CrpaA.18216.b

ruvB-like 1, putative

CENTER ID: CrpaA.18216.b
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.18216.b.B1.GE35470 full length 1 457
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd7_2090
RefSeq: XP_001388382.1
UniProt: A3FPN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIEGEKPSS TNFSRVSTHS HIRGLGLKND GTASNDGSCG MIGQLQARES AGVVLSLIQN KKLAGKAVLL AGPPGTGKTA IAQAIAHELG PKVPFCPMVA SEVYSAEVKK TEILMENFRR AIGLRIRDVK EVYEGEVVEL VTEETENPHG NFGKAVSAIV LTLKSAKGTK TLRLAPQLSD VFQKEKVKVG DIIYVESSSG IVKRLGRSDS FATEFDLESE EYVPLPKGDV YKKREIVQDI TLYDLDLANA KPQGGQDIIS LLGQYVRPKK TEITEKLRLE VNKSVNEYID QGVAELVPGV LFIDEVHMLD IECFTFLNRT LESSLAPIVI FGTNRGVCTV RGTDMLSSHG IPVDLLDRLL IIRTIPYNIE EMIRIVSIRC DIEGIKMDKE SLQLIGEIGS STSLRYICQL LTPAHIIAST FGRDTICKSD IQEVDSLFFD SNASARRLAE DSNSFIL
NT Sequence
atgaacatag agggcgaaaa gccaagctca acaaatttta gccgggtttc tactcatagc catattagag gattgggttt gaagaatgat gggacagcat caaatgacgg atcatgcgga atgatcggcc aactacaagc cagggaatct gcaggggttg ttttgagttt aatacagaat aaaaagttgg cagggaaggc tgtattattg gctggcccac ctggtactgg taagacagca attgcccaag ctatagcgca tgaattaggt ccaaaagttc cattttgtcc aatggtggca tctgaagtgt attcagctga ggttaaaaag actgaaatac ttatggaaaa ttttagaaga gctattggac ttaggattag agatgttaaa gaggtatatg aaggcgaggt tgttgaatta gtcactgaag aaactgaaaa cccccatgga aactttggaa aggcagtatc agcaatagta ttgactttaa aatcagcaaa gggaacaaaa acgcttcgtc ttgctcctca attaagtgat gtttttcaaa aagagaaggt aaaggttgga gacataattt atgttgaatc ttctagcgga attgttaaga gattaggtag atcagattca tttgccacag agtttgactt agaatctgaa gagtatgttc ctctcccaaa gggagacgtt tataaaaaga gggaaattgt tcaagatatt accttgtatg atctagattt ggcaaatgct aaacctcaag gtggccaaga tatcatttca ttattggggc aatacgtacg tccaaagaag acagaaataa ctgagaaact tagactagaa gttaacaaaa gtgtaaatga gtatattgac caaggagtgg cggagcttgt cccaggtgta ttgtttattg atgaagtaca catgctcgat attgagtgtt ttacgttcct taatagaact ctcgaaagct ctctggctcc tattgttatt tttggaacca atagaggcgt atgtaccgtt agaggtactg atatgctttc ttcgcatgga attcctgttg atctattgga cagacttcta ataattagaa caattccata taatattgaa gagatgatta gaattgtttc tattaggtgc gatattgagg ggataaaaat ggataaggag tctttacagc ttattggaga aatcggtagc tcaacatcat tacgttatat atgtcagtta ttgactcctg ctcatattat tgcaagcact tttggaagag ataccatttg caaatctgat attcaggagg tagatagtct ctttttcgat tctaatgcat ctgctagaag attggctgaa gactctaatt catttatcct ctag
Details for CrpaA.18216.b.B1.GE35470
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MIGQLQARES AGVVLSLIQN KKLAGKAVLL AGPPGTGKTA IAQAIAHELG PKVPFCPMVA SEVYSAEVKK TEILMENFRR AIGLRIRDVK EVYEGEVVEL VTEETENPHG NFGKAVSAIV LTLKSAKGTK TLRLAPQLSD VFQKEKVKVG DIIYVESSSG IVKRLGRSDS FATEFDLESE EYVPLPKGDV YKKREIVQDI TLYDLDLANA KPQGGQDIIS LLGQYVRPKK TEITEKLRLE VNKSVNEYID QGVAELVPGV LFIDEVHMLD IECFTFLNRT LESSLAPIVI FGTNRGVCTV RGTDMLSSHG IPVDLLDRLL IIRTIPYNIE EMIRIVSIRC DIEGIKMDKE SLQLIGEIGS STSLRYICQL LTPAHIIAST FGRDTICKSD IQEVDSLFFD S
Validated NT Sequence
ttagaatcga aaaagagact atctacctcc tgaatatcag atttgcaaat ggtatctctt ccaaaagtgc ttgcaataat atgagcagga gtcaataact gacatatata acgtaatgat gttgagctac cgatttctcc aataagctgt aaagactcct tatccatttt tatcccctca atatcgcacc taatagaaac aattctaatc atctcttcaa tattatatgg aattgttcta attattagaa gtctgtccaa tagatcaaca ggaattccat gcgaagaaag catatcagta cctctaacgg tacatacgcc tctattggtt ccaaaaataa caataggagc cagagagctt tcgagagttc tattaaggaa cgtaaaacac tcaatatcga gcatgtgtac ttcatcaata aacaatacac ctgggacaag ctccgccact ccttggtcaa tatactcatt tacacttttg ttaacttcta gtctaagttt ctcagttatt tctgtcttct ttggacgtac gtattgcccc aataatgaaa tgatatcttg gccaccttga ggtttagcat ttgccaaatc tagatcatac aaggtaatat cttgaacaat ttccctcttt ttataaacgt ctccctttgg gagaggaaca tactcttcag attctaagtc aaactctgtg gcaaatgaat ctgatctacc taatctctta acaattccgc tagaagattc aacataaatt atgtctccaa cctttacctt ctctttttga aaaacatcac ttaattgagg agcaagacga agcgtttttg ttccctttgc tgattttaaa gtcaatacta ttgctgatac tgcctttcca aagtttccat gggggttttc agtttcttca gtgactaatt caacaacctc gccttcatat acctctttaa catctctaat cctaagtcca atagctcttc taaaattttc cataagtatt tcagtctttt taacctcagc tgaatacact tcagatgcca ccattggaca aaatggaact tttggaccta attcatgcgc tatagcttgg gcaattgctg tcttaccagt accaggtggg ccagccaata atacagcctt ccctgccaac tttttattct gtattaaact caaaacaacc cctgcagatt ccctggcttg tagttggccg atcattccgc atgatccgtc atttgatgct gtcccatcat tcttcaaacc caatcctcta atatggctat gagtagaaac ccggctaaaa tttgttgagc ttggcttttc gccctc
Expected Protein Sequence
MAHHHHHHMN IEGEKPSSTN FSRVSTHSHI RGLGLKNDGT ASNDGSCGMI GQLQARESAG VVLSLIQNKK LAGKAVLLAG PPGTGKTAIA QAIAHELGPK VPFCPMVASE VYSAEVKKTE ILMENFRRAI GLRIRDVKEV YEGEVVELVT EETENPHGNF GKAVSAIVLT LKSAKGTKTL RLAPQLSDVF QKEKVKVGDI IYVESSSGIV KRLGRSDSFA TEFDLESEEY VPLPKGDVYK KREIVQDITL YDLDLANAKP QGGQDIISLL GQYVRPKKTE ITEKLRLEVN KSVNEYIDQG VAELVPGVLF IDEVHMLDIE CFTFLNRTLE SSLAPIVIFG TNRGVCTVRG TDMLSSHGIP VDLLDRLLII RTIPYNIEEM IRIVSIRCDI EGIKMDKESL QLIGEIGSST SLRYICQLLT PAHIIASTFG RDTICKSDIQ EVDSLFFDSN ASARRLAEDS NSFIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca tagagggcga aaagccaagc tcaacaaatt ttagccgggt ttctactcat agccatatta gaggattggg tttgaagaat gatgggacag catcaaatga cggatcatgc ggaatgatcg gccaactaca agccagggaa tctgcagggg ttgttttgag tttaatacag aataaaaagt tggcagggaa ggctgtatta ttggctggcc cacctggtac tggtaagaca gcaattgccc aagctatagc gcatgaatta ggtccaaaag ttccattttg tccaatggtg gcatctgaag tgtattcagc tgaggttaaa aagactgaaa tacttatgga aaattttaga agagctattg gacttaggat tagagatgtt aaagaggtat atgaaggcga ggttgttgaa ttagtcactg aagaaactga aaacccccat ggaaactttg gaaaggcagt atcagcaata gtattgactt taaaatcagc aaagggaaca aaaacgcttc gtcttgctcc tcaattaagt gatgtttttc aaaaagagaa ggtaaaggtt ggagacataa tttatgttga atcttctagc ggaattgtta agagattagg tagatcagat tcatttgcca cagagtttga cttagaatct gaagagtatg ttcctctccc aaagggagac gtttataaaa agagggaaat tgttcaagat attaccttgt atgatctaga tttggcaaat gctaaacctc aaggtggcca agatatcatt tcattattgg ggcaatacgt acgtccaaag aagacagaaa taactgagaa acttagacta gaagttaaca aaagtgtaaa tgagtatatt gaccaaggag tggcggagct tgtcccaggt gtattgttta ttgatgaagt acacatgctc gatattgagt gttttacgtt ccttaataga actctcgaaa gctctctggc tcctattgtt atttttggaa ccaatagagg cgtatgtacc gttagaggta ctgatatgct ttcttcgcat ggaattcctg ttgatctatt ggacagactt ctaataatta gaacaattcc atataatatt gaagagatga ttagaattgt ttctattagg tgcgatattg aggggataaa aatggataag gagtctttac agcttattgg agaaatcggt agctcaacat cattacgtta tatatgtcag ttattgactc ctgctcatat tattgcaagc acttttggaa gagataccat ttgcaaatct gatattcagg aggtagatag tctctttttc gattctaatg catctgctag aagattggct gaagactcta attcatttat cctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat