRSK RLAPVLIKEI TRRIRCEKIF QSIYTCGKNI TKPFTIGTYW HRIINVKKLL EAGFIGIPRN MTMSSLIKYH RIPADKRIEG FRPSVDSDAE QICKLFENYF MKYKDVSNET MNNLINYDEI NHSKELGKQA YMKLDKIEDL QDKITIHQCF NVEDVKHYFT NIDKVIVTYV RENKNKEITD LFSFFIIEST VINNERFPTI NIAYSYFNIA NTCSLKELFN EMLITAKNNN CDAFNTLDLM QNLQVIQDSK FIIGTGRLRY YVFNWKIPQI SPSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGC AATCAAACCA CACAAATTCT GGAATACTCA ACCAGTAGTT CAAAACGATG ATTCTTCCAG TGAATATTCA TTTGGACCCA TTGAAATTGA ACCAGATTCT TTTAGAAAAG AAATATATAA GCTACCAGAT GGATTCTCTT GGTTTGATTG TAATTTATGG GATATAGAAT CTCAAGATTT TGAAGATACT TACCAATTAT TAAAAGACCA TTATGTTGAA GATGACGATT CCCAATTTAG ATTTAATTAT TCAAAGGAGT TTCTTAGATG GGCTCTTTGT GTCCCTGGTC AAAAAAAGAA CTGGCTTGTT GGAGTCAGAG TTAATGAAAC AAAGAAAATG GTTGGATTTA TTTCCGCAAT CCCAATCAAA GTTAGAATTC ATAATTGCAT TATGAATACT TCTGTAGTTA ATTTTCTTTG CGTTCATAAG AAGCTTAGAT CTAAAAGACT TGCCCCTGTA CTTATTAAGG AAATTACAAG AAGAATCAGA TGTGAAAAAA TCTTTCAATC AATTTACACT TGTGGGAAGA ATATTACCAA ACCTTTTACT ATTGGAACTT ATTGGCATCG TATAATTAAT GTAAAGAAAT TATTAGAAGC TGGTTTTATT GGAATCCCAA GAAATATGAC CATGAGCTCT TTAATTAAAT ATCATCGTAT TCCTGCTGAT AAAAGAATTG AAGGGTTTAG ACCCTCCGTA GATTCTGATG CAGAACAAAT CTGTAAATTA TTCGAAAATT ACTTTATGAA ATATAAAGAT GTCAGCAACG AAACCATGAA TAATCTGATA AACTATGATG AAATTAACCA TTCAAAAGAG CTTGGAAAAC AAGCATATAT GAAGCTGGAT AAGATTGAAG ATTTGCAAGA CAAAATCACA ATTCATCAAT GCTTTAATGT TGAAGATGTT AAACATTATT TCACGAATAT TGATAAAGTT ATTGTAACTT ATGTTCGAGA AAATAAAAAT AAAGAAATTA CTGACTTGTT TAGCTTCTTT ATAATAGAAT CCACTGTCAT CAATAATGAA AGATTTCCAA CTATTAATAT TGCATATTCA TACTTCAATA TTGCAAATAC CTGCTCATTA AAAGAATTGT TCAATGAAAT GCTTATTACT GCCAAAAATA ATAATTGTGA TGCTTTTAAT ACATTGGATC TGATGCAAAA TTTACAAGTT ATTCAAGATA GTAAATTTAT TATCGGTACC GGTAGATTAA GATATTATGT GTTTAATTGG AAGATACCTC AAATTTCACC TTCAAATGTT GGAATTATAT TATTTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
harvested on: 6/26/2012
sequenced on: 1/25/2017
expected MW: 56kDa
observed MW: 56kDa
MW ratio: 1
antibiotic marker:
ampicillin
expression colonies count:
Too many to count (100+)
total expression level:
Moderate Expression
soluble expression level:
Low Expression
expression host:
data unavailable
sequencing result:
pass
percent identity: 100
percent coverage: 100
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TTACTCACTT AAAATAATAT AATTCCAACA TTTGAAGGTG AAATTTGAGG TATCTTCCAA TTAAACACAT AATATCTTAA TCTACCGGTA CCGATAATAA ATTTACTATC TTGAATAACT TGTAAATTTT GCATCAGATC CAATGTATTA AAAGCATCAC AATTATTATT TTTGGCAGTA ATAAGCATTT CATTGAACAA TTCTTTTAAT GAGCAGGTAT TTGCAATATT GAAGTATGAA TATGCAATAT TAATAGTTGG AAATCTTTCA TTATTGATGA CAGTGGATTC TATTATAAAG AAGCTAAACA AGTCAGTAAT TTCTTTATTT TTATTTTCTC GAACATAAGT TACAATAACT TTATCAATAT TCGTGAAATA ATGTTTAACA TCTTCAACAT TAAAGCATTG ATGAATTGTG ATTTTGTCTT GCAAATCTTC AATCTTATCC AGCTTCATAT ATGCTTGTTT TCCAAGCTCT TTTGAATGGT TAATTTCATC ATAGTTTATC AGATTATTCA TGGTTTCGTT GCTGACATCT TTATATTTCA TAAAGTAATT TTCGAATAAT TTACAGATTT GTTCTGCATC AGAATCTACG GAGGGTCTAA ACCCTTCAAT TCTTTTATCA GCAGGAATAC GATGATATTT AATTAAAGAG CTCATGGTCA TATTTCTTGG GATTCCAATA AAACCAGCTT CTAATAATTT CTTTACATTA ATTATACGAT GCCAATAAGT TCCAATAGTA AAAGGTTTGG TAATATTCTT CCCACAAGTG TAAATTGATT GAAAGATTTT TTCACATCTG ATTCTTCTTG TAATTTCCTT AATAAGTACA GGGGCAAGTC TTTTAGATCT AAGCTTCTTA TGAACGCAAA GAAAATTAAC TACAGAAGTA TTCATAATGC AATTATGAAT TCTAACTTTG ATTGGGATTG CGGAAATAAA TCCAACCATT TTCTTTGTTT CATTAACTCT GACTCCAACA AGCCAGTTCT TTTTTTGACC AGGGACACAA AGAGCCCATC TAAGAAACTC CTTTGAATAA TTAAATCTAA ATTGGGAATC GTCATCTTCA ACATAATGGT CTTTTAATAA TTGGTAAGTA TCTTCAAAAT CTTGAGATTC TATATCCCAT AAATTACAAT CAAACCAAGA GAATCCATCT GGTAGCTTAT ATATTTCTTT TCTAAAAGAA TCTGGTTCAA TTTCAATGGG TCCAAATGAA TATTCACTGG AAGAATCATC GTTTTGAACT ACTGGTTGAG TATTCCAGAA TTTGTGTGGT TTGATTGCAT TTGCGGCAGT GTTCATAAAG CTACCTAAGC TCATTTCTTT CAGAAGATTT GTGATCTTCT TGGTATTTGA AATGGAAGAT TCTGAATTCT CTGGGGTTTC ACCACTCATT CTAATAAGCA TATGGTGGTG GTGGTGGTGA GCCAT
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTA TTAGAATGAG TGGTGAAACC CCAGAGAATT CAGAATCTTC CATTTCAAAT ACCAAGAAGA TCACAAATCT TCTGAAAGAA ATGAGCTTAG GTAGCTTTAT GAACACTGCC GCAAATGCAA TCAAACCACA CAAATTCTGG AATACTCAAC CAGTAGTTCA AAACGATGAT TCTTCCAGTG AATATTCATT TGGACCCATT GAAATTGAAC CAGATTCTTT TAGAAAAGAA ATATATAAGC TACCAGATGG ATTCTCTTGG TTTGATTGTA ATTTATGGGA TATAGAATCT CAAGATTTTG AAGATACTTA CCAATTATTA AAAGACCATT ATGTTGAAGA TGACGATTCC CAATTTAGAT TTAATTATTC AAAGGAGTTT CTTAGATGGG CTCTTTGTGT CCCTGGTCAA AAAAAGAACT GGCTTGTTGG AGTCAGAGTT AATGAAACAA AGAAAATGGT TGGATTTATT TCCGCAATCC CAATCAAAGT TAGAATTCAT AATTGCATTA TGAATACTTC TGTAGTTAAT TTTCTTTGCG TTCATAAGAA GCTTAGATCT AAAAGACTTG CCCCTGTACT TATTAAGGAA ATTACAAGAA GAATCAGATG TGAAAAAATC TTTCAATCAA TTTACACTTG TGGGAAGAAT ATTACCAAAC CTTTTACTAT TGGAACTTAT TGGCATCGTA TAATTAATGT AAAGAAATTA TTAGAAGCTG GTTTTATTGG AATCCCAAGA AATATGACCA TGAGCTCTTT AATTAAATAT CATCGTATTC CTGCTGATAA AAGAATTGAA GGGTTTAGAC CCTCCGTAGA TTCTGATGCA GAACAAATCT GTAAATTATT CGAAAATTAC TTTATGAAAT ATAAAGATGT CAGCAACGAA ACCATGAATA ATCTGATAAA CTATGATGAA ATTAACCATT CAAAAGAGCT TGGAAAACAA GCATATATGA AGCTGGATAA GATTGAAGAT TTGCAAGACA AAATCACAAT TCATCAATGC TTTAATGTTG AAGATGTTAA ACATTATTTC ACGAATATTG ATAAAGTTAT TGTAACTTAT GTTCGAGAAA ATAAAAATAA AGAAATTACT GACTTGTTTA GCTTCTTTAT AATAGAATCC ACTGTCATCA ATAATGAAAG ATTTCCAACT ATTAATATTG CATATTCATA CTTCAATATT GCAAATACCT GCTCATTAAA AGAATTGTTC AATGAAATGC TTATTACTGC CAAAAATAAT AATTGTGATG CTTTTAATAC ATTGGATCTG ATGCAAAATT TACAAGTTAT TCAAGATAGT AAATTTATTA TCGGTACCGG TAGATTAAGA TATTATGTGT TTAATTGGAA GATACCTCAA ATTTCACCTT CAAATGTTGG AATTATATTA TTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
purified on: 2/7/2017
concentration: 23.1mg/ml
observed molecular weight:
60kDa
expression level:
Low Expression
protein purification buffer:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
expression host:
data unavailable
vial count (approx.):
128
vial volume:
6µl
percent identity: 100
percent coverage: 100
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TTACTCACTT AAAATAATAT AATTCCAACA TTTGAAGGTG AAATTTGAGG TATCTTCCAA TTAAACACAT AATATCTTAA TCTACCGGTA CCGATAATAA ATTTACTATC TTGAATAACT TGTAAATTTT GCATCAGATC CAATGTATTA AAAGCATCAC AATTATTATT TTTGGCAGTA ATAAGCATTT CATTGAACAA TTCTTTTAAT GAGCAGGTAT TTGCAATATT GAAGTATGAA TATGCAATAT TAATAGTTGG AAATCTTTCA TTATTGATGA CAGTGGATTC TATTATAAAG AAGCTAAACA AGTCAGTAAT TTCTTTATTT TTATTTTCTC GAACATAAGT TACAATAACT TTATCAATAT TCGTGAAATA ATGTTTAACA TCTTCAACAT TAAAGCATTG ATGAATTGTG ATTTTGTCTT GCAAATCTTC AATCTTATCC AGCTTCATAT ATGCTTGTTT TCCAAGCTCT TTTGAATGGT TAATTTCATC ATAGTTTATC AGATTATTCA TGGTTTCGTT GCTGACATCT TTATATTTCA TAAAGTAATT TTCGAATAAT TTACAGATTT GTTCTGCATC AGAATCTACG GAGGGTCTAA ACCCTTCAAT TCTTTTATCA GCAGGAATAC GATGATATTT AATTAAAGAG CTCATGGTCA TATTTCTTGG GATTCCAATA AAACCAGCTT CTAATAATTT CTTTACATTA ATTATACGAT GCCAATAAGT TCCAATAGTA AAAGGTTTGG TAATATTCTT CCCACAAGTG TAAATTGATT GAAAGATTTT TTCACATCTG ATTCTTCTTG TAATTTCCTT AATAAGTACA GGGGCAAGTC TTTTAGATCT AAGCTTCTTA TGAACGCAAA GAAAATTAAC TACAGAAGTA TTCATAATGC AATTATGAAT TCTAACTTTG ATTGGGATTG CGGAAATAAA TCCAACCATT TTCTTTGTTT CATTAACTCT GACTCCAACA AGCCAGTTCT TTTTTTGACC AGGGACACAA AGAGCCCATC TAAGAAACTC CTTTGAATAA TTAAATCTAA ATTGGGAATC GTCATCTTCA ACATAATGGT CTTTTAATAA TTGGTAAGTA TCTTCAAAAT CTTGAGATTC TATATCCCAT AAATTACAAT CAAACCAAGA GAATCCATCT GGTAGCTTAT ATATTTCTTT TCTAAAAGAA TCTGGTTCAA TTTCAATGGG TCCAAATGAA TATTCACTGG AAGAATCATC GTTTTGAACT ACTGGTTGAG TATTCCAGAA TTTGTGTGGT TTGATTGCAT TTGCGGCAGT GTTCATAAAG CTACCTAAGC TCATTTCTTT CAGAAGATTT GTGATCTTCT TGGTATTTGA AATGGAAGAT TCTGAATTCT CTGGGGTTTC ACCACTCATT CTAATAAGCA TATGGTGGTG GTGGTGGTGA GCCAT
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTA TTAGAATGAG TGGTGAAACC CCAGAGAATT CAGAATCTTC CATTTCAAAT ACCAAGAAGA TCACAAATCT TCTGAAAGAA ATGAGCTTAG GTAGCTTTAT GAACACTGCC GCAAATGCAA TCAAACCACA CAAATTCTGG AATACTCAAC CAGTAGTTCA AAACGATGAT TCTTCCAGTG AATATTCATT TGGACCCATT GAAATTGAAC CAGATTCTTT TAGAAAAGAA ATATATAAGC TACCAGATGG ATTCTCTTGG TTTGATTGTA ATTTATGGGA TATAGAATCT CAAGATTTTG AAGATACTTA CCAATTATTA AAAGACCATT ATGTTGAAGA TGACGATTCC CAATTTAGAT TTAATTATTC AAAGGAGTTT CTTAGATGGG CTCTTTGTGT CCCTGGTCAA AAAAAGAACT GGCTTGTTGG AGTCAGAGTT AATGAAACAA AGAAAATGGT TGGATTTATT TCCGCAATCC CAATCAAAGT TAGAATTCAT AATTGCATTA TGAATACTTC TGTAGTTAAT TTTCTTTGCG TTCATAAGAA GCTTAGATCT AAAAGACTTG CCCCTGTACT TATTAAGGAA ATTACAAGAA GAATCAGATG TGAAAAAATC TTTCAATCAA TTTACACTTG TGGGAAGAAT ATTACCAAAC CTTTTACTAT TGGAACTTAT TGGCATCGTA TAATTAATGT AAAGAAATTA TTAGAAGCTG GTTTTATTGG AATCCCAAGA AATATGACCA TGAGCTCTTT AATTAAATAT CATCGTATTC CTGCTGATAA AAGAATTGAA GGGTTTAGAC CCTCCGTAGA TTCTGATGCA GAACAAATCT GTAAATTATT CGAAAATTAC TTTATGAAAT ATAAAGATGT CAGCAACGAA ACCATGAATA ATCTGATAAA CTATGATGAA ATTAACCATT CAAAAGAGCT TGGAAAACAA GCATATATGA AGCTGGATAA GATTGAAGAT TTGCAAGACA AAATCACAAT TCATCAATGC TTTAATGTTG AAGATGTTAA ACATTATTTC ACGAATATTG ATAAAGTTAT TGTAACTTAT GTTCGAGAAA ATAAAAATAA AGAAATTACT GACTTGTTTA GCTTCTTTAT AATAGAATCC ACTGTCATCA ATAATGAAAG ATTTCCAACT ATTAATATTG CATATTCATA CTTCAATATT GCAAATACCT GCTCATTAAA AGAATTGTTC AATGAAATGC TTATTACTGC CAAAAATAAT AATTGTGATG CTTTTAATAC ATTGGATCTG ATGCAAAATT TACAAGTTAT TCAAGATAGT AAATTTATTA TCGGTACCGG TAGATTAAGA TATTATGTGT TTAATTGGAA GATACCTCAA ATTTCACCTT CAAATGTTGG AATTATATTA TTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
purified on: 5/28/2013
concentration: 17.5mg/ml
observed molecular weight:
48kDa
expression level:
Low Expression
protein purification buffer:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
expression host:
data unavailable
vial count (approx.):
1
vial volume:
110µl
percent identity: 100
percent coverage: 100
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TTACTCACTT AAAATAATAT AATTCCAACA TTTGAAGGTG AAATTTGAGG TATCTTCCAA TTAAACACAT AATATCTTAA TCTACCGGTA CCGATAATAA ATTTACTATC TTGAATAACT TGTAAATTTT GCATCAGATC CAATGTATTA AAAGCATCAC AATTATTATT TTTGGCAGTA ATAAGCATTT CATTGAACAA TTCTTTTAAT GAGCAGGTAT TTGCAATATT GAAGTATGAA TATGCAATAT TAATAGTTGG AAATCTTTCA TTATTGATGA CAGTGGATTC TATTATAAAG AAGCTAAACA AGTCAGTAAT TTCTTTATTT TTATTTTCTC GAACATAAGT TACAATAACT TTATCAATAT TCGTGAAATA ATGTTTAACA TCTTCAACAT TAAAGCATTG ATGAATTGTG ATTTTGTCTT GCAAATCTTC AATCTTATCC AGCTTCATAT ATGCTTGTTT TCCAAGCTCT TTTGAATGGT TAATTTCATC ATAGTTTATC AGATTATTCA TGGTTTCGTT GCTGACATCT TTATATTTCA TAAAGTAATT TTCGAATAAT TTACAGATTT GTTCTGCATC AGAATCTACG GAGGGTCTAA ACCCTTCAAT TCTTTTATCA GCAGGAATAC GATGATATTT AATTAAAGAG CTCATGGTCA TATTTCTTGG GATTCCAATA AAACCAGCTT CTAATAATTT CTTTACATTA ATTATACGAT GCCAATAAGT TCCAATAGTA AAAGGTTTGG TAATATTCTT CCCACAAGTG TAAATTGATT GAAAGATTTT TTCACATCTG ATTCTTCTTG TAATTTCCTT AATAAGTACA GGGGCAAGTC TTTTAGATCT AAGCTTCTTA TGAACGCAAA GAAAATTAAC TACAGAAGTA TTCATAATGC AATTATGAAT TCTAACTTTG ATTGGGATTG CGGAAATAAA TCCAACCATT TTCTTTGTTT CATTAACTCT GACTCCAACA AGCCAGTTCT TTTTTTGACC AGGGACACAA AGAGCCCATC TAAGAAACTC CTTTGAATAA TTAAATCTAA ATTGGGAATC GTCATCTTCA ACATAATGGT CTTTTAATAA TTGGTAAGTA TCTTCAAAAT CTTGAGATTC TATATCCCAT AAATTACAAT CAAACCAAGA GAATCCATCT GGTAGCTTAT ATATTTCTTT TCTAAAAGAA TCTGGTTCAA TTTCAATGGG TCCAAATGAA TATTCACTGG AAGAATCATC GTTTTGAACT ACTGGTTGAG TATTCCAGAA TTTGTGTGGT TTGATTGCAT TTGCGGCAGT GTTCATAAAG CTACCTAAGC TCATTTCTTT CAGAAGATTT GTGATCTTCT TGGTATTTGA AATGGAAGAT TCTGAATTCT CTGGGGTTTC ACCACTCATT CTAATAAGCA TATGGTGGTG GTGGTGGTGA GCCAT
MAHHHHHHML IRMSGETPEN SESSISNTKK ITNLLKEMSL GSFMNTAANA IKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTA TTAGAATGAG TGGTGAAACC CCAGAGAATT CAGAATCTTC CATTTCAAAT ACCAAGAAGA TCACAAATCT TCTGAAAGAA ATGAGCTTAG GTAGCTTTAT GAACACTGCC GCAAATGCAA TCAAACCACA CAAATTCTGG AATACTCAAC CAGTAGTTCA AAACGATGAT TCTTCCAGTG AATATTCATT TGGACCCATT GAAATTGAAC CAGATTCTTT TAGAAAAGAA ATATATAAGC TACCAGATGG ATTCTCTTGG TTTGATTGTA ATTTATGGGA TATAGAATCT CAAGATTTTG AAGATACTTA CCAATTATTA AAAGACCATT ATGTTGAAGA TGACGATTCC CAATTTAGAT TTAATTATTC AAAGGAGTTT CTTAGATGGG CTCTTTGTGT CCCTGGTCAA AAAAAGAACT GGCTTGTTGG AGTCAGAGTT AATGAAACAA AGAAAATGGT TGGATTTATT TCCGCAATCC CAATCAAAGT TAGAATTCAT AATTGCATTA TGAATACTTC TGTAGTTAAT TTTCTTTGCG TTCATAAGAA GCTTAGATCT AAAAGACTTG CCCCTGTACT TATTAAGGAA ATTACAAGAA GAATCAGATG TGAAAAAATC TTTCAATCAA TTTACACTTG TGGGAAGAAT ATTACCAAAC CTTTTACTAT TGGAACTTAT TGGCATCGTA TAATTAATGT AAAGAAATTA TTAGAAGCTG GTTTTATTGG AATCCCAAGA AATATGACCA TGAGCTCTTT AATTAAATAT CATCGTATTC CTGCTGATAA AAGAATTGAA GGGTTTAGAC CCTCCGTAGA TTCTGATGCA GAACAAATCT GTAAATTATT CGAAAATTAC TTTATGAAAT ATAAAGATGT CAGCAACGAA ACCATGAATA ATCTGATAAA CTATGATGAA ATTAACCATT CAAAAGAGCT TGGAAAACAA GCATATATGA AGCTGGATAA GATTGAAGAT TTGCAAGACA AAATCACAAT TCATCAATGC TTTAATGTTG AAGATGTTAA ACATTATTTC ACGAATATTG ATAAAGTTAT TGTAACTTAT GTTCGAGAAA ATAAAAATAA AGAAATTACT GACTTGTTTA GCTTCTTTAT AATAGAATCC ACTGTCATCA ATAATGAAAG ATTTCCAACT ATTAATATTG CATATTCATA CTTCAATATT GCAAATACCT GCTCATTAAA AGAATTGTTC AATGAAATGC TTATTACTGC CAAAAATAAT AATTGTGATG CTTTTAATAC ATTGGATCTG ATGCAAAATT TACAAGTTAT TCAAGATAGT AAATTTATTA TCGGTACCGG TAGATTAAGA TATTATGTGT TTAATTGGAA GATACCTCAA ATTTCACCTT CAAATGTTGG AATTATATTA TTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
purified on: 1/12/2016
concentration: 33mg/ml
observed molecular weight:
48kDa
expression level:
Moderate Expression
protein purification buffer:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
expression host:
data unavailable
vial count (approx.):
8
vial volume:
200µl
percent identity: data unavailable
percent coverage: data unavailable
MAHHHHHHMG TLEAQTQGPG SIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT CAAACCACAC AAATTCTGGA ATACTCAACC AGTAGTTCAA AACGATGATT CTTCCAGTGA ATATTCATTT GGACCCATTG AAATTGAACC AGATTCTTTT AGAAAAGAAA TATATAAGCT ACCAGATGGA TTCTCTTGGT TTGATTGTAA TTTATGGGAT ATAGAATCTC AAGATTTTGA AGATACTTAC CAATTATTAA AAGACCATTA TGTTGAAGAT GACGATTCCC AATTTAGATT TAATTATTCA AAGGAGTTTC TTAGATGGGC TCTTTGTGTC CCTGGTCAAA AAAAGAACTG GCTTGTTGGA GTCAGAGTTA ATGAAACAAA GAAAATGGTT GGATTTATTT CCGCAATCCC AATCAAAGTT AGAATTCATA ATTGCATTAT GAATACTTCT GTAGTTAATT TTCTTTGCGT TCATAAGAAG CTTAGATCTA AAAGACTTGC CCCTGTACTT ATTAAGGAAA TTACAAGAAG AATCAGATGT GAAAAAATCT TTCAATCAAT TTACACTTGT GGGAAGAATA TTACCAAACC TTTTACTATT GGAACTTATT GGCATCGTAT AATTAATGTA AAGAAATTAT TAGAAGCTGG TTTTATTGGA ATCCCAAGAA ATATGACCAT GAGCTCTTTA ATTAAATATC ATCGTATTCC TGCTGATAAA AGAATTGAAG GGTTTAGACC CTCCGTAGAT TCTGATGCAG AACAAATCTG TAAATTATTC GAAAATTACT TTATGAAATA TAAAGATGTC AGCAACGAAA CCATGAATAA TCTGATAAAC TATGATGAAA TTAACCATTC AAAAGAGCTT GGAAAACAAG CATATATGAA GCTGGATAAG ATTGAAGATT TGCAAGACAA AATCACAATT CATCAATGCT TTAATGTTGA AGATGTTAAA CATTATTTCA CGAATATTGA TAAAGTTATT GTAACTTATG TTCGAGAAAA TAAAAATAAA GAAATTACTG ACTTGTTTAG CTTCTTTATA ATAGAATCCA CTGTCATCAA TAATGAAAGA TTTCCAACTA TTAATATTGC ATATTCATAC TTCAATATTG CAAATACCTG CTCATTAAAA GAATTGTTCA ATGAAATGCT TATTACTGCC AAAAATAATA ATTGTGATGC TTTTAATACA TTGGATCTGA TGCAAAATTT ACAAGTTATT CAAGATAGTA AATTTATTAT CGGTACCGGT AGATTAAGAT ATTATGTGTT TAATTGGAAG ATACCTCAAA TTTCACCTTC AAATGTTGGA ATTATATTAT TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
purified on: 12/2/2021
concentration: 86mg/ml
observed molecular weight:
50kDa
expression level:
Moderate Expression
protein purification buffer:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
expression host:
BL 21 (DE3) Rosetta
vial count (approx.):
12
vial volume:
25µl
percent identity: 95
percent coverage: 66
MGTLEAQTQG PGSKPHKFWN TQPVVQNDDS SSEYSFGPIE IEPDSFRKEI YKLPDGFSWF DCNLWDIESQ DFEDTYQLLK DHYVEDDDSQ FRFNYSKEFL RWALCVPGQK KNWLVGVRVN ETKKMVGFIS AIPIKVRIHN CIMNTSVVNF LCVHKKLRSK RLAPVLIKEI TRRIRCEKIF QSIYTCGKNI TKPFTIGTYW HRIINVKKLL EAGFIGIPRN MTMSSLIKYH RIPADKRIEG FRPSVDSDAE QICKLFENYF MKYKDVSNET MNNLINYDEI NHXXXXXXTS IYXXGXX
ANGGNCTCAT CACCATCACC ATCATATGGG TACCCTGGAA GCTCAGACCC AGGGTCCTGG TTCGAAACCA CACAAATTCT GGAATACTCA ACCAGTAGTT CAAAACGATG ATTCTTCCAG TGAATATTCA TTTGGACCCA TTGAAATTGA ACCAGATTCT TTTAGAAAAG AAATATATAA GCTACCAGAT GGATTCTCTT GGTTTGATTG TAATTTATGG GATATAGAAT CTCAAGATTT TGAAGATACT TACCAATTAT TAAAAGACCA TTATGTTGAA GATGACGATT CCCAATTTAG ATTTAATTAT TCAAAGGAGT TTCTTAGATG GGCTCTTTGT GTCCCTGGTC AAAAAAAGAA CTGGCTTGTT GGAGTCAGAG TTAATGAAAC AAAGAAAATG GTTGGATTTA TTTCCGCAAT CCCAATCAAA GTTAGAATTC ATAATTGCAT TATGAATACT TCTGTAGTTA ATTTTCTTTG CGTTCATAAG AAGCTTAGAT CTAAAAGACT TGCCCCTGTA CTTATTAAGG AAATTACAAG AAGAATCAGA TGTGAAAAAA TCTTTCAATC AATTTACACT TGTGGGAAGA ATATTACCAA ACCTTTTACT ATTGGAACTT ATTGGCATCG TATAATTAAT GTAAAGAAAT TATTAGAAGC TGGTTTTATT GGAATCCCAA GAAATATGAC CATGAGCTCT TTAATTAAAT ATCATCGTAT TCCTGCTGAT AAAAGAATTG AAGGGTTTAG ACCCTCCGTA GATTCTGATG CAGAACAAAT CTGTAAATTA TTCGAAAATT ACTTTATGAA ATATAAAGAT GTCAGCAACG AAACCATGAA TAATCTGATA AACTATGATG AAATTAACCA TNNNNANNNN CNNNNGNAAA CAAGCATATA TNNNNNTGGA NNNNNTTGAN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ATNNNNNNNN NNNNNNNNNN NNAANNNNNA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA ANNNNNNNNN NNT
MAHHHHHHMG TLEAQTQGPG SKPHKFWNTQ PVVQNDDSSS EYSFGPIEIE PDSFRKEIYK LPDGFSWFDC NLWDIESQDF EDTYQLLKDH YVEDDDSQFR FNYSKEFLRW ALCVPGQKKN WLVGVRVNET KKMVGFISAI PIKVRIHNCI MNTSVVNFLC VHKKLRSKRL APVLIKEITR RIRCEKIFQS IYTCGKNITK PFTIGTYWHR IINVKKLLEA GFIGIPRNMT MSSLIKYHRI PADKRIEGFR PSVDSDAEQI CKLFENYFMK YKDVSNETMN NLINYDEINH SKELGKQAYM KLDKIEDLQD KITIHQCFNV EDVKHYFTNI DKVIVTYVRE NKNKEITDLF SFFIIESTVI NNERFPTINI AYSYFNIANT CSLKELFNEM LITAKNNNCD AFNTLDLMQN LQVIQDSKFI IGTGRLRYYV FNWKIPQISP SNVGIILF
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAA ACCACACAAA TTCTGGAATA CTCAACCAGT AGTTCAAAAC GATGATTCTT CCAGTGAATA TTCATTTGGA CCCATTGAAA TTGAACCAGA TTCTTTTAGA AAAGAAATAT ATAAGCTACC AGATGGATTC TCTTGGTTTG ATTGTAATTT ATGGGATATA GAATCTCAAG ATTTTGAAGA TACTTACCAA TTATTAAAAG ACCATTATGT TGAAGATGAC GATTCCCAAT TTAGATTTAA TTATTCAAAG GAGTTTCTTA GATGGGCTCT TTGTGTCCCT GGTCAAAAAA AGAACTGGCT TGTTGGAGTC AGAGTTAATG AAACAAAGAA AATGGTTGGA TTTATTTCCG CAATCCCAAT CAAAGTTAGA ATTCATAATT GCATTATGAA TACTTCTGTA GTTAATTTTC TTTGCGTTCA TAAGAAGCTT AGATCTAAAA GACTTGCCCC TGTACTTATT AAGGAAATTA CAAGAAGAAT CAGATGTGAA AAAATCTTTC AATCAATTTA CACTTGTGGG AAGAATATTA CCAAACCTTT TACTATTGGA ACTTATTGGC ATCGTATAAT TAATGTAAAG AAATTATTAG AAGCTGGTTT TATTGGAATC CCAAGAAATA TGACCATGAG CTCTTTAATT AAATATCATC GTATTCCTGC TGATAAAAGA ATTGAAGGGT TTAGACCCTC CGTAGATTCT GATGCAGAAC AAATCTGTAA ATTATTCGAA AATTACTTTA TGAAATATAA AGATGTCAGC AACGAAACCA TGAATAATCT GATAAACTAT GATGAAATTA ACCATTCAAA AGAGCTTGGA AAACAAGCAT ATATGAAGCT GGATAAGATT GAAGATTTGC AAGACAAAAT CACAATTCAT CAATGCTTTA ATGTTGAAGA TGTTAAACAT TATTTCACGA ATATTGATAA AGTTATTGTA ACTTATGTTC GAGAAAATAA AAATAAAGAA ATTACTGACT TGTTTAGCTT CTTTATAATA GAATCCACTG TCATCAATAA TGAAAGATTT CCAACTATTA ATATTGCATA TTCATACTTC AATATTGCAA ATACCTGCTC ATTAAAAGAA TTGTTCAATG AAATGCTTAT TACTGCCAAA AATAATAATT GTGATGCTTT TAATACATTG GATCTGATGC AAAATTTACA AGTTATTCAA GATAGTAAAT TTATTATCGG TACCGGTAGA TTAAGATATT ATGTGTTTAA TTGGAAGATA CCTCAAATTT CACCTTCAAA TGTTGGAATT ATATTATTTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA