CrpaA.18216.a

ruvB-like 2 protein, putative

CENTER ID: CrpaA.18216.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.18216.a.B1.GE35465 full length 1 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd5_670
RefSeq: XP_626023.1
UniProt: Q5CS13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEPKIAEIK EFQRLERIGA HSHIRGLGLN DALDARYSSD GMVGQKLARR AAGIIVRMVK QGKIAGRAIL LSGQPGTGKT AIAMAIAKAI GSDTPFTHIS ASEVFSLEMN KTEALRQALR RSIGVRIKEE IDVIEGEVAE LEIDRSNTTG VKVGRMALRS TDMETVYDIG SKMIESLQAE NIVAGDVISI NKSSGKITKL GRSFTRSKDY DAVGYQTRFI ACPEGELQKK REVVHNVTLH DIDVINSRTQ GFLALFAGDT GEIKPEVRAQ IDEKVAEWKE ESRAEIVHGV LFIDEVHMLD VECFSFLNKA LEEETSPILI MASNRGITKI RGTDYKSPHG IPIDLLDRCL IIPTVPYSEE DVKKIIQERA SEEDLKLTDS AYQILTRIAM DTSLRYSLHL LTVSQVLANR KKKEEIDLDE IKKAYSLFID VKRSTQYLID YQQEYLFSEI EHSNNDVKDK DNGNDTMRDT AVQEKMTLI
NT Sequence
atgattgaac caaaaatagc agaaattaaa gagtttcaaa gactggaaag aattggggca cattcccata ttagaggcct aggtttaaat gatgcattgg atgcaaggta ttcatcagat ggaatggtgg gtcaaaagtt ggctagaaga gctgcaggaa taattgtaag aatggtcaag caaggcaaga ttgcaggaag agcgatttta ttatcgggcc aaccaggaac tggaaaaaca gcaattgcta tggcaattgc aaaagcaata ggaagtgata cgccatttac acatatttca gcttctgaag tgttctcact agaaatgaat aagactgaag ctctgagaca agctttgaga agatccatag gtgtaagaat aaaagaagaa atagacgtta ttgagggaga agtagcagaa ttagagattg atcgttcgaa tacaacagga gttaaagttg gcagaatggc tttaagaagt accgatatgg agacagttta tgatattggt agtaaaatga ttgagtcact tcaagctgaa aatattgttg ctggagatgt aatttcaatt aataagtcaa gtgggaaaat tactaaatta ggacgttctt ttacaaggtc caaagattac gatgccgttg gatatcaaac tagatttatt gcatgcccag aaggtgaact tcagaaaaaa agagaagtag tacataacgt cactttacac gatattgatg taattaatag ccgaactcaa ggatttttgg cactgtttgc tggtgacacc ggcgaaatta agccagaagt tagagctcaa atagatgaaa aggttgctga atggaaagag gaaagcagag cagaaattgt tcatggagtt ttgtttatag atgaggttca tatgttagat gttgaatgct ttagttttct taacaaggcg ctagaggaag aaacaagtcc aattttgatt atggcatcaa atagaggtat tacaaagatt agaggaactg actataagtc gccacatggt atacctattg atcttttaga tagatgttta attattccaa cagtacccta tagtgaagaa gatgttaaga aaattattca ggaaagagca agtgaggagg atcttaaatt aactgatagt gcttatcaaa tcctcactag aatagcgatg gacacgagct tgcgatactc actacactta ttaacagttt cacaagttct tgcaaataga aaaaaaaaag aagaaattga tttagacgag attaaaaaag cctattcact ttttattgat gttaagagaa gtactcaata tcttattgat taccaacaag agtatctatt ttctgagatt gaacattcaa ataatgacgt gaaagataaa gataatggca atgatacaat gagagacact gcagttcagg aaaaaatgac attaatttag
Details for CrpaA.18216.a.B1.GE35465
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 88
Validated AA Sequence
MVGQKLARRA AGIIVRMVKQ GKIAGRAILL SGQPGTGKTA IAMAIAKAIG SDTPFTHISA SEVFSLEMNK TEALRQALRR SIGVRIKEEI DVIEGEVAEL EIDRSNTTGV KVGRMALRST DMETVYDIGS KMIESLQAEN IVAGDVISIN KSSGKITKLG RSFTRSKDYD AVGYQTRFIA CPEGELQKKR EVVHNVTLHD IDVINSRTQG FLALFAGDTG EIKPEVRAQI DEKVAEWKEE SRAEIVHGVL FIDEVHMLDV ECFSFLNKAL EEETSPILIM ASNRGITKIR GTDYKSPXXI PIDLLDRCLI IPTVPYSEED VKKIIQERAS EEDLKLTDSA YQILTRIAMD TSLRYSLHLL TVSQVLANRK KKEEIDLDEI KKAYSLFIDV KRSTQYLIDY QQEYLFSEIE HSNNDVKDKD NGNDTMRDTA V
Validated NT Sequence
ctgaactgca gtgtctctca ttgtatcatt gccattatct ttatctttca cgtcattatt tgaatgttca atctcagaaa atagatactc ttgttggtaa tcaataagat attgagtact tctcttaaca tcaataaaaa gtgaataggc ttttttaatc tcgtctaaat caatttcttc tttttttttt ctatttgcaa gaacttgtga aactgttaat aagtgtagtg agtatcgcaa gctcgtgtcc atcgctattc tagtgaggat ttgataagca ctatcagtta atttaagatc ctcctcactt gctctttcct gaataatttt cttaacatct tcttcactat agggtactgt tggaataatt aaacatctat ctaaaagatc aataggtata cnnngnggcg acttatagtc agttcctcta atctttgtaa tacctctatt tgatgccata atcaaaattg gacttgtttc ttcctctagc gccttgttaa gaaaactaaa gcattcaaca tctaacatat gaacctcatc tataaacaaa actccatgaa caatttctgc tctgctttcc tctttccatt cagcaacctt ttcatctatt tgagctctaa cttctggctt aatttcgccg gtgtcaccag caaacagtgc caaaaatcct tgagttcggc tattaattac atcaatatcg tgtaaagtga cgttatgtac tacttctctt tttttctgaa gttcaccttc tgggcatgca ataaatctag tttgatatcc aacggcatcg taatctttgg accttgtaaa agaacgtcct aatttagtaa ttttcccact tgacttatta attgaaatta catctccagc aacaatattt tcagcttgaa gtgactcaat cattttacta ccaatatcat aaactgtctc catatcggta cttcttaaag ccattctgcc aactttaact cctgttgtat tcgaacgatc aatctctaat tctgctactt ctccctcaat aacgtctatt tcttctttta ttcttacacc tatggatctt ctcaaagctt gtctcagagc ttcagtctta ttcatttcta gtgagaacac ttcagaagct gaaatatgtg taaatggcgt atcacttcct attgcttttg caattgccat agcaattgct gtttttccag ttcctggttg gcccgataat aaaatcgctc ttcctgcaat cttgccttgc ttgaccattc ttacaattat tcctgcagct cttctagcca acttttgacc caccattcca tctgatgaat accttgcatc caatgcatca tttaaaccta ggcctctaat atgggaatgt gccccaattc tttccagtct ttgaaactct ttaatttctg ctatttttgg ttcaatca
Expected Protein Sequence
MAHHHHHHMI EPKIAEIKEF QRLERIGAHS HIRGLGLNDA LDARYSSDGM VGQKLARRAA GIIVRMVKQG KIAGRAILLS GQPGTGKTAI AMAIAKAIGS DTPFTHISAS EVFSLEMNKT EALRQALRRS IGVRIKEEID VIEGEVAELE IDRSNTTGVK VGRMALRSTD METVYDIGSK MIESLQAENI VAGDVISINK SSGKITKLGR SFTRSKDYDA VGYQTRFIAC PEGELQKKRE VVHNVTLHDI DVINSRTQGF LALFAGDTGE IKPEVRAQID EKVAEWKEES RAEIVHGVLF IDEVHMLDVE CFSFLNKALE EETSPILIMA SNRGITKIRG TDYKSPHGIP IDLLDRCLII PTVPYSEEDV KKIIQERASE EDLKLTDSAY QILTRIAMDT SLRYSLHLLT VSQVLANRKK KEEIDLDEIK KAYSLFIDVK RSTQYLIDYQ QEYLFSEIEH SNNDVKDKDN GNDTMRDTAV QEKMTLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg aaccaaaaat agcagaaatt aaagagtttc aaagactgga aagaattggg gcacattccc atattagagg cctaggttta aatgatgcat tggatgcaag gtattcatca gatggaatgg tgggtcaaaa gttggctaga agagctgcag gaataattgt aagaatggtc aagcaaggca agattgcagg aagagcgatt ttattatcgg gccaaccagg aactggaaaa acagcaattg ctatggcaat tgcaaaagca ataggaagtg atacgccatt tacacatatt tcagcttctg aagtgttctc actagaaatg aataagactg aagctctgag acaagctttg agaagatcca taggtgtaag aataaaagaa gaaatagacg ttattgaggg agaagtagca gaattagaga ttgatcgttc gaatacaaca ggagttaaag ttggcagaat ggctttaaga agtaccgata tggagacagt ttatgatatt ggtagtaaaa tgattgagtc acttcaagct gaaaatattg ttgctggaga tgtaatttca attaataagt caagtgggaa aattactaaa ttaggacgtt cttttacaag gtccaaagat tacgatgccg ttggatatca aactagattt attgcatgcc cagaaggtga acttcagaaa aaaagagaag tagtacataa cgtcacttta cacgatattg atgtaattaa tagccgaact caaggatttt tggcactgtt tgctggtgac accggcgaaa ttaagccaga agttagagct caaatagatg aaaaggttgc tgaatggaaa gaggaaagca gagcagaaat tgttcatgga gttttgttta tagatgaggt tcatatgtta gatgttgaat gctttagttt tcttaacaag gcgctagagg aagaaacaag tccaattttg attatggcat caaatagagg tattacaaag attagaggaa ctgactataa gtcgccacat ggtataccta ttgatctttt agatagatgt ttaattattc caacagtacc ctatagtgaa gaagatgtta agaaaattat tcaggaaaga gcaagtgagg aggatcttaa attaactgat agtgcttatc aaatcctcac tagaatagcg atggacacga gcttgcgata ctcactacac ttattaacag tttcacaagt tcttgcaaat agaaaaaaaa aagaagaaat tgatttagac gagattaaaa aagcctattc actttttatt gatgttaaga gaagtactca atatcttatt gattaccaac aagagtatct attttctgag attgaacatt caaataatga cgtgaaagat aaagataatg gcaatgatac aatgagagac actgcagttc aggaaaaaat gacattaatt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat