CrpaA.01586.a

ATP-dependent RNA helicase, putative

CENTER ID: CrpaA.01586.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01586.a.A1.GE26564 Full length( CrpaA.01586.a ) 1 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01586.a.A1.PW26609 Full length( CrpaA.01586.a ) 1 406 unavailable
CrpaA.01586.a.A1.PW27275 Full length( CrpaA.01586.a ) 1 406
External Resources
RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_1820
OrthoMCL: OG5_127804
RefSeq: XP_001388425.1
UniProt: A3FPY4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTERLSNDM KGLNIPPKDP RFKTDDVTKT KGSDFEDYFL KRELLMGIYE KGFERPSPIQ EESIPVALAG KDILARAKNG TGKTAAFVIP LLEKINTKKN IIQGLILVPT RELALQTSSI VKQLGKHINV QCMVSTGGTS LRDDILRLNN PVHVLVGTPG RILDLSNKKV CNLSGCFMFI MDEADKLLSP EFQPIIEELI EFLPKERQIL LYSATFPVTV KGFKDKYLAN AHEINLMDEL TLKGVTQYYA FVEEKQKLHC LNTLFSKLQI NQAIIFCNSV TRVELLAKKI TELGSSCFYI HARMLQSHRN RVFHDFRNGA CRCLVSSDLI TRGIDIQSVN VVINFDFPKY SETYLHRIGR SGRFGHLGLA INLITYEDRY NLYRIEKELA TEISPIPAQI DSALYT
NT Sequence
ATGAAGACTG AAAGATTAAG TAATGATATG AAGGGCTTGA ACATCCCTCC TAAAGATCCT AGGTTCAAGA CAGACGATGT AACTAAAACA AAGGGTAGTG ATTTTGAGGA CTACTTTTTA AAAAGAGAAC TTTTGATGGG AATTTATGAA AAAGGATTTG AGAGACCATC ACCAATTCAA GAAGAAAGTA TACCAGTGGC TCTTGCAGGC AAAGATATCC TAGCAAGAGC AAAGAATGGA ACAGGTAAGA CTGCAGCATT TGTTATACCA TTATTAGAGA AAATCAATAC TAAAAAGAAC ATTATTCAAG GCTTGATTCT TGTCCCAACA AGAGAACTTG CTTTACAAAC TAGCAGTATT GTGAAGCAGC TTGGTAAGCA TATCAATGTC CAGTGTATGG TTAGTACTGG TGGTACATCT TTAAGAGATG ATATATTACG TTTAAACAAC CCAGTTCATG TATTGGTAGG AACTCCTGGT CGTATTTTGG ATCTGTCTAA TAAGAAGGTG TGTAACTTGA GTGGTTGTTT TATGTTTATT ATGGATGAGG CCGATAAATT ATTAAGTCCA GAATTTCAGC CAATTATTGA GGAACTCATA GAGTTTTTAC CAAAAGAAAG GCAAATTCTC TTATATTCTG CTACTTTCCC CGTAACTGTT AAAGGTTTTA AGGATAAATA TCTTGCAAAT GCTCATGAAA TTAATCTAAT GGACGAGCTC ACTTTAAAGG GTGTTACACA ATATTATGCT TTTGTTGAAG AGAAGCAGAA ATTACATTGT CTTAATACTT TATTTAGTAA ACTACAAATT AACCAAGCCA TTATATTTTG TAATTCTGTT ACAAGAGTTG AACTTTTAGC AAAGAAAATT ACTGAGCTTG GAAGCTCATG CTTTTATATT CACGCAAGAA TGCTCCAGTC TCACAGAAAT AGAGTATTTC ATGACTTCAG AAACGGTGCA TGTAGGTGTC TTGTATCATC TGATCTTATC ACAAGAGGTA TTGATATCCA ATCTGTTAAT GTCGTTATTA ATTTTGACTT CCCTAAATAT TCCGAAACAT ACCTTCACAG AATTGGTAGA TCTGGGAGAT TTGGGCATCT TGGGCTCGCA ATCAACCTTA TAACTTATGA AGATCGCTAT AACTTATATA GAATTGAAAA AGAATTGGCC ACAGAGATTT CACCAATTCC CGCCCAAATT GATTCTGCCT TATATACT
Details for CrpaA.01586.a.A1.GE26564
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSAL
Validated NT Sequence
ataaggcaga atcaatttgg gcgggaattg gtgaaatctc tgtggccaat tctttttcaa ttctatataa gttatagcga tcttcataag ttataaggtt gattgcgagc ccaagatgcc caaatctccc agatctacca attctgtgaa ggtatgtttc ggaatattta gggaagtcaa aattaataac gacattaaca gattggatat caatacctct tgtgataaga tcagatgata caagacacct acatgcaccg tttctgaagt catgaaatac tctatttctg tgagactgga gcattcttgc gtgaatataa aagcatgagc ttccaagctc agtaattttc tttgctaaaa gttcaactct tgtaacagaa ttacaaaata taatggcttg gttaatttgt agtttactaa ataaagtatt aagacaatgt aatttctgct tctcttcaac aaaagcataa tattgtgtaa caccctttaa agtgagctcg tccattagat taatttcatg agcatttgca agatatttat ccttaaaacc tttaacagtt acggggaaag tagcagaata taagagaatt tgcctttctt ttggtaaaaa ctctatgagt tcctcaataa ttggctgaaa ttctggactt aataatttat cggcctcatc cataataaac ataaaacaac cactcaagtt acacaccttc ttattagaca gatccaaaat acgaccagga gttcctacca atacatgaac tgggttgttt aaacgtaata tatcatctct taaagatgta ccaccagtac taaccataca ctggacattg atatgcttac caagctgctt cacaatactg ctagtttgta aagcaagttc tcttgttggg acaagaatca agccttgaat aatgttcttt ttagtattga ttttctctaa taatggtata acaaatgctg cagtcttacc tgttccattc tttgctcttg ctaggatatc tttgcctgca agagccactg gtatactttc ttcttgaatt ggtgatggtc tctcaaatcc tttttcataa attcccatca aaagttctct ttttaaaaag tagtcctcaa aatcactacc ctttgtttta gttacatcgt ctgtcttgaa cctaggatct ttagggggga tgttcaagcc cttcatatca ttacttaatc tttcagtctt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSALYT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagactgaa agattaagta atgatatgaa gggcttgaac atccctccta aagatcctag gttcaagaca gacgatgtaa ctaaaacaaa gggtagtgat tttgaggact actttttaaa aagagaactt ttgatgggaa tttatgaaaa aggatttgag agaccatcac caattcaaga agaaagtata ccagtggctc ttgcaggcaa agatatccta gcaagagcaa agaatggaac aggtaagact gcagcatttg ttataccatt attagagaaa atcaatacta aaaagaacat tattcaaggc ttgattcttg tcccaacaag agaacttgct ttacaaacta gcagtattgt gaagcagctt ggtaagcata tcaatgtcca gtgtatggtt agtactggtg gtacatcttt aagagatgat atattacgtt taaacaaccc agttcatgta ttggtaggaa ctcctggtcg tattttggat ctgtctaata agaaggtgtg taacttgagt ggttgtttta tgtttattat ggatgaggcc gataaattat taagtccaga atttcagcca attattgagg aactcataga gtttttacca aaagaaaggc aaattctctt atattctgct actttccccg taactgttaa aggttttaag gataaatatc ttgcaaatgc tcatgaaatt aatctaatgg acgagctcac tttaaagggt gttacacaat attatgcttt tgttgaagag aagcagaaat tacattgtct taatacttta tttagtaaac tacaaattaa ccaagccatt atattttgta attctgttac aagagttgaa cttttagcaa agaaaattac tgagcttgga agctcatgct tttatattca cgcaagaatg ctccagtctc acagaaatag agtatttcat gacttcagaa acggtgcatg taggtgtctt gtatcatctg atcttatcac aagaggtatt gatatccaat ctgttaatgt cgttattaat tttgacttcc ctaaatattc cgaaacatac cttcacagaa ttggtagatc tgggagattt gggcatcttg ggctcgcaat caaccttata acttatgaag atcgctataa cttatataga attgaaaaag aattggccac agagatttca ccaattcccg cccaaattga ttctgcctta tatactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for CrpaA.01586.a.A1.PW26609
PURIFICATION DATe: 6/23/2009
CONCENTRATION: 1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSAL
Validated NT Sequence
ataaggcaga atcaatttgg gcgggaattg gtgaaatctc tgtggccaat tctttttcaa ttctatataa gttatagcga tcttcataag ttataaggtt gattgcgagc ccaagatgcc caaatctccc agatctacca attctgtgaa ggtatgtttc ggaatattta gggaagtcaa aattaataac gacattaaca gattggatat caatacctct tgtgataaga tcagatgata caagacacct acatgcaccg tttctgaagt catgaaatac tctatttctg tgagactgga gcattcttgc gtgaatataa aagcatgagc ttccaagctc agtaattttc tttgctaaaa gttcaactct tgtaacagaa ttacaaaata taatggcttg gttaatttgt agtttactaa ataaagtatt aagacaatgt aatttctgct tctcttcaac aaaagcataa tattgtgtaa caccctttaa agtgagctcg tccattagat taatttcatg agcatttgca agatatttat ccttaaaacc tttaacagtt acggggaaag tagcagaata taagagaatt tgcctttctt ttggtaaaaa ctctatgagt tcctcaataa ttggctgaaa ttctggactt aataatttat cggcctcatc cataataaac ataaaacaac cactcaagtt acacaccttc ttattagaca gatccaaaat acgaccagga gttcctacca atacatgaac tgggttgttt aaacgtaata tatcatctct taaagatgta ccaccagtac taaccataca ctggacattg atatgcttac caagctgctt cacaatactg ctagtttgta aagcaagttc tcttgttggg acaagaatca agccttgaat aatgttcttt ttagtattga ttttctctaa taatggtata acaaatgctg cagtcttacc tgttccattc tttgctcttg ctaggatatc tttgcctgca agagccactg gtatactttc ttcttgaatt ggtgatggtc tctcaaatcc tttttcataa attcccatca aaagttctct ttttaaaaag tagtcctcaa aatcactacc ctttgtttta gttacatcgt ctgtcttgaa cctaggatct ttagggggga tgttcaagcc cttcatatca ttacttaatc tttcagtctt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSALYT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGACTGAA AGATTAAGTA ATGATATGAA GGGCTTGAAC ATCCCTCCTA AAGATCCTAG GTTCAAGACA GACGATGTAA CTAAAACAAA GGGTAGTGAT TTTGAGGACT ACTTTTTAAA AAGAGAACTT TTGATGGGAA TTTATGAAAA AGGATTTGAG AGACCATCAC CAATTCAAGA AGAAAGTATA CCAGTGGCTC TTGCAGGCAA AGATATCCTA GCAAGAGCAA AGAATGGAAC AGGTAAGACT GCAGCATTTG TTATACCATT ATTAGAGAAA ATCAATACTA AAAAGAACAT TATTCAAGGC TTGATTCTTG TCCCAACAAG AGAACTTGCT TTACAAACTA GCAGTATTGT GAAGCAGCTT GGTAAGCATA TCAATGTCCA GTGTATGGTT AGTACTGGTG GTACATCTTT AAGAGATGAT ATATTACGTT TAAACAACCC AGTTCATGTA TTGGTAGGAA CTCCTGGTCG TATTTTGGAT CTGTCTAATA AGAAGGTGTG TAACTTGAGT GGTTGTTTTA TGTTTATTAT GGATGAGGCC GATAAATTAT TAAGTCCAGA ATTTCAGCCA ATTATTGAGG AACTCATAGA GTTTTTACCA AAAGAAAGGC AAATTCTCTT ATATTCTGCT ACTTTCCCCG TAACTGTTAA AGGTTTTAAG GATAAATATC TTGCAAATGC TCATGAAATT AATCTAATGG ACGAGCTCAC TTTAAAGGGT GTTACACAAT ATTATGCTTT TGTTGAAGAG AAGCAGAAAT TACATTGTCT TAATACTTTA TTTAGTAAAC TACAAATTAA CCAAGCCATT ATATTTTGTA ATTCTGTTAC AAGAGTTGAA CTTTTAGCAA AGAAAATTAC TGAGCTTGGA AGCTCATGCT TTTATATTCA CGCAAGAATG CTCCAGTCTC ACAGAAATAG AGTATTTCAT GACTTCAGAA ACGGTGCATG TAGGTGTCTT GTATCATCTG ATCTTATCAC AAGAGGTATT GATATCCAAT CTGTTAATGT CGTTATTAAT TTTGACTTCC CTAAATATTC CGAAACATAC CTTCACAGAA TTGGTAGATC TGGGAGATTT GGGCATCTTG GGCTCGCAAT CAACCTTATA ACTTATGAAG ATCGCTATAA CTTATATAGA ATTGAAAAAG AATTGGCCAC AGAGATTTCA CCAATTCCCG CCCAAATTGA TTCTGCCTTA TATACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for CrpaA.01586.a.A1.PW27275
PURIFICATION DATe: 6/23/2009
CONCENTRATION: 1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSAL
Validated NT Sequence
ataaggcaga atcaatttgg gcgggaattg gtgaaatctc tgtggccaat tctttttcaa ttctatataa gttatagcga tcttcataag ttataaggtt gattgcgagc ccaagatgcc caaatctccc agatctacca attctgtgaa ggtatgtttc ggaatattta gggaagtcaa aattaataac gacattaaca gattggatat caatacctct tgtgataaga tcagatgata caagacacct acatgcaccg tttctgaagt catgaaatac tctatttctg tgagactgga gcattcttgc gtgaatataa aagcatgagc ttccaagctc agtaattttc tttgctaaaa gttcaactct tgtaacagaa ttacaaaata taatggcttg gttaatttgt agtttactaa ataaagtatt aagacaatgt aatttctgct tctcttcaac aaaagcataa tattgtgtaa caccctttaa agtgagctcg tccattagat taatttcatg agcatttgca agatatttat ccttaaaacc tttaacagtt acggggaaag tagcagaata taagagaatt tgcctttctt ttggtaaaaa ctctatgagt tcctcaataa ttggctgaaa ttctggactt aataatttat cggcctcatc cataataaac ataaaacaac cactcaagtt acacaccttc ttattagaca gatccaaaat acgaccagga gttcctacca atacatgaac tgggttgttt aaacgtaata tatcatctct taaagatgta ccaccagtac taaccataca ctggacattg atatgcttac caagctgctt cacaatactg ctagtttgta aagcaagttc tcttgttggg acaagaatca agccttgaat aatgttcttt ttagtattga ttttctctaa taatggtata acaaatgctg cagtcttacc tgttccattc tttgctcttg ctaggatatc tttgcctgca agagccactg gtatactttc ttcttgaatt ggtgatggtc tctcaaatcc tttttcataa attcccatca aaagttctct ttttaaaaag tagtcctcaa aatcactacc ctttgtttta gttacatcgt ctgtcttgaa cctaggatct ttagggggga tgttcaagcc cttcatatca ttacttaatc tttcagtctt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTERLSND MKGLNIPPKD PRFKTDDVTK TKGSDFEDYF LKRELLMGIY EKGFERPSPI QEESIPVALA GKDILARAKN GTGKTAAFVI PLLEKINTKK NIIQGLILVP TRELALQTSS IVKQLGKHIN VQCMVSTGGT SLRDDILRLN NPVHVLVGTP GRILDLSNKK VCNLSGCFMF IMDEADKLLS PEFQPIIEEL IEFLPKERQI LLYSATFPVT VKGFKDKYLA NAHEINLMDE LTLKGVTQYY AFVEEKQKLH CLNTLFSKLQ INQAIIFCNS VTRVELLAKK ITELGSSCFY IHARMLQSHR NRVFHDFRNG ACRCLVSSDL ITRGIDIQSV NVVINFDFPK YSETYLHRIG RSGRFGHLGL AINLITYEDR YNLYRIEKEL ATEISPIPAQ IDSALYT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGACTGAA AGATTAAGTA ATGATATGAA GGGCTTGAAC ATCCCTCCTA AAGATCCTAG GTTCAAGACA GACGATGTAA CTAAAACAAA GGGTAGTGAT TTTGAGGACT ACTTTTTAAA AAGAGAACTT TTGATGGGAA TTTATGAAAA AGGATTTGAG AGACCATCAC CAATTCAAGA AGAAAGTATA CCAGTGGCTC TTGCAGGCAA AGATATCCTA GCAAGAGCAA AGAATGGAAC AGGTAAGACT GCAGCATTTG TTATACCATT ATTAGAGAAA ATCAATACTA AAAAGAACAT TATTCAAGGC TTGATTCTTG TCCCAACAAG AGAACTTGCT TTACAAACTA GCAGTATTGT GAAGCAGCTT GGTAAGCATA TCAATGTCCA GTGTATGGTT AGTACTGGTG GTACATCTTT AAGAGATGAT ATATTACGTT TAAACAACCC AGTTCATGTA TTGGTAGGAA CTCCTGGTCG TATTTTGGAT CTGTCTAATA AGAAGGTGTG TAACTTGAGT GGTTGTTTTA TGTTTATTAT GGATGAGGCC GATAAATTAT TAAGTCCAGA ATTTCAGCCA ATTATTGAGG AACTCATAGA GTTTTTACCA AAAGAAAGGC AAATTCTCTT ATATTCTGCT ACTTTCCCCG TAACTGTTAA AGGTTTTAAG GATAAATATC TTGCAAATGC TCATGAAATT AATCTAATGG ACGAGCTCAC TTTAAAGGGT GTTACACAAT ATTATGCTTT TGTTGAAGAG AAGCAGAAAT TACATTGTCT TAATACTTTA TTTAGTAAAC TACAAATTAA CCAAGCCATT ATATTTTGTA ATTCTGTTAC AAGAGTTGAA CTTTTAGCAA AGAAAATTAC TGAGCTTGGA AGCTCATGCT TTTATATTCA CGCAAGAATG CTCCAGTCTC ACAGAAATAG AGTATTTCAT GACTTCAGAA ACGGTGCATG TAGGTGTCTT GTATCATCTG ATCTTATCAC AAGAGGTATT GATATCCAAT CTGTTAATGT CGTTATTAAT TTTGACTTCC CTAAATATTC CGAAACATAC CTTCACAGAA TTGGTAGATC TGGGAGATTT GGGCATCTTG GGCTCGCAAT CAACCTTATA ACTTATGAAG ATCGCTATAA CTTATATAGA ATTGAAAAAG AATTGGCCAC AGAGATTTCA CCAATTCCCG CCCAAATTGA TTCTGCCTTA TATACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA