CrpaA.17190.a

p97; CDC48 like AAA ATPase ortholog

CENTER ID: CrpaA.17190.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.17190.a.B3.GE41006 structured domain 40 791
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd1_330
RefSeq: XP_627893.1
UniProt: Q5CT24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVDGENQIP KSEEVQMTNG DSSANNTSPG QIEKKRAPNR LLVDDAINDD NSVVCLSPAK MEELKLFRGD TILLKGKKRR DTICIVLVDP DLEEGKIRMN KVVRKNLRVK LGDTVSVLEC GDVPYGKRIH VLPFDDCLEG ITGNLFETYL KPYFLEAYRP VKKGDAFLVR GGFRPIEFKV VGVDPEEYCI VAPDTVIHCE GDPIKREDEE KMDDIGYDDI GGCRKQMAQI REMIELPLRH PGLFKALGVK PPRGVLLYGP PGSGKTLIAK AVANETGAFF FLINGPEVMS KMAGEAEGNL RRAFEEAEKN SPAIIFIDEI DSIAPKREKT NGEVERRVVS QLLTLMDGLK GRGQVVVIAA TNRPNSIDPA LRRFGRFDRE IDIGVPDDNG RLEIIRIHTR NMKLAKDVKI DDIAANTHGF VGADLAQLCT EAALCCIREK MDVIDMEDET IDAVILDSMA VSQDHFNSAL GVCNPSSLRE TVVEVPNIKW DDIGGLEEVK RNLQEMILYP IEHPEKFERF GMSPSRGVLF YGPPGCGKTL LAKAVASECS ANFISVKGPE LLTLWFGESE ANVREVFDKA RAAAPCVLFF DELDSIGTQR GSSMGDAGGA GDRVMNQLLT EIDGVGVKKN LFFIGATNRP EILDEALLRP GRLDQLIYIP LPDLPARVSV LQAILRKSPL SKNVPISFIA QKTEGFSGAD LAELCQRAAK AAIRDAIAAE ELKKASGDDS AMKIEDEVDS HIYEIGRKHF EEAFAGARRS VSITDLAKYD QFRMKFDPVY VTQSGGEGFT IDWPDSTHAQ YSAPIDDDAD DLYS
NT Sequence
atgaatgttg acggggaaaa tcaaattcca aagtcagagg aagttcaaat gactaatggt gattcctctg ctaataatac gtcaccaggt caaattgaaa aaaagagagc tccaaataga ttgctggttg acgatgctat taatgatgat aattctgttg tatgtttaag tccagcaaag atggaggagt tgaaattatt tagaggtgat acaattttat taaagggtaa gaaacgtaga gatacaatat gtatagtatt ggttgaccct gatttggagg aaggtaaaat tcgtatgaac aaggttgtca ggaagaactt gagagttaag cttggagata cagtttctgt tcttgagtgt ggagatgttc catatggtaa gaggattcat gttttgccat ttgatgactg tttggaagga attactggta atctttttga gacatatttg aaaccgtatt tcctagaggc ttacagaccc gtcaaaaagg gagatgcttt tttggtaaga ggcggattcc gtccaatcga gttcaaggtt gttggagtag atccagaaga gtactgcatt gtagctccag atactgtaat tcactgtgag ggtgatccaa tcaagagaga agatgaggaa aaaatggatg atattggata tgacgatatt ggaggctgca gaaagcaaat ggctcaaatc agagaaatga ttgagcttcc attacgtcat ccagggctat tcaaagcttt aggagttaaa cctccgagag gagtcctatt atatggacct cctggttctg gtaagactct tattgcaaag gcagtagcaa atgaaactgg agcattcttc ttcctgatta atggaccaga agttatgtca aaaatggcag gagaagcaga gggaaattta cgtagagctt tcgaggaggc agaaaagaac tctcctgcga ttattttcat tgatgaaatt gattccattg ctcctaagag agagaagact aatggagagg tcgaacgtcg tgttgtatct cagttattaa ctcttatgga tggattgaaa gggaggggcc aagttgttgt tattgcagct acaaatagac caaattctat tgatccagca ttgagaagat ttggcagatt cgacagagaa attgatattg gagtcccaga tgataatggt agacttgaaa ttatcagaat ccatacaaga aatatgaagc tagctaagga tgtcaaaatt gatgatattg cagctaatac ccatggtttt gttggagcag atttggccca gctatgtact gaggcggctc tctgttgtat tcgtgagaaa atggatgtta ttgatatgga agatgaaaca attgacgcag ttatcttgga ttctatggcc gtcagccagg atcacttcaa ctctgcattg ggcgtttgca acccatcttc tttgagagaa actgttgtag aggttccaaa cattaaatgg gatgatattg gtggtcttga agaagtcaaa cgcaacttac aagaaatgat actctaccct attgagcatc ctgagaagtt cgagcgtttt ggaatgagtc cttctcgtgg agtcttattt tatggtccac ctggttgtgg taagacttta cttgcaaaag ctgttgcctc tgaatgctct gcaaacttta tttctgtcaa aggacccgaa cttcttacct tatggtttgg tgagagtgag gccaacgtta gggaggtatt tgataaggcc agagctgctg ctccttgtgt actattcttc gatgagttag acagtattgg cactcaaaga ggaagcagca tgggagatgc tggtggtgct ggagacagag ttatgaatca acttcttact gaaatcgatg gtgttggtgt taagaagaat ctcttcttca ttggagctac aaatagacca gagatccttg atgaggcttt attgcgtcca ggaaggcttg accagttaat ttacatccct ctcccagatc ttcctgccag agtctctgtt ctacaagcta ttcttagaaa atctcctctt tcaaagaacg ttcctatatc ttttatcgct cagaaaactg aaggattctc aggcgctgac cttgcagagc tttgccaaag agcagcaaag gctgcaatta gagatgcaat tgctgctgaa gagctaaaga aggcatctgg ggatgatagt gctatgaaaa ttgaggatga agtagatagt catatttacg aaatcggaag aaaacatttt gaggaagcat ttgctggagc gagaagatct gtaagcatta ccgaccttgc caaatatgat cagtttagaa tgaagtttga ccctgtgtat gtcactcaat ccggaggaga aggatttaca attgactggc cagattcgac ccatgcccaa tactctgctc ccattgatga cgatgctgat gatttatatt cataa
Details for CrpaA.17190.a.B3.GE41006
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 84kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRL LVDDAINDDN SVVCLSPAKM EELKLFRGDT ILLKGKKRRD TICIVLVDPD LEEGKIRMNK VVRKNLRVKL GDTVSVLECG DVPYGKRIHV LPFDDCLEGI TGNLFETYLK PYFLEAYRPV KKGDAFLVRG GFRPIEFKVV GVDPEEYCIV APDTVIHCEG DPIKREDEEK MDDIGYDDIG GCRKQMAQIR EMIELPLRHP GLFKALGVKP PRGVLLYGPP GSGKTLIAKA VANETGAFFF LINGPEVMSK MAGEAEGNLR RAFEEAEKNS PAIIFIDEID SIAPKREKTN GEVERRVVSQ LLTLMDGLKG RGQVVVIAAT NRPNSIDPAL RRFGRFDREI DIGVPDDNGR LEIIRIHTRN MKLAKDVKID DIAANTHGFV GADLAQLCTE AALCCIREKM DVIDMEDETI DAVILDSMAV SQDHFNSALG VCNPSSLRET VVEVPNIKWD DIGGLEEVKR NLQEMILYPI EHPEKFERFG MSPSRGVLFY GPPGCGKTLL AKAVASECSA NFISVKGPEL LTLWFGESEA NVREVFDKAR AAAPCVLFFD ELDSIGTQRG SSMGDAGGAG DRVMNQLLTE IDGVGVKKNL FFIGATNRPE ILDEALLRPG RLDQLIYIPL PDLPARVSVL QAILRKSPLS KNVPISFIAQ KTEGFSGADL AELCQRAAKA AIRDAIAAEE LKKASGDDSA MKIEDEVDSH IYEIGRKHFE EAFAGARRSV SITDLAKYDQ FRMKFDPVYV TQSGGEGFTI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagattgc tggttgacga tgctattaat gatgataatt ctgttgtatg tttaagtcca gcaaagatgg aggagttgaa attatttaga ggtgatacaa ttttattaaa gggtaagaaa cgtagagata caatatgtat agtattggtt gaccctgatt tggaggaagg taaaattcgt atgaacaagg ttgtcaggaa gaacttgaga gttaagcttg gagatacagt ttctgttctt gagtgtggag atgttccata tggtaagagg attcatgttt tgccatttga tgactgtttg gaaggaatta ctggtaatct ttttgagaca tatttgaaac cgtatttcct agaggcttac agacccgtca aaaagggaga tgcttttttg gtaagaggcg gattccgtcc aatcgagttc aaggttgttg gagtagatcc agaagagtac tgcattgtag ctccagatac tgtaattcac tgtgagggtg atccaatcaa gagagaagat gaggaaaaaa tggatgatat tggatatgac gatattggag gctgcagaaa gcaaatggct caaatcagag aaatgattga gcttccatta cgtcatccag ggctattcaa agctttagga gttaaacctc cgagaggagt cctattatat ggacctcctg gttctggtaa gactcttatt gcaaaggcag tagcaaatga aactggagca ttcttcttcc tgattaatgg accagaagtt atgtcaaaaa tggcaggaga agcagaggga aatttacgta gagctttcga ggaggcagaa aagaactctc ctgcgattat tttcattgat gaaattgatt ccattgctcc taagagagag aagactaatg gagaggtcga acgtcgtgtt gtatctcagt tattaactct tatggatgga ttgaaaggga ggggccaagt tgttgttatt gcagctacaa atagaccaaa ttctattgat ccagcattga gaagatttgg cagattcgac agagaaattg atattggagt cccagatgat aatggtagac ttgaaattat cagaatccat acaagaaata tgaagctagc taaggatgtc aaaattgatg atattgcagc taatacccat ggttttgttg gagcagattt ggcccagcta tgtactgagg cggctctctg ttgtattcgt gagaaaatgg atgttattga tatggaagat gaaacaattg acgcagttat cttggattct atggccgtca gccaggatca cttcaactct gcattgggcg tttgcaaccc atcttctttg agagaaactg ttgtagaggt tccaaacatt aaatgggatg atattggtgg tcttgaagaa gtcaaacgca acttacaaga aatgatactc taccctattg agcatcctga gaagttcgag cgttttggaa tgagtccttc tcgtggagtc ttattttatg gtccacctgg ttgtggtaag actttacttg caaaagctgt tgcctctgaa tgctctgcaa actttatttc tgtcaaagga cccgaacttc ttaccttatg gtttggtgag agtgaggcca acgttaggga ggtatttgat aaggccagag ctgctgctcc ttgtgtacta ttcttcgatg agttagacag tattggcact caaagaggaa gcagcatggg agatgctggt ggtgctggag acagagttat gaatcaactt cttactgaaa tcgatggtgt tggtgttaag aagaatctct tcttcattgg agctacaaat agaccagaga tccttgatga ggctttattg cgtccaggaa ggcttgacca gttaatttac atccctctcc cagatcttcc tgccagagtc tctgttctac aagctattct tagaaaatct cctctttcaa agaacgttcc tatatctttt atcgctcaga aaactgaagg attctcaggc gctgaccttg cagagctttg ccaaagagca gcaaaggctg caattagaga tgcaattgct gctgaagagc taaagaaggc atctggggat gatagtgcta tgaaaattga ggatgaagta gatagtcata tttacgaaat cggaagaaaa cattttgagg aagcatttgc tggagcgaga agatctgtaa gcattaccga ccttgccaaa tatgatcagt ttagaatgaa gtttgaccct gtgtatgtca ctcaatccgg aggagaagga tttacaattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat