SSGCID: Target Page

CrpaA.17789.b

Membrane bound aspartyl proteinase with a signal peptide plus transmembrane domain

CENTER ID:	CrpaA.17789.b
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.17789.b.A2.GE46268	soluble domain		17	446

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd6_3820
UniProt:	Q5CWT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVFQVFFILM MLKISAFCEG LSNKIRSNAR AELGMEAILS NGLFNSNAQK VELKLSLSEG GMKYSEGNYF SRWITKSKYS ETMDSQDLRN YQNSQYFGKI EVGTPPREFV VIFDTGSSSV WIPSIECKHK GCEPHNKYDP KLSTSYQKLG DGSLETYIQY GTGSCVLKFG KEVINIGKLK IEDQSFGMAI EESTSPFAEL PFDGLVGLGF PDKNSKKNNI PTIVENIKER NILPRNLFGV YISRDSSTPG SISFGAADPK YTISGQKITW HRLTGSHYWE IKIKDIKING VSTNYCFGDC KAAIDTGSSV STAPSSFMRK ITKVIPMEGE CNRYLSSPRI TYVIEDIHGN EIEMHMDPQD YVIDEGGPSS FNAFSYYWGP QEHYCSIGYI PLDIPAPRGP LFVLGNNFIR KYYSIFDRDN LMVGFTLAKH SKYGPMFISL SSNSESVLKM ATFGITIILS LLLFIIY

NT Sequence

atggtgtttc aagtgttttt tattttaatg atgctgaaaa tatcagcttt ttgtgaagga ttatcaaaca agattaggtc aaatgctagg gcagagttag gaatggaggc tatactaagt aatggcttat ttaattcaaa tgctcaaaaa gttgagttaa aattaagtct atctgaggga ggaatgaaat attcagaagg caactatttt tcaaggtgga ttaccaagag taagtattca gagacaatgg actctcagga tcttagaaat taccaaaata gtcaatattt tggtaaaatt gaggtgggaa cgcctccaag agagttcgtt gtcatttttg atacaggaag ctccagtgta tggattccaa gtattgaatg taagcataaa ggttgtgagc cccacaacaa gtatgatcca aagttgagta cctcttatca aaaattgggg gatggatcat tggagacata tatccaatat ggtacgggtt cttgtgtgtt aaagtttggt aaagaagtca taaatattgg aaagctgaag atagaagatc agtctttcgg gatggctatt gaggaaagta catctccttt tgctgaatta ccattcgatg gtttggtagg acttggattt ccagataaga acagtaaaaa aaataatatc ccaacaattg ttgagaatat taaagaacga aacattttac caagaaatct ttttggagtt tacatttcca gagattcaag tactccaggt tcaatttctt ttggagctgc tgatcctaaa tacacaataa gtggtcaaaa aataacctgg catcgactta ctgggtctca ttactgggaa atcaagatca aagatataaa gattaatggg gttagcacga actattgctt cggggactgc aaggcagcta tagatactgg ttcatctgtc tccacagccc caagtagttt tatgagaaag atcaccaaag ttatacccat ggagggagaa tgtaatagat atctatcttc tcctagaata acttatgtga tagaagatat tcatggaaat gagatagaaa tgcatatgga tcctcaagac tatgtaattg atgaaggagg gccttcatca tttaatgctt ttagctatta ttggggcccg caagaacatt attgtagcat tggatatatc cctcttgata ttccagctcc tagaggtcca ctttttgtac ttggaaataa cttcattcga aagtactact caatatttga tagagataac ctaatggtgg gcttcacatt ggcaaaacat agtaaatatg ggcctatgtt catctcatta tcatccaatt cagaatctgt tcttaaaatg gctacatttg gaataaccat tatcctcagc ctgctactat ttattattta ttaa

Details for CrpaA.17789.b.A2.GE46268

HARVESTED ON:	7/17/2026
SEQUENCED ON:	data unavailable
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SFCEGLSNKI RSNARAELGM EAILSNGLFN SNAQKVELKL SLSEGGMKYS EGNYFSRWIT KSKYSETMDS QDLRNYQNSQ YFGKIEVGTP PREFVVIFDT GSSSVWIPSI ECKHKGCEPH NKYDPKLSTS YQKLGDGSLE TYIQYGTGSC VLKFGKEVIN IGKLKIEDQS FGMAIEESTS PFAELPFDGL VGLGFPDKNS KKNNIPTIVE NIKERNILPR NLFGVYISRD SSTPGSISFG AADPKYTISG QKITWHRLTG SHYWEIKIKD IKINGVSTNY CFGDCKAAID TGSSVSTAPS SFMRKITKVI PMEGECNRYL SSPRITYVIE DIHGNEIEMH MDPQDYVIDE GGPSSFNAFS YYWGPQEHYC SIGYIPLDIP APRGPLFVLG NNFIRKYYSI FDRDNLMVGF TLAKHSKYGP MFISLSSNSE S

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgtt ttgtgaagga ttatcaaaca agattaggtc aaatgctagg gcagagttag gaatggaggc tatactaagt aatggcttat ttaattcaaa tgctcaaaaa gttgagttaa aattaagtct atctgaggga ggaatgaaat attcagaagg caactatttt tcaaggtgga ttaccaagag taagtattca gagacaatgg actctcagga tcttagaaat taccaaaata gtcaatattt tggtaaaatt gaggtgggaa cgcctccaag agagttcgtt gtcatttttg atacaggaag ctccagtgta tggattccaa gtattgaatg taagcataaa ggttgtgagc cccacaacaa gtatgatcca aagttgagta cctcttatca aaaattgggg gatggatcat tggagacata tatccaatat ggtacgggtt cttgtgtgtt aaagtttggt aaagaagtca taaatattgg aaagctgaag atagaagatc agtctttcgg gatggctatt gaggaaagta catctccttt tgctgaatta ccattcgatg gtttggtagg acttggattt ccagataaga acagtaaaaa aaataatatc ccaacaattg ttgagaatat taaagaacga aacattttac caagaaatct ttttggagtt tacatttcca gagattcaag tactccaggt tcaatttctt ttggagctgc tgatcctaaa tacacaataa gtggtcaaaa aataacctgg catcgactta ctgggtctca ttactgggaa atcaagatca aagatataaa gattaatggg gttagcacga actattgctt cggggactgc aaggcagcta tagatactgg ttcatctgtc tccacagccc caagtagttt tatgagaaag atcaccaaag ttatacccat ggagggagaa tgtaatagat atctatcttc tcctagaata acttatgtga tagaagatat tcatggaaat gagatagaaa tgcatatgga tcctcaagac tatgtaattg atgaaggagg gccttcatca tttaatgctt ttagctatta ttggggcccg caagaacatt attgtagcat tggatatatc cctcttgata ttccagctcc tagaggtcca ctttttgtac ttggaaataa cttcattcga aagtactact caatatttga tagagataac ctaatggtgg gcttcacatt ggcaaaacat agtaaatatg ggcctatgtt catctcatta tcatccaatt cagaatctta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a