CrpaA.18219.a
N-myristoyltransferase
CENTER ID:
CrpaA.18219.a
ORGANISM:
Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:
cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
CrpaA.18219.a.A10.GE42168
truncation
info
43
469
CrpaA.18219.a.A10.GE42168
CrpaA.18219.a.A11.GE42169
truncation
info
44
469
CrpaA.18219.a.A11.GE42169
CrpaA.18219.a.A12.GE42170
truncation
info
45
469
CrpaA.18219.a.A12.GE42170
CrpaA.18219.a.A3.GE39566
truncation 2
info
84
469
CrpaA.18219.a.A3.GE39566
CrpaA.18219.a.A4.GE39567
truncation
info
42
469
CrpaA.18219.a.A4.GE39567
CrpaA.18219.a.A41.GE42008
truncation
info
42
469
CrpaA.18219.a.A41.GE42008
CrpaA.18219.a.A42.GE42009
truncation
info
42
469
CrpaA.18219.a.A42.GE42009
CrpaA.18219.a.A6.GE39568
truncation
info
92
469
CrpaA.18219.a.A6.GE39568
CrpaA.18219.a.A7.GE39569
truncation
info
79
469
CrpaA.18219.a.A7.GE39569
CrpaA.18219.a.A8.GE42171
truncation
info
40
469
CrpaA.18219.a.A8.GE42171
CrpaA.18219.a.A9.GE42172
truncation
info
41
469
CrpaA.18219.a.A9.GE42172
CrpaA.18219.a.B1.GE35467
full length
info
1
469
CrpaA.18219.a.B1.GE35467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
CrpaA.18219.a.B1.PS38190
full length
info
1
469
PS38190
CrpaA.18219.a.B1.PW37298
full length
info
1
469
PW37298
CrpaA.18219.a.A4.PW37835
truncation
info
42
469
PW37835
CrpaA.18219.a.A7.PW37838
truncation
info
79
469
PW37838
CrpaA.18219.a.A10.PW39122
truncation
info
43
469
PW39122
Structures
8FBM
DEPOSITED: 11/29/2022
DETERMINATION: XRay
CLONE: CrpaA.18219.a.A10.GE42168
PROTEIN: CrpaA.18219.a.A10.PS38408
Publications by SSGCID
Identification of and structural insights into hit compounds targeting N-myristoyltranserase for Cryptosporidium drug development
Fan E, Myler PJ, Sankaran B, Staker BL, Van Voorhis WC, Hulverson MA, Reers A, Hammerson B, FernándezÁlvaro E, Kaushansky A, Zigweid R, Fenwick MK, Liu Y, Shin J
ACS Infect Dis - 2023
volume 9, issue 10, pages 1821-1833
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LIRMSGETPE NSESSISNTK KITNLLKEMS LGSFMNTAAN AIKPHKFWNT QPVVQNDDSS SEYSFGPIEI EPDSFRKEIY KLPDGFSWFD CNLWDIESQD FEDTYQLLKD HYVEDDDSQF RFNYSKEFLR WALCVPGQKK NWLVGVRVNE TKKMVGFISA IPIKVRIHNC IMNTSVVNFL CVHKKLRSKR LAPVLIKEIT RRIRCEKIFQ SIYTCGKNIT KPFTIGTYWH RIINVKKLLE AGFIGIPRNM TMSSLIKYHR IPADKRIEGF RPSVDSDAEQ ICKLFENYFM KYKDVSNETM NNLINYDEIN HSKELGKQAY MKLDKIEDLQ DKITIHQCFN VEDVKHYFTN IDKVIVTYVR ENKNKEITDL FSFFIIESTV INNERFPTIN IAYSYFNIAN TCSLKELFNE MLITAKNNNC DAFNTLDLMQ NLQVIQDSKF IIGTGRLRYY VFNWKIPQIS PSNVGIILF
NT Sequence
cttattagaa tgagtggtga aaccccagag aattcagaat cttccatttc aaataccaag aagatcacaa atcttctgaa agaaatgagc ttaggtagct ttatgaacac tgccgcaaat gcaatcaaac cacacaaatt ctggaatact caaccagtag ttcaaaacga tgattcttcc agtgaatatt catttggacc cattgaaatt gaaccagatt cttttagaaa agaaatatat aagctaccag atggattctc ttggtttgat tgtaatttat gggatataga atctcaagat tttgaagata cttaccaatt attaaaagac cattatgttg aagatgacga ttcccaattt agatttaatt attcaaagga gtttcttaga tgggctcttt gtgtccctgg tcaaaaaaag aactggcttg ttggagtcag agttaatgaa acaaagaaaa tggttggatt tatttccgca atcccaatca aagttagaat tcataattgc attatgaata cttctgtagt taattttctt tgcgttcata agaagcttag atctaaaaga cttgcccctg tacttattaa ggaaattaca agaagaatca gatgtgaaaa aatctttcaa tcaatttaca cttgtgggaa gaatattacc aaacctttta ctattggaac ttattggcat cgtataatta atgtaaagaa attattagaa gctggtttta ttggaatccc aagaaatatg accatgagct ctttaattaa atatcatcgt attcctgctg ataaaagaat tgaagggttt agaccctccg tagattctga tgcagaacaa atctgtaaat tattcgaaaa ttactttatg aaatataaag atgtcagcaa cgaaaccatg aataatctga taaactatga tgaaattaac cattcaaaag agcttggaaa acaagcatat atgaagctgg ataagattga agatttgcaa gacaaaatca caattcatca atgctttaat gttgaagatg ttaaacatta tttcacgaat attgataaag ttattgtaac ttatgttcga gaaaataaaa ataaagaaat tactgacttg tttagcttct ttataataga atccactgtc atcaataatg aaagatttcc aactattaat attgcatatt catacttcaa tattgcaaat acctgctcat taaaagaatt gttcaatgaa atgcttatta ctgccaaaaa taataattgt gatgctttta atacattgga tctgatgcaa aatttacaag ttattcaaga tagtaaattt attatcggta ccggtagatt aagatattat gtgtttaatt ggaagatacc tcaaatttca ccttcaaatg ttggaattat attattttag
Details for CrpaA.18219.a.A10.GE42168
HARVESTED ON:
12/14/2017
SEQUENCED ON:
12/6/2017
EXPECTED MW:
53kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
95
PERCENT COVERAGE:
66
Details for CrpaA.18219.a.A11.GE42169
HARVESTED ON:
1/8/2018
SEQUENCED ON:
12/12/2017
EXPECTED MW:
52kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
86
PERCENT COVERAGE:
75
Details for CrpaA.18219.a.A12.GE42170
HARVESTED ON:
12/14/2017
SEQUENCED ON:
12/6/2017
EXPECTED MW:
52kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
98
Details for CrpaA.18219.a.A3.GE39566
HARVESTED ON:
10/23/2015
SEQUENCED ON:
10/23/2015
EXPECTED MW:
48kDa
OBSERVED MW:
48kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18219.a.A4.GE39567
HARVESTED ON:
10/23/2015
SEQUENCED ON:
10/23/2015
EXPECTED MW:
53kDa
OBSERVED MW:
53kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18219.a.A41.GE42008
HARVESTED ON:
7/27/2017
SEQUENCED ON:
7/27/2017
EXPECTED MW:
53kDa
OBSERVED MW:
53kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
98
Details for CrpaA.18219.a.A42.GE42009
HARVESTED ON:
7/27/2017
SEQUENCED ON:
7/27/2017
EXPECTED MW:
53kDa
OBSERVED MW:
53kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
99
PERCENT COVERAGE:
95
Details for CrpaA.18219.a.A6.GE39568
HARVESTED ON:
10/23/2015
SEQUENCED ON:
10/23/2015
EXPECTED MW:
47kDa
OBSERVED MW:
47kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18219.a.A7.GE39569
HARVESTED ON:
10/23/2015
SEQUENCED ON:
10/23/2015
EXPECTED MW:
48kDa
OBSERVED MW:
48kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
99
Details for CrpaA.18219.a.A8.GE42171
HARVESTED ON:
12/14/2017
SEQUENCED ON:
12/6/2017
EXPECTED MW:
53kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18219.a.A9.GE42172
HARVESTED ON:
1/8/2018
SEQUENCED ON:
12/12/2017
EXPECTED MW:
53kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
89
PERCENT COVERAGE:
72
Details for CrpaA.18219.a.B1.GE35467
HARVESTED ON:
6/26/2012
SEQUENCED ON:
1/25/2017
EXPECTED MW:
56kDa
OBSERVED MW:
56kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for CrpaA.18219.a.B1.PS38190
PURIFICATION DATe:
2/7/2017
CONCENTRATION:
23.1mg/ml
OBSERVED MW:
60kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
128
VIAL VOLUME:
6µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for CrpaA.18219.a.B1.PW37298
PURIFICATION DATe:
5/28/2013
CONCENTRATION:
17.5mg/ml
OBSERVED MW:
48kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
1
VIAL VOLUME:
110µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for CrpaA.18219.a.A4.PW37835
PURIFICATION DATe:
1/12/2016
CONCENTRATION:
33mg/ml
OBSERVED MW:
48kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for CrpaA.18219.a.A7.PW37838
PURIFICATION DATe:
1/13/2016
CONCENTRATION:
25.8mg/ml
OBSERVED MW:
45kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
110µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
99
Details for CrpaA.18219.a.A10.PW39122
PURIFICATION DATe:
12/2/2021
CONCENTRATION:
86mg/ml
OBSERVED MW:
50kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:
BL 21 (DE3) Rosetta
VIAL COUNT (approx.):
12
VIAL VOLUME:
25µl
PERCENT IDENTITY:
95
PERCENT COVERAGE:
66