CrpaA.17369.a

ATP synthase beta chain, putative

CENTER ID:	CrpaA.17369.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.17369.a.B2.GE35737	mature protein		21	540

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd2_1360
OrthoMCL:	OG5_127099
RefSeq:	XP_001388200.1
UniProt:	A3FQ64

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFMIRGKLLS QSLPCFKFTR REFLPLFSAF FQKKAIRNLE YYNLNKRWKS YSKTGKNLSE GYVSQIMGSV VDVKFEGKLP ELLNALEVKG HQNKLVLEVA QHLSDNSVRA IAMDVTEGLS RGEKVIDTGS PICVPVGKAT LGRMVNVMGN AIDGCGEINA KVKKPIHRTA PEYMEQVMEP SLIVTGIKAI DLLTPFIKGG KIGLFGGAGV GKTVLIMELI NNIAKKYGGY SVYTGVGERI REGYDLYNEM LANGVNKKSI VGSRVDSKHK RQPIYDFLGS KTALVYGQMN ETPGARARVA LTGLTMAEYF RDSMMQDVLF FVDNIYRFTQ AGSEVSALLG LLPTEIGYQP TLATDLGKLQ ERITTTKNGS ITSIQALYIP SDDINDPAPV AAFTHLDSTI VLSRKMSEVG IFPSIDPLES RSKVLDRNII GEEHYKVSTE VLSILQRYKQ LQSKISTQGT HALSEEERLI VSRARKVQKF LTQPFFMSEA FTGKPGVFVN LIDTIKGFNA IVSGEMDNLP ETMFYMKGSL DSLGKPDSTC

NT Sequence

atgtttatga ttcgaggaaa attgttaagt caatctcttc catgtttcaa attcacacga agagaatttt tgccactatt ttcggcgttt ttccagaaaa aagcgatccg caacctagaa tattataact taaacaaaag atggaagtct tatagtaaaa ctggcaaaaa tctctcagaa ggctatgtat cacaaatcat gggttcagtt gtagatgtaa aattcgaggg taaactacca gaactgttaa acgctttgga agttaaaggg catcaaaaca aactagtgct tgaagtggcg caacacttaa gtgataattc agttagggca atcgcaatgg acgttacaga aggactctcc agaggagaga aggttataga cactggaagc ccaatatgcg ttccagtagg caaggccaca cttggtagaa tggtgaatgt tatggggaat gcaattgacg ggtgtggaga aatcaacgct aaagttaaaa aaccgattca tagaactgcc cctgaataca tggaacaagt aatggagcct tcattaattg ttactgggat taaggcaata gatctactta caccattcat aaaaggtggg aaaataggac tatttggtgg agcgggagtt ggcaagactg ttcttatcat ggaactaata aacaatattg caaaaaagta cggtggttac tctgtatata caggggttgg agaacgcatc agggaagggt acgatttata taatgaaatg ttagcaaatg gcgtcaataa aaagtcgatt gttggatcac gagttgacag caagcataag agacagccta tatacgattt tttaggttct aagacagcgc tggtatatgg tcagatgaat gaaactccag gagcgcgagc gcgagttgca cttacaggtc taacaatggc agaatatttt agagattcaa tgatgcaaga cgtattattt ttcgtagata atatttaccg atttacacag gcagggagtg aagtttctgc gcttttaggg ctattaccaa ctgaaatcgg gtatcaaccc actctggcta ctgatcttgg taaacttcaa gaaagaatca caaccacgaa aaatggttca ataacatcta ttcaggcact ttatattccg tctgacgaca ttaatgatcc agcacctgtt gcagccttca cccaccttga ttctacaata gttctctcta gaaaaatgtc agaagttggg atattcccat ccattgatcc attggaaagt aggtccaagg ttttagatcg aaatattata ggcgaagagc actacaaagt ttctactgaa gttctttcga ttttacaaag gtataagcag ttacagagca aaatttctac tcaaggtact catgcacttt ctgaagagga aaggttgatc gtctccaggg caagaaaagt ccaaaaattt cttactcagc cgttttttat gtcggaggct tttaccggaa agccaggggt ttttgtcaac ttaattgata caattaaagg ttttaatgct attgtcagcg gtgaaatgga taaccttcca gagacaatgt tttatatgaa aggtagtctt gatagtctag gaaagccgga ttctacttgt tag

Details for CrpaA.17369.a.B2.GE35737

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/6/2012
EXPECTED MW:	58kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

ttaagaagga ganataccat ggctcaccac caccaccacc atatgagaga atttttgcca ctattttcgg cgtttttcca gaaaaaagcg atccgcaacc tagaatatta taacttaaac aaaagatgga agtcttatag taaaactggc aaaaatctct cagaaggcta tgtatcacaa atcatgggtt cagttgtaga tgtaaaattc gagggtaaac taccagaact gttaaacgct ttggaagtta aagggcatca aaacaaacta gtgcttgaag tggcgcaaca cttaagtgat aattcagtta gggcaatcgc aatggacgtt acagaaggac tctccagagg agagaaggtt atagacactg gaagcccaat atgcgttcca gtaggcaagg ccacacttgg tagaatggtg aatgttatgg ggaatgcaat tgacgggtgt ggagaaatca acgctaaagt taaaaaaccg attcatagaa ctgcccctga atacatggaa caagtaatgg agccttcatt aattgttact gggattaagg caatagatct acttacacca ttcataaaag gtgggaaaat aggactattt ggtggagcgg gagttggcaa gactgttctt atcatggaac taataaacaa tattgcaaaa aagtacggtg gttactctgt atatacaggg gttggagaac gcatcangga agggtacgat ttatataatg aaatgttagc aaatggcgtc aataaaaagt cnattgttgg atcacgagtt gacagcaagc ataanagaca gcctatatac nattttttag gttctaanac agcgctngna tatggtcana ngaatgaaac tccnggagcn cgagcgcgag ttgcacttac nggtctaaca atggca

Expected Protein Sequence

MAHHHHHHMR EFLPLFSAFF QKKAIRNLEY YNLNKRWKSY SKTGKNLSEG YVSQIMGSVV DVKFEGKLPE LLNALEVKGH QNKLVLEVAQ HLSDNSVRAI AMDVTEGLSR GEKVIDTGSP ICVPVGKATL GRMVNVMGNA IDGCGEINAK VKKPIHRTAP EYMEQVMEPS LIVTGIKAID LLTPFIKGGK IGLFGGAGVG KTVLIMELIN NIAKKYGGYS VYTGVGERIR EGYDLYNEML ANGVNKKSIV GSRVDSKHKR QPIYDFLGSK TALVYGQMNE TPGARARVAL TGLTMAEYFR DSMMQDVLFF VDNIYRFTQA GSEVSALLGL LPTEIGYQPT LATDLGKLQE RITTTKNGSI TSIQALYIPS DDINDPAPVA AFTHLDSTIV LSRKMSEVGI FPSIDPLESR SKVLDRNIIG EEHYKVSTEV LSILQRYKQL QSKISTQGTH ALSEEERLIV SRARKVQKFL TQPFFMSEAF TGKPGVFVNL IDTIKGFNAI VSGEMDNLPE TMFYMKGSLD SLGKPDSTC

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagag aatttttgcc actattttcg gcgtttttcc agaaaaaagc gatccgcaac ctagaatatt ataacttaaa caaaagatgg aagtcttata gtaaaactgg caaaaatctc tcagaaggct atgtatcaca aatcatgggt tcagttgtag atgtaaaatt cgagggtaaa ctaccagaac tgttaaacgc tttggaagtt aaagggcatc aaaacaaact agtgcttgaa gtggcgcaac acttaagtga taattcagtt agggcaatcg caatggacgt tacagaagga ctctccagag gagagaaggt tatagacact ggaagcccaa tatgcgttcc agtaggcaag gccacacttg gtagaatggt gaatgttatg gggaatgcaa ttgacgggtg tggagaaatc aacgctaaag ttaaaaaacc gattcataga actgcccctg aatacatgga acaagtaatg gagccttcat taattgttac tgggattaag gcaatagatc tacttacacc attcataaaa ggtgggaaaa taggactatt tggtggagcg ggagttggca agactgttct tatcatggaa ctaataaaca atattgcaaa aaagtacggt ggttactctg tatatacagg ggttggagaa cgcatcaggg aagggtacga tttatataat gaaatgttag caaatggcgt caataaaaag tcgattgttg gatcacgagt tgacagcaag cataagagac agcctatata cgatttttta ggttctaaga cagcgctggt atatggtcag atgaatgaaa ctccaggagc gcgagcgcga gttgcactta caggtctaac aatggcagaa tattttagag attcaatgat gcaagacgta ttatttttcg tagataatat ttaccgattt acacaggcag ggagtgaagt ttctgcgctt ttagggctat taccaactga aatcgggtat caacccactc tggctactga tcttggtaaa cttcaagaaa gaatcacaac cacgaaaaat ggttcaataa catctattca ggcactttat attccgtctg acgacattaa tgatccagca cctgttgcag ccttcaccca ccttgattct acaatagttc tctctagaaa aatgtcagaa gttgggatat tcccatccat tgatccattg gaaagtaggt ccaaggtttt agatcgaaat attataggcg aagagcacta caaagtttct actgaagttc tttcgatttt acaaaggtat aagcagttac agagcaaaat ttctactcaa ggtactcatg cactttctga agaggaaagg ttgatcgtct ccagggcaag aaaagtccaa aaatttctta ctcagccgtt ttttatgtcg gaggctttta ccggaaagcc aggggttttt gtcaacttaa ttgatacaat taaaggtttt aatgctattg tcagcggtga aatggataac cttccagaga caatgtttta tatgaaaggt agtcttgata gtctaggaaa gccggattct acttgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t