CrpaA.17908.a

ubiquinone/menaquinone biosynthesis methyltransferae, putative

CENTER ID: CrpaA.17908.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.17908.a.B1.GE35473 full length 1 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd1_2860
RefSeq: XP_001388175.1
UniProt: A3FQF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNLRNISKS GIGYSFCRSL VRKTHNLFGF KFLNIGIKNQ INLARAARFN STIKDSNTGE NNKYVYFGDR IVDKDTKRKL IEQVFSSVSN KYDLMNDLMS LGLHRCWKDI FVENIISPES FLRIKREKDE DFSNVTILDV AGGTGDVSFR IIDKFRKDNI EVLPNIIVVD SNNEMIEQGQ LKSIEKGDCS IIWIKDDGEQ LSQIPSNSVD VVAISFGIRN FTNIEKGLKS FYRVLKPGGR FLCLEFSKVN NPLISSIYQL YSSIAIPSLG EFVAGDRESY NYLVS
NT Sequence
atgaacaatt taagaaatat atcaaaaagt ggtattgggt atagtttttg tcgctcgtta gtcaggaaaa cacataattt gtttggattc aaatttttga atattggcat taagaatcaa attaatcttg caagagcagc taggtttaat tcaaccatta aagactcgaa cactggtgaa aataacaaat acgtttactt tggggatcgt attgtggaca aagatactaa aaggaagcta attgaacagg tttttagctc agtttcgaat aaatatgatt taatgaatga tttgatgagt cttggattac ataggtgttg gaaggacata tttgtagaga atatcatttc tcctgaatca tttttgagaa taaaaagaga gaaagacgaa gatttctcaa atgtaactat tttggatgtt gctggaggaa caggagatgt ttcttttagg attattgata agttcagaaa agataatatt gaggttttac caaatattat agttgtcgat tctaataatg aaatgattga gcaaggacag ctaaaatcaa ttgaaaaagg agactgcagc attatttgga ttaaagatga tggagaacaa cttagtcaaa ttcctagtaa cagtgtagat gttgttgcga tatcgtttgg aattcgcaac tttacaaata ttgaaaaagg cctcaaatca ttctataggg tactaaaacc tgggggaaga tttctatgtt tagagttcag caaagtcaat aatcctttaa tttcttctat ttaccaactt tactcatcca ttgcaattcc tagtcttggt gaatttgttg caggagatag agaatcatat aattatctag taagttaa
Details for CrpaA.17908.a.B1.GE35473
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 33kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN NLRNISKSGI GYSFCRSLVR KTHNLFGFKF LNIGIKNQIN LARAARFNST IKDSNTGENN KYVYFGDRIV DKDTKRKLIE QVFSSVSNKY DLMNDLMSLG LHRCWKDIFV ENIISPESFL RIKREKDEDF SNVTILDVAG GTGDVSFRII DKFRKDNIEV LPNIIVVDSN NEMIEQGQLK SIEKGDCSII WIKDDGEQLS QIPSNSVDVV AISFGIRNFT NIEKGLXSFY RVLKPGGRFL CLEFSKVNNP LISSIYQLYS SIAIPSLGEF XAXDRESYNY LVS
Validated NT Sequence
ttntttactt taagaaggag anataccatg gctcaccacc accaccacca tatgaacaat ttaagaaata tatcaaaaag tggtattggg tatagttttt gtcgctcgtt agtcaggaaa acacataatt tgtttggatt caaatttttg aatattggca ttaagaatca aattaatctt gcaagagcag ctaggtttaa ttcaaccatt aaagactcga acactggtga aaataacaaa tacgtttact ttggggatcg tattgtggac aaagatacta aaaggaagct aattgaacag gtttttagct cagtttcgaa taaatatgat ttaatgaatg atttgatgag tcttggatta cataggtgtt ggaaggacat atttgtagag aatatcattt ctcctgaatc atttttgaga ataaaaagag agaaagacga agatttctca aatgtaacta ttttggatgt tgctggagga acaggagatg tttcttttag gattattgat aagttcagaa aagataatat tgaggtttta ccaaatatta tagttgtcga ttctaataat gaaatgattg agcaaggaca gctaaaatca attgaaaaag gagactgcag cattatttgg attaaagatg atggagaaca acttagtcaa attcctagta acagtgtaga tgttgttgcg atatcgtttg gaattcgcaa ctttacaaat attgaaaaag gcctcanatc attctatagg gtactaaaac ctgggggaag atttctatgt ttagagttca gcaaagtcaa taatccttta atttcttcta tttaccaact ttactcatcc attgcaattc ctagtcttgg tgaatttgnt gcangagata gagaatcata taattatcta gtaagttaag tgagtaagan aggatncggn tgcnacaaan nccccgaang
Expected Protein Sequence
MAHHHHHHMN NLRNISKSGI GYSFCRSLVR KTHNLFGFKF LNIGIKNQIN LARAARFNST IKDSNTGENN KYVYFGDRIV DKDTKRKLIE QVFSSVSNKY DLMNDLMSLG LHRCWKDIFV ENIISPESFL RIKREKDEDF SNVTILDVAG GTGDVSFRII DKFRKDNIEV LPNIIVVDSN NEMIEQGQLK SIEKGDCSII WIKDDGEQLS QIPSNSVDVV AISFGIRNFT NIEKGLKSFY RVLKPGGRFL CLEFSKVNNP LISSIYQLYS SIAIPSLGEF VAGDRESYNY LVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca atttaagaaa tatatcaaaa agtggtattg ggtatagttt ttgtcgctcg ttagtcagga aaacacataa tttgtttgga ttcaaatttt tgaatattgg cattaagaat caaattaatc ttgcaagagc agctaggttt aattcaacca ttaaagactc gaacactggt gaaaataaca aatacgttta ctttggggat cgtattgtgg acaaagatac taaaaggaag ctaattgaac aggtttttag ctcagtttcg aataaatatg atttaatgaa tgatttgatg agtcttggat tacataggtg ttggaaggac atatttgtag agaatatcat ttctcctgaa tcatttttga gaataaaaag agagaaagac gaagatttct caaatgtaac tattttggat gttgctggag gaacaggaga tgtttctttt aggattattg ataagttcag aaaagataat attgaggttt taccaaatat tatagttgtc gattctaata atgaaatgat tgagcaagga cagctaaaat caattgaaaa aggagactgc agcattattt ggattaaaga tgatggagaa caacttagtc aaattcctag taacagtgta gatgttgttg cgatatcgtt tggaattcgc aactttacaa atattgaaaa aggcctcaaa tcattctata gggtactaaa acctggggga agatttctat gtttagagtt cagcaaagtc aataatcctt taatttcttc tatttaccaa ctttactcat ccattgcaat tcctagtctt ggtgaatttg ttgcaggaga tagagaatca tataattatc tagtaagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat