CrpaA.01391.a

Ser/Thr protein kinase, putative

CENTER ID: CrpaA.01391.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrpaA.01391.a.A1.GE41055 full length 1 669
CrpaA.01391.a.B2.GE41003 kinase domain truncation 1 196 490
CrpaA.01391.a.B3.GE41004 kinase domain truncation 2 169 521
CrpaA.01391.a.HW1.GE41059 full length 1 669
CrpaA.01391.a.HX1.GE41057 full length 1 669
CrpaA.01391.a.HZ1.GE41058 full length 1 669
CrpaA.01391.a.MB1.GE41056 full length 1 669
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd6_5060
RefSeq: XP_627829.1
UniProt: Q5CWH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYTIHEGCNI SGNTRIYDTP NRHTWDINNG GTDGMRNESS TPRFKHNFNS GYSDKKFLQH GEEMEFTGNN RNDILGRNGN LIRQNISEYK KSVGVMEGSY VDRNMEISET PRVIQQLETN KRQISEEFTE KRDHEYDTRI VDKNTKLSSK SGSQSSLNQT NYKKVNLGGF TEADWYKKIP NWERIIDERY TSVYSSYERL EMIGTGTYAK VWLLKDKITG KKYAGKLLEP HTYPSDTVER IERMFQSEIK NLIVSQCPGV VRLHKIVVGP EGSLLVQDYV DDGTIWRENC CVSELEAFQH FIQLIQSVLY FQDKGVVHRD LKPTNILRYS NKTIVIGDFG WSEQTKKLHL SPSEWPGTLE INPPEVLTFK GPLTEKIDNY AVGMNLLLFL TGRFICRQKG LDVTEAAPYI LKVVELIRSD FNNKILSKGK QKGNTQLKQS QYFMWEIFIG FTDPNPNTRW SIKKALFHPD CIQQLLFCFH NNFPILWHPQ VIAIIKSNII PNNVSSHQTF IEDEKQYCEV GKQSIGMGKS SMANINSSNQ INIHNVSHAT HFNNYSGGQS PVSEDLAESQ RSYYKSVGTS LRPANPPIGH KFFKNDGQQH KLMLNSQFLS GSSDNNSVYN NEFAASLRNQ QQVNTSSAAT LSYTNNGHKF VGVESSVVKQ PHFINNFHY
NT Sequence
atgtatacta ttcacgaagg gtgtaatata tcagggaaca caaggattta tgacactccg aacagacaca cttgggatat caataatgga ggtactgatg gtatgaggaa tgaaagttca acacctagat tcaagcataa ctttaattct ggttattctg ataaaaagtt tctacaacat ggagaagaga tggaatttac agggaacaat agaaatgata tattaggaag aaatggaaac ttaatcagac aaaatatatc agaatataaa aaaagcgttg gagtaatgga agggagctat gttgacagaa acatggagat ttcagaaacc ccaagagtaa ttcaacagtt agaaactaat aaacgtcaaa taagtgaaga atttactgag aaaagagatc atgaatatga taccagaatt gtagataaga acactaaatt gagtagcaaa agtggatctc aaagctcatt gaaccaaaca aactacaaga aggtgaactt gggaggattt actgaagcag attggtataa aaagattcca aattgggaaa gaataattga tgaaagatat acaagcgttt atagttcata tgaaagactt gaaatgatag gaactgggac ttatgctaaa gtttggcttt tgaaagataa aataacgggg aagaaatatg caggaaaact attagaacct catacttatc cttcagatac agtagaaagg attgagagaa tgtttcagtc agaaataaag aatctcatag taagccaatg tccaggtgta gttaggctgc acaagatcgt agtaggccca gaaggaagcc tattagttca agattatgta gatgatggta ctatttggcg tgagaactgt tgtgtaagcg aattggaagc cttccagcac tttattcaac taattcagag cgtattatac ttccaagaca agggggtagt acatagagac ttgaaaccaa cgaatatttt aagatattca aataaaacaa tagtaatagg tgattttggg tggagtgaac aaacgaagaa gttacatttg agcccctcag aatggccagg aacacttgaa attaatcccc cggaagtatt gacatttaaa ggtcctttaa ccgaaaagat agacaactat gcagttggaa tgaacttatt gttgtttctc actggtaggt ttatttgcag acagaaagga ttggatgtta cagaagcagc tccttatatt ctaaaagtag tggaactaat cagaagtgat tttaataata agattctgag taaaggaaaa caaaaaggga atactcaatt aaagcagagc cagtacttca tgtgggagat ctttattggg tttactgacc ccaacccaaa tacaaggtgg agtattaaga aggctttgtt tcacccagat tgtattcaac agcttttatt ttgtttccat aataactttc caattctatg gcatcctcag gtaattgcta taatcaaatc aaatattata cctaacaatg tgagctctca ccaaactttc atagaagatg agaaacaata ttgtgaagtt ggaaaacaaa gtattggtat gggaaagagt tcaatggcaa acataaatag tagtaatcag ataaatattc acaatgtttc acatgctaca cattttaata attatagtgg cggtcagagt ccagtttctg aagacttagc agaatcccag agaagctatt ataagtcagt tgggacgtct ctaaggcctg caaatcctcc aataggtcac aagttcttta aaaacgacgg acaacaacat aaactaatgc taaacagtca gtttttgtca gggtcttctg ataacaattc agtttataat aacgagtttg ctgcatcatt aagaaaccaa caacaggtga atacctcctc tgcagccact ctaagttata caaacaatgg ccataagttt gttggggttg aaagttctgt tgtgaagcaa ccccatttta tcaataattt tcactattga
Details for CrpaA.01391.a.A1.GE41055
HARVESTED ON: 1/9/2017
SEQUENCED ON: 1/6/2017
EXPECTED MW: 79kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYTIHEGCN ISGNTRIYDT PNRHTWDINN GGTDGMRNES STPRFKHNFN SGYSDKKFLQ HGEEMEFTGN NRNDILGRNG NLIRQNISEY KKSVGVMEGS YVDRNMEISE TPRVIQQLET NKRQISEEFT EKRDHEYDTR IVDKNTKLSS KSGSQSSLNQ TNYKKVNLGG FTEADWYKKI PNWERIIDER YTSVYSSYER LEMIGTGTYA KVWLLKDKIT GKKYAGKLLE PHTYPSDTVE RIERMFQSEI KNLIVSQCPG VVRLHKIVVG PEGSLLVQDY VDDGTIWREN CCVSELEAFQ HFIQLIQSVL YFQDKGVVHR DLKPTNILRY SNKTIVIGDF GWSEQTKKLH LSPSEWPGTL EINPPEVLTF KGPLTEKIDN YAVGMNLLLF LTGRFICRQK GLDVTEAAPY ILKVVELIRS DFNNKILSKG KQKGNTQLKQ SQYFMWEIFI GFTDPNPNTR WSIKKALFHP DCIQQLLFCF HNNFPILWHP QVIAIIKSNI IPNNVSSHQT FIEDEKQYCE VGKQSIGMGK SSMANINSSN QINIHNVSHA THFNNYSGGQ SPVSEDLAES QRSYYKSVGT SLRPANPPIG HKFFKNDGQQ HKLMLNSQFL SGSSDNNSVY NNEFAASLRN QQQVNTSSAA TLSYTNNGHK FVGVESSVVK QPHFINNFHY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtatactatt cacgaagggt gtaatatatc agggaacaca aggatttatg acactccgaa cagacacact tgggatatca ataatggagg tactgatggt atgaggaatg aaagttcaac acctagattc aagcataact ttaattctgg ttattctgat aaaaagtttc tacaacatgg agaagagatg gaatttacag ggaacaatag aaatgatata ttaggaagaa atggaaactt aatcagacaa aatatatcag aatataaaaa aagcgttgga gtaatggaag ggagctatgt tgacagaaac atggagattt cagaaacccc aagagtaatt caacagttag aaactaataa acgtcaaata agtgaagaat ttactgagaa aagagatcat gaatatgata ccagaattgt agataagaac actaaattga gtagcaaaag tggatctcaa agctcattga accaaacaaa ctacaagaag gtgaacttgg gaggatttac tgaagcagat tggtataaaa agattccaaa ttgggaaaga ataattgatg aaagatatac aagcgtttat agttcatatg aaagacttga aatgatagga actgggactt atgctaaagt ttggcttttg aaagataaaa taacggggaa gaaatatgca ggaaaactat tagaacctca tacttatcct tcagatacag tagaaaggat tgagagaatg tttcagtcag aaataaagaa tctcatagta agccaatgtc caggtgtagt taggctgcac aagatcgtag taggcccaga aggaagccta ttagttcaag attatgtaga tgatggtact atttggcgtg agaactgttg tgtaagcgaa ttggaagcct tccagcactt tattcaacta attcagagcg tattatactt ccaagacaag ggggtagtac atagagactt gaaaccaacg aatattttaa gatattcaaa taaaacaata gtaataggtg attttgggtg gagtgaacaa acgaagaagt tacatttgag cccctcagaa tggccaggaa cacttgaaat taatcccccg gaagtattga catttaaagg tcctttaacc gaaaagatag acaactatgc agttggaatg aacttattgt tgtttctcac tggtaggttt atttgcagac agaaaggatt ggatgttaca gaagcagctc cttatattct aaaagtagtg gaactaatca gaagtgattt taataataag attctgagta aaggaaaaca aaaagggaat actcaattaa agcagagcca gtacttcatg tgggagatct ttattgggtt tactgacccc aacccaaata caaggtggag tattaagaag gctttgtttc acccagattg tattcaacag cttttatttt gtttccataa taactttcca attctatggc atcctcaggt aattgctata atcaaatcaa atattatacc taacaatgtg agctctcacc aaactttcat agaagatgag aaacaatatt gtgaagttgg aaaacaaagt attggtatgg gaaagagttc aatggcaaac ataaatagta gtaatcagat aaatattcac aatgtttcac atgctacaca ttttaataat tatagtggcg gtcagagtcc agtttctgaa gacttagcag aatcccagag aagctattat aagtcagttg ggacgtctct aaggcctgca aatcctccaa taggtcacaa gttctttaaa aacgacggac aacaacataa actaatgcta aacagtcagt ttttgtcagg gtcttctgat aacaattcag tttataataa cgagtttgct gcatcattaa gaaaccaaca acaggtgaat acctcctctg cagccactct aagttataca aacaatggcc ataagtttgt tggggttgaa agttctgttg tgaagcaacc ccattttatc aataattttc actattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for CrpaA.01391.a.B2.GE41003
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 35kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS YERLEMIGTG TYAKVWLLKD KITGKKYAGK LLEPHTYPSD TVERIERMFQ SEIKNLIVSQ CPGVVRLHKI VVGPEGSLLV QDYVDDGTIW RENCCVSELE AFQHFIQLIQ SVLYFQDKGV VHRDLKPTNI LRYSNKTIVI GDFGWSEQTK KLHLSPSEWP GTLEINPPEV LTFKGPLTEK IDNYAVGMNL LLFLTGRFIC RQKGLDVTEA APYILKVVEL IRSDFNNKIL SKGKQKGNTQ LKQSQYFMWE IFIGFTDPNP NTRWSIKKAL FHPDCIQQLL FCFHNN
Validated NT Sequence
agttattatg gaaacaaaat aaaagctgtt gaatacaatc tgggtgaaac aaagccttct taatactcca ccttgtattt gggttggggt cagtaaaccc aataaagatc tcccacatga agtactggct ctgctttaat tgagtattcc ctttttgttt tcctttactc agaatcttat tattaaaatc acttctgatt agttccacta cttttagaat ataaggagct gcttctgtaa catccaatcc tttctgtctg caaataaacc taccagtgag aaacaacaat aagttcattc caactgcata gttgtctatc ttttcggtta aaggaccttt aaatgtcaat acttccgggg gattaatttc aagtgttcct ggccattctg aggggctcaa atgtaacttc ttcgtttgtt cactccaccc aaaatcacct attactattg ttttatttga atatcttaaa atattcgttg gtttcaagtc tctatgtact acccccttgt cttggaagta taatacgctc tgaattagtt gaataaagtg ctggaaggct tccaattcgc ttacacaaca gttctcacgc caaatagtac catcatctac ataatcttga actaataggc ttccttctgg gcctactacg atcttgtgca gcctaactac acctggacat tggcttacta tgagattctt tatttctgac tgaaacattc tctcaatcct ttctactgta tctgaaggat aagtatgagg ttctaatagt tttcctgcat atttcttccc cgttatttta tctttcaaaa gccaaacttt agcataagtc ccagttccta tcatttcaag tctttcatat gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHSY ERLEMIGTGT YAKVWLLKDK ITGKKYAGKL LEPHTYPSDT VERIERMFQS EIKNLIVSQC PGVVRLHKIV VGPEGSLLVQ DYVDDGTIWR ENCCVSELEA FQHFIQLIQS VLYFQDKGVV HRDLKPTNIL RYSNKTIVIG DFGWSEQTKK LHLSPSEWPG TLEINPPEVL TFKGPLTEKI DNYAVGMNLL LFLTGRFICR QKGLDVTEAA PYILKVVELI RSDFNNKILS KGKQKGNTQL KQSQYFMWEI FIGFTDPNPN TRWSIKKALF HPDCIQQLLF CFHNNFPILW HPQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcatatg aaagacttga aatgatagga actgggactt atgctaaagt ttggcttttg aaagataaaa taacggggaa gaaatatgca ggaaaactat tagaacctca tacttatcct tcagatacag tagaaaggat tgagagaatg tttcagtcag aaataaagaa tctcatagta agccaatgtc caggtgtagt taggctgcac aagatcgtag taggcccaga aggaagccta ttagttcaag attatgtaga tgatggtact atttggcgtg agaactgttg tgtaagcgaa ttggaagcct tccagcactt tattcaacta attcagagcg tattatactt ccaagacaag ggggtagtac atagagactt gaaaccaacg aatattttaa gatattcaaa taaaacaata gtaataggtg attttgggtg gagtgaacaa acgaagaagt tacatttgag cccctcagaa tggccaggaa cacttgaaat taatcccccg gaagtattga catttaaagg tcctttaacc gaaaagatag acaactatgc agttggaatg aacttattgt tgtttctcac tggtaggttt atttgcagac agaaaggatt ggatgttaca gaagcagctc cttatattct aaaagtagtg gaactaatca gaagtgattt taataataag attctgagta aaggaaaaca aaaagggaat actcaattaa agcagagcca gtacttcatg tgggagatct ttattgggtt tactgacccc aacccaaata caaggtggag tattaagaag gctttgtttc acccagattg tattcaacag cttttatttt gtttccataa taactttcca attctatggc atcctcagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrpaA.01391.a.B3.GE41004
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG FTEADWYKKI PNWERIIDER YTSVYSSYER LEMIGTGTYA KVWLLKDKIT GKKYAGKLLE PHTYPSDTVE RIERMFQSEI KNLIVSQCPG VVRLHKIVVG PEGSLLVQDY VDDGTIWREN CCVSELEAFQ HFIQLIQSVL YFQDKGVVHR DLKPTNILRY SNKTIVIGDF GWSEQTKKLH LSPSEWPGTL EINPPEVLTF KGPLTEKIDN YAVGMNLLLF LTGRFICRQK GLDVTEAAPY ILKVVELIRS DFNNKILSKG KQKGNTQLKQ SQYFMWEIFI GFTDPNPNTR WSIKKALFHP DCIQQLLFCF HNNFPILWHP QVIAIIKSNI IPNNVSSHQT FIED
Validated NT Sequence
catcttctat gaaagtttgg tgagagctca cattgttagg tataatattt gatttgatta tagcaattac ctgaggatgc catagaattg gaaagttatt atggaaacaa aataaaagct gttgaataca atctgggtga aacaaagcct tcttaatact ccaccttgta tttgggttgg ggtcagtaaa cccaataaag atctcccaca tgaagtactg gctctgcttt aattgagtat tccctttttg ttttccttta ctcagaatct tattattaaa atcacttctg attagttcca ctacttttag aatataagga gctgcttctg taacatccaa tcctttctgt ctgcaaataa acctaccagt gagaaacaac aataagttca ttccaactgc atagttgtct atcttttcgg ttaaaggacc tttaaatgtc aatacttccg ggggattaat ttcaagtgtt cctggccatt ctgaggggct caaatgtaac ttcttcgttt gttcactcca cccaaaatca cctattacta ttgttttatt tgaatatctt aaaatattcg ttggtttcaa gtctctatgt actaccccct tgtcttggaa gtataatacg ctctgaatta gttgaataaa gtgctggaag gcttccaatt cgcttacaca acagttctca cgccaaatag taccatcatc tacataatct tgaactaata ggcttccttc tgggcctact acgatcttgt gcagcctaac tacacctgga cattggctta ctatgagatt ctttatttct gactgaaaca ttctctcaat cctttctact gtatctgaag gataagtatg aggttctaat agttttcctg catatttctt ccccgttatt ttatctttca aaagccaaac tttagcataa gtcccagttc ctatcatttc aagtctttca tatgaactat aaacgcttgt atatctttca tcaattattc tttcccaatt tggaatcttt ttataccaat ctgcttcagt aaatcccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHGF TEADWYKKIP NWERIIDERY TSVYSSYERL EMIGTGTYAK VWLLKDKITG KKYAGKLLEP HTYPSDTVER IERMFQSEIK NLIVSQCPGV VRLHKIVVGP EGSLLVQDYV DDGTIWRENC CVSELEAFQH FIQLIQSVLY FQDKGVVHRD LKPTNILRYS NKTIVIGDFG WSEQTKKLHL SPSEWPGTLE INPPEVLTFK GPLTEKIDNY AVGMNLLLFL TGRFICRQKG LDVTEAAPYI LKVVELIRSD FNNKILSKGK QKGNTQLKQS QYFMWEIFIG FTDPNPNTRW SIKKALFHPD CIQQLLFCFH NNFPILWHPQ VIAIIKSNII PNNVSSHQTF IEDEKQYCEV G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggattta ctgaagcaga ttggtataaa aagattccaa attgggaaag aataattgat gaaagatata caagcgttta tagttcatat gaaagacttg aaatgatagg aactgggact tatgctaaag tttggctttt gaaagataaa ataacgggga agaaatatgc aggaaaacta ttagaacctc atacttatcc ttcagataca gtagaaagga ttgagagaat gtttcagtca gaaataaaga atctcatagt aagccaatgt ccaggtgtag ttaggctgca caagatcgta gtaggcccag aaggaagcct attagttcaa gattatgtag atgatggtac tatttggcgt gagaactgtt gtgtaagcga attggaagcc ttccagcact ttattcaact aattcagagc gtattatact tccaagacaa gggggtagta catagagact tgaaaccaac gaatatttta agatattcaa ataaaacaat agtaataggt gattttgggt ggagtgaaca aacgaagaag ttacatttga gcccctcaga atggccagga acacttgaaa ttaatccccc ggaagtattg acatttaaag gtcctttaac cgaaaagata gacaactatg cagttggaat gaacttattg ttgtttctca ctggtaggtt tatttgcaga cagaaaggat tggatgttac agaagcagct ccttatattc taaaagtagt ggaactaatc agaagtgatt ttaataataa gattctgagt aaaggaaaac aaaaagggaa tactcaatta aagcagagcc agtacttcat gtgggagatc tttattgggt ttactgaccc caacccaaat acaaggtgga gtattaagaa ggctttgttt cacccagatt gtattcaaca gcttttattt tgtttccata ataactttcc aattctatgg catcctcagg taattgctat aatcaaatca aatattatac ctaacaatgt gagctctcac caaactttca tagaagatga gaaacaatat tgtgaagttg gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrpaA.01391.a.HW1.GE41059
HARVESTED ON: 1/9/2017
SEQUENCED ON: 1/6/2017
EXPECTED MW: 91kDa
OBSERVED MW: 91kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGALSKGQLK EFLDANLAGT LEAQTQGPGS MYTIHEGCNI SGNTRIYDTP NRHTWDINNG GTDGMRNESS TPRFKHNFNS GYSDKKFLQH GEEMEFTGNN RNDILGRNGN LIRQNISEYK KSVGVMEGSY VDRNMEISET PRVIQQLETN KRQISEEFTE KRDHEYDTRI VDKNTKLSSK SGSQSSLNQT NYKKVNLGGF TEADWYKKIP NWERIIDERY TSVYSSYERL EMIGTGTYAK VWLLKDKITG KKYAGKLLEP HTYPSDTVER IERMFQSEIK NLIVSQCPGV VRLHKIVVGP EGSLLVQDYV DDGTIWRENC CVSELEAFQH FIQLIQSVLY FQDKGVVHRD LKPTNILRYS NKTIVIGDFG WSEQTKKLHL SPSEWPGTLE INPPEVLTFK GPLTEKIDNY AVGMNLLLFL TGRFICRQKG LDVTEAAPYI LKVVELIRSD FNNKILSKGK QKGNTQLKQS QYFMWEIFIG FTDPNPNTRW SIKKALFHPD CIQQLLFCFH NNFPILWHPQ VIAIIKSNII PNNVSSHQTF IEDEKQYCEV GKQSIGMGKS SMANINSSNQ INIHNVSHAT HFNNYSGGQS PVSEDLAESQ RSYYKSVGTS LRPANPPIGH KFFKNDGQQH KLMLNSQFLS GSSDNNSVYN NEFAASLRNQ QQVNTSSAAT LSYTNNGHKF VGVESSVVKQ PHFINNFHY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagagcga taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg gtaccctgga agctcagacc cagggtcctg gttcgatgta tactattcac gaagggtgta atatatcagg gaacacaagg atttatgaca ctccgaacag acacacttgg gatatcaata atggaggtac tgatggtatg aggaatgaaa gttcaacacc tagattcaag cataacttta attctggtta ttctgataaa aagtttctac aacatggaga agagatggaa tttacaggga acaatagaaa tgatatatta ggaagaaatg gaaacttaat cagacaaaat atatcagaat ataaaaaaag cgttggagta atggaaggga gctatgttga cagaaacatg gagatttcag aaaccccaag agtaattcaa cagttagaaa ctaataaacg tcaaataagt gaagaattta ctgagaaaag agatcatgaa tatgatacca gaattgtaga taagaacact aaattgagta gcaaaagtgg atctcaaagc tcattgaacc aaacaaacta caagaaggtg aacttgggag gatttactga agcagattgg tataaaaaga ttccaaattg ggaaagaata attgatgaaa gatatacaag cgtttatagt tcatatgaaa gacttgaaat gataggaact gggacttatg ctaaagtttg gcttttgaaa gataaaataa cggggaagaa atatgcagga aaactattag aacctcatac ttatccttca gatacagtag aaaggattga gagaatgttt cagtcagaaa taaagaatct catagtaagc caatgtccag gtgtagttag gctgcacaag atcgtagtag gcccagaagg aagcctatta gttcaagatt atgtagatga tggtactatt tggcgtgaga actgttgtgt aagcgaattg gaagccttcc agcactttat tcaactaatt cagagcgtat tatacttcca agacaagggg gtagtacata gagacttgaa accaacgaat attttaagat attcaaataa aacaatagta ataggtgatt ttgggtggag tgaacaaacg aagaagttac atttgagccc ctcagaatgg ccaggaacac ttgaaattaa tcccccggaa gtattgacat ttaaaggtcc tttaaccgaa aagatagaca actatgcagt tggaatgaac ttattgttgt ttctcactgg taggtttatt tgcagacaga aaggattgga tgttacagaa gcagctcctt atattctaaa agtagtggaa ctaatcagaa gtgattttaa taataagatt ctgagtaaag gaaaacaaaa agggaatact caattaaagc agagccagta cttcatgtgg gagatcttta ttgggtttac tgaccccaac ccaaatacaa ggtggagtat taagaaggct ttgtttcacc cagattgtat tcaacagctt ttattttgtt tccataataa ctttccaatt ctatggcatc ctcaggtaat tgctataatc aaatcaaata ttatacctaa caatgtgagc tctcaccaaa ctttcataga agatgagaaa caatattgtg aagttggaaa acaaagtatt ggtatgggaa agagttcaat ggcaaacata aatagtagta atcagataaa tattcacaat gtttcacatg ctacacattt taataattat agtggcggtc agagtccagt ttctgaagac ttagcagaat cccagagaag ctattataag tcagttggga cgtctctaag gcctgcaaat cctccaatag gtcacaagtt ctttaaaaac gacggacaac aacataaact aatgctaaac agtcagtttt tgtcagggtc ttctgataac aattcagttt ataataacga gtttgctgca tcattaagaa accaacaaca ggtgaatacc tcctctgcag ccactctaag ttatacaaac aatggccata agtttgttgg ggttgaaagt tctgttgtga agcaacccca ttttatcaat aattttcact ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for CrpaA.01391.a.HX1.GE41057
HARVESTED ON: 1/9/2017
SEQUENCED ON: 1/6/2017
EXPECTED MW: 134kDa
OBSERVED MW: 134kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMYTI HEGCNISGNT RIYDTPNRHT WDINNGGTDG MRNESSTPRF KHNFNSGYSD KKFLQHGEEM EFTGNNRNDI LGRNGNLIRQ NISEYKKSVG VMEGSYVDRN MEISETPRVI QQLETNKRQI SEEFTEKRDH EYDTRIVDKN TKLSSKSGSQ SSLNQTNYKK VNLGGFTEAD WYKKIPNWER IIDERYTSVY SSYERLEMIG TGTYAKVWLL KDKITGKKYA GKLLEPHTYP SDTVERIERM FQSEIKNLIV SQCPGVVRLH KIVVGPEGSL LVQDYVDDGT IWRENCCVSE LEAFQHFIQL IQSVLYFQDK GVVHRDLKPT NILRYSNKTI VIGDFGWSEQ TKKLHLSPSE WPGTLEINPP EVLTFKGPLT EKIDNYAVGM NLLLFLTGRF ICRQKGLDVT EAAPYILKVV ELIRSDFNNK ILSKGKQKGN TQLKQSQYFM WEIFIGFTDP NPNTRWSIKK ALFHPDCIQQ LLFCFHNNFP ILWHPQVIAI IKSNIIPNNV SSHQTFIEDE KQYCEVGKQS IGMGKSSMAN INSSNQINIH NVSHATHFNN YSGGQSPVSE DLAESQRSYY KSVGTSLRPA NPPIGHKFFK NDGQQHKLML NSQFLSGSSD NNSVYNNEFA ASLRNQQQVN TSSAATLSYT NNGHKFVGVE SSVVKQPHFI NNFHY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcgatgtata ctattcacga agggtgtaat atatcaggga acacaaggat ttatgacact ccgaacagac acacttggga tatcaataat ggaggtactg atggtatgag gaatgaaagt tcaacaccta gattcaagca taactttaat tctggttatt ctgataaaaa gtttctacaa catggagaag agatggaatt tacagggaac aatagaaatg atatattagg aagaaatgga aacttaatca gacaaaatat atcagaatat aaaaaaagcg ttggagtaat ggaagggagc tatgttgaca gaaacatgga gatttcagaa accccaagag taattcaaca gttagaaact aataaacgtc aaataagtga agaatttact gagaaaagag atcatgaata tgataccaga attgtagata agaacactaa attgagtagc aaaagtggat ctcaaagctc attgaaccaa acaaactaca agaaggtgaa cttgggagga tttactgaag cagattggta taaaaagatt ccaaattggg aaagaataat tgatgaaaga tatacaagcg tttatagttc atatgaaaga cttgaaatga taggaactgg gacttatgct aaagtttggc ttttgaaaga taaaataacg gggaagaaat atgcaggaaa actattagaa cctcatactt atccttcaga tacagtagaa aggattgaga gaatgtttca gtcagaaata aagaatctca tagtaagcca atgtccaggt gtagttaggc tgcacaagat cgtagtaggc ccagaaggaa gcctattagt tcaagattat gtagatgatg gtactatttg gcgtgagaac tgttgtgtaa gcgaattgga agccttccag cactttattc aactaattca gagcgtatta tacttccaag acaagggggt agtacataga gacttgaaac caacgaatat tttaagatat tcaaataaaa caatagtaat aggtgatttt gggtggagtg aacaaacgaa gaagttacat ttgagcccct cagaatggcc aggaacactt gaaattaatc ccccggaagt attgacattt aaaggtcctt taaccgaaaa gatagacaac tatgcagttg gaatgaactt attgttgttt ctcactggta ggtttatttg cagacagaaa ggattggatg ttacagaagc agctccttat attctaaaag tagtggaact aatcagaagt gattttaata ataagattct gagtaaagga aaacaaaaag ggaatactca attaaagcag agccagtact tcatgtggga gatctttatt gggtttactg accccaaccc aaatacaagg tggagtatta agaaggcttt gtttcaccca gattgtattc aacagctttt attttgtttc cataataact ttccaattct atggcatcct caggtaattg ctataatcaa atcaaatatt atacctaaca atgtgagctc tcaccaaact ttcatagaag atgagaaaca atattgtgaa gttggaaaac aaagtattgg tatgggaaag agttcaatgg caaacataaa tagtagtaat cagataaata ttcacaatgt ttcacatgct acacatttta ataattatag tggcggtcag agtccagttt ctgaagactt agcagaatcc cagagaagct attataagtc agttgggacg tctctaaggc ctgcaaatcc tccaataggt cacaagttct ttaaaaacga cggacaacaa cataaactaa tgctaaacag tcagtttttg tcagggtctt ctgataacaa ttcagtttat aataacgagt ttgctgcatc attaagaaac caacaacagg tgaatacctc ctctgcagcc actctaagtt atacaaacaa tggccataag tttgttgggg ttgaaagttc tgttgtgaag caaccccatt ttatcaataa ttttcactat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for CrpaA.01391.a.HZ1.GE41058
HARVESTED ON: 1/9/2017
SEQUENCED ON: 1/6/2017
EXPECTED MW: 90kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMY TIHEGCNISG NTRIYDTPNR HTWDINNGGT DGMRNESSTP RFKHNFNSGY SDKKFLQHGE EMEFTGNNRN DILGRNGNLI RQNISEYKKS VGVMEGSYVD RNMEISETPR VIQQLETNKR QISEEFTEKR DHEYDTRIVD KNTKLSSKSG SQSSLNQTNY KKVNLGGFTE ADWYKKIPNW ERIIDERYTS VYSSYERLEM IGTGTYAKVW LLKDKITGKK YAGKLLEPHT YPSDTVERIE RMFQSEIKNL IVSQCPGVVR LHKIVVGPEG SLLVQDYVDD GTIWRENCCV SELEAFQHFI QLIQSVLYFQ DKGVVHRDLK PTNILRYSNK TIVIGDFGWS EQTKKLHLSP SEWPGTLEIN PPEVLTFKGP LTEKIDNYAV GMNLLLFLTG RFICRQKGLD VTEAAPYILK VVELIRSDFN NKILSKGKQK GNTQLKQSQY FMWEIFIGFT DPNPNTRWSI KKALFHPDCI QQLLFCFHNN FPILWHPQVI AIIKSNIIPN NVSSHQTFIE DEKQYCEVGK QSIGMGKSSM ANINSSNQIN IHNVSHATHF NNYSGGQSPV SEDLAESQRS YYKSVGTSLR PANPPIGHKF FKNDGQQHKL MLNSQFLSGS SDNNSVYNNE FAASLRNQQQ VNTSSAATLS YTNNGHKFVG VESSVVKQPH FINNFHY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtatactat tcacgaaggg tgtaatatat cagggaacac aaggatttat gacactccga acagacacac ttgggatatc aataatggag gtactgatgg tatgaggaat gaaagttcaa cacctagatt caagcataac tttaattctg gttattctga taaaaagttt ctacaacatg gagaagagat ggaatttaca gggaacaata gaaatgatat attaggaaga aatggaaact taatcagaca aaatatatca gaatataaaa aaagcgttgg agtaatggaa gggagctatg ttgacagaaa catggagatt tcagaaaccc caagagtaat tcaacagtta gaaactaata aacgtcaaat aagtgaagaa tttactgaga aaagagatca tgaatatgat accagaattg tagataagaa cactaaattg agtagcaaaa gtggatctca aagctcattg aaccaaacaa actacaagaa ggtgaacttg ggaggattta ctgaagcaga ttggtataaa aagattccaa attgggaaag aataattgat gaaagatata caagcgttta tagttcatat gaaagacttg aaatgatagg aactgggact tatgctaaag tttggctttt gaaagataaa ataacgggga agaaatatgc aggaaaacta ttagaacctc atacttatcc ttcagataca gtagaaagga ttgagagaat gtttcagtca gaaataaaga atctcatagt aagccaatgt ccaggtgtag ttaggctgca caagatcgta gtaggcccag aaggaagcct attagttcaa gattatgtag atgatggtac tatttggcgt gagaactgtt gtgtaagcga attggaagcc ttccagcact ttattcaact aattcagagc gtattatact tccaagacaa gggggtagta catagagact tgaaaccaac gaatatttta agatattcaa ataaaacaat agtaataggt gattttgggt ggagtgaaca aacgaagaag ttacatttga gcccctcaga atggccagga acacttgaaa ttaatccccc ggaagtattg acatttaaag gtcctttaac cgaaaagata gacaactatg cagttggaat gaacttattg ttgtttctca ctggtaggtt tatttgcaga cagaaaggat tggatgttac agaagcagct ccttatattc taaaagtagt ggaactaatc agaagtgatt ttaataataa gattctgagt aaaggaaaac aaaaagggaa tactcaatta aagcagagcc agtacttcat gtgggagatc tttattgggt ttactgaccc caacccaaat acaaggtgga gtattaagaa ggctttgttt cacccagatt gtattcaaca gcttttattt tgtttccata ataactttcc aattctatgg catcctcagg taattgctat aatcaaatca aatattatac ctaacaatgt gagctctcac caaactttca tagaagatga gaaacaatat tgtgaagttg gaaaacaaag tattggtatg ggaaagagtt caatggcaaa cataaatagt agtaatcaga taaatattca caatgtttca catgctacac attttaataa ttatagtggc ggtcagagtc cagtttctga agacttagca gaatcccaga gaagctatta taagtcagtt gggacgtctc taaggcctgc aaatcctcca ataggtcaca agttctttaa aaacgacgga caacaacata aactaatgct aaacagtcag tttttgtcag ggtcttctga taacaattca gtttataata acgagtttgc tgcatcatta agaaaccaac aacaggtgaa tacctcctct gcagccactc taagttatac aaacaatggc cataagtttg ttggggttga aagttctgtt gtgaagcaac cccattttat caataatttt cactattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for CrpaA.01391.a.MB1.GE41056
HARVESTED ON: 1/9/2017
SEQUENCED ON: 1/6/2017
EXPECTED MW: 121kDa
OBSERVED MW: 121kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MYTIHEGCNI SGNTRIYDTP NRHTWDINNG GTDGMRNESS TPRFKHNFNS GYSDKKFLQH GEEMEFTGNN RNDILGRNGN LIRQNISEYK KSVGVMEGSY VDRNMEISET PRVIQQLETN KRQISEEFTE KRDHEYDTRI VDKNTKLSSK SGSQSSLNQT NYKKVNLGGF TEADWYKKIP NWERIIDERY TSVYSSYERL EMIGTGTYAK VWLLKDKITG KKYAGKLLEP HTYPSDTVER IERMFQSEIK NLIVSQCPGV VRLHKIVVGP EGSLLVQDYV DDGTIWRENC CVSELEAFQH FIQLIQSVLY FQDKGVVHRD LKPTNILRYS NKTIVIGDFG WSEQTKKLHL SPSEWPGTLE INPPEVLTFK GPLTEKIDNY AVGMNLLLFL TGRFICRQKG LDVTEAAPYI LKVVELIRSD FNNKILSKGK QKGNTQLKQS QYFMWEIFIG FTDPNPNTRW SIKKALFHPD CIQQLLFCFH NNFPILWHPQ VIAIIKSNII PNNVSSHQTF IEDEKQYCEV GKQSIGMGKS SMANINSSNQ INIHNVSHAT HFNNYSGGQS PVSEDLAESQ RSYYKSVGTS LRPANPPIGH KFFKNDGQQH KLMLNSQFLS GSSDNNSVYN NEFAASLRNQ QQVNTSSAAT LSYTNNGHKF VGVESSVVKQ PHFINNFHY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgta tactattcac gaagggtgta atatatcagg gaacacaagg atttatgaca ctccgaacag acacacttgg gatatcaata atggaggtac tgatggtatg aggaatgaaa gttcaacacc tagattcaag cataacttta attctggtta ttctgataaa aagtttctac aacatggaga agagatggaa tttacaggga acaatagaaa tgatatatta ggaagaaatg gaaacttaat cagacaaaat atatcagaat ataaaaaaag cgttggagta atggaaggga gctatgttga cagaaacatg gagatttcag aaaccccaag agtaattcaa cagttagaaa ctaataaacg tcaaataagt gaagaattta ctgagaaaag agatcatgaa tatgatacca gaattgtaga taagaacact aaattgagta gcaaaagtgg atctcaaagc tcattgaacc aaacaaacta caagaaggtg aacttgggag gatttactga agcagattgg tataaaaaga ttccaaattg ggaaagaata attgatgaaa gatatacaag cgtttatagt tcatatgaaa gacttgaaat gataggaact gggacttatg ctaaagtttg gcttttgaaa gataaaataa cggggaagaa atatgcagga aaactattag aacctcatac ttatccttca gatacagtag aaaggattga gagaatgttt cagtcagaaa taaagaatct catagtaagc caatgtccag gtgtagttag gctgcacaag atcgtagtag gcccagaagg aagcctatta gttcaagatt atgtagatga tggtactatt tggcgtgaga actgttgtgt aagcgaattg gaagccttcc agcactttat tcaactaatt cagagcgtat tatacttcca agacaagggg gtagtacata gagacttgaa accaacgaat attttaagat attcaaataa aacaatagta ataggtgatt ttgggtggag tgaacaaacg aagaagttac atttgagccc ctcagaatgg ccaggaacac ttgaaattaa tcccccggaa gtattgacat ttaaaggtcc tttaaccgaa aagatagaca actatgcagt tggaatgaac ttattgttgt ttctcactgg taggtttatt tgcagacaga aaggattgga tgttacagaa gcagctcctt atattctaaa agtagtggaa ctaatcagaa gtgattttaa taataagatt ctgagtaaag gaaaacaaaa agggaatact caattaaagc agagccagta cttcatgtgg gagatcttta ttgggtttac tgaccccaac ccaaatacaa ggtggagtat taagaaggct ttgtttcacc cagattgtat tcaacagctt ttattttgtt tccataataa ctttccaatt ctatggcatc ctcaggtaat tgctataatc aaatcaaata ttatacctaa caatgtgagc tctcaccaaa ctttcataga agatgagaaa caatattgtg aagttggaaa acaaagtatt ggtatgggaa agagttcaat ggcaaacata aatagtagta atcagataaa tattcacaat gtttcacatg ctacacattt taataattat agtggcggtc agagtccagt ttctgaagac ttagcagaat cccagagaag ctattataag tcagttggga cgtctctaag gcctgcaaat cctccaatag gtcacaagtt ctttaaaaac gacggacaac aacataaact aatgctaaac agtcagtttt tgtcagggtc ttctgataac aattcagttt ataataacga gtttgctgca tcattaagaa accaacaaca ggtgaatacc tcctctgcag ccactctaag ttatacaaac aatggccata agtttgttgg ggttgaaagt tctgttgtga agcaacccca ttttatcaat aattttcact attaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa