CrpaA.01392.a

26S proteasome regulatory subunit 26b like AAA ATpase

CENTER ID:	CrpaA.01392.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01392.a.A1.GE26560	Full length( CrpaA.01392.a )		1	401

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd4_2540
OrthoMCL:	OG5_127831
RefSeq:	XP_625842.1
UniProt:	Q5CR10

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MACSLAKSQT IMSGNIDREK YLYGSLKELE KELNFLSIQE DYIKDEQKGL KREFQRAKEE IKRIQSVPLV IGQFLEMIDS RYGIVSSTAG SNYYVRVLST INREDLKPPA SVALHRHSHA IVDILPPEAD SSIQMLQMQE KPDVTYSDIG GMDIQKQEVR EAVELPLVCP ELYQQIGIDP PTGVLLYGPP GTGKTMLAKA VANHTTATFI RVVGSEFVQK YLGEGPRMVR DVFRLARENS PAIVFIDEVD SIATKRFDAQ TGADREVQRI LLELLNQMDG FDQTTNVKVI MATNRADTLD PALLRPGRLD RKIEFPLPDR RQRRLIFQTI TAKMNLSEEV DLEEYVSRPE KISAADIAAI SQEAGMQAVR KNRYVILPKD FEKGWKIHVK KSDRDFDFYS V

NT Sequence

ATGGCATGTT CTCTGGCCAA GTCGCAAACA ATTATGTCTG GCAATATAGA CCGTGAAAAA TATCTCTATG GAAGCCTTAA GGAGCTTGAG AAAGAACTCA ACTTTTTGAG TATTCAAGAA GATTATATCA AAGATGAACA AAAAGGTTTG AAACGTGAAT TCCAGCGTGC GAAGGAGGAA ATCAAACGTA TTCAATCAGT TCCGCTCGTT ATTGGGCAAT TTCTTGAAAT GATAGATTCC AGGTACGGAA TTGTTTCTTC TACAGCAGGC TCAAATTATT ATGTTAGGGT TCTTAGTACA ATCAATAGAG AAGATCTTAA ACCACCTGCG TCAGTCGCGC TTCATAGGCA CAGTCATGCA ATAGTTGATA TTCTTCCTCC TGAAGCAGAT TCTAGTATTC AAATGCTTCA AATGCAGGAA AAACCTGATG TAACATATTC TGATATTGGA GGGATGGATA TTCAAAAGCA AGAAGTACGA GAAGCCGTTG AGTTGCCACT TGTGTGTCCA GAATTATATC AACAAATAGG AATTGATCCT CCAACTGGAG TGCTTCTATA TGGACCTCCC GGAACTGGTA AAACTATGCT TGCTAAAGCT GTTGCTAATC ACACAACAGC CACGTTTATT AGAGTCGTTG GGTCAGAGTT TGTACAAAAA TATTTGGGTG AAGGCCCAAG GATGGTCCGC GACGTATTTC GTTTGGCAAG AGAAAACTCC CCAGCAATAG TGTTTATTGA TGAAGTAGAT TCAATTGCAA CTAAACGTTT TGATGCACAA ACTGGGGCAG ATAGGGAAGT TCAACGTATT TTGCTCGAGC TTTTAAACCA AATGGATGGA TTTGACCAAA CAACAAATGT AAAAGTAATT ATGGCGACTA ACAGAGCAGA TACCCTTGAT CCTGCGCTAT TGAGGCCCGG TAGACTTGAC CGAAAAATAG AATTCCCATT ACCTGATAGA AGGCAAAGGA GATTGATATT CCAGACTATT ACTGCAAAGA TGAACTTGAG TGAAGAAGTT GATCTTGAAG AATATGTCTC CAGACCAGAA AAAATATCTG CAGCTGATAT AGCCGCGATC TCTCAAGAGG CCGGTATGCA AGCTGTAAGG AAAAATAGAT ATGTTATTCT ACCTAAGGAT TTTGAAAAGG GATGGAAGAT ACACGTAAAA AAGAGTGATA GAGATTTTGA TTTCTATAGT GTA

Details for CrpaA.01392.a.A1.GE26560

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	47kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

ccctggaagc tcagacccag ggtcctggtt cgatggcatg ttctctggcc aagtcgcaaa caattatgtc tggcaatata gaccgtgaaa aatatctcta tggaagcctt aaggagcttg agaaagaact caactttttg agtattcaag aagattatat caaagatgaa caaaaaggtt tgaaacgtga attccagcgt gcgaaggagg aaatcaaacg tattcaatca gttccgctcg ttattgggca atttcttgaa atgatagatt ccaggtacgg aattgtttct tctacagcag gctcaaatta ttatgttagg gttcttagta caatcaatag agaagatctt aaaccacctg cgtcagtcgc gcttcatagg cacagtcatg caatagttga tattcttcct cctgaagcag attctagtat tcaaatgctt caaatgcagg aaaaacctga tgtaacatat tctgatattg gagggatgga tattcaaaag caagaagtac gagaagccgt tgagttgcca cttgtgtgtc cagaattata tcaacaaata ggaattgatc ctccaactgg agtgcttcta tatggacctc ccggaactgg taaaactatg cttgctaaag ctgttgctaa tcacacaaca gccacgttta ttagagtcgt tgggtcagag tttgtacaaa aatatttggg tgaaggccca aggatggtcc gcgacgtatt tcgtttggca agagaaaact ccccagcaat agtgtttatt gatgaagtag attcaattgc aactaaacgt tttgatgcac aaactggggc agatagggaa gttcaacgta ttttgctcga gcttttaaac caaatggatg gatttgacca aacaacaaat gtaaaagtaa ttatggcgac taacagagca gatacccttg atcctgcgct attgaggccc ggtagacttg accgaaaaat agaattccca ttacctgata gaaggcaaag gagattgata ttccagacta ttactgcaaa gatgaacttg agtgaagaag ttgatcttga agaatatgtc tccagaccag aaaaaatatc tgcagctgat atagccgcga tctctcaaga ggccggtatg caagctgtaa ggaaaaatag atatgttatt ctacctaagg attttgaaaa gggatggaag atacacgtaa aaaagagtga tagagatttt gatttctata gtgtataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMACSLAKSQ TIMSGNIDRE KYLYGSLKEL EKELNFLSIQ EDYIKDEQKG LKREFQRAKE EIKRIQSVPL VIGQFLEMID SRYGIVSSTA GSNYYVRVLS TINREDLKPP ASVALHRHSH AIVDILPPEA DSSIQMLQMQ EKPDVTYSDI GGMDIQKQEV REAVELPLVC PELYQQIGID PPTGVLLYGP PGTGKTMLAK AVANHTTATF IRVVGSEFVQ KYLGEGPRMV RDVFRLAREN SPAIVFIDEV DSIATKRFDA QTGADREVQR ILLELLNQMD GFDQTTNVKV IMATNRADTL DPALLRPGRL DRKIEFPLPD RRQRRLIFQT ITAKMNLSEE VDLEEYVSRP EKISAADIAA ISQEAGMQAV RKNRYVILPK DFEKGWKIHV KKSDRDFDFY SV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatgttct ctggccaagt cgcaaacaat tatgtctggc aatatagacc gtgaaaaata tctctatgga agccttaagg agcttgagaa agaactcaac tttttgagta ttcaagaaga ttatatcaaa gatgaacaaa aaggtttgaa acgtgaattc cagcgtgcga aggaggaaat caaacgtatt caatcagttc cgctcgttat tgggcaattt cttgaaatga tagattccag gtacggaatt gtttcttcta cagcaggctc aaattattat gttagggttc ttagtacaat caatagagaa gatcttaaac cacctgcgtc agtcgcgctt cataggcaca gtcatgcaat agttgatatt cttcctcctg aagcagattc tagtattcaa atgcttcaaa tgcaggaaaa acctgatgta acatattctg atattggagg gatggatatt caaaagcaag aagtacgaga agccgttgag ttgccacttg tgtgtccaga attatatcaa caaataggaa ttgatcctcc aactggagtg cttctatatg gacctcccgg aactggtaaa actatgcttg ctaaagctgt tgctaatcac acaacagcca cgtttattag agtcgttggg tcagagtttg tacaaaaata tttgggtgaa ggcccaagga tggtccgcga cgtatttcgt ttggcaagag aaaactcccc agcaatagtg tttattgatg aagtagattc aattgcaact aaacgttttg atgcacaaac tggggcagat agggaagttc aacgtatttt gctcgagctt ttaaaccaaa tggatggatt tgaccaaaca acaaatgtaa aagtaattat ggcgactaac agagcagata cccttgatcc tgcgctattg aggcccggta gacttgaccg aaaaatagaa ttcccattac ctgatagaag gcaaaggaga ttgatattcc agactattac tgcaaagatg aacttgagtg aagaagttga tcttgaagaa tatgtctcca gaccagaaaa aatatctgca gctgatatag ccgcgatctc tcaagaggcc ggtatgcaag ctgtaaggaa aaatagatat gttattctac ctaaggattt tgaaaaggga tggaagatac acgtaaaaaa gagtgataga gattttgatt tctatagtgt aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa