CrpaA.01637.a

Serine hydroxymethyltransferase

CENTER ID: CrpaA.01637.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01637.a.A1.GE26529 Full length( CrpaA.01637.a ) 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_2610
RefSeq: XP_627161.1
UniProt: Q5CVV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVFLQEKSLK ELDPIMYELI NEEYDRQING LEMIASENFV SKGVLDSLSS TFSMFNNDKN MELNSTSAQE LLELTNERAL KAYGLDPEVW GANVKPHSGS PANFAVLNAV LKPNDRIMGL SLQHGGHLTH GHYTNLKRVN CSSHYFESLP YVTDLEGVID YDKLEENAIL FRPKMIIAGA SGYPRMINFK RFRDICDKVK AYLMVDIAHY SGLVVAGKYP SPKDYADFIT TTSHKTLRGP RSAIIFYRKD VESKIRVKID ESVSKEIQSS IHFNQVAALC FQLKQVVSAS WVKYASRVLE SSQLLCKLLE ESGIKILTNG TDSHKILIDT RSLNISGAKA EKALEVCEIS TSRSSLPCDG RTMNCSGVRL GTAALASRGM ELDDFKFVSR IIVEVLTTAR DIQKDGETLA EFVSNIKASP AIENIRQTVR KFASSFEFVS IVDKY
NT Sequence
ATGGTATTTT TACAGGAAAA ATCATTAAAA GAGCTGGATC CCATTATGTA TGAGCTAATC AATGAAGAAT ATGATAGACA GATTAATGGA CTAGAAATGA TAGCATCCGA AAACTTTGTA TCGAAGGGAG TCTTGGACAG CTTGTCAAGT ACATTCTCAA TGTTTAATAA TGATAAAAAT ATGGAATTAA ACTCAACTTC TGCACAAGAA CTATTAGAGC TCACAAATGA AAGAGCCTTA AAAGCATATG GACTAGATCC TGAAGTATGG GGTGCTAATG TAAAGCCTCA TAGTGGCTCT CCTGCAAATT TTGCAGTATT AAATGCAGTA TTAAAACCTA ATGATAGAAT AATGGGATTG TCATTACAAC ATGGAGGCCA CCTGACTCAC GGACACTATA CAAATTTGAA GAGAGTTAAC TGTTCATCTC ACTATTTTGA GTCACTCCCT TATGTAACTG ATTTAGAAGG AGTGATAGAT TACGATAAGT TGGAAGAAAA TGCAATACTA TTTAGACCAA AGATGATAAT AGCAGGAGCT AGTGGGTATC CTAGAATGAT CAACTTTAAG AGGTTTAGGG ATATCTGCGA CAAAGTGAAG GCCTACCTGA TGGTAGATAT CGCTCACTAC TCTGGTTTGG TGGTTGCAGG GAAGTACCCG TCACCCAAAG ACTACGCAGA TTTCATTACA ACAACGTCTC ACAAGACATT AAGAGGACCA AGGTCAGCAA TAATATTTTA CAGAAAGGAC GTAGAGTCCA AGATCAGAGT TAAGATTGAT GAGTCAGTTT CTAAAGAAAT TCAGTCTAGT ATTCATTTCA ACCAAGTTGC AGCCCTTTGT TTCCAACTGA AACAAGTAGT TTCTGCTTCT TGGGTTAAAT ATGCAAGTAG AGTGCTAGAA AGCTCTCAAT TGCTTTGCAA ATTGCTCGAG GAGTCTGGAA TCAAGATTCT TACAAATGGA ACTGATTCCC ACAAAATTCT AATAGATACA AGATCATTGA ACATTTCTGG GGCTAAGGCT GAGAAAGCAC TAGAAGTATG CGAAATTTCA ACAAGTAGAA GTTCTTTACC TTGTGATGGG AGAACTATGA ATTGCAGTGG CGTCAGACTT GGAACTGCAG CCCTTGCGAG CAGAGGGATG GAGTTGGATG ATTTCAAATT CGTTTCAAGG ATAATTGTAG AAGTTTTAAC TACTGCTCGA GATATTCAAA AGGATGGGGA GACTCTTGCA GAGTTTGTTT CTAATATTAA GGCCTCTCCA GCAATAGAGA ATATCAGGCA AACAGTGCGA AAGTTTGCCT CATCGTTTGA ATTTGTTTCT ATCGTAGATA AGTAT
Details for CrpaA.01637.a.A1.GE26529
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 90
Validated AA Sequence
MVFLQEKSLK ELDPIMYELI NEEYDRQING LEMIASENFV SKGVLDSLSS TFSMFNNNKN MELNSTSAQE LLELTNERAL KAYGLDPEVW GANVKPHSGS PANFAVLNAV LKPNDRIMGL SLQHGGHLTH GHYTNLKRVN CSSHYFESLP YVTDLEGVID YDKLEENAIL FRPKMIIAGA SGYPRMINFK RFRXICDKVK AYLMVDIAHY SGLVVAGKYP SXKDYADFIT TTSHKTLRGP RSAIIFYRKD VESKIRVKID ESVSKEIQSS IHFNQVAALC FQLKQVVSAS WVKYASRVLE SSQLLCKLLE ESGIKILTNG TDSHKIXIDT RSLNISGAKA EKALEVCEIS TSRSSLPCDG RTMNCSGVRL GTAALASRGM ELDDFKFVSR IIVEVLTTAR DIQKDGETLA EFVXNIKASP
Validated NT Sequence
gctggagagg ccttaatatt anaaacaaac tctgcaagag tctccccatc cttttgaata tctcgagcag tagttaaaac ttctacaatt atccttgaaa cgaatttgaa atcatccaac tccatccctc tgctcgcaag ggctgcagtt ccaagtctga cgccactgca attcatagtt ctcccatcac aaggtaaaga acttctactt gttgaaattt cgcatacttc tagtgctttc tcagccttag ccccagaaat gttcaatgat cttgtatcta ttanaatttt gtgggaatca gttccatttg taagaatctt gattccagac tcctcgagca atttgcaaag caattgagag ctttctagca ctctacttgc atatttaacc caagaagcag aaactacttg tttcagttgg aaacaaaggg ctgcaacttg gttgaaatga atactagact gaatttcttt agaaactgac tcatcaatct taactctgat cttggactct acgtcctttc tgtaaaatat tattgctgac cttggtcctc ttaatgtctt gtgagacgtt gttgtaatga aatctgcgta gtctttggnt gacgggtact tccctgcaac caccaaacca gagtagtgag cgatatctac catcaggtag gccttcactt tgtcgcagat ntccctaaac ctcttaaagt tgatcattct aggataccca ctagctcctg ctattatcat ctttggtcta aatagtattg cattttcttc caacttatcg taatctatca ctccttctaa atcagttaca taagggagtg actcaaaata gtgagatgaa cagttaactc tcttcaaatt tgtatagtgt ccgtgagtca ggtggcctcc atgttgtaat gacaatccca ttattctatc attaggtttt aatactgcat ttaatactgc aaaatttgca ggagagccac tatgaggctt tacattagca ccccatactt caggatctag tccatatgct tttaaggctc tttcatttgt gagctctaat agttcttgtg cagaagttga gtttaattcc atatttttat tattattaaa cattgagaat gtacttgaca agctgtccaa gactcccttc gatacaaagt tttcggatgc tatcatttct agtccattaa tctgtctatc atattcttca ttgattagct catacataat gggatccagc tcttttaatg atttttcctg taaaaatacc atcgaaccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFLQEKSL KELDPIMYEL INEEYDRQIN GLEMIASENF VSKGVLDSLS STFSMFNNDK NMELNSTSAQ ELLELTNERA LKAYGLDPEV WGANVKPHSG SPANFAVLNA VLKPNDRIMG LSLQHGGHLT HGHYTNLKRV NCSSHYFESL PYVTDLEGVI DYDKLEENAI LFRPKMIIAG ASGYPRMINF KRFRDICDKV KAYLMVDIAH YSGLVVAGKY PSPKDYADFI TTTSHKTLRG PRSAIIFYRK DVESKIRVKI DESVSKEIQS SIHFNQVAAL CFQLKQVVSA SWVKYASRVL ESSQLLCKLL EESGIKILTN GTDSHKILID TRSLNISGAK AEKALEVCEI STSRSSLPCD GRTMNCSGVR LGTAALASRG MELDDFKFVS RIIVEVLTTA RDIQKDGETL AEFVSNIKAS PAIENIRQTV RKFASSFEFV SIVDKY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtattttta caggaaaaat cattaaaaga gctggatccc attatgtatg agctaatcaa tgaagaatat gatagacaga ttaatggact agaaatgata gcatccgaaa actttgtatc gaagggagtc ttggacagct tgtcaagtac attctcaatg tttaataatg ataaaaatat ggaattaaac tcaacttctg cacaagaact attagagctc acaaatgaaa gagccttaaa agcatatgga ctagatcctg aagtatgggg tgctaatgta aagcctcata gtggctctcc tgcaaatttt gcagtattaa atgcagtatt aaaacctaat gatagaataa tgggattgtc attacaacat ggaggccacc tgactcacgg acactataca aatttgaaga gagttaactg ttcatctcac tattttgagt cactccctta tgtaactgat ttagaaggag tgatagatta cgataagttg gaagaaaatg caatactatt tagaccaaag atgataatag caggagctag tgggtatcct agaatgatca actttaagag gtttagggat atctgcgaca aagtgaaggc ctacctgatg gtagatatcg ctcactactc tggtttggtg gttgcaggga agtacccgtc acccaaagac tacgcagatt tcattacaac aacgtctcac aagacattaa gaggaccaag gtcagcaata atattttaca gaaaggacgt agagtccaag atcagagtta agattgatga gtcagtttct aaagaaattc agtctagtat tcatttcaac caagttgcag ccctttgttt ccaactgaaa caagtagttt ctgcttcttg ggttaaatat gcaagtagag tgctagaaag ctctcaattg ctttgcaaat tgctcgagga gtctggaatc aagattctta caaatggaac tgattcccac aaaattctaa tagatacaag atcattgaac atttctgggg ctaaggctga gaaagcacta gaagtatgcg aaatttcaac aagtagaagt tctttacctt gtgatgggag aactatgaat tgcagtggcg tcagacttgg aactgcagcc cttgcgagca gagggatgga gttggatgat ttcaaattcg tttcaaggat aattgtagaa gttttaacta ctgctcgaga tattcaaaag gatggggaga ctcttgcaga gtttgtttct aatattaagg cctctccagc aatagagaat atcaggcaaa cagtgcgaaa gtttgcctca tcgtttgaat ttgtttctat cgtagataag tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa