CrpaA.17369.b

vacuolar ATP synthase subunit b

CENTER ID: CrpaA.17369.b
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.17369.b.B1.GE35738 full length 1 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_1670
RefSeq: XP_627073.1
UniProt: Q5CW46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
RSYNSLFQSD SNYRKMVTSG QAYEANVAAV TRNYNIAPRM EYRTVSGVQG PLVILDNVKF PKYNEIVRIT LGDGSIREGQ VLEVKGSRAV VQVFEGTTGI DNQSCRVEFT GDVLCMPITE DMLGRTFNGS GKPIDNGPPI FAEDYIDING SPINPYCRVH PREMIQTGIS AIDVMNSVVR GQKIPLFSAA GLPHNDIGAQ ICRQASLVSG KDVMDHSDDN FAVVFAAMGV NMETARFFRR DFEENGSMER VCLFLNLAND PTIERILTPK LALTTAEYLA YEKDLHVFVV MTDMSSYADA LREVSAAREE VPGRRGYPGY MYTDLSTIYE RAGRIEGRNG SITQFPILTM PNDDITHPIP DLTGYITEGQ IFVDRALYNR QIYPPINVLP SLSRLMKSGI GTGLTREDHP SVSDQLYANY AIGQDTRAMV AVVGEEALSA DDLLYLEFTS KFESKFLSQG PYEKRDIFES LDLAWELLRT FPEDMLKKIK HDLLQKYYPR DNYIQHSK
NT Sequence
agaagttata atagtctttt tcagagtgat agtaattaca gaaagatggt aacttcaggc caggcttatg aggcaaatgt agcagcagtt acacgaaact ataatatcgc tcctagaatg gagtatagaa cagtttcagg agttcaaggt ccattagtaa ttttagataa tgtaaagttc ccaaaataca atgaaattgt aagaattact ttgggagatg gttctattag agaaggacaa gttttggagg tcaaaggaag tagagctgtt gtgcaagtat ttgagggtac tacaggcatt gacaatcaat catgtagagt tgaatttact ggggatgtat tgtgtatgcc aataacagaa gatatgttgg gtagaacatt taatggaagc ggaaaaccaa ttgataatgg ccctccaatt tttgctgaag attatattga tattaatggt agtccaatta atccatactg tagagttcat ccaagagaaa tgattcagac aggaatttct gcaattgatg ttatgaactc agttgttaga ggtcagaaga ttccattatt ttctgctgca ggacttccac ataatgatat tggtgcacaa atttgccgtc aagcttctct agtatccgga aaagatgtta tggatcattc tgatgataat tttgctgtag tatttgctgc tatgggagtt aatatggaga ctgcaagatt ctttagacgt gattttgagg aaaatggttc tatggaaaga gtatgcctct ttttgaattt agctaatgat ccaactattg aaagaatttt aactccaaaa ttggcattaa caacagcaga gtacttggct tatgagaagg atttacatgt atttgttgtt atgactgata tgagttctta tgcagatgct cttagagagg tttctgctgc gagagaggaa gttcctggta gaagaggtta tcctggttat atgtatacag atctttcaac tatttatgaa agagctggaa gaattgaggg tagaaatggt tcaattactc aattcccaat tttaacaatg ccaaatgatg atattaccca tccaattcca gatcttactg gttacattac agaaggacag atctttgttg atagagctct ctataacaga cagatttatc caccaattaa tgttttacca tcattgtctc gtcttatgaa gtctggtatt ggtactggtt taactagaga agatcatcca tcagtttctg atcaacttta tgctaattat gctattggcc aggatactag agctatggta gcagttgtag gagaggaagc tctttcagct gatgatttat tatatcttga gttcactagt aaattcgagt ccaagttcct ttctcaaggt ccatatgaga agagagatat ttttgagagt ttggatcttg cttgggaact tcttagaaca ttccctgaag atatgcttaa gaagattaag catgatttat tacaaaagta ttatccaaga gacaattata ttcaacattc taagtaa
Details for CrpaA.17369.b.B1.GE35738
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 58kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 53
Validated AA Sequence
MRSYNSLFQS DSNYRKMVTS GQAYEANVAA VTRNYNIAPR MEYRTVSGVQ GPLVILDNVK FPKYNEIVRI TLGDGSIREG QVLEVKGSRA VVQVFEGTTG IDNQSCRVEF TGDVLCMPIT EDMLGRTFNG SGKPIDNGPP IFAEDYIDIN GSPINPYCRV HPREMIQTGI SAIDVMNSVV RGQKXPLFSA AGLPHNDIGA QICRQASLVS GXDVMXXSXD NFAVVXAAMG VNMETARXFR RDFEENXSME RVXLFLNLAX DPTIEXILTP KLALTT
Validated NT Sequence
tggctcacca ccaccaccac catatgagaa gttataatag tctttttcag agtgatagta attacagaaa gatggtaact tcaggccagg cttatgaggc aaatgtagca gcagttacac gaaactataa tatcgctcct agaatggagt atagaacagt ttcaggagtt caaggtccat tagtaatttt agataatgta aagttcccaa aatacaatga aattgtaaga attactttgg gagatggttc tattagagaa ggacaagttt tggaggtcaa aggaagtaga gctgttgtgc aagtatttga gggtactaca ggcattgaca atcaatcatg tagagttgaa tttactgggg atgtattgtg tatgccaata acagaagata tgttgggtag aacatttaat ggaagcggaa aaccaattga taatggccct ccaatttttg ctgaagatta tattgatatt aatggtagtc caattaatcc atactgtaga gttcatccaa gagaaatgat tcagacagga atttctgcaa ttgatgttat gaactcagtt gttagaggtc agaagantcc attattttct gctgcaggac ttccacataa tgatattggt gcacaaattt gccgtcaagc ttctctagta tccggaanag atgttatgga ncantctgan gataattttg ctgtagtant tgctgctatg ggagttaata tggagactgc aagantcttt agacgtgatt ttgaggaaaa tgnntctatg gaaagagtan gnctcttttt gaatttagct natgatccaa ctattgaaan aattttaact ccaaaattgg cattaacaac agca
Expected Protein Sequence
MAHHHHHHMR SYNSLFQSDS NYRKMVTSGQ AYEANVAAVT RNYNIAPRME YRTVSGVQGP LVILDNVKFP KYNEIVRITL GDGSIREGQV LEVKGSRAVV QVFEGTTGID NQSCRVEFTG DVLCMPITED MLGRTFNGSG KPIDNGPPIF AEDYIDINGS PINPYCRVHP REMIQTGISA IDVMNSVVRG QKIPLFSAAG LPHNDIGAQI CRQASLVSGK DVMDHSDDNF AVVFAAMGVN METARFFRRD FEENGSMERV CLFLNLANDP TIERILTPKL ALTTAEYLAY EKDLHVFVVM TDMSSYADAL REVSAAREEV PGRRGYPGYM YTDLSTIYER AGRIEGRNGS ITQFPILTMP NDDITHPIPD LTGYITEGQI FVDRALYNRQ IYPPINVLPS LSRLMKSGIG TGLTREDHPS VSDQLYANYA IGQDTRAMVA VVGEEALSAD DLLYLEFTSK FESKFLSQGP YEKRDIFESL DLAWELLRTF PEDMLKKIKH DLLQKYYPRD NYIQHSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa gttataatag tctttttcag agtgatagta attacagaaa gatggtaact tcaggccagg cttatgaggc aaatgtagca gcagttacac gaaactataa tatcgctcct agaatggagt atagaacagt ttcaggagtt caaggtccat tagtaatttt agataatgta aagttcccaa aatacaatga aattgtaaga attactttgg gagatggttc tattagagaa ggacaagttt tggaggtcaa aggaagtaga gctgttgtgc aagtatttga gggtactaca ggcattgaca atcaatcatg tagagttgaa tttactgggg atgtattgtg tatgccaata acagaagata tgttgggtag aacatttaat ggaagcggaa aaccaattga taatggccct ccaatttttg ctgaagatta tattgatatt aatggtagtc caattaatcc atactgtaga gttcatccaa gagaaatgat tcagacagga atttctgcaa ttgatgttat gaactcagtt gttagaggtc agaagattcc attattttct gctgcaggac ttccacataa tgatattggt gcacaaattt gccgtcaagc ttctctagta tccggaaaag atgttatgga tcattctgat gataattttg ctgtagtatt tgctgctatg ggagttaata tggagactgc aagattcttt agacgtgatt ttgaggaaaa tggttctatg gaaagagtat gcctcttttt gaatttagct aatgatccaa ctattgaaag aattttaact ccaaaattgg cattaacaac agcagagtac ttggcttatg agaaggattt acatgtattt gttgttatga ctgatatgag ttcttatgca gatgctctta gagaggtttc tgctgcgaga gaggaagttc ctggtagaag aggttatcct ggttatatgt atacagatct ttcaactatt tatgaaagag ctggaagaat tgagggtaga aatggttcaa ttactcaatt cccaatttta acaatgccaa atgatgatat tacccatcca attccagatc ttactggtta cattacagaa ggacagatct ttgttgatag agctctctat aacagacaga tttatccacc aattaatgtt ttaccatcat tgtctcgtct tatgaagtct ggtattggta ctggtttaac tagagaagat catccatcag tttctgatca actttatgct aattatgcta ttggccagga tactagagct atggtagcag ttgtaggaga ggaagctctt tcagctgatg atttattata tcttgagttc actagtaaat tcgagtccaa gttcctttct caaggtccat atgagaagag agatattttt gagagtttgg atcttgcttg ggaacttctt agaacattcc ctgaagatat gcttaagaag attaagcatg atttattaca aaagtattat ccaagagaca attatattca acattctaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat