CrpaA.01523.a

Arginine n-methyltransferase

CENTER ID:	CrpaA.01523.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01523.a.A1.GE26556	Full length( CrpaA.01523.a )		1	348

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01523.a.A1.PW26601	Full length( CrpaA.01523.a )		1	348

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd8_4760
OrthoMCL:	OG5_127422
RefSeq:	XP_627360.1
UniProt:	Q5CVA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKPTEEELK EFKNSWNPLV QEDISSADYY FNSYAHFGIH EEMLKDSVRT GSYQKAIMSN KHLFQDKIVL DVGSGTGILC MFAAMAGAKH VYGIECSEIV HVARNIIKDN NLSDKITFIQ SKAEEAVLPV EKVDIIISEW MGYLLLYESM LDTVLFCRDK WLKEDGLIFP DRAQMYIAGI EDAEYKQEKL GYWNNVYGFN YQYVKPCIME EPIIDTVGEN AVNTSSYCIL DIDLYKCKKE DLEFVSPFYL KAARKDFVHA FIVWFDIIFG CGHKPVTFTT SPFGKYTHWK QSVFYFEEDL VCDTNDMISG IFALKKNTKN VRDLDIKIKF NFTKNNSSIE RINYYRLR

NT Sequence

ATGATTAAAC CAACAGAAGA AGAGCTTAAA GAGTTTAAGA ACTCCTGGAA TCCATTAGTT CAAGAGGATA TTAGCAGTGC AGATTATTAC TTTAATTCTT ATGCTCATTT TGGAATACAT GAAGAAATGT TAAAAGATTC AGTCAGAACT GGTTCATACC AGAAGGCCAT AATGTCTAAT AAACATTTGT TCCAAGATAA GATTGTCTTG GATGTAGGAA GTGGTACAGG AATTCTATGT ATGTTTGCTG CAATGGCAGG AGCAAAGCAC GTATATGGTA TAGAATGTTC AGAAATTGTA CATGTTGCCA GAAATATAAT TAAGGATAAT AATCTATCTG ATAAGATTAC CTTTATACAG AGTAAAGCTG AAGAAGCTGT TCTTCCTGTA GAAAAGGTTG ATATTATCAT TTCCGAATGG ATGGGATATT TACTATTATA TGAATCTATG TTGGATACTG TATTATTTTG CAGAGATAAA TGGCTTAAGG AAGATGGTTT AATCTTCCCA GATAGAGCAC AAATGTATAT TGCAGGTATC GAAGATGCTG AATATAAACA AGAAAAGCTT GGATATTGGA ATAATGTTTA TGGATTTAAT TATCAATATG TCAAACCTTG TATTATGGAA GAACCAATAA TAGATACCGT TGGAGAGAAT GCTGTTAATA CAAGCTCTTA TTGTATTCTT GATATTGACC TTTACAAGTG TAAGAAAGAA GACTTAGAAT TTGTATCTCC TTTTTATCTT AAGGCTGCTA GAAAAGACTT TGTTCATGCT TTCATTGTAT GGTTTGATAT CATTTTTGGA TGTGGTCATA AACCTGTTAC TTTTACTACA AGCCCTTTTG GGAAATATAC TCATTGGAAA CAATCAGTAT TCTACTTTGA AGAAGATCTT GTTTGCGATA CTAATGATAT GATTTCAGGA ATATTTGCTT TAAAGAAGAA CACTAAAAAT GTCCGAGATC TTGACATTAA AATCAAATTC AACTTTACAA AAAATAATTC TTCTATTGAA AGGATTAACT ATTATAGACT AAGG

Details for CrpaA.01523.a.A1.GE26556

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIKPTEEEL KEFKNSWNPL VQEDISSADY YFNSYAHFGI HEEMLKDSVR TGSYQKAIMS NKHLFQDKIV LDVGSGTGIL CMFAAMAGAK HVYGIECSEI VHVARNIIKD NNLSDKITFI QSKAEEAVLP VEKVDIIISE WMGYLLLYES MLDTVLFCRD KWLKEDGLXF PDRAQMYIAG IEDAEYKQEK LGYWNNVYGF NYQYVKPCIM EEPIIDTVGE NAVNTSSYCI LDIDLYKCKK EDLEFVSPFY LKAARKDFVH AFIVWFDIIF GCGHKPVTFT TSPFGKYTHW KQSVFYFEED LVCDTNDMIS GIFALKKNTK NVRDLDIKIK FNFTKNNSSI ERINYYRLR

Validated NT Sequence

ttattacctt agtctataat agttaatcct ttcaatagaa gaattatttt ttgtaaagtt gaatttgatt ttaatgtcaa gatctcggac atttttagtg ttcttcttta aagcaaatat tcctgaaatc atatcattag tatcgcaaac aagatcttct tcaaagtaga atactgattg tttccaatga gtatatttcc caaaagggct tgtagtaaaa gtaacaggtt tatgaccaca tccaaaaatg atatcaaacc atacaatgaa agcatgaaca aagtcttttc tagcagcctt aagataaaaa ggagatacaa attctaagtc ttctttctta cacttgtaaa ggtcaatatc aagaatacaa taagagcttg tattaacagc attctctcca acggtatcta ttattggttc ttccataata caaggtttga catattgata attaaatcca taaacattat tccaatatcc aagcttttct tgtttatatt cagcatcttc gatacctgca atatacattt gtgctctatc tgggaagant aaaccatctt ccttaagcca tttatctctg caaaataata cagtatccaa catagattca tataatagta aatatcccat ccattcggaa atgataatat caaccttttc tacaggaaga acagcttctt cagctttact ctgtataaag gtaatcttat cagatagatt attatcctta attatatttc tggcaacatg tacaatttct gaacattcta taccatatac gtgctttgct cctgccattg cagcaaacat acatagaatt cctgtaccac ttcctacatc caagacaatc ttatcttgga acaaatgttt attagacatt atggccttct ggtatgaacc agttctgact gaatctttta acatttcttc atgtattcca aaatgagcat aagaattaaa gtaataatct gcactgctaa tatcctcttg aactaatgga ttccaggagt tcttaaactc tttaagctct tcttctgttg gtttaatcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIKPTEEEL KEFKNSWNPL VQEDISSADY YFNSYAHFGI HEEMLKDSVR TGSYQKAIMS NKHLFQDKIV LDVGSGTGIL CMFAAMAGAK HVYGIECSEI VHVARNIIKD NNLSDKITFI QSKAEEAVLP VEKVDIIISE WMGYLLLYES MLDTVLFCRD KWLKEDGLIF PDRAQMYIAG IEDAEYKQEK LGYWNNVYGF NYQYVKPCIM EEPIIDTVGE NAVNTSSYCI LDIDLYKCKK EDLEFVSPFY LKAARKDFVH AFIVWFDIIF GCGHKPVTFT TSPFGKYTHW KQSVFYFEED LVCDTNDMIS GIFALKKNTK NVRDLDIKIK FNFTKNNSSI ERINYYRLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattaaacca acagaagaag agcttaaaga gtttaagaac tcctggaatc cattagttca agaggatatt agcagtgcag attattactt taattcttat gctcattttg gaatacatga agaaatgtta aaagattcag tcagaactgg ttcataccag aaggccataa tgtctaataa acatttgttc caagataaga ttgtcttgga tgtaggaagt ggtacaggaa ttctatgtat gtttgctgca atggcaggag caaagcacgt atatggtata gaatgttcag aaattgtaca tgttgccaga aatataatta aggataataa tctatctgat aagattacct ttatacagag taaagctgaa gaagctgttc ttcctgtaga aaaggttgat attatcattt ccgaatggat gggatattta ctattatatg aatctatgtt ggatactgta ttattttgca gagataaatg gcttaaggaa gatggtttaa tcttcccaga tagagcacaa atgtatattg caggtatcga agatgctgaa tataaacaag aaaagcttgg atattggaat aatgtttatg gatttaatta tcaatatgtc aaaccttgta ttatggaaga accaataata gataccgttg gagagaatgc tgttaataca agctcttatt gtattcttga tattgacctt tacaagtgta agaaagaaga cttagaattt gtatctcctt tttatcttaa ggctgctaga aaagactttg ttcatgcttt cattgtatgg tttgatatca tttttggatg tggtcataaa cctgttactt ttactacaag cccttttggg aaatatactc attggaaaca atcagtattc tactttgaag aagatcttgt ttgcgatact aatgatatga tttcaggaat atttgcttta aagaagaaca ctaaaaatgt ccgagatctt gacattaaaa tcaaattcaa ctttacaaaa aataattctt ctattgaaag gattaactat tatagactaa ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for CrpaA.01523.a.A1.PW26601

PURIFICATION DATe:	6/16/2009
CONCENTRATION:	25.49mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIKPTEEEL KEFKNSWNPL VQEDISSADY YFNSYAHFGI HEEMLKDSVR TGSYQKAIMS NKHLFQDKIV LDVGSGTGIL CMFAAMAGAK HVYGIECSEI VHVARNIIKD NNLSDKITFI QSKAEEAVLP VEKVDIIISE WMGYLLLYES MLDTVLFCRD KWLKEDGLIF PDRAQMYIAG IEDAEYKQEK LGYWNNVYGF NYQYVKPCIM EEPIIDTVGE NAVNTSSYCI LDIDLYKCKK EDLEFVSPFY LKAARKDFVH AFIVWFDIIF GCGHKPVTFT TSPFGKYTHW KQSVFYFEED LVCDTNDMIS GIFALKKNTK NVRDLDIKIK FNFTKNNSSI ERINYYRLR

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTAAACCA ACAGAAGAAG AGCTTAAAGA GTTTAAGAAC TCCTGGAATC CATTAGTTCA AGAGGATATT AGCAGTGCAG ATTATTACTT TAATTCTTAT GCTCATTTTG GAATACATGA AGAAATGTTA AAAGATTCAG TCAGAACTGG TTCATACCAG AAGGCCATAA TGTCTAATAA ACATTTGTTC CAAGATAAGA TTGTCTTGGA TGTAGGAAGT GGTACAGGAA TTCTATGTAT GTTTGCTGCA ATGGCAGGAG CAAAGCACGT ATATGGTATA GAATGTTCAG AAATTGTACA TGTTGCCAGA AATATAATTA AGGATAATAA TCTATCTGAT AAGATTACCT TTATACAGAG TAAAGCTGAA GAAGCTGTTC TTCCTGTAGA AAAGGTTGAT ATTATCATTT CCGAATGGAT GGGATATTTA CTATTATATG AATCTATGTT GGATACTGTA TTATTTTGCA GAGATAAATG GCTTAAGGAA GATGGTTTAA TCTTCCCAGA TAGAGCACAA ATGTATATTG CAGGTATCGA AGATGCTGAA TATAAACAAG AAAAGCTTGG ATATTGGAAT AATGTTTATG GATTTAATTA TCAATATGTC AAACCTTGTA TTATGGAAGA ACCAATAATA GATACCGTTG GAGAGAATGC TGTTAATACA AGCTCTTATT GTATTCTTGA TATTGACCTT TACAAGTGTA AGAAAGAAGA CTTAGAATTT GTATCTCCTT TTTATCTTAA GGCTGCTAGA AAAGACTTTG TTCATGCTTT CATTGTATGG TTTGATATCA TTTTTGGATG TGGTCATAAA CCTGTTACTT TTACTACAAG CCCTTTTGGG AAATATACTC ATTGGAAACA ATCAGTATTC TACTTTGAAG AAGATCTTGT TTGCGATACT AATGATATGA TTTCAGGAAT ATTTGCTTTA AAGAAGAACA CTAAAAATGT CCGAGATCTT GACATTAAAA TCAAATTCAA CTTTACAAAA AATAATTCTT CTATTGAAAG GATTAACTAT TATAGACTAA GGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA